The photosynthetic purple sulfur bacteria were isolated from lakes Yavorivske (Lviv region) and Sakske (AR Crimea). The purple sulfur bacteria use reduced sulfur compounds as electron donors for anoxygenic photosynthesis. The elemental sulfur produced by these microorganisms as intermediate in the oxidation of thiosulfate or hydrogen sulfide to sulfate. The bacteria strains Ya 2008/С and Sa 2008 / D with highest intracellular sulfur content have been choose for next investigation. Their morphophysiology characteristics, pigment content, the resistances to hydrogen sulfide, utilization of H 2 S and intracellular sulfur during photolitoautotrophic growth were investigated. Strains Ya 2008/С and Sa 2008 / D according to their morphophysiology characteristics were identified to genus Chromatium . Keywords : purple sulfur bacteria, Chromatium , intracellular sulfur, carotenoids, bacteriochlorophyll a .