Annuum Cultivars Research Articles

Evaluation of chemical profile and antioxidant activity of twenty cultivars from Capsicum annuum, Capsicum baccatum, Capsicum chacoense and Capsicum chinense: A comparison between fresh and processed peppers

Recently, much attention has been directed to the micronutrient content of processed foods. In the present study total phenols, flavonoids, capsaicinoids and antioxidant activity were determined on fresh, boiled and frozen chili peppers cultivars belonging to four Capsicum species (Capsicum annuum, Capsicum baccatum, Capsicum chacoense and C. chinense). The obtained data showed that fresh peppers exhibited the highest phenols, flavonoids and capsaicinoids content. Boiling and freezing process negatively influenced the content of these phytochemicals. Generally, C. annuum cultivars have the greater antioxidant activity relatable to the higher phenols and capsaicinoids content. Considering the attention of consumers in respect of the nutritional properties of the food, it seems necessary to address more carefully the effects of food home processing on the healthy compounds.

Difference in root-to-shoot Cd translocation and characterization of Cd accumulation during fruit development in two Capsicum annuum cultivars

Cultivars of hot pepper (Capsicum annuum L.) vary greatly in their fruit cadmium (Cd) concentration. Previously, we identified a low-Cd (YCT) and a high-Cd (JFZ) cultivar. In this study, we elucidated the physiological mechanisms resulting in the differences in their Cd accumulation. A time-dependent and concentration-dependent hydroponic experiment was conducted to investigate the difference in root-to-shoot Cd translocation between the two cultivars. Furthermore, a pot experiment was carried out to determine the Cd accumulation in all plant tissues of the two cultivars during fruit development. A greater and more rapid root-to-shoot Cd translocation was observed in JFZ. Moreover, the Cd continuously increased in all plant tissues during fruit development, but following fruit development, the fruit Cd concentration decreased in both cultivars. In each cultivar, the Cd accumulated in the fruit was significantly positively correlated with fruit dry weight. JFZ has a higher ability in Cd translocation from roots to shoots via the xylem. The positive correlation between fruit biomass and Cd content supports that the phloem is the major route for Cd loading into developing hot pepper fruits.

Virulence of Spanish Phytophthora nicotianae isolates towards Capsicum annuum germplasm and pathogenicity towards Lycopersicum esculentum

The virulence of six northwestern Spanish Phytophthora nicotianae Breda de Haan isolates towards the pepper (Capsicum annuum) cultivars commonly used for the determination of P. capsici Leonian pathotype (SCM 334, PI201234 and Yolo Wonder), was similar to that of the latter oomycete but different to that of the German reference isolate P. nicotianae 411.87. When nine local C. annuum cultivars were inoculated with a P. nicotianae isolate of well known pathogenicity, the virulence recorded was significantly different to that recorded for P. capsici. Though the average level of resistance of the northwestern Spanish Capsicum germplasm to P. nicotianae was incomplete and weaker than that recorded for the SCM 334 and PI201234 cultivars, genotypes Co 12B and Co 3.25 showed high resistance to this oomycete. None of the six northwestern Spanish P. nicotianae isolates tested were pathogenic towards Lycopersicum esculentum cv. S. Pedro, unlike P. capsici, which produced clear collar and root rot in this host.

In vitro investigation of the bioaccessibility of carotenoids from raw, frozen and boiled red chili peppers (Capsicum annuum)

Carotenoid-rich foods are associated with antioxidant activity and the ability to alleviate chronic diseases. The present study investigated the effect of processing on the content and bioaccessibility of carotenoids from 13 cultivars of red chili pepper (Capsicum annuum). Carotenoids in chili peppers were analyzed before an in vitro digestion process. The portion of carotenoid transferred to the micelle fraction (bioaccessibility) was also quantified. β-Carotene, β-cryptoxanthin, capsanthin and antheraxanthin were the most abundant carotenoids. Zeaxanthin, violaxanthin, neoxanthin and lutein were detected at lower concentrations. In general, freezing and boiling reduced carotenoid contents. Capsanthin and zeaxanthin had the highest bioaccessibility at an average value from 36 to 40%, followed by antheraxanthin (26%). Bioaccessibility of β-cryptoxanthin, violaxanthin and β-carotene was lower, averaging 6.1, 4.8 and 4.0%, respectively. Neoxanthin and lutein were not detected in micelles. Freezing increased the bioaccessibility of capsanthin, zeaxanthin, antheraxanthin, β-cryptoxanthin and violaxanthin; β-cryptoxanthin bioaccessibility increased and capsanthin and zeaxanthin bioaccessibility decreased following boiling. Differences in the contents and bioaccessibility of carotenoids in 13 C. annuum cultivars and between the processed methods were herein evidenced.

Antioxidant and hypoglycaemic activities and their relationship to phytochemicals in Capsicum annuum cultivars during fruit development

Four Capsicum annuum L. cultivars (Fiesta, Acuminatum, Orange Thai, and Cayenne Golden) were studied at two stages of fruits ripening (immature and mature) for total phenols, flavonoids, carotenoids, capsaicin and dihydrocapsaicin content and for their antioxidant and hypoglycaemic activities. Phenols content reached their maximum concentration in immature fruits and then declined, while carotenoids and capsaicinoids concentration increased as the peppers reached maturity. The antioxidant activity was elucidated by DPPH and β-carotene bleaching tests. Except for Cayenne Golden cultivar, the immature fruits showed the highest radical scavenging activity. On the contrary, the antioxidant activity evaluated by the β-carotene bleaching test showed a significant activity for mature peppers. The Cayenne Golden cultivar showed an IC50 value of 2.5 μg/ml after 30 min of incubation. Hypoglycaemic effects were examined in vitro via the inhibition of α-amylase and α-glucosidase enzymes. Fiesta, Orange Thai, and Cayenne Golden cultivars showed the highest inhibitory activity on α-amylase in the immature stage. The lipophilic fraction exhibited a selective inhibitory activity against α-amylase with IC50 values ranging from 9.1 μg/ml to 28.6 μg/ml in the immature stage. The study proposes the nutritional significance of consuming C. annuum raw peppers at both maturity stages because of interesting properties.

Phenotypic and genotypic variation among Capsicum annuum recombinant inbred lines resistant to bacterial spot

A breeding program carried out under Brazilian growing conditions to obtain Capsicum annuum cultivars with disease resistance to bacterial spot (BS) produced 8 promising recombinant inbred lines (RILs). The present study aimed to characterize these RILs using phenotypic descriptors and molecular markers (inter-simple sequence repeat) and to confirm their resistance to BS. Twenty-two phenotypic descriptors and 15 inter-simple sequence repeat primers were used to characterize the RILs. The parent, UENF 1381, which is resistant to BS, and 'Casca Dura Ikeda', a traditional cultivar, were used as standards. Variability among genotypes was observed considering either binary or multicategorical characteristics, such as fruit length, fruit diameter, and fruit longitudinal and transversal section. Such variability in fruit traits can be exploited to develop new genotypes with BS resistance for various types of market consumption. RILs numbered 1, 3, and 6 were the most homogenous, whereas those coded 2, 5, 8, and 11 had the same level of heterogeneity as that observed in 'Casca Dura Ikeda'. Molecular analysis clustered the genotypes into 5 groups, with RILs 1, 2, 3, and 5 allocated in isolated groups. RILs 1, 2, 6, and 8 confirmed resistance to BS. Considering homogeneity level and BS resistance, RILs 1 and 6 were suitable for use as pre-cultivars in final tests to register and release two new C. annuum cultivars.

Colletotrichum truncatum Pathosystem on Capsicum spp: infection, colonization and defence mechanisms

The disease cycle of the chili (Capsicum annuum) anthracnose fungus Colletotrichum truncatum (formerly C. capsici) was elucidated from a study of infection and colonization of seed, leaves and fruit. Microscopic observations of detached leaves and fruit inoculated with a virulent pathotype (F83B), revealed direct cuticle penetration and, intramural, endophytic and necrotrophic phases of colonization. Seedling and fruit ripening stages were very susceptible to infection with the pathogen causing pre- and post-emergence damage and postharvest fruit rot. Furthermore, a quiescent stage, following leaf infection during the vegetative phase of plant growth served as a potential primary inoculum source for fruit infection. Leaf epidermal cells of the resistant C. chinense PBC932 expressed a strong hypersensitive response 48 h after infection (HAI) to both highly virulent (F83B) and less virulent (BRIP 26,974) pathotypes. Infected cells had thickened cell walls, cytoplasm aggregation, and high levels of reactive oxygen species produced 12 HAI. In contrast, the infected epidermal cells of the susceptible C. annuum cultivar Bangchang showed necrosis and rapid cell death after infection by either pathotype. Knowledge of the disease cycle of C. truncatum will be helpful in understanding the behaviour of the pathogen in chili fields which will lead to more efficient application of control measures.

Air-dried capsicum annuum var. acuminatum medium and big: Determination of bioactive constituents, antioxidant activity and carbohydrate-hydrolyzing enzymes inhibition

In the present study the total phenols, flavonoids, carotenoids and capsaicinoids content, the in vitro antioxidant and hypoglycemic activities of extracts of air-dried fruits from two Capsicum annuum cultivars were investigated. A different composition between the two cultivars was evidenced. C. annuum var. acuminatum medium extract presented a major content of phenols, carotenoids, capsaicin and dihydrocapsaicin while C. annuum var. acuminatum big extract is characterized by the highest quercetin, luteolin and kaempferol content with 68.0, 87.6 and 29.7μg/g dried weight, respectively, analyzed by HPLC. Medium pepper showed the highest radical scavenging activity in DPPH assay (IC50 of 85.3μg/ml) while big pepper have an interesting activity in ABTS assay (IC50 of 16.4μg/ml) and the highest inhibition of linoleic acid oxidation with an IC50 value of 1.2μg/ml after 30min of incubation. A selective inhibitory activity against α-amylase was demonstrated for C. annuum var. acuminatum big lipophilic fraction (IC50 values of 8.7μg/ml). The obtained results suggest that C. annuum cultivars could be used as valuable flavor with functional properties for foods.

Carotenoid accumulation in orange-pigmented Capsicum annuum fruit, regulated at multiple levels

The pericarp of Capsicum fruit is a rich dietary source of carotenoids. Accumulation of these compounds may be controlled, in part, by gene transcription of biosynthetic enzymes. The carotenoid composition in a number of orange-coloured C. annuum cultivars was determined using HPLC and compared with transcript abundances for four carotenogenic enzymes, Psy, LcyB, CrtZ-2, and Ccs determined by qRT-PCR. There were unique carotenoid profiles as well as distinct patterns of transcription of carotenogenic enzymes within the seven orange-coloured cultivars. In one cultivar, ‘Fogo’, carrying the mutant ccs-3 allele, transcripts were detected for this gene, but no CCS protein accumulated. The premature stop termination in ccs-3 prevented expression of the biosynthetic activity to synthesize the capsanthin and capsorubin forms of carotenoids. In two other orange-coloured cultivars, ‘Orange Grande’ and ‘Oriole’, both with wild-type versions of all four carotenogenic enzymes, no transcripts for Ccs were detected and no red pigments accumulated. Finally, in a third case, the orange-coloured cultivar, Canary, transcripts for all four of the wild-type carotenogenic enzymes were readily detected yet no CCS protein appeared to accumulate and no red carotenoids were synthesized. In the past, mutations in Psy and Ccs have been identified as the loci controlling colour in the fruit. Now there is evidence that a non-structural gene may control colour development in Capsicum.

Identification of Novel Physiological Races of Phytophthora capsici Causing Foliar Blight Using the New Mexico Recombinant Inbred Pepper Lines Set as a Host Differential

Phytophthora foliar blight caused by Phytophthora capsici is a serious limitation to pepper (Capsicum annuum) production worldwide. Knowledge of the physiological race composition of isolates causing phytophthora foliar blight is necessary for success in breeding for disease resistance. The New Mexico recombinant inbred lines (NMRILs) effectively differentiated isolates from different geographical locations (i.e., Turkey, The Netherlands, Argentina, and two states in the United States) into 12 physiological races of P. capsici causing phytophthora foliar blight. This research demonstrates the use of the NMRILs to identify P. capsici foliar blight races and the importance of identifying the physiological races occurring in specific regions where a C. annuum cultivar will be grown. Knowing the physiological race(s) in a region will provide valuable information to formulate breeding strategies to deploy durable foliar blight resistance.

Novel Loss-of-Functionputative aminotransferaseAlleles Cause Biosynthesis of Capsinoids, Nonpungent Capsaicinoid Analogues, in Mildly Pungent Chili Peppers (Capsicum chinense)

Capsinoids are a group of nonpungent capsaicinoid analogues produced in Capsicum fruits. They have similar bioactivities to capsaicinoids such as suppression of fat accumulation and antioxidant activity. They are more palatable ingredients in dietary supplements than capsaicinoids because of their low pungency. Previous studies on nonpungent Capsicum annuum cultivars showed that capsinoid biosynthesis is caused by loss-of-function putative aminotransferase (p-amt) alleles. This study showed that three mildly pungent cultivars of Capsicum chinense (Zavory Hot, Aji Dulce strain 2, and Belize Sweet) contain high levels of capsinoid. It was shown that these cultivars have novel p-amt alleles, which contain mutations that differ from those of C. annuum. Sequence analysis of p-amt in Belize Sweet revealed that a 5 bp insertion (TGGGC) results in a frameshift mutation. A transposable element (Tcc) was found in the p-amt of Zavory Hot and Aji Dulce strain 2. Tcc has features similar to those of the hAT transposon family. This was inserted in the fifth intron of Zavory Hot and in third intron of Aji Dulce strain 2. The p-amt alleles harboring Tcc cannot produce an active p-AMT. These mildly pungent cultivars will provide a new natural source of capsinoids.

Development of a Double‐inoculation Method to Assess Resistance to Anthracnose in Trispecies Capsicum Hybrid

AbstractChilli anthracnose is caused by a complex of Colletotrichum species. Breeding for resistance to anthracnose has been focused on introgressing resistance from Capsicum chinense and C. baccatum into commercial C. annuum cultivars. Trispecies hybrids of C. annuum cv. Bangchang, C. chinense PBC932 and C. baccatum PBC80 were successfully produced. Assessments for resistance in F1 progeny to Colletotrichum capsici isolate 158ci (Cc158ci) and C. acutatum isolate MJ5 (CaMJ5) were carried out by inoculating fruit using a laboratory microinjection method. Due to the poor fruit set of the F1 hybrid, a double‐inoculation method was developed to inoculate the same chilli fruit with two isolates of two Colletotrichum species. The positions (apex, centre, end) at which the fruit were inoculated with either isolate did not affect disease development. At 7 days after inoculation, Cc158ci produced larger lesions on chilli fruit than CaMJ5; and lesions from single inoculation were larger than those from double inoculation. The double‐inoculation technique was applied to the trispecies F1 hybrid to select individual F1 plants that contained resistance to both Colletotrichum species. Of the nine F1 plants that produced fruits for inoculation, all were resistant to Cc158ci at both mature green and ripe fruit stages. Two plants were also resistant to CaMJ5 at both fruit maturity stages, and one plant was resistant at the ripe fruit stage but susceptible at the green fruit stage.

Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.)

Cucumber mosaic virus (CMV) is one of the most destructive viruses in the Solanaceae family. Simple inheritance of CMV resistance in peppers has not previously been documented; all previous studies have reported that resistance to this virus is mediated by several partially dominant and recessive genes. In this study, we showed that the Capsicum annuum cultivar 'Bukang' contains a single dominant resistance gene against CMV(Korean) and CMV(FNY) strains. We named this resistance gene Cmr1 (Cucumber mosaic resistance 1). Analysis of the cellular localization of CMV using a CMV green fluorescent protein construct showed that in 'Bukang,' systemic movement of the virus from the epidermal cell layer to mesophyll cells is inhibited. Genetic mapping and FISH analysis revealed that the Cmr1 gene is located at the centromeric region of LG2, a position syntenic to the ToMV resistance locus (Tm-1) in tomatoes. Three SNP markers were developed by comparative genetic mapping: one intron-based marker using a pepper homolog of Tm-1, and two SNP markers using tomato and pepper BAC sequences mapped near Cmr1. We expect that the SNP markers developed in this study will be useful for developing CMV-resistant cultivars and for fine mapping the Cmr1 gene.

The use of induced mutations to study shoot architecture in <i>Capsicum</i>

Towards our goal to produce a saturated mutation library of pepper as a tool to study pepper development, we generated an EMS mutant collection of 1650 M2 families of the blocky Capsicum annuum cultivar Maor. M2 families were evaluated for changes in characters related to shoot architecture. The main categories of mutations, which included changes in sympodial growth, leaf structure, and plant height, are described. Some of the mutant phenotypes were very frequent (e.g., 10% of all mutants were affected in plant height), while others were found only once. This difference in mutation rate may reflect varying complexities of genetic control for different traits or different rates of mutability in different genes. By utilizing phenotypic similarities with known mutants of model species, we will be able to test candidate genes for controlling the pepper mutations. This will allow comparing gene function within the Solanaceae family and across more diverse plant species, and will increase understanding of the ...

Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

Real-time PCR was used to detect and quantify Verticillium dahliae and to assess the susceptibility of four Capsicum annuum cultivars (Luesia, Padron, SCM331 and PI201234) and the Capsicum chinense cv. C118 to this pathogen. The symptoms which developed after infection included stunting and yellowing, and were more acute in the cv. SCM331, which also suffered defoliation in later stages of the disease and in C118, which suffered severe stunting. Quantification of the pathogen DNA in roots 23 and 34 days post-inoculation (dpi) revealed that there were significantly higher amounts of Verticillium dahliae DNA in C118 than in the other cultivars, followed by SCM331, Padron and PI201234. The lowest amounts of fungal DNA in roots were found in Luesia. In hypocotyls, the highest amounts of fungal DNA were found in SCM331, while Luesia, Padron and PI201234 had much lower amounts, and C118 had intermediate levels. When a compatible versus an incompatible system was studied, using the near-isogenic tomato lines LA3030 (susceptible) and LA3038 (resistant to V. dahliae), we were able to detect fungal DNA in both lines. As expected, the fungus/plant DNA ratio was lower in LA3038 than in LA3030 and it decreased with time in LA3038. The amount of Verticillium dahliae DNA in the roots of LA3030 remained constant between days 23 and 34 post-inoculation, but increased 10-fold in collars. Finally, when real-time PCR was applied as a diagnostic method to samples from pepper plants, soil and water collected from farms in northwest Spain, we were able to detect V. dahliae DNA in these samples even when symptoms of the disease were not evident.

Assessment of carotenoids, capsaicinoids and ascorbic acid composition of some selected pepper cultivars ( Capsicum annuum L.) grown in Turkey

Carotenoid, capsaicinoid and ascorbic acid composition of ripe fruits of five Capsicum annuum cultivars (730 F1, 1245 F1, Amazon F1, Serademre 8 and Kusak 295F1), grown as principle breeding material in Turkey, were quantitatively investigated by means of HPLC technique. Seven main carotenoids, five analogues of capsaicinoids and ascorbic acid were quantified in the fruits grown for 2 year replication. From the capsaicinoids and carotenoids data, Scoville Heat Unit (SHU) and retinol activity equivalent (RAE) values of the fruits were also calculated, respectively. The findings determined that the cultivars of 730 F1 and 1245 F1 had higher carotenoids (2310–2390 mg/kg in dry basis), capsaicinoids (471.3–688.1 mg/kg in dry basis), vitamin A (218.8–243.0 μg RAE/100 g in wet basis) and vitamin C (63.1–64.9 mg/100 g in wet basis) content, without any significant difference among each of them. Furthermore, the cultivars which had higher capsaicinoids contents had higher ascorbic acids content as well. With their high nutritional and functional components, the cultivar of 730 F1 and 1245 F1 can be considered to be selected breeding material for cultivar development.

Purification and Structure Determination of Glucosides of Capsaicin and Dihydrocapsaicin from Various Capsicum Fruits

Two new glucosides, capsaicin-beta-D-glucopyranoside (1) and dihydrocapsaicin-beta-D-glucopyranoside (2), were discovered in the fruit of the Capsicum annuum cultivar 'High Heat'. They were sequentially purified by acetone extraction, n-hexane extraction, and acetonitrile extraction, followed by medium-pressure liquid chromatography and high-performance liquid chromatography performed on an octadecylsilane column. Their chemical structures were elucidated by proton nuclear magnetic resonance, carbon nuclear magnetic resonance, and hydrolysis with alpha- and beta-glucosidases. The glucosides were also detected in various pungent cultivars of C. annuum, Capsicum frutescens, and Capsicum chinense by liquid chromatography-mass spectrometry. However, they were not detected in nonpungent cultivars of C. annuum. Furthermore, a positive correlation was observed between the quantity of the capsaicinoids, capsaicin, and dihydrocapsaicin and their glucosides.

Seed Storage Proteins in Solanaceae and Cucurbitaceae Species

Electrophoretic analyses of non-reduced and reduced seed storage proteins from Solanaceae and Cucurbitaceae species and cultivars were performed. High molecular disulfide bonded complexes between intermediary subunits of 11S globulins previously detected in Capsicum annuum cultivars, were found in Solanum melongena cultivars as well. The data obtained might be used for further elucidation of peculiarities of the 11S globulins in dicotyledonous plants.

Changes in stem lignins (monomer composition and crosslinking) and peroxidase are related with the maintenance of leaf photosynthetic integrity during Verticillium wilt in Capsicum annuum.

• Verticillium dahliae is a soilborne pathogen that causes vascular wilt in pepper (Capsicum annuum var. annuum). Here we study to what extent changes in the lignification response of peppers condition tolerance of wilt. • For this, the quantum yield (ΦPSII ), the linear electron transport rate (ETR), and the lignification response (monomer composition and crosslinking) were studied in three C. annuum cultivars differing in degree of tolerance. • The results showed that in tolerant cultivars (Padrón and Yolo Wonder), both ΦPSII and ETR showed significantly higher levels at saturating photosynthetically active radiation values. This was not, however, the case for cv. Luesia, which showed a significant decrease in ΦPSII , ETR and nonphotochemical quenching values, suggesting that photochemical processes are strongly damaged in this cultivar as a consequence of the disease. The analysis of stem lignins in tolerant cultivars revealed that they were mainly composed of p-hydroxyphenyl units, while levels of β-O-4-linked coniferyl and sinapyl alcohols were significantly lower. • It is concluded that through the observed changes in stem lignins (monomer composition and crosslinking) peppers retard, since they maintain leaf photosynthetic integrity, but do not stop (since wilt symptoms are not avoided) V. dahliae fungal hyphae penetration.

Molecular AFLP Analysis of the Genotypes of Pepper Capsicum annuum Cultivars

The results of AFLP study of 14 Capsicum annuum cultivars are presented. In spite of the known low genomic variation of large-fruited sweet pepper, AFLP analysis proved to be suitable for detecting polymorphism and genotyping pepper cultivars. Nine primer pairs were selected to allow identification of the cultivars under study. Among-cultivar polymorphism detectable with these primers was estimated at 16.5%. A characteristic AFLP pattern was obtained for each cultivar. Several cultivar-specific fragments were revealed for seven cultivars. On the basis of the AFLP data, genetic distances between cultivars were determined and a tree was constructed by means of hierarchic cluster analysis (UPGMA) with the Jacquard coefficient. It was assumed that this information is useful in breeding programs involving the cultivars examined.