Sustainable management strategies that facilitate the incorporation of trees into cattle pastures are widely recognized as emerging natural climate solutions. However, their potential to dually improve ecological functioning and human livelihoods has yet to be evaluated across regions on local scales, utilizing both ecological and sociological data. Further, the relationship between landowner participation in conservation management programs (CMPS) and their management priorities (i.e. production vs sustainability) also remains unclear. Therefore, the aims of this study were to evaluate the accuracy of past global-scale remote estimations of aboveground biomass and derived tree carbon density (MgC ha−1) within pasturelands at the local scale in both temperate and tropical regions. We also sought to analyze how participation in CMPs related to the perceptions of stakeholders toward the ecological health of their system. We compared findings between farms in temperate (n = 26) and tropical (n = 16) ecosystems. We found that discrepancies exist between previous remote studies and current field estimations of tree carbon in the farms, and these differences were not regionally uniform. In contrast, stakeholders enrolled in CMPs had a higher value of trees in both regions. CMPs participants also had lower annual stocking rates than those that practiced conventional management but no differences in woody species diversity (H’) or tree carbon (MgC ha−1). We demonstrate the urgent need for local-scale studies to accurately estimate tree carbon in mosaic pasture systems and the importance of conservation management in shifting the priorities of landowners, which could eventually lead to more sustainable systems.