Glacier meltwater is an indispensable water supply for billions of people living in the catchments of major Asian rivers. However, the role of glaciers on river runoff regulation is seldom investigated due to the lack of annual glacier mass balance observation. In this study, we employed an albedo-based model with a daily land surface albedo dataset to derive the annual glacier mass balance over the Tuotuo River Basin (TRB). During 2000–2022, an annual glacier mass balance range of −0.89 ± 0.08 to 0.11 ± 0.11 m w.e. was estimated. By comparing with river runoff records from the hydrometric station, the contribution of glacier mass change to river runoff was calculated to be 0.00–31.14% for the studied period, with a mean value of 9.97%. Moreover, we found that the mean contribution in drought years is 20.07%, which is approximately five times that in wet years (4.30%) and twice that in average years (9.49%). Therefore, our results verify that mountain glaciers act as a significant buffer against drought in the TRB, at least during the 2000–2022 period.
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