Annotation Method Research Articles

IDet3D: Towards Efficient Interactive Object Detection for LiDAR Point Clouds

Accurately annotating multiple 3D objects in LiDAR scenes is laborious and challenging. While a few previous studies have attempted to leverage semi-automatic methods for cost-effective bounding box annotation, such methods have limitations in efficiently handling numerous multi-class objects. To effectively accelerate 3D annotation pipelines, we propose iDet3D, an efficient interactive 3D object detector. Supporting a user-friendly 2D interface, which can ease the cognitive burden of exploring 3D space to provide click interactions, iDet3D enables users to annotate the entire objects in each scene with minimal interactions. Taking the sparse nature of 3D point clouds into account, we design a negative click simulation (NCS) to improve accuracy by reducing false-positive predictions. In addition, iDet3D incorporates two click propagation techniques to take full advantage of user interactions: (1) dense click guidance (DCG) for keeping user-provided information throughout the network and (2) spatial click propagation (SCP) for detecting other instances of the same class based on the user-specified objects. Through our extensive experiments, we present that our method can construct precise annotations in a few clicks, which shows the practicality as an efficient annotation tool for 3D object detection.

FT-GAN: Fine-Grained Tune Modeling for Chinese Opera Synthesis

Although singing voice synthesis (SVS) has made significant progress recently, with its unique styles and various genres, Chinese opera synthesis requires greater attention but is rarely studied for lack of training data and high expressiveness. In this work, we build a high-quality Gezi Opera (a type of Chinese opera popular in Fujian and Taiwan) audio-text alignment dataset and formulate specific data annotation methods applicable to Chinese operas. We propose FT-GAN, an acoustic model for fine-grained tune modeling in Chinese opera synthesis based on the empirical analysis of the differences between Chinese operas and pop songs. To further improve the quality of the synthesized opera, we propose a speech pre-training strategy for additional knowledge injection. The experimental results show that FT-GAN outperforms the strong baselines in SVS on the Gezi Opera synthesis task. Extensive experiments further verify that FT-GAN performs well on synthesis tasks of other operas such as Peking Opera. Audio samples, the dataset, and the codes are available at https://zhengmidon.github.io/FTGAN.github.io/.

Text2Analysis: A Benchmark of Table Question Answering with Advanced Data Analysis and Unclear Queries

Tabular data analysis is crucial in various fields, and large language models show promise in this area. However, current research mostly focuses on rudimentary tasks like Text2SQL and TableQA, neglecting advanced analysis like forecasting and chart generation. To address this gap, we developed the Text2Analysis benchmark, incorporating advanced analysis tasks that go beyond the SQL-compatible operations and require more in-depth analysis. We also develop five innovative and effective annotation methods, harnessing the capabilities of large language models to enhance data quality and quantity. Additionally, we include unclear queries that resemble real-world user questions to test how well models can understand and tackle such challenges. Finally, we collect 2249 query-result pairs with 347 tables. We evaluate five state-of-the-art models using three different metrics and the results show that our benchmark presents introduces considerable challenge in the field of tabular data analysis, paving the way for more advanced research opportunities.

ExpCLIP: Bridging Text and Facial Expressions via Semantic Alignment

The objective of stylized speech-driven facial animation is to create animations that encapsulate specific emotional expressions. Existing methods often depend on pre-established emotional labels or facial expression templates, which may limit the necessary flexibility for accurately conveying user intent. In this research, we introduce a technique that enables the control of arbitrary styles by leveraging natural language as emotion prompts. This technique presents benefits in terms of both flexibility and user-friendliness. To realize this objective, we initially construct a Text-Expression Alignment Dataset (TEAD), wherein each facial expression is paired with several prompt-like descriptions. We propose an innovative automatic annotation method, supported by CahtGPT, to expedite the dataset construction, thereby eliminating the substantial expense of manual annotation. Following this, we utilize TEAD to train a CLIP-based model, termed ExpCLIP, which encodes text and facial expressions into semantically aligned style embeddings. The embeddings are subsequently integrated into the facial animation generator to yield expressive and controllable facial animations. Given the limited diversity of facial emotions in existing speech-driven facial animation training data, we further introduce an effective Expression Prompt Augmentation (EPA) mechanism to enable the animation generator to support unprecedented richness in style control. Comprehensive experiments illustrate that our method accomplishes expressive facial animation generation and offers enhanced flexibility in effectively conveying the desired style.

Abstract 2859: The Single-cell Pediatric Cancer Atlas: Open-source data and tools for single-cell transcriptomics of pediatric tumors

Abstract The Single-cell Pediatric Cancer Atlas (ScPCA) Portal (https://scpca.alexslemonade.org/), developed and maintained by the Childhood Cancer Data Lab, is an open-source data resource for single-cell and single-nuclei RNA sequencing data of pediatric tumors. Originally comprised of data from 10 projects funded by Alex’s Lemonade Stand Foundation, the Portal currently contains summarized gene expression data for over 500 samples from a diverse set of over 50 types of cancers and is growing to include community-contributed datasets. In addition to gene expression data from single-cell and single-nuclei RNA sequencing, the Portal holds data obtained from bulk RNA sequencing, spatial transcriptomics, and feature barcoding methods, such as CITE-seq and cell hashing. ScPCA data are available for download in formats ready for downstream analysis, such as SingleCellExperiment or AnnData objects. Objects include raw counts and normalized gene expression data, PCA and UMAP coordinates, unsupervised clustering assignments, and cell type annotations. Additionally, all downloads include two summary reports for each library. The quality control report is a general overview, including processing information, summary statistics, and general visualizations of cell metrics. The cell type annotation report includes an overview of cell type annotation, comparisons among cell type annotation methods, and diagnostic plots to assess annotation quality. Comprehensive documentation about data processing and the contents of files on the portal, including a guide to getting started working with an ScPCA dataset, can be found at scpca.readthedocs.io. All data on the Portal were uniformly processed using scpca-nf, a Nextflow-based open-source pipeline developed by the Childhood Cancer Data Lab. The scpca-nf workflow uses alevin-fry for fast and efficient processing of all data currently available on the portal, including single-cell RNA-seq data and any associated CITE-seq or cell hash data, spatial transcriptomics data, and bulk RNA sequencing. The workflow and associated documentation are freely available at https://github.com/AlexsLemonade/scpca-nf, allowing researchers to leverage this pipeline for their own datasets. Providing an open-source workflow has allowed researchers to process their own single-cell or single-nuclei datasets for their own research. Furthermore, any data sets processed with scpca-nf are then eligible for inclusion on the ScPCA Portal. The continuous growth of the ScPCA Portal will help pediatric cancer researchers spend less time finding and processing data and more time answering their pressing research questions. Citation Format: Allegra G. Hawkins, Joshua A. Shapiro, Stephanie J. Spielman, David S. Mejia, Deepashree Venkatesh Prasad, Nozomi Ichihara, Arkadii Yakovets, Kurt G. Wheeler, Chanté J. Bethell, Steven M. Foltz, Jennifer O'Malley, Casey S. Greene, Jaclyn N. Taroni. The Single-cell Pediatric Cancer Atlas: Open-source data and tools for single-cell transcriptomics of pediatric tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2859.

Robust chromatin state annotation.

With the goal of mapping genomic activity, international projects have recently measured epigenetic activity in hundreds of cell and tissue types. Chromatin state annotations produced by segmentation and genome annotation (SAGA) methods have emerged as the predominant way to summarize these epigenomic data sets in order to annotate the genome. These chromatin state annotations are essential for many genomic tasks, including identifying active regulatory elements and interpreting disease-associated genetic variation. However, despite the widespread applications of SAGA methods, no principled approach exists to evaluate the statistical significance of chromatin state assignments. Here, we propose the first method for assigning calibrated confidence scores to chromatin state annotations. Toward this goal, we performed a comprehensive evaluation of the reproducibility of the two most widely used existing SAGA methods, ChromHMM and Segway. We found that their predictions are frequently irreproducible. For example, when applying the same SAGA method on two sets of experimental replicates, 27%-69% of predicted enhancers fail to replicate. This suggests that a substantial fraction of predicted elements in existing chromatin state annotations cannot be relied upon. To remedy this problem, we introduce SAGAconf, a method for assigning a measure of confidence (r-value) to chromatin state annotations. SAGAconf works with any SAGA method and assigns an r-value to each genomic bin of a chromatin state annotation that represents the probability that the label of this bin will be reproduced in a replicated experiment. Thus, SAGAconf allows a researcher to select only the reliable predictions from a chromatin annotation for use in downstream analyses.

Fully automated landmarking and facial segmentation on 3D photographs.

Three-dimensional facial stereophotogrammetry provides a detailed representation of craniofacial soft tissue without the use of ionizing radiation. While manual annotation of landmarks serves as the current gold standard for cephalometric analysis, it is a time-consuming process and is prone to human error. The aim in this study was to develop and evaluate an automated cephalometric annotation method using a deep learning-based approach. Ten landmarks were manually annotated on 2897 3D facial photographs. The automated landmarking workflow involved two successive DiffusionNet models. The dataset was randomly divided into a training and test dataset. The precision of the workflow was evaluated by calculating the Euclidean distances between the automated and manual landmarks and compared to the intra-observer and inter-observer variability of manual annotation and a semi-automated landmarking method. The workflow was successful in 98.6% of all test cases. The deep learning-based landmarking method achieved precise and consistent landmark annotation. The mean precision of 1.69 ± 1.15 mm was comparable to the inter-observer variability (1.31 ± 0.91 mm) of manual annotation. Automated landmark annotation on 3D photographs was achieved with the DiffusionNet-based approach. The proposed method allows quantitative analysis of large datasets and may be used in diagnosis, follow-up, and virtual surgical planning.

Investigation on task effect analysis and optimization strategy of multimodal large model based on Transformers architecture for various languages

As artificial intelligence technology advances swiftly, the Transformers architecture has emerged as a pivotal model for handling multimodal data. This investigation delves into the impact of multimodal large-scale models utilizing the Transformers architecture for addressing various linguistic tasks, along with proposing optimization approaches tailored to this context. Through a series of experiments, this study scrutinized the performance of these models on multilingual datasets, engaging in a comprehensive analysis of the key determinants influencing their effectiveness. Firstly, several models of transformers architecture are pre trained on the same corpus, including ERNIE, GPT, ViT, VisualBERT, and a series of tests are carried out on these models in English, Chinese, Spanish and other languages. By comparing the performance of different models, it is found that these models show significant performance differences when dealing with tasks in different languages. Further, through analysis and experimental verification, this paper proposes a series of optimization strategies for different languages, including: annotation method for language specific datasets, incremental fine-tuning method for tuning, increasing the size of datasets, using multi task learning, etc. Experiments show that these methods have achieved remarkable results, and put forward the future research direction.

Semantic sensor data annotation method for industrial scene efficiency optimization to enable digital economy

AbstractIn the digital economy era, efficiently leveraging the vast amount of sensor data generated by the Industrial Internet of Things (IIoT) is essential. This paper presents an innovative semantic annotation method for industrial sensor data, designed to optimize data processing and enhance system efficiency. Our method combines cluster analysis, ontology development, and rule‐based reasoning to automatically annotate IIoT sensory data. By utilizing data aggregation and filtering mechanisms, which incorporate the DBSCAN (Density‐Based Spatial Clustering of Applications with Noise) algorithm and a rule engine, we significantly reduce the data volume required for annotation. The Semantic Web Rule Language aids in naming new concepts and properties identified through clustering, contributing further to the automation of data processing. Experimental results, using public datasets, validate the effectiveness of our method, showing a reduction in data volume by about 20% and underscoring its potential in enhancing industrial systems' automation and overall efficiency.

PCGIMA: developing the web server for human position-defined CpG islands methylation analysis.

Introduction: CpG island (CGI) methylation is one of the key epigenomic mechanisms for gene expression regulation and chromosomal integrity. However, classical CGI prediction methods are neither easy to locate those short and position-sensitive CGIs (CpG islets), nor investigate genetic and expression pattern for CGIs under different CpG position- and interval- sensitive parameters in a genome-wide perspective. Therefore, it is urgent for us to develop such a bioinformatic algorithm that not only can locate CpG islets, but also provide CGI methylation site annotation and functional analysis to investigate the regulatory mechanisms for CGI methylation. Methods: This study develops Human position-defined CGI prediction method to locate CpG islets using high performance computing, and then builds up a novel human genome annotation and analysis method to investigate the connections among CGI, gene expression and methylation. Finally, we integrate these functions into PCGIMA to provide relevant online computing and visualization service. Results: The main results include: (1) Human position-defined CGI prediction method is more efficient to predict position-defined CGIs with multiple consecutive (d) values and locate more potential short CGIs than previous CGI prediction methods. (2) Our annotation and analysis method not only can investigate the connections between position-defined CGI methylation and gene expression specificity from a genome-wide perspective, but also can analysis the potential association of position-defined CGIs with gene functions. (3) PCGIMA (http://www.combio-lezhang.online/pcgima/home.html) provides an easy-to-use analysis and visualization platform for human CGI prediction and methylation. Discussion: This study not only develops Human position-defined CGI prediction method to locate short and position-sensitive CGIs (CpG islets) using high performance computing to construct MR-CpGCluster algorithm, but also a novel human genome annotation and analysis method to investigate the connections among CGI, gene expression and methylation. Finally, we integrate them into PCGIMA for online computing and visualization.

Calibration-free structured-light-based 3D scanning system in laparoscope for robotic surgery.

Accurate 3D shape measurement is crucial for surgical support and alignment in robotic surgery systems. Stereo cameras in laparoscopes offer a potential solution; however, their accuracy in stereo image matching diminishes when the target image has few textures. Although stereo matching with deep learning has gained significant attention, supervised learning requires a large dataset of images with depth annotations, which are scarce for laparoscopes. Thus, there is a strong demand to explore alternative methods for depth reconstruction or annotation for laparoscopes. Active stereo techniques are a promising approach for achieving 3D reconstruction without textures. In this study, a 3D shape reconstruction method is proposed using an ultra-small patterned projector attached to a laparoscopic arm to address these issues. The pattern projector emits a structured light with a grid-like pattern that features node-wise modulation for positional encoding. To scan the target object, multiple images are taken while the projector is in motion, and the relative poses of the projector and a camera are auto-calibrated using a differential rendering technique. In the experiment, the proposed method is evaluated by performing 3D reconstruction using images obtained from a surgical robot and comparing the results with a ground-truth shape obtained from X-rayCT.

DVA: predicting the functional impact of single nucleotide missense variants

BackgroundIn the past decade, single nucleotide variants (SNVs) have been identified as having a significant relationship with the development and treatment of diseases. Among them, prioritizing missense variants for further functional impact investigation is an essential challenge in the study of common disease and cancer. Although several computational methods have been developed to predict the functional impacts of variants, the predictive ability of these methods is still insufficient in the Mendelian and cancer missense variants.ResultsWe present a novel prediction method called the disease-related variant annotation (DVA) method that predicts the effect of missense variants based on a comprehensive feature set of variants, notably, the allele frequency and protein–protein interaction network feature based on graph embedding. Benchmarked against datasets of single nucleotide missense variants, the DVA method outperforms the state-of-the-art methods by up to 0.473 in the area under receiver operating characteristic curve. The results demonstrate that the proposed method can accurately predict the functional impact of single nucleotide missense variants and substantially outperforms existing methods.ConclusionsDVA is an effective framework for identifying the functional impact of disease missense variants based on a comprehensive feature set. Based on different datasets, DVA shows its generalization ability and robustness, and it also provides innovative ideas for the study of the functional mechanism and impact of SNVs.

SOIRP: Subject-Object Interaction and Reasoning Path based joint relational triple extraction by table filling

Joint relational triple extraction methods based on table filling have gained considerable attention in recent years due to remarkable effectiveness and capabilities of extracting relational triples from complicated sentences. However, most of the existing methods disregard the impact of the information interaction between the subject and object in the relational triple and the intrinsic association among relational triples on relational overlapping triples extraction, resulting in the inability to extract the relational triples hidden in the sentence. Furthermore, previous sequence annotation methods failed to consider the case of entity nesting. Therefore, there is still room for improving the relational overlap problem and entity nesting problem. To address this issue, we propose a new joint relational triple extraction model based on table filling, SOIRP, which contains a Subject-Object Interaction (SOI) module consisting of several different interactions and a Reasoning Path Extraction (RPE) module based on the idea of decoder module in Transformer. We refine the subject-object interaction information captured by the former and the path inference information captured by the latter through multiple iterations. In addition, we introduce a new table filling method and decoding strategy, which can capture all entities in each sentence and align the boundary tokens of entity pairs for each relation. We evaluate our proposed model on two public datasets and the experimental results demonstrate that our model outperforms the baseline model. The code of our model can be available at: https://github.com/valentiner/SOIPR.

Towards clinically applicable automated mandibular canal segmentation on CBCT

ObjectivesTo develop a deep learning-based system for precise, robust, and fully automated segmentation of the mandibular canal on cone beam computed tomography (CBCT) images. MethodsThe system was developed on 536 CBCT scans (training set: 376, validation set: 80, testing set: 80) from one center and validated on an external dataset of 89 CBCT scans from 3 centers. Each scan was annotated using a multi-stage annotation method and refined by oral and maxillofacial radiologists. We proposed a three-step strategy for the mandibular canal segmentation: extraction of the region of interest based on 2D U-Net, global segmentation of the mandibular canal, and segmentation refinement based on 3D U-Net. ResultsThe system consistently achieved accurate mandibular canal segmentation in the internal set (Dice similarity coefficient [DSC], 0.952; intersection over union [IoU], 0.912; average symmetric surface distance [ASSD], 0.046 mm; 95% Hausdorff distance [HD95], 0.325 mm) and the external set (DSC, 0.960; IoU, 0.924; ASSD, 0.040 mm; HD95, 0.288 mm). ConclusionsThese results demonstrated the potential clinical application of this AI system in facilitating clinical workflows related to mandibular canal localization. Clinical significanceAccurate delineation of the mandibular canal on CBCT images is critical for implant placement, mandibular third molar extraction, and orthognathic surgery. This AI system enables accurate segmentation across different models, which could contribute to more efficient and precise dental automation systems.

Uncertainty-aware single-cell annotation with a hierarchical reject option.

Automatic cell type annotation methods assign cell type labels to new datasets by extracting relationships from a reference RNA-seq dataset. However, due to the limited resolution of gene expression features, there is always uncertainty present in the label assignment. To enhance the reliability and robustness of annotation, most machine learning methods address this uncertainty by providing a full reject option, i.e. when the predicted confidence score of a cell type label falls below a user-defined threshold, no label is assigned and no prediction is made. As a better alternative, some methods deploy hierarchical models and consider a so-called partial rejection by returning internal nodes of the hierarchy as label assignment. However, because a detailed experimental analysis of various rejection approaches is missing in the literature, there is currently no consensus on best practices. We evaluate three annotation approaches (i) full rejection, (ii) partial rejection, and (iii) no rejection for both flat and hierarchical probabilistic classifiers. Our findings indicate that hierarchical classifiers are superior when rejection is applied, with partial rejection being the preferred rejection approach, as it preserves a significant amount of label information. For optimal rejection implementation, the rejection threshold should be determined through careful examination of a method's rejection behavior. Without rejection, flat and hierarchical annotation perform equally well, as long as the cell type hierarchy accurately captures transcriptomic relationships. Code is freely available at https://github.com/Latheuni/Hierarchical_reject and https://doi.org/10.5281/zenodo.10697468.

A novel automatic annotation method for whole slide pathological images combined clustering and edge detection technique

AbstractPixel‐level labeling of regions of interest in an image is a key step in building a labeled training dataset for supervised deep learning networks of images. However, traditional manual labeling of cancerous regions in digital pathological images by doctors is time‐consuming and inefficient. To address this issue, this paper proposes an automatic labeling method for whole slide images, which combines clustering and edge detection techniques. The proposed method utilizes the multi‐level feature fusion model and the Long‐Short Term Memory network to discriminate the cancerous nature of the whole slide images, thereby improving the classification accuracy of the whole slide images. Subsequently, the automatic labeling of cancerous regions is achieved by integrating a density‐based clustering algorithm and an edge point extraction algorithm, both based on the discriminated results of the cancerous properties of whole slide images. The experimental results demonstrate the effectiveness of the proposed method, which offers an efficient and accurate solution to the challenging task of cancerous region labeling in digital pathological images.

AnnoSM: An Automated Annotation Tool for Determining the Substituent Modes on the Parent Skeleton Based on a Characteristic MS/MS Fragment Ion Library.

Mass spectrometry (MS) is a powerful technology for the structural elucidation of known or unknown small molecules. However, the accuracy of MS-based structure annotation is still limited due to the presence of numerous isomers in complex matrices. There are still challenges in automatically interpreting the fine structure of molecules, such as the types and positions of substituents (substituent modes, SMs) in the structure. In this study, we employed flavones, flavonols, and isoflavones as examples to develop an automated annotation method for identifying the SMs on the parent molecular skeleton based on a characteristic MS/MS fragment ion library. Importantly, user-friendly software AnnoSM was built for the convenience of researchers with limited computational backgrounds. It achieved 76.87% top-1 accuracy on the 148 authentic standards. Among them, 22 sets of flavonoid isomers were successfully differentiated. Moreover, the developed method was successfully applied to complex matrices. One such example is the extract of Ginkgo biloba L. (EGB), in which 331 possible flavonoids with SM candidates were annotated. Among them, 23 flavonoids were verified by authentic standards. The correct SMs of 13 flavonoids were ranked first on the candidate list. In the future, this software can also be extrapolated to other classes of compounds.

Annotating microbial functions with ProkFunFind.

Analyzing microbial genomes has become an essential part of microbiology research, giving valuable insights into the functions and evolution of microbial species. Identifying genes of interest and assigning putative annotations to those genes is a central task in genome analysis, and a plethora of tools and approaches have been developed for this task. The ProkFunFind tool was developed to bridge the gap between these various annotation approaches, providing a flexible and customizable search approach to annotate microbial functions. ProkFunFind is designed around hierarchical definitions of biological functions, where individual genes can be identified using heterogeneous search terms consisting of sequences, profile hidden Markov models, protein domains, and orthology groups. This flexible and customizable search approach allows for searches to be tailored to specific biological functions, and the search results are output in multiple formats to facilitate downstream analyses. The utility of the ProkFunFind search tool was demonstrated through its application in searching for bacterial flagella, which are complex organelles composed of multiple genes. Overall, ProkFunFind provides an accessible and flexible way to integrate multiple types of annotation and sequence data while annotating biological functions in microbial genomes.IMPORTANCEGenome sequencing and analysis are increasingly important parts of microbiology, providing a way to predict metabolic functions, identify virulence factors, and understand the evolution of microbes. The expanded use of genome sequencing has also brought an abundance of search and annotation methods, but integrating the information from these different methods can be challenging and is often done through ad hoc approaches. To bridge the gap between different types of annotations, we developed ProkFunFind, a flexible and customizable search tool incorporating multiple search approaches and annotation types to annotate microbial functions. We demonstrated the utility of ProkFunFind by searching for gene clusters encoding flagellar genes using a combination of different annotation types and searches. Overall, ProkFunFind provides a reproducible and flexible way to identify gene clusters of interest, facilitating the meaningful analysis of new and existing microbial genomes.

Deep learning contour-based method for semi-automatic annotation of manufactured objects in electron microscopy images

Deep learning contour-based method for semi-automatic annotation of manufactured objects in electron microscopy images

Accurate cell type annotation for single‐cell chromatin accessibility data via contrastive learning and reference guidance

AbstractRecent advances in single‐cell chromatin accessibility sequencing (scCAS) technologies have resulted in new insights into the characterization of epigenomic heterogeneity and have increased the need for automatic cell type annotation. However, existing automatic annotation methods for scCAS data fail to incorporate the reference data and neglect novel cell types, which only exist in a test set. Here, we propose RAINBOW, a reference‐guided automatic annotation method based on the contrastive learning framework, which is capable of effectively identifying novel cell types in a test set. By utilizing contrastive learning and incorporating reference data, RAINBOW can effectively characterize the heterogeneity of cell types, thereby facilitating more accurate annotation. With extensive experiments on multiple scCAS datasets, we show the advantages of RAINBOW over state‐of‐the‐art methods in known and novel cell type annotation. We also verify the effectiveness of incorporating reference data during the training process. In addition, we demonstrate the robustness of RAINBOW to data sparsity and number of cell types. Furthermore, RAINBOW provides superior performance in newly sequenced data and can reveal biological implication in downstream analyses. All the results demonstrate the superior performance of RAINBOW in cell type annotation for scCAS data. We anticipate that RAINBOW will offer essential guidance and great assistance in scCAS data analysis. The source codes are available at the GitHub website (BioX‐NKU/RAINBOW).