Background: Surat district and neighbouring districts like Valsad, Navsari in South Gujarat region are considered as an endemic area for leptosprirosis. Types of leptospira serovars are depend on geographical area so it was important to find serovars which are predominant in humans, cattle and environment (soil, water) of this area.So aim of the study was to establish the transmission cycle of leprospirosis in this area by Isolation techniques and serology. Methods: 207 human, 92 rodent and 258 animal blood samples were subjected to PCR, MAT test and culture in EMJH media. In addition, 550 animal urine, 7 rodent urine, 19 animal kidney and 571 rodent kidney were also tested by PCR. Results: Out of total 207 suspected cases, 161 samples were positive by MAT and 94(45%) positive by Real time PCR. 51(24%) samples were positive by MAT and PCR both tests. In cattle and rodent, 65 and 9 serum samples were positive by MAT test for leptospirosis.Predominant serovars observed in human cases, cattle and rodents were L. Autumnalis, L.Canicola, L. Pomona and L. Icterohaemorrahgiae. Conclusion: The current study reveals the presence of common leptospiral serovars, Autumnalis and Pomona infecting man, animals, and rodents, by serology and isolation. Hence this study reiterates the need for a strong and highly detailed control program for leptospirosis in this region, which should begin from the rodents and encompass the domestic animals as well. DOI: 10.21276/APALM.1282 Dear author(s), Congratulations ! Your article has been included and published in the current issue of Annals of Pathology and Laboratory Medicine. To view your article, kindly click on the following link: http://www.pacificejournals.com/journal/index.php/apalm/issue/view/apalm4.5 Please email us at editor.apalm@gmail.com if there is any correction i n your published article, within 3 working days from the receipt of the email. Any correction suggested after 3 days shall not be entertained. Please make the corrections’ list self-explanatory and easily understandable for a non-medical expert. If you have access to Acrobat, it may be helpful to mark the corrections in the PDF file using tools like PENCIL and NOTE. Or you can use following form for proofreading https://drive.google.com/open?id=0B4O8Udw-zRC_aEh6NXkxMXJ0MGM We encourage you to mention your article on your website and/or your social media profiles for its wider citation and referencing. We once again thank you for your intellectual contribution to APALM and look forward to have your long term association with us. Regards Co-Editor-in-Chief Annals of Pathology and Laboratory Medicine (APALM) www.pacificejournals.com/apalm www.apalm.org https://doi.org/10.21276/apalm Note: Any request for change in the article (excluding typographical errors or any unforeseen omissions on our part) shall be chargeable. DOI of your article will be active after 20 days of publication.