Australian Fauna Research Articles

‘Hook, line, and sinker’: Fluorescence in situ hybridisation (FISH) uncovers Trypanosoma noyesi in Australian questing ticks

Trypanosomes are blood-borne parasites infecting a range of mammalian hosts worldwide. In Australia, an increasing number of novel Trypanosoma species have been identified from various wildlife hosts, some of which are critically endangered. Trypanosoma noyesi is a recently described species of biosecurity concern, due to a close relationship to the South American human pathogen, Trypanosoma cruzi. This genetic similarity increases the risk for introduction of T. cruzi via a local vector. Unfortunately, there is a lack of knowledge concerning the vectorial capacity of Australian invertebrates for native Trypanosoma species. Australian ixodid ticks (Ixodidae), which are widespread ectoparasites of mammalian wildlife, have received the most attention as likely candidates for trypanosome transmission and have been previously implicated as vectors. However, as all studies to date have focused on blood-fed ticks collected directly from infected mammalian hosts, the question of whether ticks maintain a trypanosome infection between blood meals is unknown. In this study, we investigated the presence of Trypanosoma within 148 Australian adult and nymph questing ticks of the species Amblyomma triguttatum, Ixodes australiensis, Ixodes myrmecobii and larvae Ixodes spp., collected from an endemic region of south-west Australia. Using a novel HRM-qPCR detection method that can discriminate between species of Trypanosoma based on primer melting temperature (Tm), we report the first molecular detection of Trypanosoma DNA in Australian questing ticks, with 6 ticks DNA positive for T. noyesi. Additionally, the presence of intact T. noyesi parasites within all (n = 3) smeared gut and gland contents of questing ticks was confirmed using a fluorescence in situ hybridisation (FISH) assay. Whilst this study was unable to determine the in situ tissue location of trypanosomes for the purpose of discerning a potential route of transmission, these combined molecular and FISH smear data indicate that trypanosomes can persist in ticks between blood meals and that ticks are possibly vectors in the transmission of T. noyesi between native wildlife. Transmission experiments are still required to evaluate the competency of Australian ticks as vectors for T. noyesi. Nevertheless, these novel findings warrant further investigation concerning potential life stages and the development of trypanosomes in both Australian, and other, tick species.

Toxoplasma gondii seropositivity across urban and agricultural landscapes in an Australian owl

ABSTRACT In Australia, Toxoplasma gondii is an introduced parasite with a wide host range. House cats (Felis catus) are one of its definitive hosts. Little is known about T. gondii infection rates in Australian wildlife. Since cat abundance varies with landscape composition, we hypothesised that T. gondii infection would be more prevalent in urban and agricultural landscapes than intact bushland and sought to use samples from the Southern Boobook (Ninox boobook) as an indicator of ecosystem-wide T. gondii contamination. We used modified agglutination tests to determine T. gondii seropositivity in serum and meat juice samples taken from boobooks. Moderately low levels of seropositivity were detected and non-significant landscape-related patterns of seroprevalence were observed. We also examined correlations and interactions with owl age, season, injury status and exposure to an anticoagulant rodenticide. Only season showed significant correlations with observed seropositivity. To the best of our knowledge, this is the first published detection of T. gondii seropositivity in a wild predatory bird in Australia.

Meta-transcriptomic identification of Trypanosoma spp. in native wildlife species from Australia

BackgroundWildlife species carry a remarkable diversity of trypanosomes. The detection of trypanosome infection in native Australian fauna is central to understanding their diversity and host-parasite associations. The implementation of total RNA sequencing (meta-transcriptomics) in trypanosome surveillance and diagnosis provides a powerful methodological approach to better understand the host species distribution of this important group of parasites.MethodsWe implemented a meta-transcriptomic approach to detect trypanosomes in a variety of tissues (brain, liver, lung, skin, gonads) sampled from native Australian wildlife, comprising four marsupials (koala, Phascolarctos cinereus; southern brown bandicoot, Isoodon obesulus; swamp wallaby, Wallabia bicolor; bare-nosed wombat, Vombatus ursinus), one bird (regent honeyeater, Anthochaera phrygia) and one amphibian (eastern dwarf tree frog, Litoria fallax). Samples corresponded to both clinically healthy and diseased individuals. Sequencing reads were de novo assembled into contigs and annotated. The evolutionary relationships among the trypanosomatid sequences identified were determined through phylogenetic analysis of 18S rRNA sequences.ResultsWe detected trypanosome sequences in all six species of vertebrates sampled, with positive samples in multiple organs and tissues confirmed by PCR. Phylogenetic analysis indicated that the trypanosomes infecting marsupials were related to those previously detected in placental and marsupial mammals, while the trypanosome in the regent honeyeater grouped with avian trypanosomes. In contrast, we provide the first evidence for a trypanosome in the eastern dwarf tree frog that was phylogenetically distinct from those described in other amphibians.ConclusionsTo our knowledge, this is the first meta-transcriptomic analysis of trypanosomes in native Australian wildlife, expanding the known genetic diversity of these important parasites. We demonstrated that RNA sequencing is sufficiently sensitive to detect low numbers of Trypanosoma transcripts and from diverse hosts and tissues types, thereby representing an effective means to detect trypanosomes that are divergent in genome sequence.

Resolution of the identity of Pinnotheres latipes Hombron & Jacquinot, 1846 and description of a new species of Viridotheres Manning, 1996 (Decapoda: Brachyura: Pinnotheridae): two symbionts of bivalve molluscs

Abstract Pinnotherid crabs are well known as obligate associates of other marine invertebrates, frequently bivalve molluscs. Knowledge of the Australian pinnotherid crab fauna is expanded by description of a new species of Viridotheres Manning, 1996, representing the first record of the genus from Western Australia. The new species of Viridotheres is most similar to V. gracilis (Bürger, 1895) from the Philippines and V. takedai Ahyong, Komai & Watanabe, 2012 from Japan, but is unique in the genus in the elongated maxilliped 3 palp that distinctly overreaches the propodal apex. The identity of the poorly known northern Australian species, Pinnotheres latipes Hombron & Jacquinot, 1846, originally described from the Northern Territory, is resolved by designation of a neotype that places the species in the genus Nepinnotheres Manning, 1993. Nepinnotheres latipescomb. nov. is widespread in northern Australia and commonly hosted by the bivalve mollusc Pinna Linnaeus, 1758. Previous Australian records of Nepinnotheres cardii (Bürger, 1895) and N. edwardsi (De Man, 1888) from Australia are referable to N. latipes. Nepinnotheres latipes is one of only five known pinnotherids having androgynous males. Few pinnotherids are known from males, so estimating the extent of androgyny in Pinnotheridae must await discovery of males of additional species.

Review of the Stratiomyinae soldier flies of Australia (Diptera: Stratiomyidae), with a new genus and first records of Prosopochrysa de Meijere, 1907

A taxonomic treatment for the genera of the soldier fly subfamily Stratiomyinae is provided for the Australian fauna, including the first key to the Australian genera and updated key for the genus Acanthasargus White, 1914. Each genus is diagnosed and illustrated, including: Acanthasargus, Odontomyia Meigen, 1803, the newly recorded genus Prosopochrysa de Meijere, 1907 and new species Prosopochrysa lemannae Lessard & Woodley, sp.n., and the new genus and species Scutellumina parvatra Woodley & Lessard, gen.n. et. sp.n. Four genera and 30 species of Stratiomyinae are now recognised from Australia.

Impact of 2019-2020 mega-fires on Australian fauna habitat.

Australia's 2019-2020 mega-fires were exacerbated by drought, anthropogenic climate change and existing land-use management. Here, using a combination of remotely sensed data and species distribution models, we found these fires burnt ~97,000 km2 of vegetation across southern and eastern Australia, which is considered habitat for 832 species of native vertebrate fauna. Seventy taxa had a substantial proportion (>30%) of habitat impacted; 21 of these were already listed as threatened with extinction. To avoid further species declines, Australia must urgently reassess the extinction vulnerability of fire-impacted species and assist the recovery of populations in both burnt and unburnt areas. Population recovery requires multipronged strategies aimed at ameliorating current and fire-induced threats, including proactively protecting unburnt habitats.

Wicked “wild dogs”: Australian public awareness of and attitudes towards dingoes and dingo management

Public opposition has shaped management of wild animals in Australia, but public interest in dingo control has been minimal. We hypothesised that this is due to lack of awareness of dingo management practices, in part because using the term “wild dogs” to describe management renders “dingoes” invisible, framing the issue as one of control of introduced pests rather than control of an iconic Australian animal. We distributed an online questionnaire survey to the Australian public (N = 811) to measure how the public perceived dingoes and their management, how these views compared with other animals managed as pests in Australia, and whether the term “wild dogs” has shaped views and knowledge of dingo management. Most respondents (84.6%) considered dingoes to be native to Australia and there was low approval of lethal control methods, except when justification was provided (e.g., to protect livestock or endangered native species). Only 19.1% were aware that “wild dog” management included dingoes, and attitudes towards “wild dogs” were more negative than those towards dingoes. If public awareness about dingo management increases, pressure from the public may result and shape future management actions, including restricting the use of lethal control practices like poison baiting on public lands. As such, public attitudes should be incorporated into decision-making, and appropriate communication strategies need to be employed to prevent backlash.

After the Megafires: What Next for Australian Wildlife?

The 2019–2020 megafires in Australia brought a tragic loss of human life and the most dramatic loss of habitat for threatened species and devastation of ecological communities in postcolonial history. What must be done now to keep impacted species from extinction? What can be done to avoid a repeat of the impacts of such devastating bushfires? Here, we describe hard-won lessons that may also be of global relevance.

Serosurveillance and Molecular Investigation of Wild Deer in Australia Reveals Seroprevalence of Pestivirus Infection

Since deer were introduced into Australia in the mid-1800s, their wild populations have increased in size and distribution, posing a potential risk to the livestock industry, through their role in pathogen transmission cycles. In comparison to livestock, there are limited data on viral infections in all wildlife, including deer. The aim of this study was to assess blood samples from wild Australian deer for serological evidence of exposure to relevant viral livestock diseases. Blood samples collected across eastern Australia were tested by ELISA to detect antigens and antibodies against Pestivirus and antibodies against bovine herpesvirus 1. A subset of samples was also assessed by RT-PCR for Pestivirus, Simbu serogroup, epizootic hemorrhagic disease virus and bovine ephemeral fever virus. Our findings demonstrated a very low seroprevalence (3%) for ruminant Pestivirus, and none of the other viruses tested were detected. These results suggest that wild deer may currently be an incidental spill-over host (rather than a reservoir host) for Pestivirus. However, deer could be a future source of viral infections for domestic animals in Australia. Further investigations are needed to monitor pathogen activity and quantify possible future infectious disease impacts of wild deer on the Australian livestock industry.

Concentrations of some legacy pollutants have increased in South Australian bottlenose dolphins from 1989 to 2014

Information about pollution and its potential impact in Australian marine wildlife is scarce. To fill this knowledge gap, our study investigated concentrations of legacy pollutants as well as naturally produced methoxylated polybrominated diphenyl ethers (MeO-PBDEs) in blubber, liver, kidney and muscle of Indo-Pacific bottlenose dolphins (Tursiops aduncus) from two large inverse estuaries in South Australia from 1989 to 1995 and 2009–2014. Our results show that concentrations of most pollutant classes are relatively low compared to the literature but at the higher end of the ranges reported for marine mammals in Australia. Results for some individuals exceed toxicity thresholds indicative of immunotoxicity in marine mammals. It is important to note that concentrations of some compound classes, particularly PBDEs and polychlorinated biphenyls (PCBs), increased over a time interval of 20 years thereby placing more individuals at risk in recent years. Some of the highest concentrations of persistent organic pollutants (POPs) were measured in juveniles, which may jeopardize their development and the success of future generations. These results indicate that legacy pollutants may play a role in the long-term health of T. aduncus and should be included in biomonitoring efforts.

Novel hepaci- and pegi-like viruses in native Australian wildlife and non-human primates.

The Flaviviridae family of positive-sense RNA viruses contains important pathogens of humans and other animals, including Zika virus, dengue virus, and hepatitis C virus. The Flaviviridae are currently divided into four genera—Hepacivirus, Pegivirus, Pestivirus, and Flavivirus—each with a diverse host range. Members of the genus Hepacivirus are associated with an array of animal species, including humans, non-human primates, other mammalian species, as well as birds and fish, while the closely related pegiviruses have been identified in a variety of mammalian taxa, also including humans. Using a combination of total RNA and whole-genome sequencing we identified four novel hepaci-like viruses and one novel variant of a known hepacivirus in five species of Australian wildlife. The hosts infected comprised native Australian marsupials and birds, as well as a native gecko (Gehyra lauta). From these data we identified a distinct marsupial clade of hepaci-like viruses that also included an engorged Ixodes holocyclus tick collected while feeding on Australian long-nosed bandicoots (Perameles nasuta). Distinct lineages of hepaci-like viruses associated with geckos and birds were also identified. By mining the SRA database we similarly identified three new hepaci-like viruses from avian and primate hosts, as well as two novel pegi-like viruses associated with primates. The phylogenetic history of the hepaci- and pegi-like viruses as a whole, combined with co-phylogenetic analysis, provided support for virus-host co-divergence over the course of vertebrate evolution, although with frequent cross-species virus transmission. Overall, our work highlights the diversity of the Hepacivirus and Pegivirus genera as well as the uncertain phylogenetic distinction between.

The youngest Ordovician (latest Katian) coral fauna from eastern Australia, in the uppermost Malachis Hill Formation of central New South Wales

Corals representing the sole occurrence of C/S Fauna IV of the regional biostratigraphic scheme occur in limestone in the uppermost Malachis Hill Formation of the Bowan Park area, central New South Wales. New species described and illustrated from this fauna include the rugosan Bowanophyllum ramosum and tabulate Hemiagetiolites longiseptatus. Also documented are three other distinct species of Hemiagetiolites, H. spinimarginatus (Hall, 1975), H. sp. cf. H. palaeofavositoides (Lin & Chow, 1977) and H. sp., an unnamed species of Paleofavosites, and three heliolitine corals, Heliolites waicunensis Lin & Chow, 1977, Navoites cargoensis (Hill, 1957) and Plasmoporella sp. These descriptions, which complete knowledge of the entire fauna collected over 50 years, enable interesting conclusions to be drawn regarding palaeogeographic affinities of this youngest in situ Ordovician coral fauna known from eastern Australia. Its remarkable similarities to contemporaneous coral faunas from South Tien Shan and the Chu-Ili Terrane indicate strong connections between eastern Australia and the latter two terranes, supporting their positioning in equatorial latitudes of eastern peri-Gondwana. However, it is puzzling that fewer similarities exist between the eastern Australian coral fauna and those from rocks of Katian age in SE China, which may be due to either a slightly younger age of the former fauna or a relatively higher palaeolatitudinal position of South China.Guangxu Wang [gxwang@nigpas.ac.cn], State Key Laboratory of Palaeobiology and Stratigraphy; Center for Excellence in Life and Paleoenvironment, Chinese Academy of Sciences, Nanjing Institute of Geology and Palaeontology, Nanjing 210008, China; Ian G. Percival [ian.percival@planning.nsw.gov.au], and Yong Yi Zhen [yong-yi.zhen@planning.nsw.gov.au], Geological Survey of New South Wales, 947–953 Londonderry Road, Londonderry, NSW 2753, Australia.

<p class="ZootaxaTitle"><strong>Curious Caddis Couture: Form and function among cases of Australian Hydroptilidae</strong></p>

Trichoptera larvae that construct portable cases occur worldwide, in some groups building highly distinctive cases. Fifth instar larvae of several genera in the micro-caddisfly family Hydroptilidae always build cases of the same form, thus affording ready identification of their larvae and pupae to genus level. Examples are Oxyethira and Orthotrichia: the former have transparent flask-shaped silk (secretion) cases, the latter ‘wheat seed’-shaped silk cases that are generally dark brown to black in colour. Additionally, in the fauna of mainland Australia, cases of the endemic genus Orphninotrichia are unmistakable in form; enigmatically, however, quite different forms are seen in two of the four locally endemic species on the small, off-shore, oceanic island of Lord Howe. The larval cases of some other Australian genera also vary considerably, some in materials (e.g., Hydroptila) and others in both materials and shape (e.g., Hellyethira and an Australian endemic genus, Maydenoptila). Known larvae of microcaddisfly species in the Australian fauna are examined in search of patterns in the three most obviously variable attributes of cases: mode of construction, shape, and materials. Possible relationships between form and function are postulated for some of the cases.

A Divergent Articulavirus in an Australian Gecko Identified Using Meta-Transcriptomics and Protein Structure Comparisons.

The discovery of highly divergent RNA viruses is compromised by their limited sequence similarity to known viruses. Evolutionary information obtained from protein structural modelling offers a powerful approach to detect distantly related viruses based on the conservation of tertiary structures in key proteins such as the RNA-dependent RNA polymerase (RdRp). We utilised a template-based approach for protein structure prediction from amino acid sequences to identify distant evolutionary relationships among viruses detected in meta-transcriptomic sequencing data from Australian wildlife. The best predicted protein structural model was compared with the results of similarity searches against protein databases. Using this combination of meta-transcriptomics and protein structure prediction we identified the RdRp (PB1) gene segment of a divergent negative-sense RNA virus, denoted Lauta virus (LTAV), in a native Australian gecko (Gehyra lauta). The presence of this virus was confirmed by PCR and Sanger sequencing. Phylogenetic analysis revealed that Lauta virus likely represents a newly described genus within the family Amnoonviridae, order Articulavirales, that is most closely related to the fish virus Tilapia tilapinevirus (TiLV). These findings provide important insights into the evolution of negative-sense RNA viruses and structural conservation of the viral replicase among members of the order Articulavirales.

Dung beetles of an Australian tropical savanna: Species composition, food preferences and responses to experimental fire regimes

AbstractDung beetles are an ecologically important group of insects globally, but the ecology of the Australian fauna is poorly known. Here, we report on the first ecological study of dung beetles in an Australian tropical savanna, documenting species composition, food preferences and responses to fire. Dung beetles were baited using dung from five types of vertebrates as well as mushrooms. We sampled at nine plots subject to experimental fire regimes (three replicates each of high fire severity, moderate fire severity and unburnt over 15 years) at the Territory Wildlife Park near Darwin. Our samples were comprised entirely of nine native species of Onthophagus. Dog dung was the most attractive bait, recording all nine species and nearly 80% of all individuals, whereas no beetles were recorded at either wallaby or magpie‐goose dung. Dung beetle abundance was highest under high fire severity and was negatively correlated with the cover of woody vegetation, a result driven by the two most common species, O. minsiculus and O. nr. quadripunctulatus. Our results indicate that Onthophagus is the dominant genus in dung‐beetle assemblages of monsoonal Australia, as it is in open habitats of the Australian wet tropics. All our species of Onthophagus are relatively small (≤10 mm body length) and are ‘tunnellers’, storing dung and other food resources in tunnels dug immediately below the food source. Such an overwhelming dominance by small tunnellers does not occur in African savannas, reflecting the far lower availability of large mammalian dung in Australia.

Relaxed predation theory: size, sex and brains matter.

Australia's wildlife is being considerably impacted by introduced mammalian predators such as cats (Felis catus), dogs (Canis lupus familiaris), and foxes (Vulpes vulpes). This is often attributed to native wildlife being naïve to these introduced predators. A systematic review of the literature reveals that native metatherians (body mass range 0.02-25 kg) do not recognise, and show relaxed antipredator behaviours towards, native and some introduced mammalian predators. Native eutherians (all with body mass < 2 kg), however, do appear to recognise and exhibit antipredator behaviours towards both native and introduced predators. Based on our findings, we propose a novel theory, the 'Relaxed Predation Theory'. Our new theory is based on the absence of large mammalian predators leading to reduced predation pressure in Australia during the past 40000-50000 years, and on three key differences between Australian metatherians and eutherians: size, sex, and brains. In light of this Relaxed Predation Theory, we make a number of recommendations for the conservation of Australian wildlife: (i) predator avoidance training of suitable species; (ii) exclusion fencing to exclude some, but not all, predators to facilitate the development of antipredator behaviours; (iii) captive breeding programs to prevent the extinction of some species; and (iv) reintroduction of Australia's larger predators, potentially to compete with and displace introduced predators. A more detailed understanding of the responses of Australian mammals to predators will hopefully contribute to the improved conservation of susceptible species.

Exploring nationality and social identity to explain attitudes toward conservation actions in the United States and Australia.

Understanding human attitudes toward wildlife management is critical to implementing effective conservation action and policy. Understanding the factors that shape public attitudes toward different wildlife management actions is limited, however, which can result in unpredictable public responses to interventions. We drew on comparisons between residents of 2 countries on separate continents to explore differences in attitudes toward wildlife management and determine factors important in shaping these attitudes. We surveyed representative publics via market research panels in Australia (n = 881 respondents) and the United States (n = 1287). We applied a social-identity approach and demography to identify factors that explained variance between responses about wildlife management. We compared responses between countries overall and within subgroups of respondents who strongly identified as environmentalists, animal rights activists, wildlife conservation advocates, and farmers. We created aggregate scores for the management-related response items per respondent and used regression analyses to identify the relative importance of country, identity, age, and gender in explaining variance between responses. These factors accounted for 15.3% of variance among responses. Australians overall were generally more accepting of lethal wildlife management actions than U.S. respondents. Differences in national attitudes reflected differences between United States and Australian wildlife management and policy, highlighting the importance of understanding social attitudes in shaping conservation policy. Identifying as a farmer followed by identifying as an animal rights activist most shaped attitudes toward wildlife management. Identity-related conflicts could be initiated or exacerbated by conservation interventions that fail to consider identity-related processes.

New Lobellini (Collembola: Neanuridae) from Queensland contribute to understanding distribution and ecology of Australian fauna

AbstractTwo new species from Queensland rainforest belonging to genera Hemilobella and Sphaeronura are described and illustrated with drawings and colour photographs. Hemilobella matildae sp. nov. Smolis and Greenslade is characterised by elongated tubercles laterally on body and an elongated ogival labrum. Sphaeronura ameliae sp. nov. Smolis and Greenslade differs from other taxa in the genus in chaetotaxic features and the presence of a male ventral organ. A new record of Australonura scoparia is included. The high species richness of lowland rainforests, in which these log inhabiting saproxylic species were found, is noted, and the contribution that extreme events such as cyclones in maintaining the fallen timber habitat is emphasised. The bright colour of these species may be caused by sequestered pigments from the slime moulds on which they feed. The colour probably also acts as a warning to predators that individuals are distasteful. A description of the distributions, characteristics, distinctiveness and possible genesis of Australian fauna of the subfamily Neanurinae is presented, and a checklist of species and key to all Australian genera of the subfamily is included. Species in this subfamily are predominately short‐range endemics so should be surveyed whenever wet forests are under threat from logging or urban expansion.

Understanding the human dimensions of managing overabundant charismatic wildlife in Australia

Management of charismatic, locally overabundant, wildlife such as koalas (Phascolarctos cinereus) and kangaroos (Macropus spp.) has led to public outcry on multiple occasions. While this public opinion has been influencing decision-making, evidence on what the general public thinks to support this decision-making has been largely lacking. Here we quantified the opinions (n = 1,148) of a representative sample of the Australian public on the acceptability of a range of management options for managing overabundant populations of native koalas, kangaroos and the introduced feral horses (Equus caballus). Our survey also measured environmental value orientations (introducing a new ‘animalistic’ dimension to the widely used biospheric-altruistic-egoistic orientations scale) and beliefs about human- wildlife relationships to determine drivers of acceptability. Lastly, we examined the effect of providing ecological information on acceptability by randomly selecting half the koala respondents to receive additional information on the impacts of koala overabundance. We found significant differences in the acceptability of different management options within and across species. While the public understood the need for intervention, lethal control options such as culling were generally considered unacceptable in koalas, but acceptable in kangaroos. The acceptability of culling koalas increased with the provision of information, but remained unacceptable overall. Both values and beliefs were useful in explaining the acceptability of different management options. Empirical evidence of public opinion such as that presented here will aid decision-makers in engaging the public and reduce conflict by revealing the basis of opinion in people's values and beliefs, leading to better management decisions and conservation outcomes.

A review of Australia’s Mesozoic fishes

The Australian Mesozoic fish fauna is considered to be depauperate in comparison with fish faunas in the Northern Hemisphere. However, due to its geographical location as a potential radiation center in the Southern Hemisphere, Australia’s Mesozoic fish fauna is important for understanding fish radiations. Most of the modern fish groups originated during the Mesozoic, but the first records of a modern fish fauna (freshwater and marine) in Australia does not occur until the lower Paleogene. Here, we review all known fossil fish-bearing localities from the Mesozoic of Australia, to improve the understanding of the record. The apparent low Australian Mesozoic fish diversity is likely due to its understudied status of the constituent fossils rather than to a depauperate record. In addition, we review recent work with the aim of placing the Australian Mesozoic fish fauna in a global context. We review the taxonomy of Australian fossil fishes and conclude that the assignments of many actinopterygians need major revision within a modern phylogenetic context. The vast majority of chondrichthyans are yet to be formally described; to the contrary all of the known lungfish specimens have been described. This study considers the microscopic and fragmented remains of Mesozoic fish already found in Australia, allowing a more complete view of the diversity of the fishes that once inhabited this continent.Rodney W. Berrell [r.berrell@postgrad.curtin.edu.au], School of Earth and Planetary Sciences, Curtin University, Kent Street, Bentley, Western Australia, 6102, Australia; Catherine Boisvert [Catherine.Boisvert@curtin.edu.au], School of Molecular and Life Sciences (MLS), Curtin University, Kent Street, Bentley, Western Australia, 6102, Australia; Kate Trinajstic [K.Trinajstic@curtin.edu.au], School of Molecular and Life Sciences (MLS), Curtin University, Kent Street, Bentley, Western Australia, 6102, Australia; Mikael Siversson# [mikael.siversson@museum.wa.gov.au], Department of Earth and Planetary Sciences, Western Australian Museum, 49 Kew Street, Welshpool, Western Australia, 6106, Australia; Jesús Alvarado-Ortega [jalvarado@yahoo.com.mx], Instituto Geologia Cd universitaria, Circuito de la investigacion, Del. coyoacan, C.P. 04510, Ciudad de México, México; Lionel Cavin [lionel.cavin@ville-ge.ch], Section of Earth Sciences, Muséum d’Histoire naturelle de la Ville de Genèeve, CP 6434, 1211 Genève 6, Switzerland; Steven W. Salisbury [s.salisbury@uq.edu.au], School of Biological Sciences, The University of Queensland, Brisbane St Lucia, Queensland, 4072, Australia; Anne Kemp [annerkemp@gmail.com], 9 Hampton Grove, Norwood, Adelaide, South Australia 5067, Australia. #Also affiliated with: School of Molecular and Life Sciences, Curtin University, Kent Street, Bentley, WA 6102, Australia.