Animal Products Research Articles

Effectiveness, Challenges, and Environmental Impacts of New Food Strategies with Plant and Animal Protein Products.

Sustainable food practices are intrinsically linked to human nutrition in the preservation of the ecosystem. This study, therefore, evaluates the effectiveness, challenges, environmental impacts, and new food strategies related to plant and animal products, with a view to promoting more sustainable and healthy eating practices. The search stages were conducted using the following databases: PubMed, Science Direct, and SciElo. The studies selected included those published from 2018 to 2024 and government documents, available in English, Portuguese, and Spanish. The 34 articles analyzed in this study showed the environmental impacts related to the production of plant and animal proteins, highlighting the urgency of implementing changes in this sector. However, factors such as land use, carbon footprint, and water footprint show remarkable differences depending on the type of crop cultivated, agricultural practices adopted, and stages involved in the supply chain. As final considerations, the analysis suggests that achieving sustainability in food systems requires an integrate approach that combines the optimization of plant protein production with a reduction in environmental impacts and the development of technologies that that support the efficiency and resilience of the industry. Meeting the nutritional needs of the population in a sustainable way will only be possible through regional actions and a deep understanding of the challenges and opportunities.

Revitalising a fading oral culture through innovative animation production: an experiment with Bodo oral narratives

PurposeThe paper aims to explore animation as a medium to revive the oral narrative of the Bodo community. The Bodo oral narratives are crucial for preserving the tribe's traditional values, but modernization is endangering their oral tradition. Transmitting folk culture to youth is crucial for its continuity and knowledge retention. The objective of the study was to examine the use of animation design approach for the transmission of oral narratives to the young generation.Design/methodology/approachThis design exploration involves a case study, field visit, user study, expert interaction for authentication and questionnaire-based survey. The approach integrates the animation design process with a popular myth of the Bodo tribe's musical instrument, Serja. This exploration seeks to visually present a reliable version of the story based on expert advice and published articles using instructional animation technique.FindingsThe positive response in the user survey suggests that the animation design exploration effectively represented the traditional and cultural aspects of the Bodo community. Feedback and comments from the participants have confirmed that animation can enhance the storytelling experience by effectively communicating oral stories.Research limitations/implicationsThe exploration in this research tries to establish a visual representation of the oral narratives of the Bodo community for the younger generation.Practical implicationsThe exploration in this research tries to establish a visual representation of the oral narratives of the Bodo community for the younger generation.Social implicationsBy using the digital medium, indigenous communities can sustain a long-lasting legacy of their oral stories.Originality/valueThe paper acknowledges the authentic essence of Bodo culture, rooted in their oral stories. Furthermore, it effectively captured and conveyed this essence using an animation design approach.

Determinants of nutrition security status of women in rural households in Northwest Ethiopia

The nutrition security of women is critically important for society’s well-being. Therefore, this study was conducted to determine the determinants of the nutrition security status of rural women in Northwest Ethiopia. Data collected from rural women in 197 randomly selected households were analyzed using a binary logit regression model. The results show that 72.6% of the women are nutritionally insecure. The determinants that significantly affect nutrition security status are family size (a negative effect), women’s daily feed frequency, milk consumption, feeding habits of fruits and vegetables, feeding habits of animal products, and women empowerment (all positive effects). The weight, height, and BMI of nutritionally insecure women were significantly lower than those of nutritionally secure women. These findings reveal the need for government and other stakeholders’ interventions to increase access to nutritious food products and to provide training on feeding culture and dietary diversity to women. The findings of this study can help the Government of Ethiopia to focus on the significant determinants to achieve its National Development Priorities focusing on the Sustainable Development Goals (SDGs) of the UN, particularly Goals 2, 3, and 5.

Precision livestock farming in the 21st century: Challenges and opportunities for sustainable agriculture

Abstract This study aimed to review the recent development of different technologies in precision livestock farming (PLF), along with their scopes and challenges. PLF is an innovative, contemporary, and fast-expanding approach to agriculture that aims to improve sustainable livestock farming. The growing global population has increased the demand for animal products. To meet this demand, farmers have to increase their production, so without the integration of precision technology, this cannot be achieved. PLF currently employs a variety of technologies. Some of these methods include vision-based solutions, load cells, accelerometers, microphones, thermal cameras, photoelectric sensors, and radio-frequency identification (RFID). Despite the availability of different PLF technologies, their adoption by farmers varies widely on the basis of the cost of investment, ease of operation, availability, and accessibility. These technologies are used to track different activities of livestock farming, such as feeding, drinking, physical behavior, temperature regulation, tracking and identification, estrus detection, disease detection, and milking. PLF contributes significantly to technological advancement, human-animal relationships, environmental sustainability, and increased productivity. It does, however, present a number of obstacles and eventual advantages.

Prioritization of chemical food safety hazards in the European feed supply chain.

Extensive monitoring programs of chemical hazards in the animal feed chain are in place, both organized by public and private organizations. The objective of this review was to prioritize chemical hazards for monitoring in the European animal feed supply chain. A step-wise approach was designed for the prioritization, based on: historical occurrence of the chemicals in animal feed ingredients and animal feeds (in relation to European guidance values or maximum limits in feed); information on transfer of the chemical to edible animal products, and; the extent of human dietary intake of the products and possible adverse human health effects of the chemical. Possible prioritization outcomes were: high (H), medium (M), or low (L) priority for monitoring, or classification not possible (NC) because of limited available data on the transfer of the chemical to edible animal tissues. The selection of chemicals included (with results in parentheses): dioxins and polychlorinated biphenyls (H); brominated flame retardants (H); per- and polyfluorinated alkyl substances (H); the heavy metals arsenic (H) and cadmium (H) as well as lead (M) and mercury (M); aflatoxins (H), ochratoxin A (NC), and other mycotoxins (L); pyrrolizidine alkaloids (H) and other plant toxins (NC); organochlorine pesticides (H) and other pesticides (L); pharmaceutically active substances (M); hormones (NC); polycyclic aromatic hydrocarbons (L), heat-induced processing contaminants (NC), and mineral oils (NC). Results of this study can be used to support risk-based monitoring by food safety authorities and feed-producing companies in Europe.

Assessing research needs for informing pet food industry decisions

Abstract The global pet food industry continues to grow being fueled by increased pet ownership, higher disposable income of pet owners, and a growing demand for premium products. While this growth has attracted increased interest of researchers in animal nutrition and product formulation, the overall body of management and economic literature to guide product innovation and marketing strategies in the pet food industry is still limited. This study aims to identify and highlight the most important, relevant, and current research needs for informing product and marketing decisions in pet food. The methods include a quantitative analysis of primary data from a survey of 76 pet food decision makers involved in the procurement of raw material, production, and marketing. The findings suggest the need for extensive research in various areas pertaining to the quality and availability of raw materials, processing methods, and forecasting consumer preferences. The findings also emphasize high value research areas such as identifying alternative protein sources, enhancing nutrient retention during the extrusion process, identifying opportunities for product innovation that align with customer preferences, and accurately predicting consumer demand.

Antimicrobial resistance: A significant public health issue of both human and veterinary concern

Abstract Introduction The discovery of antibiotics and the subsequent realization that these medications are the most effective treatments for a wide range of illnesses affecting the health of humans and animals constituted a revolution in medical history. Methods A review of the literature was done, looking for pertinent studies, using an explorative review method to discuss how the widespread use of antibiotics often led to bacterial resistance and antibiotic residue in food animals, which negatively impacted humans and animals. Results The study showed that treating bacterial resistance is a therapeutic challenge for physicians and veterinary professionals. Increased treatment failure rates, the severity of disease-causing infections, the frequency of blood infections, the length of time patients were sick, and the death rate in both humans and animals are all signs that the overuse of antibiotics is becoming a catastrophe. Discussion To prevent the negative effects of antibiotics in people, animals, and the environment, especially in developing nations, this article ends by recommending that strict guidelines, laws, and regulations regarding the use of antibiotics in clinical human and veterinary medicine be implemented. Concern must be expressed about the effects of inactivity on public health, both now and in the future. We may attempt to reverse the global resistance issue by increasing the use of antibiotics and reducing the frequency of resistance genes at all levels. Ultimately, the safety of animal products depends on the proper use of antibiotics in animal husbandry under the supervision of a veterinarian.

Using scenario tree modelling to evaluate the probability of freedom from Enzootic bovine leukosis (EBL) in Italy and Slovenia.

Documented freedom from disease is paramount for international free trade of animals and animal products. This study describes a scenario tree analysis to estimate the probability of freedom from Enzootic bovine leukosis (EBL) in Italy and Slovenia using information gathered via the data collection tool developed in the COST action project SOUND-control. Data on EBL control programmes (CPs) from 2018 to 2021 were used to build the models. Since animals are only sampled on the farm, one surveillance system component (SSC) was considered. The posterior probability of freedom (PostPfree) was estimated in time steps of one year, from 2018 to 2021. After each year, the calculated from the previous year, combined with the probability of introduction, was used as a prior probability for the next year. The herd level design prevalence was set to 0.2% in accordance with the Council Directive 64/432/EEC and the within herd design prevalence was set to 15%. As Slovenia implemented a risk-based surveillance, targeting the herds importing cattle, in its model the design herd prevalence was combined with an average adjusted risk to calculate the effective probability of a herd importing cattle being infected. The models were run for 10,000 iterations. Over the study period the mean estimates were: i) for Italy both the surveillance system sensitivity ( SSe) and PostPFree 100%, with no differences between simulations and years, ii) for Slovenia the SSe was 50.5% while the PostPFreewas 81.6%.

Methodology for comparative evaluation of high-protein feed for farm animals and poultry

The peculiarity of the proposed methodology is the inclusion in the evaluation criteria of indicators that characterise the biological value of protein (content of protein convertible into animal products, non-degradable protein) and its cost. Approbation of the methodology made it possible to build a rating of the most preferable forages for each type of farm animals and poultry both from the position of satisfying their physiological needs in nutrients and taking into account the need to reduce production costs.

Risk Propensity and Acceptance of Gene-edited and Genetically Modified Food among US Consumers: A Comparison between Plants and Animal Products

Abstract Utilizing online survey data of US consumers, this study examines the extent to which consumers' acceptance of genetically modified (GM) and gene-edited (GE) food is driven by their risk attitudes. Our results indicate that individuals with high-risk propensity are more likely to accept both GM and GE food than individuals with low- and medium-risk propensity. Our results also find differences in consumers' attitudes toward plants and animal products in the context of both GM and GE. Intriguingly, these attitudinal differences can be explained by consumers' risk propensities. Specifically, both low- and medium-risk propensity consumers differentiate between plants and animal products; the latter is less acceptable than the former, indicating a tendency to have more concerns about the application of biotechnology to animals than plants. However, individuals with high-risk propensity do not differentiate between GM and GE plants and animal products. Our results suggest that policymakers, the food industry, and researchers need to consider these attitudinal differences while studying consumer attitudes toward GM and GE food. Failing to capture these attitudinal differences in studies focusing on consumer behavior toward GM and GE food may result in either overestimating or underestimating consumer response.

Sustainable ferritin from bovine by-product liver as a potential resource: Ultrasound assisted extraction and physicochemical, structural, functional, and stable analysis

Ferritin is an iron-containing protein that is widely present in all organisms and has the function of regulating the metabolic balance of iron in organisms. It can be separated from animal by-product tissues and converted into value-added components to promote the development of a circular economy. This study established a method for extracting bovine liver ferritin (BLFer) using ultrasound-assisted buffer system. The optimal extraction conditions were determined through single factor and response surface optimization experiments as follows: ultrasound temperature of 70 °C, extraction time of 25 min, ultrasonic power of 400 W and solvent-to-solid ratio of 2 mL/g, the experimental value of the ferritin yield was 32.18 ± 0.12 g/kg. Compared with traditional methods, Ultrasound-assisted extraction improved the ferritin yield and enhanced its structural stability. Bioinformatics analysis revealed that BLFer is a hydrophilic protein with strong thermal stability. The BLFer has a ferrous oxidase active center, which plays an important role in the oxidative precipitation and reductive release of iron. It can dissociate under strong acids or bases but maintains good stability after heat treatment. These findings will help improve the comprehensive utilization of animal by-products, and promote the potential application of animal by-product ferritin in food and other industries.

Unveiling the Nutritional Quality of Terrestrial Animal Source Foods by Species and Characteristics of Livestock Systems.

Background. It is well-established that a range of macronutrients, micronutrients and bioactive compounds found in animal-source foods play unique and important roles in human health as part of a healthy diet. Methods. This narrative review focuses on terrestrial animal source foods (TASFs). It particularly analyzes five groups: poultry eggs, milk, unprocessed meat, foods from hunting and wildlife farming, and insects. The objectives were as follows: (1) examine the nutrient composition of TASFs within and across livestock species, drawing on the country and regional food composition databases; (2) analyze the influence of intrinsic animal characteristics and production practices on TASF nutritional quality. Results. TASFs are rich in high-quality proteins and fats, as well as micronutrients such as vitamin B12, iron or zinc. This study found differences in the nutritional quality of TASFs by livestock species and animal products, as well as by characteristics of livestock production systems. Our findings suggest that there may be public health opportunities by diversifying TASF consumption across species and improving certain aspects of the production systems to provide products that are both more sustainable and of higher quality. Conclusions. Future research should adopt a more holistic approach to examining the food matrix and the dietary patterns that influence TASF digestibility. It is necessary to include meat from hunting and wildlife farming and insects in global food composition databases, as limited literature was found. In addition, scarce research focuses on low- and middle-income countries, highlighting the need for further exploration of TASF food composition analysis and how intrinsic animal characteristics and livestock production system characteristics impact their nutritional value.

Crisis preparedness exercise on food and feed safety for IPA beneficiaries 2024.

The European Food Safety Authority (EFSA) within the framework of the Preparatory measures for the participation of IPA beneficiaries in the European Food Safety Authority 2023-2026 will organize a total of 3 crisis preparedness training events for the IPA countries (Albania, Bosnia and Herzegovina, Kosovo,* Montenegro, North Macedonia, Serbia, Türkiye). For 2024, EFSA requested to organise a simulation exercise focused on a multi-country incident related to biological risks in products of animal origin. The overall objectives of the training were: 1) to increase IPA countries' knowledge and understanding of crisis handling concepts and of EFSA's crisis handling procedures; 2) to improve preparedness and response planning for crisis situations in the domain of biological hazards; 3) to improve coherence, interoperability and coordination, to be prepared for communication and decision-making challenges in crisis situations. Working with the contractor Opera srl, EFSA organised a 2-day training on 26-27 June 2024 in Sarajevo (Bosnia and Herzegovina), attended by 24 participants (3 online) from 7 IPA countries, 2 representatives from EFSA and 1 representative from the hosting competent authority from Bosnia and Herzegovina. Participants were briefed on the IPA crisis preparedness training scheme, EFSA's crisis handling procedures, principles of crisis communication (remote mode), EFSA - ECDC Rapid Outbreak Assessment (remote mode), food safety risk assessment structures in Bosnia and Herzegovina and Serbia. Most of the workshop was dedicated to the simulation exercise, that was organized in a tabletop format, with injects describing an evolving food safety incident and a debriefing after each inject. During the exercise, 1 audio-conference meeting and a TV debate were simulated. The exercise was concluded with a brainstorming session between the tutors and participants. Three recommendations were done to improve the organization of future events. Six recommendations were given for the improvement of risk assessment, risk management and risk communication in IPA countries. The objectives were achieved, based on the results of the knowledge test and on the feedback provided by participants in the course evaluation questionnaire.

Effect of ultrasound and enzymatic pre-treatments on the profile of bioactive peptides of beef liver hydrolysates

The use of meat by-products as a potential source of protein hydrolysates rich in bioactive peptides is of growing interest to valorise these products and improve their sustainable use. This study aimed to evaluate the effect of different pre-treatments (hydrolysis with pepsin, ultrasounds, and ultrasounds-assisted pepsin hydrolysis) on the peptide profile and bioactivity of beef liver hydrolysed with flavourzyme. The pre-treatments with pepsin increased the degree of hydrolysis and generation of free amino acids in the liver hydrolysates, whereas the subsequent hydrolysis with flavourzyme influenced the molecular weight distribution of the peptides. Samples pre-treated with pepsin and ultrasounds-assisted pepsin hydrolysates showed the highest values of antioxidant and inhibitory activity of ACE-I, ECE-I, DPP-IV, and neprilysin enzymes, and therefore these hydrolysates were characterised to obtain the bioactive peptide profiles. A total of 995 peptides were identified by tandem mass spectrometry in the most active fractions separated by reversed-phase liquid chromatography, and 20 of them were predicted as potentially responsible for the observed bioactivities and subsequently characterised by in silico tools. These results evidenced the potential of hydrolysis pre-treatments with pepsin and ultrasounds-assisted pepsin to obtain beef liver hydrolysates rich in bioactive peptides that could exert multifunctional bioactivities such as antioxidant, antihypertensive, and antidiabetic, while giving added value to this meat by-product.

Film and Television Animation Production Technology Based on Expression Transfer and Virtual Digital Human

The world of film and TV animation has witnessed a revolutionary transformation with the combination of Expression transfer and digital virtual Human technology. This paper delves into the superior methodologies and technological improvements in the discipline of animation production, especially specializing in how those technology are redefining the requirements and practices of animation in film and television. Expression transfer technology, a groundbreaking approach in animation, entails the transfer of facial expressions from real actors to lively characters. This technique not most effective enhances the realism of lively characters but additionally lets in for a extra nuanced and emotive performance, bridging the space among conventional animation and stay-action performances. Digital Human era, however, entails creating extraordinarily practical virtual representations of people. Those digital humans aren’t mere caricatures or stylized variations however are reasonable in appearance and movement, way to advancements in motion seize, 3-D modeling, and synthetic intelligence. The mixture of those technologies is main to a new technology in animation where characters aren’t only visually stunning however also exhibit a depth of emotion and realism previously unimaginable. This paper explores diverse case research and applications of these technology in current animation, highlighting their effect on storytelling, person improvement, and viewer engagement. It also addresses the demanding situations and moral considerations in employing these technologies, which includes retaining artistic integrity and the capacity for misuse. The research concludes with a forward-searching attitude on how Expression transfer and digital digital Human technologies are set to redefine the future of movie and television animation, presenting new opportunities for creative expression and narrative intensity.

Heat Stress in Beef Cattle: Climate Change and the Global Scenario – A Review

Abstract With the increasing human population and urbanization, the demand for animal origin products is going to grow, especially in the developing nations till the 2050s and the production needs to be escalated and optimized with the changing climate. Heat stress is known to reduce the animal performance, production, shelf life and meat quality in all animals. The beef cattle are globally reared, following different managemental practices, so the usage of natural resources like land and water, manpower, fodders, production systems and the environmental impact also varies profoundly. Recent changes in the climate, global warming and depletion of resources have severely affected the production and heat stress is now a common constraint all over the world. Due to evolutionary diversification the tropical and temperate breeds are comparatively more thermotolerant, but the beef cattle in the colder regions are vulnerable to high environmental temperatures. Also, the production of beef increases the carbon footprint and is much less eco-friendly than growing plant-based protein. So, we comprehended the environmental temperature variation over the continents and impact of heat stress on beef cattle. Also, other factors like cattle population, land and pasture usage, livestock units in trade, methane emissions and gross beef production value were examined to evaluate the collective impact of all these on the beef sector. Our findings and predictions reveal that, in the advent of climate change, depleting natural resources and rise in the greenhouse gases, beef production will be a constant challenge, which can be only achieved by maintaining a healthy cattle population and optimum usage of natural resources. Only then can the beef sector be efficient, sustainable, and a profitable enterprise in future.

Batch-to-Batch Differences in Nutritional Quality of Selected Raw and Cooked Pork Offal

With a pressing need to enhance sustainability and reduce waste, harnessing the untapped nutritional wealth buried in by-products of the meat processing industry is more critical than ever. The research was aimed at comparing variation in raw material between batches and establishing a comprehensive database that would serve as a valuable resource for manufacturers seeking to exploit the potential of pork offal. This study is focused on analysing both raw and cooked offal, including liver, heart, kidney, spleen, blood, tongue, esophagus, as well as meat trimmings. Four batches were analysed with regard to their chemical composition, micro- and macro-element content, amino as well as fatty acid profile. The results indicate significant differences in raw material supplies from the same supplier across batches. In our study, all analysed pork offal and meat trimmings exhibited statistically significant variations between individual batches. Furthermore, clustering analysis conducted on the data will be beneficial in optimising recipes and formulations that utilise pork offal. This research may contribute to the sustainable utilisation of meat by-products, waste reduction and overall value enhancement in the meat processing industry.

Morphomics, Survival, and Metabolites in Patients With Metastatic Pancreatic Cancer

Associations of body mass index (BMI) with survival in pancreatic ductal adenocarcinoma (PDA) have substantial variability in literature, potentially due to heterogeneous patient populations and retrospective analyses. Additionally, BMI may inadequately describe body composition (ie, morphomics; including subcutaneous and visceral fats, muscle, and fascia), which might have independent biological roles and associations with survival. To study the associations of BMI and morphomics with survival and metabolomics in metastatic PDA. This cohort study prospectively collected patient data, imaging, and serum on the phase 3 trial (Avenger500), which investigated the efficacy and safety of 5-fluorouracil, leucovorin, oxaliplatin, and irinotecan (FOLFIRINOX) versus modified FOLFIRINOX plus devimistat. The randomized trial accrued 528 patients with chemotherapy-naive, metastatic PDA from Europe, Israel, Korea, and the US between 2018 and 2020. In the present study, per-protocol patients with L1 to L4, T10 to T12 vertebral levels were evaluated. Data analysis occurred from January 2023 to April 2024. Patient data were collected by clinical staff. Morphomics were analyzed from baseline imaging. Metabolites were extracted from baseline serum. A multifaceted statistical approach evaluated associations of BMI and morphomics with progression-free survival (PFS) and overall survival (OS). Associations of morphomics with metabolites were also studied. Of the 528 initial patients, 476 (median [IQR] age, 63 [56-68] years; 280 male [58.8%]; median [IQR] BMI, 25.0 [22.1-25.9]) were evaluable for the present study. BMI (obese [≥30] compared with normal [18.5-24.9]) was not associated with OS (hazard ratio [HR], 0.90; 95% CI, 0.67-1.22; P for trend = .33). More subcutaneous fat was associated with longer OS (HR, 0.62; 95% CI, 0.41-0.94; P for trend = .02). Higher visceral fat density was associated with shorter PFS (HR, 1.74; 95% CI, 1.23-2.48; P for trend = .002) and OS (HR, 1.50; 95% CI, 1.12-2.00; P for trend = .008). A higher muscle-to-fascia ratio was associated with longer PFS (HR, 0.58; 95% CI, 0.40-0.84; P for trend = .005) and OS (HR, 0.56; 95% CI, 0.41-0.75; P for trend = 1.7 × 10-4). Subcutaneous fat was positively associated with long-chain fatty acid metabolism including pristanic acid, decanoylcarnitine, decenoylcarnitine, and octanoylcarnitine. Muscle-to-fascia was positively associated with metabolites including acetylcarnosine (β = 0.34; 95% CI, 0.21-0.47; P = 1.27 × 10-6). In cohort study of patients with metastatic PDA, BMI was not associated with survival. Higher visceral fat density, subcutaneous fat area, and muscle-to-fascia ratio were associated with survival independent of BMI. The latter 2 were associated with higher levels of animal product metabolism. These findings could represent novel focuses for prognostication and intervention to improve survival of patients with PDA.

Prospects and Application of Solid-State Fermentation in Animal Feed Production – A Review

Abstract Animal feed production has recently received significant attention in the food and livestock sectors due to its high nutritional value and substantial environmental potential. Extensive studies have been conducted to explore the effects of solid fermented feeds on different growth stages of animals but also on the health status of animals, the quality of animal products, and the composition of intestinal micro-flora to replace non-fermented feed production. The purpose of this review is to provide up-to-date existing trends, recent developments, and prospects of solid-state fermentation (SSF) practices for the production of animal feeds. Studies on enhancing nutritional factors by increasing the crude protein content, enzymes, and antioxidant activity of feed using physical processing methods on agro-industrial waste such as rapeseed meal, cottonseed, wheat bran, soybean meal, and legumes by mainly SSF are reviewed and discussed thoroughly.

Simultaneous determination of some coccidiostat antibiotics in food by liquid chromatography tandem mass spectrometry (LC-MS/MS)

Coccidiostats (anticoccidial antibiotics) are veterinary drugs widely used for the prevention and treatment of coccidiosis, especially in poultry farming. The illegal use of anticoccidial antibiotics, in the wrong dosage and duration, can lead to antibiotic residues in animal products and harm the health of consumers. In this study, a highly sensitive and accurate liquid chromatography-tandem mass spectrometry method was developed for the simultaneous analysis of anticoccidial antibiotics (diclazuril, nicarbazin, robenidin and salinomycin) in food. Samples were extracted using the QuEChERS method with acetonitrile (ACN) as the extraction solvent and cleaned with C18 adsorbent. The cleaned extracts were analyzed on an LC-MS/MS system with a C18 reversed-phase chromatography column (2.1 x 150 mm, 3.5 µm) and corresponding pre-column. The mobile phase used was 0.1% HCOOH in water and methanol (MeOH), the flow rate was 0.5 mL/min. The method had good specificity, the linear range was established in the range of 10-100 ng/mL, the correlation coefficient R2 > 0.998. The method recovery was in the range of 81-109% and the relative standard deviation (RSD%) was in the range of 1.77-10.13% for milk and meat samples. The limit of quantification and limit of detection of the method for four coccidiostat antibiotics were 3.0 µg/kg and 10 µg/kg, respectively. The method was applied to analyze the content of these four antibiotics in 31 food samples (meat and meat products, milk and dairy products) randomly purchased in Hanoi. The results did not detect four coccidiostat antibiotics in all 31 samples, initially showing that the residual contamination of coccidiostat antibiotics is basically safely controlled.