Foods Of Animal Origin Research Articles

Evaluating the contribution of antimicrobial use in farmed animals to global antimicrobial resistance in humans

Antimicrobial resistance (AMR) is currently regarded by the World Health Organization (WHO) as one of the most significant risks to global public health. The most critical causes of AMR infections in humans are the misuse and overuse of antimicrobials in humans and farmed animals. The rising global demand for food of animal origin encourages the increase of animal production worldwide, especially in developing countries. Simultaneously, current farming practices often extensively use antimicrobials on animals, influencing bacterial AMR incidence. This study aims to evaluate the correlation between antimicrobial use (AMU) in farmed animals and the detection of AMR infections in humans, the effects of enforcing laws in animal farming in a country on AMR situation in the neighbors, and the potential of AMR to spread from one country to another. Using data from 30 largest animal-producing countries in different regions of the world, between 2010 and 2020, and a Spatial Durbin Model (SDM), we found that AMU in farmed animals increases AMR in humans and there is a spatial dependence between countries regarding AMR spreading. Such findings indicate that a globally coordinated strategy regulating AMU on farmed animals may reduce AMR emergence and worldwide spreading.

Total Lactic Acid Bacteria (LAB) and Antioxidant Activity of Goat's Milk Yoghurt with The Addition Of Red Bran During Refrigeration Storage

Background: Goat milk yogurt is a functional food made from processed food of animal origin with the help of LAB fermentation, which is healthy for the body. The utilization of red rice bran flour as an additional ingredient in making yogurt can increase the level of antioxidant activity and become a food fiber content in yogurt. Purpose: This study aims to determine the effect of refrigerated storage temperatures (4℃) of goat milk yogurt with the addition of red rice bran flour on total LAB and antioxidant activity. Methods: Using a CRD with five treatments and four replications. The treatment included 0, 1, 2, 3, and 4 weeks of storage. Each treatment group will be calculated for total LAB using the TPC method and antioxidant activity testing using the DPPH method. The data obtained were analyzed using the One Way ANOVA Test followed by the Duncan Test to determine the effect between treatment groups. Results: The results showed that storage time significantly affected (p<0.01) total LAB and antioxidant activity. The results of total LAB successively from 0 to 4 weeks of storage were 8,0 x 108 CFU/ml, 7,8 x 107 CFU/ml, 7,6 x 107 CFU/ml, 6,4 x 106 CFU/ml, and 6,3 x 106 CFU/ml. The results of antioxidant activity successively from 0 to 4 weeks of storage were 534,29 mg/ml, 443,96 mg/ml, 358,61 mg/ml, 282,32 mg/ml, and 230,88 mg/ml. Conclusion: Goat’s milk yogurt with the addition of brown rice bran flour is recommended to be consumed for up to 2 weeks of storage.

Cereal-based fermented foods as a source of folate and cobalamin: The role of endogenous microbiota

Folate (vitamin B9) and cobalamin (vitamin B12) deficiencies potentially affect millions of people worldwide, leading to different pathologies. In Ethiopia, the diet is characterized by high consumption of fermented cereal-based foods such as injera, a good source of folate but not of cobalamin, which is only found in foods of animal origin that are rarely consumed. Some of the bacteria responsible for the fermentation of cereals can synthesize cobalamin, but whether or not fermented cereal food products contain cobalamin remains underexplored. The objective of this study was to assess the folate and cobalamin content of injera collected from various households in Ethiopia at different stages of production. Global (16S rRNA gene sequencing) and specific (real-time PCR quantification of bacteria known for folate or cobalamin production) bacterial composition of these samples was assessed. UPLC-PDA was used to identify the cobalamin to see whether the active or inactive form was present. Surprisingly, teff flour contained 0.8 μg/100 g of cobalamin, most probably due to microbial contamination from the environment and the harvesting process. While fermentation increased the folate and cobalamin content in some households, their levels decreased in others. Conversely, cooking consistently reduced the level of the vitamins. Fresh injera contained, on average, 21.2 μg/100 g of folate and 2.1 μg/100 g of cobalamin, which is high, but with marked variation depending on the sample. However, the form of cobalamin was a corrinoid that is biologically inactive in humans. Injera fermentation was dominated by lactic acid bacteria, with significant correlations observed between certain bacterial species and folate and cobalamin levels. For example, a high proportion of Fructilactobacillus sanfranciscensis, a known folate consumer, was negatively correlated with the folate content of injera. On the contrary, Lactobacillus coryniformis, known for its cobalamin synthesis ability was present in high proportion in the cobalamin-rich samples. These findings highlight the complex interrelationship between microorganisms and suggest the involvement of specific bacteria in the production of folate and cobalamin during injera fermentation. Controlled fermentation using vitamin-producing bacteria is thus a promising tool to promote folate and cobalamin production in fermented food.

Simultaneous determination of triazole fungicides in animal-origin food by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry

A method for the simultaneous determination of 21 triazole fungicides in animal-origin foods was established by using UPLC–MS/MS. The dilution solvent, extraction solvent, and QuEChERS purification adsorbent composition, were optimized. The response value of the target compound was the highest and the chromatographic peak shape was optimal under the following conditions: water-acetonitrile as the mobile phase, acetonitrile to extract the target compound, C18 (100 mg) as the adsorbent, and water-acetonitrile as the diluent. Our method was validated under electrospray ionization (ESI) + conditions with six animal-origin foods. The 21 triazole fungicides showed good linear relationships (0.1–20 μg∙L−1, R2 > 0.99). The limits of detection and quantitation ranged from 0.1 to 0.3 μg∙kg−1 and 0.3 to 0.9 μg∙kg−1, respectively. The average recoveries ranged from 72.0% to 114.8% with RSDs < 9.9%. Therefore, our method was suitable for the determination of pesticide residues in commercially available animal-origin samples.

ANALYSIS AND IMPACT OF APIA ACTIVITY ON DEVELOPMENT AND SUPPORT OF AGRICULTURAL SECTOR IN ROMANIA

Agriculture is a very important sector for Romania, providing food and products of vegetable and animal origin, jobs for people in villages and business opportunities for enterprises and investors. Romania's agricultural area is in permanent change, influenced by European money that was attracted through support measures and community policies, and environmental protection and the need to ensure a sustainable development of agriculture in Romania made the activity of the Agency for Payments and Intervention for Agriculture (APIA) stands out as an important factor in aligning modern agriculture with the environment. Through this study, we aim to analyze the way in which APIA promotes sustainable agricultural practices and provides support to farmers to protect the environment and whether the agency's activity has brought benefits to the economic development of Romania and Neamț County. We thus demonstrate the important role that the agency has for the economy, for supporting farmers and agricultural producers in Romania, but also for the country's agricultural policy, the institution participating in the development and implementation of national agricultural policies and strategies, as well as in the process of negotiating subsidies and projects within the European Union (EU) agricultural financing system.

Evaluation of effectiveness of school-based nutrition education in improving the consumption of pulses-based food among female adolescents in Northwest Ethiopia: a cluster randomized controlled trial

BackgroundProtein undernutrition is a prevalent health problem in Ethiopia severely affecting the reproductive outcome of women. This is mainly because of inadequate consumption of protein due to the high cost of animal-origin food and the lack of knowledge about the benefits and the methods of preparation of pulses-based foods. Therefore, this trial was conducted to evaluate the effectiveness of nutrition education in improving the consumption of pulses-based foods among female adolescents.MethodsA two-arm pragmatic cluster randomized controlled trial was conducted among female adolescents in Northwest Ethiopia. Clusters were schools assigned into intervention and control groups by cluster randomization. The trial participants were female adolescents. The intervention was pulses-based nutrition education, and the comparator was the usual dietary practice of adolescent girls. The education was delivered over four weeks on a 45–60 min session per week basis. The primary outcome of the intervention was pulses-based food consumption, and the secondary outcomes were knowledge and attitude about pulses food. Data on the outcome and the confounding variables were collected at baseline and end-line of the intervention. The analysis was based on intention-to-treat analysis, and a log-binomial logistic regression model was fitted to the data to calculate relative risk with the corresponding p value adjusted for baseline characteristics. The intervention was considered effective when the p value was < 0.05.ResultsA total of 269 intervention and 278 control participants from the four clusters completed the trial making response rates of 92.1% and 95.2%, respectively. The pulses-based nutrition education enabled participants in the intervention group to maintain their pulses-based food consumption state, while participants in the control group significantly reduced their consumption by about threefold [ARR; 95% CI 2.99 (1.87, 4.79)] from harvesting to non-harvesting season. The consumption of pulses-based food was higher by 16% among the intervention participants as compared to the control participants [ARD; 95% CI 0.16 (0.10, 0.21)].ConclusionPulses-based nutrition education is effective in improving the consumption of pulses-based food among female adolescents. Therefore, policies and strategies are required to integrate this intervention in the school nutrition program.Trial registration: The trial was registered in the Pan African Clinical Trials Registry (PACTR202111813445259) on 02 November 2021.

Комплексная оценка продовольственного обеспечения населения Республики Башкортостан в условиях новых системных вызовов

Abstract. The aim of the study is to carry out a comprehensive assessment of the food supply of the population of the federal subject (on the example of Bashkortostan) in the context of external and internal systemic challenges and restrictions. The article updates that the observed conditions for the development of the agro-food system at the national level and at the level of federal subjects are accompanied by unprecedented changes that are directly related to the emergence of new challenges, trends and threats, the active introduction of innovative and digital technologies in food of animal origin. The conceptual and theoretical and methodological basis of the study was the fundamental provisions of modern theories of the development of the agro-food market and food supply for the population, human capital, economic growth and sustainable functioning of rural territorial entities. Methods. As a means of instrumental and methodological apparatus of research, a systematic approach was used in combination with the following methods: monographic, abstraction, graphic visualization, generalization and systematization, comparative analysis. A comprehensive study of food supply for rural and urban residents of the Republic of Bashkortostan is based on the results of a content analysis of current trends, problems and strategic prospects for the development of agriculture and the agro-food market in the region. Results. In this study, a comprehensive assessment of the provision of the population of the Republic of Bashkortostan with food of animal origin (meat and meat products, milk and dairy products) was carried out. The key problems hindering the development of the livestock and poultry complex of the republic are identified. The dynamics of indicators reflecting the number of livestock, the productivity of livestock and poultry in the agro-formations of the region is considered. An assessment of individual social indicators of the functioning of rural territorial entities at the regional level is given. A detailed analysis of the balance of resources (production, imports and exports) of meat and meat products, milk and dairy products, formed on a republican scale, was carried out. Practical proposals have been developed aimed at improving the criteria and parameters of self-sufficiency of the population of the federal subjects of our country with certain types of agro-food products. The scientific novelty lies in the substantiation of new provisions aimed at the effective assessment of the food supply of the population of the federal subject with food of animal origin in the face of modern challenges, threats and restrictions.

The Sosialisasi Pangan Darurat: Pengasapan Pangan Asal Hewani di Kelompok Sari Tani Kecamatan Cugenang Kabupaten Cianjur

Cianjur is a high-risk area for seismic disasters, so it is important to pay attention to emergency food supplies. One type of emergency food that could be developed is smoked foods of animal origin (PAH). The activities carried out are aimed at generating enthusiasm and enhancing the skills of the community so that they can continue to process food products of animal origin and can produce them independently. So that Cianjur, especially Cugenang district, becomes a disaster-resilient area. future area. PKM activities were carried out on August 18, 2023 at Sari Tani Group's business location, Wangun Jaya Village, Cugenang District, Cianjur, West Java, with Sari Tani Group partners. The steps taken to implement PKM activities included discussion, socialization, and evaluation of the activities (pre-test and post-test). The results of PKM activities show a change in the level of understanding of participants about influencing factors, processing steps and methods that can be applied in smoking technology of original food products. animals, from 20% to 93.33%. PKM results also showed changes in participants' level of knowledge about correct methods according to regulations of the US Food and Drug Administration (FDA), and the role of smoke chemicals in the process. disinfection and any negative effects that may arise from root-based disinfection technology from 6.67% to 86.67%.

Exposing Salmonella Senftenberg and Escherichia coli Strains Isolated from Poultry Farms to Formaldehyde and Lingonberry Extract at Low Concentrations.

European Union (EU) countries strive to improve the quality and safety of food of animal origin. Food production depends on a good microbiological quality of fodder. However, feed can be a reservoir or vector of pathogenic microorganisms, including Salmonella or Escherichia coli bacteria. Salmonella spp. and E. coli are the two most important food-borne pathogens of public health concern. Contamination with these pathogens, mainly in the poultry sector, can lead to serious food-borne diseases. Both microorganisms can form biofilms on abiotic and biotic surfaces. The cells that form biofilms are less sensitive to disinfectants, which in turn makes it difficult to eliminate them from various surfaces. Because the usage of formaldehyde in animal feed is prohibited in European countries, the replacement of this antibacterial with natural plant products seems very promising. This study aimed to assess the inhibitory effectiveness of Vaccinium vitis-idaea extract against biofilm produced by model Salmonella enterica and E. coli strains. We found that formaldehyde could effectively kill both species of bacterial cells in biofilm, while the lingonberry extract showed some antibiofilm effect on S. enterica serovar Senftenberg. In conclusion, finding natural plant products that are effective against biofilms formed by Gram-negative bacteria is still challenging.

Polar pesticides in food of animal origin: interlaboratory validation to evaluate method fitness-for-purpose of official control

The present work reports on the design, execution and evaluation of results of an interlaboratory validation study aimed at verifying the fitness-for-purpose of a LC-MS/MS method for the detection of polar pesticides in food of animal origin in official control and monitoring programmes. To this scope, five participant laboratories, with relevant expertise, were recruited. After passing a pre-trial test, the participants were asked to analyse test samples of bovine fat, chicken eggs and cow’s milk, contaminated with 11 polar pesticides (group A: Aminomethyl phosphonic acid (AMPA), cyanuric acid, ethephon, glyphosate, fosetyl aluminium, 2-hydroxyethyphosphonic acid (HEPA), maleic hydrazide, N-acetyl-glyphosate, group B: N-acetyl glufosinate (NAG), 3-methylphosphinicopropionic acid (MPP) and glufosinate ammonium) at two different levels (0.05 and 0.25 mg/kg−1 and 0.01 and 0.05 mg/kg−1 for group A and B respectively. The method was based on acidified methanol/water extraction followed by dSPE clean up with C18 sorbent. For LC-MS/MS analysis isotopically labelled standards were used for all targeted analytes. With a couple of exceptions, average recoveries ranged from 85% to 110%, with repeatability (RSDr) ranging from 3% to 25%, and reproducibility (RSD R ) from 4% to 26%. The assessment by different laboratories provided also insights on key factors impacting method performance characteristics and its implementation by new users.

Determination of pathogens and contamination indicators in fresh pork sausages in the state of São Paulo

The highest cost of beef and chicken meat justifies higher consumption of fresh sausage products, especially linguiça, due to their easy preparation and affordability for consumers, making necessary an evaluation of sanitary hygienic conditions of these products. The objective was to investigate the presence of pathogens such as Salmonella spp., coagulase-positive Staphylococcus, thermotolerant coliforms, Escherichia coli and Campylobacter spp. in artisanal and inspected fresh pork sausages. It was found that 12% (6/50) of the artisanal sausage samples were contaminated with Salmonella spp., 58% (29/50) presented coagulase-positive Staphylococcus levels above the acceptable limits for consumption and 76% (38/50) presented thermotolerant coliform levels above the acceptable limits. In sausage samples produced under inspected conditions, 6% (3/50) were contaminated with Salmonella spp., 24% (12/50) presented thermotolerant coliform levels above the acceptable limits, 2% (1/50) presented enteropathogenic E. coli. None samples showed coagulase-positive Staphylococcus counts above the limits, or presence of Campylobacter spp. Sensitizing traders and consumers about the importance of inspection service in food of animal origin is urgent for a sanitary acceptable production, since foodborne diseases continue to be a public health problem.

African Swine Fever and Its Socio-Economic Impacts in Indonesia

African Swine Fever Virus (ASF) is a viral disease in pigs that affects the supply of food of animal origin. ASF is caused by a double-stranded DNA virus of the family Asfarviridae and genus Asfivirus, which can be transmitted and causes various bleeding events in the internal organs and is accompanied by a very high mortality rate. This disease is generally characterized by fever, lack of appetite, and bleeding from the skin, back, and legs. Even though this virus is not zoonotic and cannot be transmitted to humans, it is fatal and causes tremendous economic loss. This disease spread throughout Asia in a relatively short time in 2019, and it has even been reported that this disease has entered Indonesia since the end of 2019. The impact of this infection resulted in socioeconomic changes in both rural and urban communities, which could then affect the country's economy at the macro level. Several countries are needed in their efforts to control ASF, one of which is to increase quarantine, limit the traffic of pigs, and reduce the population of sick pigs exposed to the ASF virus.

Carbon dots-based fluorescent molecularly imprinted photonic crystal hydrogel strip: Portable and efficient strategy for selective detection of tetracycline in foods of animal origin

Rapid, portable, and sensitive detection of tetracycline (TC) is crucial for the environment and human health. In this study, we developed carbon dots (CDs)-based fluorescent molecularly imprinted photonic crystal hydrogel (FMIPH) strips for TC detection in animal-derived foods. CDs emit fluorescent signals, and molecularly imprinted polymers provide specific recognition sites for TC. Inverse opal photonic crystals afford stable 3D macroporous mass transfer channels that considerably reduce binding time between TC and the strips. The portable FMIPH strip exhibited a linear fluorescence response to TC in the concentration range of 0.1–50 μg mL−1, with a detection limit of 0.067 μg mL−1. Good recoveries of TC (93.86–112.59%) were observed in TC-spiked commercially available pork, eggs, and milk. A combination of FMIPH strips with a portable fluorescent reading device could achieve sensitive, on-site, and real-time detection of TC in animal-derived foods.

Levels of caesium-137 in food of animal origin in Poland.

Radioactive contamination of the environment is one of the greatest threats after a nuclear accident due to released radionuclides. From a radiotoxicological point of view, the most important radionuclide is caesium-137. Formed mainly during nuclear explosions, caesium-137 can persist in the soil for many years, from where it constantly enters the food chain. One of the elements of ensuring food safety is the monitoring of its radioactive contamination, mainly with radioactive caesium isotopes. The aim of the study was to determine the content of caesium-137 in food of animal origin. A total of 1,416 muscle samples from cattle, sheep, pigs, game and fish, as well as chicken eggs and dairy products were examined using gamma-ray spectrometry. Caesium-137 activities ranged from below the minimum detectable activity concentration (MDC) to over 4,000 Bq/kg wet weight (w.w.). Most often, the values did not exceed the MDC or were in a range below 100 Bq/kg. The exception was the muscle tissue of game animals, especially wild boar, where a significant activity of caesium-137 was recorded, the highest of which was 4,136.8 ± 238 Bq/kg w.w. Committed effective doses determined for each matrix ranged from 0.01 to 0.83 µSv/kg, with the highest value determined for wild boar. The calculated exposure doses with values well below the accepted low radiation dose (100 mSv) did not indicate any significant amounts of ionising radiation from the food consumed.

Determination of 10 carbamate pesticide residues in liquid milk by ultra performance liquid chromatography-tandem mass spectrometry with pass-through solid-phase extraction purification

Carbamates are used in broad-spectrum insecticides and herbicides, and have highly efficient, low-residue, and long-lasting characteristics. However, this type of pesticide exerts mutagenic, teratogenic, carcinogenic, and other adverse effects, and its frequent use can exceed the recommended scope and limits. Research on the determination of carbamate pesticides mainly focuses on foods of plant origin and pays less attention to foods of animal origin. The methods for carbamate determination described in the current national standards have complicated operating procedures and low efficiency. Therefore, highly efficient and accurate methods for carbamate detection in milk must be established. In this work, a rapid method based on pass-through solid-phase extraction (SPE) purification coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of 10 carbamate pesticides in liquid milk. The pretreatment and instrument methods were systematically optimized. The milk sample was extracted with acetonitrile, and then purified using a Captiva EMR-Lipid filtration kit. The purified extract was separated on an ACQUITY UPLC BEH C18 column with mobile phase of methanol and 0.1% formic acid aqueous solution in gradient elution. The flow rate was 0.3 mL/min. Column temperature was 35 ℃. Quantitative analysis was performed using the external standard method with matrix matching curves. The 10 carbamate pesticides showed good linear relationships in the mass concentration range of 2-200 μg/L, with correlation coefficients greater than 0.999. The limits of detection (LODs) and quantification (LOQs) for the 10 carbamate pesticides were 0.045-0.23 and 0.15-0.77 μg/kg, respectively. Recovery tests were conducted using the blank-matrix method at three spiked levels of 15, 50, and 100 μg/kg, and good recoveries for the 10 carbamate pesticides were obtained. In particular, the recoveries for the three spiked levels of 15, 50, and 100 μg/kg were 68.7%-93.3% with relative standard deviations (RSDs) of 1.8%-8.0%. The proposed method is efficient, convenient, accurate, and suitable for the rapid detection of the 10 carbamate pesticides in liquid milk. Compared with the conventional NH2 and ENVITM-18 SPE columns used in the national standard determination method, the proposed method demonstrated better purification effects. The recoveries for aldicarb sulfoxide, aldicarb sulfone, methomyl, and carbaryl after purification using the Captiva EMR-Lipid kit increased from 60% to 80%. Thus, the proposed method is suitable for targets with strong polarity and gives measurement results with good repeatability and accuracy.

Experimental Study on the Transfer of Polychlorinated Biphenyls (PCBs) and Polychlorinated Dibenzo-p-dioxins and Dibenzofurans (PCDD/Fs) into Milk of High-Yielding Cows during Negative and Positive Energy Balance.

Dioxin-like polychlorinated biphenyls (dl-PCBs) as well as polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) are a major concern for food safety, especially in fat-containing foods of animal origin, such as milk. Due to the lipophilic character of PCDD/Fs and PCBs, it is of special interest to explore whether the metabolic state of high-yielding cows influences the transfer rates into milk. Five German Holstein cows were orally exposed to a mixture of 17 PCDD/Fs, 12 dl-PCBs, and 6 non-dioxin-like PCBs (ndl-PCBs) for two dosing periods of 28 days each. The first period covered the negative energy balance (NEB) after calving, while the second period addressed the positive energy balance (PEB) in late lactation. Each dosing period was followed by a depuration period of around 100 days. During the NEB phase, the transfer rates of 14 PCDD/Fs and 7 dl-PCBs quantified were significantly (p ≤ 0.1) higher compared to the PEB phase, indicating an influence of the metabolic state on the transfer. Furthermore, the congener-specific transfer rates (0.3-39%) were in the range of the results from former studies. This indicates that the milk yield of the exposed cows is not the only determining factor for the transfer of these congeners into milk.

Determination of Xylazine and Its Metabolite in Milk by a Molecularly Imprinted Microsphere Based Pseudo Immunoassay

The residues of xylazine and its metabolite in foods of animal origin are dangerous to consumers, but there has been no enzyme-linked immunosorbent assay (ELISA) or ELISA-like method reported to determine them so far. In this study, molecularly imprinted microspheres with xylazine as the template were synthesized, and 2,6-dimethylaniline (the metabolite of xylazine) was used as a hapten to prepare a streptavidinated horseradish peroxidase-labeled conjugate. A direct competitive pseudo-ELISA was developed on a microplate for xylazine and 2,6-dimethylaniline in milk. Because biotinylated horseradish peroxidase was employed to enhance the signal, the limits of detection for xylazine (0.003 ng/mL) and 2,6-dimethylaniline (0.005 ng/mL) were increased by 16.7- and 14-fold, respectively, compared to a horseradish peroxidase-labeled conjugate. Moreover, this material may be reused three times. The analysis of milk samples was consistent with values obtained by liquid chromatography tandem mass spectrometry. This is the first paper reporting a molecularly imprinted polymer-based method for the determination of xylazine and its metabolites in food.

Juridical Analysis Of The Authority Of Quarantine Officers In Handling Illegal Importation Of Caws On Batam Island

Quarantine is a system of preventing the entry, exit, and spread of quarantined animal pests and diseases, as well as supervision and control of food safety and food quality, feed safety and feed quality, Genetic Engineering products, Genetic Resources, Biological Agents, Invasive Foreign Species, Wild Plants and Animals, and Rare Animals that are introduced into, spread from one area to another area, and removed from the territory of the Unitary State of the Republic of Indonesia. To maintain, protect, and improve the quality of animal/livestock resources, animal/livestock health, animal welfare, and the provision of safe, healthy, whole, and halal (ASUH) food of animal origin, as well as maintaining peace or eliminating/preventing public unrest, it is necessary to provide guidance, regulation, control, and supervision of livestock businesses and animal health services. To create a conducive business climate and legal certainty in animal husbandry and animal health, it is necessary to regulate the mechanisms and procedures for licensing livestock and animal health businesses in the regions. The problem faced by the Batam Class I Agricultural Quarantine Center is caused by cows entering Batam Island that do not have an entry permit and are not by Law Number 21 of 2019, which states that every entry or release must be carried out through a designated Place of Entry and Place of Expenditure.

Concentrations, Profiles, and Health Risks of Organic Ultraviolet Filters in Eight Food Categories Determined through the Sixth Chinese Total Diet Study.

Ultraviolet (UV) filters are emerging contaminants that have been found in high concentrations in human tissues. Food intake is generally considered to be the primary route of human exposure to contaminants. In this study, 184 composite food samples, prepared from 4268 individual samples in eight categories collected from 23 Chinese provinces for the sixth Chinese total diet study, were analyzed. The total and median UV filter concentrations in food samples were 1.5-68.3 and 7.9 ng/g wet weight, respectively. The highest median concentrations were found in decreasing order in meat, cereals, and legumes. In total, 15 UV filters were analyzed. 2-Ethylhexyl salicylate, homosalate, and 2-ethylhexyl-4-methoxycinnamate were dominant and made median contributions of 34.1%, 22.6%, and 14.5%, respectively, and 2-(2H-benzotriazol-2-yl)-4,6-di-tert-pentylphenol contributed the median of 0.03%, of the total UV filter concentrations. The estimated total daily UV filter intake in animal-origin foods and total UV filter concentration in human milk from the same province were significantly correlated (r = 0.44, p < 0.05). Predicted absorption, distribution, metabolism, and elimination properties led to 10 UV filters being prioritized as most likely to be retained in human tissues. The prioritization results and toxicity assessments indicated that octocrylene and 2-ethylhexyl-4-methoxycinnamate have stronger effects in vivo and therefore require more attention than others.

A green deep eutectic solvent based dispersive liquid-liquid microextraction for the quantitative analysis of 21 polychlorinated biphenyl metabolites in food of animal origin using injector port silylation-gas chromatography-tandem mass spectrometry

An analytical method was developed for the quantitative determination of 21 polychlorinated biphenyls (PCBs) metabolites (17 were -OH, 1 -MeO, and 3 were MeSO2) in foods of animal origin using deep eutectic solvent (DES) based dispersive liquid-liquid microextraction followed by injector port silylation-gas chromatography-tandem mass spectrometry. The type of DES (thymol: camphor, 1:1 molar ratio) and optimum volume of DES (300 µL), pH (7.0), and disperser solvent (acetonitrile) were optimized to attain the maximum extraction efficiency. The limit of detection, limit of quantification, and percent recovery were found to be in the range of 0.12–0.23 ng/mL, 0.40–0.76 ng/mL, and 80.1–111.4%, respectively. The expanded uncertainty was observed to be in the range of 7.2–22.8% for the targeted analytes. The proposed method was applied to real food samples (milk, meat, fish, and egg) and the levels were found to be in the range of 0.64–32.14 ng/g. This is first of its kind method using green solvent based method for the analysis of PCB metabolites (-OH, MeO, and MeSO2) and will find extensive application in routine testing for foods of animal origin.