Animal Organism Research Articles

Lipids in the reproduction of sires.

The main class of nutritional interest for lipids are fatty acids (FA), which correspond to 90% of triglycerides, the main form of lipid storage in both plants and animals. FAs serve as a source of energy in the diet of cattle; however, they also have an important non-caloric effect on animal organisms as they are important components of the physical and functional structures of cells and participate in the composition of steroid hormones. As such, research has studied the improvement of semen quality through the provision of polyunsaturated FAs in bull diets, as well as the use of FAs in semen extenders in order to reduce damage to sperm cells, which can alter lipid composition and the quality of frozen sperm. Therefore, the objective of this work was to review the effectiveness of lipids on reproductive efficiency, based on their effects on semen quality and hormonal production. Supplementation with polyunsaturated FAs positively alters semen composition and in vitro fertility; however, results vary according to the type of FA used, the method of administration, and its quality. Fish oil and linseed oil showed better results in qualitative parameters in fresh and thawed semen. The use of cyclodextrins to incorporate or extract cholesterol from plasma membranes can also improve the viability of cryopreserved semen.

Tomás Saraceno: semiotic regimes of posthuman temporalities

Abstract Since 2007, Tomás Saraceno has been developing a project that aims to break out of the anthropocentric understanding of communication and coexistence with other animal organisms. In this article, I point out the importance of using modern visualization technologies to analyze and investigate the structure of communication frameworks and their modalities in the animal world, specifically using the example of spiders. The visualization of what is normally invisible to the eye and inaudible to the ear rearticulates the realm of the visible and the audible, which Saraceno then uses for art-critical purposes, with the aim of forming a new type of posthuman attunement to the environment and other animal species. The condition for outlining and articulating Saraceno’s artistic vision is the use of the latest technologies of visualization, which operate on the principle of digital and algorithmic code. They synthesize different planes of affectivity and transform, modulate, and combine the different modes of signs into a single technical-organic complex: they offer new ways of seeing and feeling the world. Thus, hybrid ecology in Saraceno’s case does not imply a return to primordial ways of life, but on the contrary, it forms a semiosphere attuned to a specific plane of consistency that enfolds distinct modes of signs and develops them into an expression of hitherto unsuspected variants of life.

Immune and antioxidant status of the body of lambs using brown algae <i>Fucus vesiculosus </i>

Relevance. In conditions of intensive animal husbandry, it is important to normalize the immune and antioxidant systems of the animal organism. Seaweeds can be used to increase the natural resistance of the animal organism to adverse environmental factors at an early stage of ontogenesis in the post-milk growing period.Methods. The research was conducted at the Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst , on two groups of lambs with analogues in live weight and age (n = 8). Lambs of the experimental group received 3 g of brown algae F. Vesiculosus in addition to the diet. The duration of the experiment was 27 days with the study of the aftereffect after 28 days with individual weighing of lambs.Results. The live weight of lambs whose diet used brown algae was 14 kg, which is 5.3% higher than in the control.According to microbiological indicators of blood, there was a higher level of bactericidal activity in lambs treated with brown algae, the difference was 28.33%. The content of TBА-active products in blood serum was higher in animals of the control group by 14.57%, the difference in TAWA in the blood of animals was 16.93% in favor of animals of the experimental group. The absolute increase in live weight in the experimental group 28 days after the experiment was 17.9 kg, which is 7.8% higher than in the control group.

Influence of the probiotic “Bioseven” on the intestinal biocenose of white rats

The use of probiotics in animal husbandry ensures the maintenance of homeostasis of the digestive tract and prevents the development of factor infections in young animals – colibacteriosis, dyspepsia, and others, promotes the growth of animals with high survival rates. The main advantages of probiotics over chemotherapeutic drugs and antibiotics are that they are harmless to animal organism and are ecologically clean. In connection with the great attention paid to probiotics as ecologically safe drugs, the study of biological properties and the selection of bacterial strains, the most promising in the probiotic sense, are currently being intensified. This is the direction of the selection of species-specific strains for the intestinal biocenosis of a specific animal species, which have high colonization and antagonistic properties. Therefore, the development of the scientific basis for the creation of new probiotic preparations gave an impetus to their improvement and continued research in this direction. The aim of the work was to study the effect of the probiotic “Bioseven” on the intestinal biocenosis of white rats depending on the dose. Objectives of the study: to establish the toxicity of the drug “Bioseven” on laboratory animals with a single dose (“acute toxicity”) according to microbiological indicators; to investigate the toxicity of “Bioseven” on laboratory animals with long-term administration (“subacute toxicity”) according to microbiological indicators. Research work was carried out during 2022 at the department of veterinary and sanitary examination, hygiene of animal husbandry products and pathanatomy named after J. S. Zagaevskii. The toxicological characterization of the probiotic drug “Bioseven” (study of subacute and acute toxicity) was carried out under the conditions of the State Research Control Institute of Veterinary Medicines and Feed Additives (Laboratory of Pharmacology and Toxicology), (Lviv), the manufacturer of the drug was PP “BTU-CENTER” Ladyzhyn, Vinnytsia region. On the 31st day of use of the drug “Bioseven” (a feed additive with probiotic action) in a therapeutic dose, microorganisms of the genus Lactobacillus were sown 0.3 lg CFU/g more from the large intestine of experimental rats, microorganisms of the genus Bifidobacterium were sown at 0.5 lg CFU/g more than in the control group. The number of Candida fungi decreased by 0.6 lg CFU/g, and the total number of Escherichia coli – 0.2 lg CFU/g. When using the “Bioseven” probiotic in a 5-fold therapeutic dose, 0.5 lg CFU/g more microorganisms of the Lactobacillus genus and 0.6 lg CFU/g more Bifidobacterium microorganisms were sown from the large intestine of experimental rats than in the control group. At the same time, the number of Candida fungi decreased by 0.7 lg CFU/g, and the total number of Escherichia coli to 0.4 lg CFU/g. Under the conditions of use of the drug “Bioseven” in a 10-fold therapeutic dose, microorganisms of the genus Lactobacillus were sown from the large intestine of experimental rats, 0.9 lg CFU/g more, microorganisms of the genus Bifidobacterium 1.2 lg CFU/g more than in the control the group At the same time, the number of Candida fungi decreased by 1.1 lg CFU/g, and the total number of Escherichia coli by 0.8 lg CFU/g. Prospects for further research consist in the study of the impact of the biotic drug “Bioseven” on indicators of metabolic processes and productive qualities of farm animals.

Saving and productivity of broiler chickens for feeding an optimal dose of zinc proteinate

On the basis of conducted scientific research, it was set that the use of inorganic salts of trace elements in animal and poultry feed leads to environmental pollution with heavy metals due to their low biological availability for the animal organism. Therefore, in recent years, a number of investigations have been conducted to study the effectiveness of using organic trace elements in animal and poultry feed. The article deals with the results of a scientific and economic experiment on the investigation of the influence of domestically produced zinc proteinate in combination with zinc sulfate on indicators of preservation, growth intensity and feed conversion in broiler chickens. Broiler chickens of the control group were fed with a complete ration balanced in terms of necessary nutrients, in accordance with the age periods of the growth of the poultry, with the addition of 50 g per ton of zinc sulfate. Then, the broiler chickens of the research groups were received the same compound feed, but with the addition of 50 g and 30 g per ton of zinc proteinate compound feed. The live weight of broiler chickens of the research groups was significantly higher than the control. At the age of one week, it tended to increase in the chickens of the 2nd and 3rd research groups, and at the age of 14, 21, 28, 35 and 42 days, the live weight of the chickens of the 3rd research group exceeded the live weight of the poultry of the control group, respectively , by 15.8 g (Р < 0.05); 37.0 (P < 0.01); 96.8 (P < 0.05); 115.9 (P < 0.01) and 177.7 g (P < 0.05), or by 4.7 %, 4.6, 7.6, 6.5 and 7.1 %, while the chickens of the 2nd research group at this time exceeded the control group chickens in terms of live weight, but were slightly behind the peers of the 3rd research group. The researched doses of zinc proteinate contributed to the increase in the preservation of poultry stock by 4–5 %, the average daily weight gain of broilers in the research groups compared to the control, respectively, by 5.1–7.2 %. The European index of efficiency of broiler chickens growing for zinc proteinate feeding was increased by 48.4 and 76.7 units compared to the control group which were fed with zinc sulfate and at the same time feed costs per 1 kg of growth was decreased by 2.4–3.2 %.

Comparative proteomics of snake and lizard venoms: Unveiling the molecular basis of venom diversity and adaptation

<p>Venom protein profiling is a method used to analyze the composition and structure of proteins in venom. Venom contains a variety of neurotoxins and enzymatic proteins, which have toxic effects on human and animal organisms. By identifying the proteins in the venom, we can better understand the toxic mechanism of the venom, and provide important references for biomedical research and clinical treatment. The basic steps of venom protein profiling include sample preparation, mass spectrometry and data analysis. Based on this, the protein component analysis was carried out and the results were summarized. The diversity of snake venom and lizard venom can be explained by their protein composition and structure. Their differences at the protein level determine their different functions and pathways of action in toxicology, while also providing important resources for biodiversity and drug development.</p>

Advances in selenium supplementation: From selenium-enriched yeast to potential selenium-enriched insects, and selenium nanoparticles.

Selenium (Se) is an essential micronutrient that plays an important role in animal and human development and physiological homoeostasis. This review surveys the role of Se in the environment, plants and animal bodies, and discusses data on Se biofortification with different sources of supplementation, from inorganic to organic forms, with special focus on Se-enriched yeast (Se-yeast). Although Se-yeast remains one of the main sources of organic Se, other emerging and innovative sources are reviewed, such as Se-enriched insects and Se-nanoparticles and their potential use in animal nutrition. Se-enriched insects are discussed as an option for supplying Se in organic form to livestock diets. Se-nanoparticles are also discussed, as they represent a more biocompatible and less toxic source of inorganic Se for animal organisms, compared to selenite and selenate. We also provide up to date information on the legal framework in the EU, USA, and Canada of Se that is contained in feed additives. From the scientific evidence available in the literature, it can be concluded that among the inorganic forms, sodium selenite is still one of the main options, whereas Se-yeast remains the primary organic form. However, other potential sources such as Se-enriched insects and Se-nanoparticles are being investigated as they could potentially combine a high bioavailability and reduced Se emissions in the environment.

The effect of feeding different doses of herbal supplements from Hungarian sainfoin on the growth and metabolism in the body of calves

There have been studied the influence of feeding plant raw material obtained from Hungarian sainfoin having an immunostimulating effect on the growth rate of dairy calves, as well as on morphological and biochemical blood parameters and natural resistance of the animal organism. Calves of the I and II experimental groups in addition to the main diet were fed a phyto supplement made of Hungarian sainfoin mixed with concentrates in doses of 150 and 300 g per head per day during 92 days. The use of herbal supplements in feeding calves ensured an increase in live weight in the animals of experimental groups by 11.0 kg (6.55 %) and by 11.7 kg (6.96 %) (p <0.05) over the period of scientific and economic experiment, compared with analogues of the control group. From the analysis of blood parameters, it should be noted that protein indicators were higher by 1.36 % (I experimental) and by 4.74 % (p < 0.01) (II experimental); there was an increase in hemoglobin at the end of the experiment - by 5.88 % (I experimental) and by 12.15 % (p ≤ 0.01) (II experienced). When determining the indicators of natural resistance, it was found that in calves of experimental groups I and II, the phagocytic activity of leukocytes was higher by 4.16 and 17.29 % (p ≤ 0.01), respectively, in comparison with control animals. Analysis of the data obtained showed that the most optimal dose for use in the diets of heifers is 300 g per head per day, fed to calves of the II experimental group. This is confirmed by the improvement of biochemical, morphological and immunological parameters of animal blood, as well as by higher values of their live weight gain.

Simulation of Magnetotargeting of Medicines Based on the Calculation of Permeability of Human Tissues by the Electromagnetic Field

Analysis of studies in the field of targeted delivery of drugs, genes and stem cells showed a low level of accuracy of both applied and practical research in this area. Sufficiently encouraging results were obtained with extracorporeal electromagnetic action on a pharmacological complex with a ferromagnetic nanoparticle. With this approach, it is rather difficult to implement the algorithm for introducing the drug into the topographic region (target organ), since in practice, approaches to the clinical application of drug transport technology, taking into account the physicochemical properties of human body tissues, have not been studied in detail. The available models represent various physical and mathematical approaches that do not take into account the bioelectrical and electrostatic properties of the tissues of the organisms of experimental animals and humans. The creation of algorithms and software simulation of this technology will allow calculating variable frequency variables for magnetotargeting in a human digital phantom, which will reduce time spent at the stage of pilot and clinical trials and in the future will form the applied part of the innovative technology. The article presents the methodology and results of multiphysics and mathematical modeling in the Sim4Life for Science, V7.0 package on the example of calculating the control parameters of the electromagnetic field of the region in the area of normal administration of drugs – the vessels of the forearm.

Time to consider animal data governance: perspectives from neuroscience.

Scientific research relies mainly on multimodal, multidimensional big data generated from both animal and human organisms as well as technical data. However, unlike human data that is increasingly regulated at national, regional and international levels, regulatory frameworks that can govern the sharing and reuse of non-human animal data are yet to be established. Whereas the legal and ethical principles that shape animal data generation in many countries and regions differ, the generated data are shared beyond boundaries without any governance mechanism. This paper, through perspectives from neuroscience, shows conceptually and empirically that there is a need for animal data governance that is informed by ethical concerns. There is a plurality of ethical views on the use of animals in scientific research that data governance mechanisms need to consider. Semi-structured interviews were used for data collection. Overall, 13 interviews with 12 participants (10 males and 2 females) were conducted. The interviews were transcribed and stored in NviVo 12 where they were thematically analyzed. The participants shared the view that it is time to consider animal data governance due to factors such as differences in regulations, differences in ethical principles, values and beliefs and data quality concerns. They also provided insights on possible approaches to governance. We therefore conclude that a procedural approach to data governance is needed: an approach that does not prescribe a particular ethical position but allows for a quick understanding of ethical concerns and debate about how different positions differ to facilitate cross-cultural and international collaboration.

Лактулозосодержащие кормовые добавки как фактор прижизненного формирования качественной и безопасной продукции

Purpose. Evaluation of various types of modern lactulose-containing additives and the effectiveness of their use in feeding farm animals as one of the options for decision the problem of in vivo formation of high-quality and safe livestock products for the healthy nutrition of the modern population. Discussion. The revolution in feed production made by the Soviet scientist Minenkov A.R. in the middle of the 20th century, namely the use of antibiotics as a feed additive to stimulate productivity, became a failure by the beginning of the 21st century. By this time, the negative consequences of prolonged use of antibiotics were revealed, which led to the active rejection of them in animal husbandry practice as productivity stimulants. They were replaced by pro- and prebiotic feed additives that have a healing effect on animal organism, thereby beneficially influencing their productive characteristics. Prebiotics in feed production are more convenient to use than probiotics, as they have a greater degree of unpretentiousness to storage conditions, but at the same time they are easier to obtain than live microorganisms. Lactulose is the most well-known and proven prebiotic in animal husbandry. Feed additives based on it in the body of animals have an identical mechanism of action based on enzymatic cleavage in the large intestine of this disaccharide by bifidobacteria and lactobacilli, which triggers a number of interrelated processes that have a beneficial physiological effect. The considered results of the use of some lactulose-containing feed additives on broiler chickens, Saanen goats, pigs and rabbits made it possible to conclude that for the above animal species there is a similar positive effect from the presence of new lactulose-based feed additives in the diet: intensification of growth and development of animals, as well as strengthening of resistance to adverse external factors, which directly affects the life-time formation of better and safer livestock products. Conclusion. Reviewed studies on the use of modern lactulose-based feed additives and the results obtained confirm their ability to have a beneficial effect on the growth, development and resistance to negative factors from outside the body of farm animals, such as broiler chickens, goats, pigs and rabbits. This, in turn, contributes to the lifetime formation of improved quantitative and qualitative characteristics of the resulting livestock raw materials.

The effects of soil intake on the growth performance, rumen microbial community and tissue mineral deposition of German Mutton Merino sheep

Soil ingestion by livestock is common in grazing ecosystems, but few studies have been conducted to assess its effect on the animal organism. The topic is worthy of attention because these potential effects are likely to be enriched in the food chain and interfere with animal and human health. In this study, we present an indoor feeding trial conducted based on a completely randomized design to comprehensively evaluate the effects of simulated soil ingestion during grazing on nutrient digestibility, rumen fermentation, and microflora, and mineral deposition in the organs and tissues of sheep. Eighteen Mutton Merino crossbred sheep (42.7 ± 2.34 kg) were randomly allotted to three treatments and fed diets containing 0% (Control), 5% (SOIL5), and 10% (SOIL10) for 62 d, including a 7-d metabolism trial. It was found that soil intake altered the rumen fermentation in sheep, as evidenced by a decrease in total volatile fatty acids (VFA) and acetate concentrations in rumen fluid of 50.6% and 51.3%, respectively (p < 0.01), with soil proportion in the diet increased from 0% to 10%. Soil ingestion also reduced the species richness of rumen bacteria, with the relative abundance of Bacteroidetes decreasing significantly (p < 0.01), while that of Firmicutes and Proteobacteria increased considerably (p < 0.05). In terms of mineral elements deposition, higher levels of iron (Fe) were detected in the spleen and liver, and a higher concentration of copper (Cu) and zinc (Zn) in the liver were found in sheep fed a diet containing 5% soil compared to the other two groups (p < 0.05). Moreover, the concentrations of lead (Pb) in the liver and kidney, and arsenic (As) in the heart were also clearly increased after ingestion of soil (p < 0.05). Our findings indicate that although soil intake had no significant effect on the growth performance of sheep, it altered ruminal fermentation and increased the risk of excessive Fe, Pb, and As in their organism. This study supplies a theoretical basis for risk assessment of soil ingestion in grazing livestock.

PRIME EDITING - a new method of editing genes

The Prime Editing method introduces the expected manipulations within a given genome with a Cas9-nicase and pegRNA structure and a reverse transcriptase, which is responsible for the synthesis of the segment, which is then incorporated into the edited strand. This technique is based on the previously discovered CRISPR/Cas9 method. It differs from CRISPR/Cas9 in the absence of double cracks within the DNA helix, which is due to its complex structure, including the presence of additional elements, i. e. the reverse transcriptase and the matrix within the pegRNA. PE is used to modify the DNA double helix. The work deals mainly with the creation and improvement as well as testing of the modern Prime Editing method. Information on the structure and functioning of the system is provided, as well as the research carried out so far with the use of PE, carried out within the genomes of cells derived from plant, animal, and human organisms, is described. The paper also contains information on the potential benefits and hopes related to the use of this innovative method.

Review of the Biological Activity of Rutin: Antidiabetic, Antioxidant, Anti-inflammatory and Antitumor

Various parts of plants have long been widely used for the treatment and prevention of diseases. They are a valuable source of biologically active compounds, especially they are rich in polyphenolic structures. Rutin belongs to the group of some of the most common flavonoids in plant objects. Animal organisms do not have enzyme systems for its synthesis, so it enters the human body only with plant food. Rutin is widely used as a component in herbal medicines, multivitamin preparations, in the cosmetology, food and chemical industries. This article discusses the biological activity of rutin, namely antidiabetic, antioxidant, anti-inflammatory and antitumor activity. The article summarizes the current data on the above-mentioned types of biological activity. It follows from the presented research results that rutin is a promising compound with multi-target activity for the treatment of various diseases. It can serve as a basis for the creation of new highly effective pharmacological molecules based on it.

Pet safety guidelines for pediatric transplant recipients.

Approximately 70% (90.5 million) of United States (US) households own at least one pet. Dogs are the most common, making up about 38% of all household pets, followed by cats, which make up 25%. Other pets such as fish, birds, reptiles, and small animals such as hamsters, gerbils, and rabbits are less common household members. Pets are often considered a part of the family and there are significant medical and psychosocial benefits to pet ownership; however, the possibility of disease transmission exists related to the type of animal and infectious organism, and specific human risk factors. Immunocompromised individuals may be at increased risk of serious illness from zoonotic infections. During the transplant evaluation and routinely posttransplant, the multidisciplinary team should inquire about pet ownership and animal exposures to guide on potential risks. This review discusses the most common diseases seen in various household pets including dogs, cats, birds, fish, and some farm animals. We will also present guidelines for pet safety and include strategies to decrease the risk of infection while supporting the benefits of pet ownership after transplant.

The extent of economic success of the sustainable agricultural development strategy in the desert environment

The agricultural sector suffers from several problems in the state of EL-Oued in southern Algeria at the farm level as a productive unit in the rural desert area due to human practices. Changing the way of dealing with the components of agricultural work brings many benefits in various fields. The implementation of the strategy of sustainable agricultural development achieves positive results on four axes: first, the economy ensures a higher income for the farmer with less and regulated expenditures; second, the crop is more productive and of quality; third, preserving the environment through less resource consumption with the ability to recycle agricultural waste, which reduces the environmental pollution of soil and water; fourth, biodiversity of animal and plant organisms. All axes are in the interest of man as an activist, consumer, and living being who influences and is influenced by the environment in which he lives.

Growth rate of red fox puppies of Vyatka fire type

Purpose: assessment of the dynamics of the growth of the red fox in the process of its ontogenetic development.Materials and methods. The article describes and analyzes the body weight and linear body measurements of females and males of the red fox of the Ognivka Vyatskaya type in postnatal ontogenesis. The research was carried out in the Vyatka farm (Kirov region). The animals were kept in the same conditions, fed in accordance with age and physiological condition. Linear measurements and weighting were performed at 3, 4, 5, 6 and 7 months of age. Growth indicators were fox body weight, body length, snout, head and tail, girth and height of the chest, snout width, length and girth of the front and hind legs.Results. As a result of the studies, periods of intensive puppy growth were identified. The maximum increase in body weight and linear measurements is observed from 3 to 4 months of age, when intensive growth and development of the animal organism is observed. Throughout the study, the coefficient of body weight variation (CV) was maximal compared to linear body measurements and ranged from 6% in 3-month-old puppies to 17% in 6-month-old animals, which makes it possible to consider body weight as an individual indicator of puppy development. All linear measurements at all study timelines had a coefficient of variation (CV) below 10% (ranged from 1 to 8%), that is, one can judge the weak degree of variability of the studied features. Also, during all study periods, sexual dimorphism is visible, males significantly exceeded the size and body weight of females in all studied parameters (p &lt; 0.005).Conclusion. The dynamics of the growth and development of red fox puppies of the Ognivka Vyatskaya type were revealed. Age-related changes in the exterior parameters of young red foxes have been studied.

Selection of Animal and Plant Test Organisms for the Integral Assessment of the Quality of Natural Fresh Water

Selection of Animal and Plant Test Organisms for the Integral Assessment of the Quality of Natural Fresh Water

Impairment of bile acid metabolism and altered composition by lead and copper in Bufo gargarizans tadpoles

Lead (Pb) and copper (Cu) are two common heavy metal contaminants in environments, and liver is recognized as one of the main target organs for toxicity of Pb and Cu in animal organisms. Bile acids play a critical role in regulating hepatic metabolic homeostasis by activating farnesoid X receptor (Fxr). However, there were few studies on the interactions between bile acids and liver pathology caused by heavy metals. In this work, the histopathological changes, targeted metabolome and transcriptome responses in the liver of Bufo gargarizans tadpoles to Pb and/or Cu were examined. We found that exposure to Pb and/or Cu altered the hepatic bile acid profile, resulting in increased hydrophobicity and toxicity of the bile acid pool. And the expression of genes involved in bile acid metabolism and their downstream signaling pathways in the liver were significantly altered by Pb and/or Cu exposure. The alteration of bile acid profiles and the expression of genes related to bile acid metabolism might induce oxidative stress and inflammation, ultimately inducing hepatocyte injury observed in the histological sections. To our knowledge, this is the first study to provide histological, biochemical, and molecular evidence for establishing the link between Pb and Cu exposure, disturbances in hepatic bile acid metabolism, and liver injury.

Iodine Biofortification of Dandelion Plants (Taraxacum officinale F.H. Wiggers Coll.) with the Use of Inorganic and Organic Iodine Compounds.

Iodine is a crucial microelement necessary for the proper functioning of human and animal organisms. Plant biofortification has been proposed as a method of improving the iodine status of the population. Recent studies in that field have revealed that iodine may also act as a beneficial element for higher plants. The aim of the work was to evaluate the efficiency of the uptake and accumulation of iodine in the plants of dandelion grown in a pot experiment. During cultivation, iodine was applied through fertigation in inorganic (KI, KIO3) and organic forms (5-iodosalicylic acid, 5-ISA; 3,5-diiodosalicylic acid, 3,5-diISA) at two concentrations (10 and 50 µM). The contents of total iodine and iodosalicylic acids, as well the plant biomass and antioxidant capacity of dandelion leaves and roots, were analyzed. The uptake of inorganic and organic forms by dandelion plants was confirmed with no negative effect on plant growth. The highest efficiency of improving iodine content in dandelion leaves and roots was noted for 50 µM KI. The applicability of iodosalicylates, especially 5-ISA, for plant biofortification purposes was confirmed, particularly as the increase in the iodine content after the application of 5-ISA was higher as compared to that with commonly used KIO3. The chemical analyses have revealed that iodosalicylates are endogenous compounds of dandelion plants.