Animal Medicine Research Articles

A Systematic Review of Complementary and Alternative Veterinary Medicine in Sport and Companion Animals: Electrotherapy.

Electrotherapy modalities are currently used in the treatment of animals, but the evidence base supporting their use has not yet been systematically reviewed. Cochrane guidelines, as adapted by the Swedish Agency for Health Technology Assessment and Assessment of Social Services, were followed for this systematic review. A literature search regarding all currently known electrotherapy modalities applied to horses, dogs, and cats was conducted for the years 1980-2020 using three databases: CABI, PubMed, and Web of Science Core Collection. Of the 5385 references found, 41 articles were included in the review: 13 papers on pulsed electromagnetic field therapy (PEMFT), 7 on neural electrical muscle stimulation (NEMS), 5 on transcutaneous electrical nerve stimulation (TENS), 4 on static magnets, 3 on interference, 2 each on percutaneous electrical neural stimulation (PENS), bioelectricity, and diathermy, and 1 each on micro-pulsed stimulation, capacitive coupled electrical stimulation, and microwave therapy. The literature per modality was limited in quantity (mean 3.7 papers). Half of the articles were assessed to have a high risk of bias (20 high, 7 moderate, and 14 low). The existing literature used a spectrum of indications and treatment parameters, which makes comparisons and drawing conclusions to support the use of these modalities in clinical practice challenging. The current scientific evidence is not sufficient to support the clinical effects of electrotherapies for any clinical indication in horses, dogs or cats. The selected suggestive results warrant further high-quality research on PEMFT, NEMS, TENS, and PENS.

Historical Development Of Ankara University Faculty Of Veterinary Medicine Animal Breeding And Health Sciences Specialization High School

Ankara Üniversitesi Veteriner Fakültesi Hayvan Yetiştiriciliği ve Sağlık Bilimleri Uzmanlık Yüksek Okulu, 1965 yılında Veteriner Fakültesi Dekanı Prof. Dr. Selahattin Gürtürk döneminde kurulmuştur. Eğitim-öğretim faaliyetlerine 1968 yılında başlanan Uzmanlık Yüksek Okulunda 14 yıl boyunca kesintisiz olarak lisansüstü öğrenci yetiştirilerek veteriner hekimliği mesleğinin gelişimine katkı sağlanmıştır. Yükseköğretim Kanunu’nun yürürlüğe girmesinden sonra lisansüstü eğitimde gerçekleştirilen yeni yapılanma ile birlikte lisansüstü eğitim yeni açılan Sağlık Bilimleri Enstitülerine devredilmiş, bu çerçevede 1982 yılında Uzmanlık Yüksek Okulunun işlevlerine son verilerek Okul kapatılmıştır. Çalışmada, veteriner hekimliği alanında Türkiye’de ilk kez lisansüstü düzeyde krediye dayalı eğitim veren ve tarihsel süreçte veteriner hekimliğe ilişkin bilim alanlarında uzmanlık diploması veren tek kurum haline gelen bu Okulun tarihsel gelişimi ele alınmıştır. Çalışmanın ana materyalini, Ankara Üniversitesi Veteriner Fakültesi Dekanlık Arşivinden sağlanan birinci el kaynaklar oluşturmuştur. Bu kaynaklar arasında kuruma ait orijinal belgeler, diplomalar, faaliyet raporları, rehberler, yönetmelikler, fotoğraflar, uzmanlık ve doktora tezleri bulunmaktadır. Bunun yanı sıra kuruma ait bilgi sahibi olan kişilerin arşivlerinden faydalanılmıştır. Çalışma, kitaplar ve süreli yayınlar gibi ikinci el kaynaklarla desteklenmiştir. Çalışmanın kurgusu “kuruluş ve yapılanma”, “eğitim-öğretim” ve “kapatılma” olmak üzere üç başlık altında düzenlenmiştir. Okulun yapılanması, görev yapmış olan müdürler ve yönetim kurulu üyeleri, lisansüstü eğitim-öğretimin durumu, ders içerikleri, uzmanlık ve doktora eğitimi veren bilim dalları ve öğretim elemanları belirlenmiş, Okuldan mezun olan kişilerden 153’ünün isim ve tez bilgilerine ulaşılmıştır. Elde edilen veriler kronolojik sıra ile yazıya aktarılarak tarih metodolojisi içerisinde değerlendirilmiştir. Sonuç olarak, Ankara Üniversitesi Veteriner Fakültesi Hayvan Yetiştiriciliği ve Sağlık Bilimleri Uzmanlık Yüksek Okulunun tarihsel gelişimine ilişkin ulaşılabilen kaynaklardan sağlanan bilgilerle özelde veteriner hekimliği tarihi çalışmalarına genelde ise kurum tarihi çalışmalarına katkı sağlandığı ileri sürülebilir.

Mechanosynthesis of magnolol multicomponent crystalline solids for improved natural antibiotics and customizable release profiles

Antibiotic is one of the most important discoveries in human and animal medicine. However, the inefficient use of antibiotics has caused widespread and persistent contamination of ecosystems, setting off microbial resistance storms. Magnolol is a botanical antibiotic, but poor physicochemical properties result in low bioavailability. Increasing solubility of magnolol can help to reduce the doses of medications to patients, minimize bothersome side effects. In this work, three novel multicomponent crystalline solids were synthesized from magnolol and isomeric coformers by mechanochemistry. It was found that the multicomponent crystalline solids achieved the customizable release profile of magnolol by manipulating the substituent positions of the isomers and complexation. Antibacterial activity test showed that bioactivity on two bacteria was considerably improved by designed MGN multicomponent crystals. In addition, the coformers controlled the dissolution behavior and further stabilized the improvement according to the variable statistical analysis. In conclusion, the properties of antibiotic multicomponent solids can be manipulated through the coformers. This provides an effective strategy for managing the release of drugs to meet individual biological differences and diverse therapeutic needs.

Isolation and antibiotic sensitivity of Salmonella typhimurium isolates from poultry farms and slaughterhouses of Chitwan, Nepal

Nepalese poultry sector is progressing rapidly contributing around 4% of GDP with flourishing impact to provide sustainable and cheapest protein as source of human food. However, with the accelerating pathway, this industry is shed-back by outbreak of several infectious and zoonotic diseases impacting huge economic losses. Salmonella also cause infection in poultry birds and constitutes the largest reservoir of Salmonella organisms in nature. S. typhimurium is an unadopted serotype of Salmonella that may be transmitted to human, animals (poultry) and environment from contaminated food, feed and water. Use of antibiotics, good farm management practices and biosecurity are the treatment and control measures of this disease in poultry production. Development of antibiotic resistant strains of S. typhimurium thus not only pose considerable threat to clinicians, but is also of public health concern as these resistant strains after ingestion are capable of transferring resistance to other pathogens. The present study was undertaken to determine prevalence and antibiotics sensitivity of S. typhimurium strains isolated from domestic poultry from Chitwan, Nepal. Out of 79 farm and 23 chicken slaughterhouse sampling sites, the 107 (19.45%) out of 550 cultured specimens were confirmed for S. typhimurium. Amongst various antibiotics used for in vitro sensitivity testing, the two antibiotics: Colistin (Cl) and Enrofloxacin (Ex), were found to be 100% effective, whereas out of 22 antibiotics, 18 antibiotics showed weak to strong resistance pattern against S. typhimurium isolate. In the present study, Polymyxin and fluoroquinolone antibiotics were found to be most effective against S. typhimurium. Understanding the source of exposure to pathogens (especially resistant pathogens) provides some insight into antibiotic use and misuse in the region. Looking through the lens of One Health approach, the regulation of antibiotic use in animal medicine has direct implications for human health through the increased resistance of foodborne pathogens to vital antibiotic therapy. Thus, judicious use of antibiotics based on their sensitivity pattern should be practiced.

MRTCM: A comprehensive dataset for probabilistic risk assessment of metals and metalloids in traditional Chinese medicine

Traditional Chinese medicine (TCM) is still considered a global complementary or alternative medical system, but exogenous hazardous contaminants remain in TCM even after decocting. Besides, it is time-consuming to conduct a risk assessment of trace elements in TCMs with a non-automatic approach due to the wide variety of TCMs. Here, we present MRTCM, a cloud-computing infrastructure for automating the probabilistic risk assessment of metals and metalloids in TCM. MRTCM includes a consumption database and a pollutant database involving forty million rows of consumption data and fourteen types of TCM potentially toxic elements concentrations. The algorithm of probabilistic risk assessment was also packaged in MRTCM to assess the risks of eight elements with Monte Carlo simulation. The results demonstrated that 96.64% and 99.46% had no non-carcinogenic risk (hazard indices (HI) were < 1.0) for animal and herbal medicines consumers, respectively. After twenty years of exposure, less than 1% of the total carcinogenic risk (CRt) was > 10-4 for TCM consumers, indicating that they are at potential risk for carcinogenicity. Sensitivity analysis revealed that annual consumption and concentration were the main variables affecting the assessment results. Ultimately, a priority management list of TCMs was also generated, indicating that more attention should be paid to the non-carcinogenic risks of As, Mn, and Hg and the carcinogenic risks of As and Cr in Pheretima and Cr in Arcae Conch. In general, MRTCM could significantly enhance the efficiency of risk assessment in TCM and provide reasonable guidance for policymakers to optimize risk management.

Relationship between Blood Parameters and Outcome in Rescued Roe Deer.

Veterinary facility admissions of wild animals are increasing alongside the interest in wildlife diseases. To improve animal welfare, it is therefore important to increase veterinarians' knowledge of wild animal medicine and to improve the clinical and diagnostic procedures, especially in the case of patients affected by trauma or multiple traumas. Blood analysis can be a quick and minimally invasive way of gathering useful clinical information for adequate treatment and management, and, together with a good clinical examination, to help predict hospitalisation outcomes. Few papers have reported reference ranges for the haematological and biochemical parameters of roe deer. This study evaluates the haematological and biochemical parameters in traumatised roe deer in relation to animal hospitalisation outcomes. The study was carried out on a cohort of 98 roe deer divided into groups according to their age and hospitalisation outcome. For each animal, a panel of haematological and biochemical parameters was performed. Significant differences were found between unweaned (&lt;4 months old) groups in terms of MCV, MCH, CK, creatinine, total bilirubin, direct bilirubin, and indirect bilirubin, and between weaned (&gt;4 months old) groups for total bilirubin. Creatine kinase, creatinine, and bilirubin may be useful indicators to correlate with the severity of trauma and help predict prognosis.

Removal of Antibiotics from Aqueous Solutions by Bentonite Supported-Nanoscale Zero-Valent Iron

Nowadays, antibiotics are extensively used in human and animal medicine. The unconscious consumption of antibiotics and their mixing with water sources without adequate treatment have caused environmental problems. Several chemical and physical treatment methods applied for the removal of antibiotics from aqueous media offer alternative solutions by improving the treatment process and using new materials. As in many areas, nanomaterials are also used in treatment methods such as sorption, degradation, and oxidation. Among the nanomaterials, nano zero-valent iron (nZVI) is frequently preferred in treatment processes due to its abundance, cheapness, ease of application, and high removal performance. Besides its many advantages, nZVI agglomerates in the aqueous phase and exhibits unstable behaviors. This limits the surface area required for antibiotic removal and reduces the removal efficiency. In recent years nZVI has been used in water treatment technologies by being modified with many support materials such as bentonite to minimize particle agglomeration. Bentonite is clay-based support material that provides high removal efficiency and reduces the cost of treatment due to its abundance in nature. In this study, antibiotic pollution and treatment methods in water resources were reviewed and the antibiotic removal performances of bentonite-supported nVZI were investigated. In the studies, it was concluded that the antibiotic removal efficiency increased with the use of support materials.&#x0D;

Identification of Araceae in Pondok Buluh Training Forest, Simalungun Regency, North Sumatra Province

The Pondok Buluh Training Forest is located in Simalungun Regency, North Sumatra, which has a fairly high diversity of flora, one of which is a plant from the Araceae family. Araceae is a family of taro that has potential for ornamental plants, medicinal plants, food and animal feed, therefore it is important to conduct research on Araceae species in this location to determine the diversity and potential of Araceae. From the results of the research, it was found that there were 13 species of Araceae which were included in 6 genera, namely Aglaonema, Alocasia, Diffenbachia, Homalomena, Schismatoglottis and Syngonium. The species are Aglaonema crispum, Alocasia arifolia, Alocasia sp., Alocasia longiloba, Alocasia sanderiana, Alocasia scabriuscula, Dieffenbachia seguine, Diffenbachia amoena, Homalomena cordata schott, Homalomena pendula, Homalomena rubescens, Schismatoglottis calyptrata, and Syngonium podophyllum. The potential of Araceae plants is as a medicinal plant, ornamental plant, vegetable pesticide and animal feed.

Moisture content measurement model of whole package of Chinese medicinal materials based on microwave transmission technology

In view of the relatively low representativeness of manual sampling inspection, and long time-consuming in oven detection of moisture content, which delayed the subsequent production period, this paper proposed a scheme for rapid moisture quantitative detection for Chinese medicinal materials by microwave transmission technology, and took 8 different types of Chinese medicinal mate-rials as examples to analyze the feasibility and reliability of the scheme for the detection results of moisture content of the whole package of Chinese medicine. In the experiment, the least square method was used to establish the measurement model of microwave absorption rate-moisture content for each kind of medicinal material. The results showed that the microwave transmission measurement of moisture content achieved high-precision measurement of the moisture content of Schisandrae Chinensis Fructus, Ziziphi Spinosae Semen, Poria, Pheretima, Lilii Bulbus, Scutellariae Radix, and Galli Gigerii Endothelium Corneum. The measurement model of Ziziphi Spinosae Semen had the highest accuracy, and the R~2 and root mean square error of the validation set were 0.951 5 and 0.15%, respectively. At the same time, this study found that the microwave absorption intensity of animal medicines including Pheretima and Galli Gigerii Endothelium Corneum was much weaker than that of plant medicines such as Schisandrae Chinensis Fructus, but there was also a good linear relationship between microwave absorption and moisture content, which proved the universality of this method. However, this method was not suitable for Phellodendri Chinensis Cortex because its package contained iron wire. For the whole package of medicinal materials with uniform density and no metal inside, the microwave transmission technology for moisture content measurement can be used to detect the moisture content, which is an effective alternative method to detect the moisture content of medicinal materials.

Research on artificial substitutes of rare and endangered animal medicinal materials and industrialization

Rare and endangered animal medicine, a kind of important Chinese medicine, plays an irreplaceable role in the prevention and treatment of serious acute and chronic diseases and major diseases. Due to resource limitation and depletion and the increasing demand, rare and endangered medicinal animal resources have been drastically reduced or even extinct, which threatens the inheritance of traditional Chinese medicine, the integrity of clinical medicine, and the development of the traditional Chinese medicine industry. For the protection and sustainable utilization of Chinese medicinal resources, artificial substitutes came into being. However, there is still a lack of systematic research on the pharmacological effects and clinical applications of artificial substitutes and whether they can completely replace the original medicinal materials in clinical practice. Therefore, this study focuses on the status quo of rare and endangered animal medicinal materials and their artificial substitutes that have been marketed. To be specific, the two were compared and whether the artificial substitutes can completely replace the original medicinal materials was analyzed. In addition, constructive suggestions on the industrialization of artificial substitutes were put forward, which was expected to promote the sustainable development of Chinese medicinal resources.

Anticonvulsant Activity of Bombyx batryticatus and Analysis of Bioactive Extracts Based on UHPLC-Q-TOF MS/MS and Molecular Networking

Bombyx batryticatus (BB) is an anticonvulsant animal medicine in traditional Chinese medicine (TCM) and acts on the central nervous system. This research aimed to study the anticonvulsant effects of different polarity fractions of extracts from BB and to explore the components conferring anticonvulsant activity. Materials and methods: Crude extracts of BB at 20 g/kg were divided into different polarity fractions (petroleum ether, chloroform, ethyl acetate, water) and were administered to groups of mice before injecting pentylenetetrazol (PTZ) to induce convulsions. The animals were placed in chambers, and their behaviors were recorded for 30 min following the injection. Latency time, percent of protection, convulsion, convulsion rate, and convulsion score were determined for these mice. The compounds present in the different fractions were analyzed, and those from the fraction that conferred anticonvulsant activity were identified by high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF MS) and molecular networking (MN). The chloroform extract fractions (B-C) clearly increased the seizure latency time and protection percentage and decreased the convulsion percentage compared to the control group. The anticonvulsant effect of other extract fractions was not significant. Our study shows that the chloroform extract fractions (B-C) of BB have a significant anticonvulsant effect. We also identified 17 compounds including lumichrome, pheophorbide A, and episyringaresinol 4'-O-beta-d-glucopyranose that were found for the first time. The results of this study may lay the groundwork for studying compounds derived from Bombyx batryticatus and their anticonvulsant effect.

The application, value, and impact of outcomes research in animal health and veterinary medicine.

Outcomes research is a relatively recent field of study in animal health and veterinary medicine despite being well-established in human medicine. As the field of animal health is broad-ranging in terms of animal species, objectives, research methodologies, design, analysis, values, and outcomes, there is inherent versatility in the application and impact of the discipline of outcomes research to a variety of stakeholders. The major themes of outcomes relevant to the animal health industry have been distilled down to include, but are not limited to, health, production, economics, and marketing. An outcomes research approach considers an element of value along with an outcome of interest, setting it apart from traditional research approaches. Elements of value are determined by the stakeholders' use of products and/or services that meet or exceed functional, emotional, life-changing, and/or societal needs. Stakeholder perception of value depends on many factors such as the purpose of the animal (e.g., companion vs. food production) and the stakeholder's role (e.g., veterinarian, client, pet-owner, producer, consumer, government official, industry representative, policy holder). Key areas of application of outcomes research principles include comparative medicine, veterinary product development, and post-licensure evaluation of veterinary pharmaceuticals and/or biologics. Topics currently trending in human healthcare outcomes research, such as drug pricing, precision medicine, or the use of real-world evidence, offer novel and interesting perspectives for addressing themes common to the animal health sector. An approach that evaluates the benefits of practices and interventions to veterinary patients and society while maximizing outcomes is paramount to combating many current and future scientific challenges where feeding the world, caring for our aging companion animals, and implementing novel technologies in companion animal medicine and in production animal agriculture are at the forefront of our industry goals.

Systematic Review of Complementary and Alternative Veterinary Medicine in Sport and Companion Animals: Therapeutic Ultrasound.

To explore the scientific evidence for therapeutic ultrasound (TU), we conducted a systematic review of the literature on TU in dogs, horses, donkeys, and cats. In three major databases, relevant articles published in 1980-2020 were identified. The risk of bias in each article was evaluated. Twenty-four relevant articles on the effects of TU in dogs, nine in horses, two in donkeys, and one in cats were identified. TU usually involved 2-6 treatments weekly for up to 4 weeks. Articles on tendon, ligament, and bone healing, acute aseptic arthritis, osteoarthritis, paraparesis, hindquarter weakness, and back muscle pain were identified. In experimental bone lesions in dogs, there is moderate scientific evidence for enhanced healing. For the treatment of other musculoskeletal conditions, the scientific evidence is insufficient due to the high risk of bias. There is substantial evidence that continuous TU increases tissue temperature in muscles and tendons by up to 5 °C in healthy animals. For disorders in tendons, ligaments, muscles, and joints in sport and companion animals, there is insufficient evidence for the clinical effects of TU.

Systematic Review of Complementary and Alternative Veterinary Medicine in Sport and Companion Animals: Extracorporeal Shockwave Therapy.

Extracorporeal shockwave therapy (ECSWT) is increasingly used to treat different types of musculoskeletal conditions in sport and companion animals. To explore the scientific basis for the treatment, we conducted a systematic review of the literature on ECSWT used in horses, dogs, and cats. Relevant articles published in 1980-2020 were identified from three major databases. Each article was assessed for risk of bias. The review identified 27 relevant articles on the effects of ECSWT in horses, nine in dogs, but none in cats. Typically, ECSWT involved one to three treatment sessions at 1- to 3-week intervals. We identified studies on bone mass and bone healing, wound healing, navicular disease, ligament injury, desmitis, sesamoiditis, tendon injury, osteoarthritis, and short-term analgesic effects. Common to all indications was that the scientific evidence was very limited. For each separate indication, there were relatively few studies, many of which had methodological flaws. Where favorable results were reported, they were usually not replicated in independent studies. A few encouraging results were found. In sport and companion animals, the scientific evidence for clinical effects of ECSWT in horses, dogs, and cats is limited. For some applications, notably short-term pain relief, ligament ailments, and osteoarthritis, the results seem promising and warrant further exploration in high-quality studies.

Molecular detection of Loxodontofilaria spp. in Asian elephants (Elephas maximus) from elephant training camps in Thailand

Filarial infection is an important disease in human and animal medicine. Several filarial worms are of importance, especially nematodes in the Onchocercidae. The Asian elephant (Elephas maximus) is an endangered animal and is very important from several socio-economic and ecological aspects in Thailand. Various parasites can be found in elephants; however, data related to filarial infections in elephants is limited. The objective of this study was to detect filaria in the blood of Asian elephants in Thailand, based on a polymerase chain reaction (PCR) technique. Blood samples were collected from 208 Asian elephants and detected for filaria using PCR, targeting the region of the internal transcribed spacer 2 (ITS2), the cytochrome c oxidase subunit 1 (cox1), and the RNA polymerase II large subunit (rbp1). In total, 4.33% (9 out of 208) of the sampled elephants had Loxodontofilaria spp. DNA with 100% query coverage. In addition, the obtained cox1 and rbp1 sequences matched with Loxodontofilaria sp., Onchocerca sp., and Dirofilaria sp. There were no identified risk factors (sex, age, location, and packed cell volume) related to Loxodontofilaria infection in elephants. The analyses of the phylogeny of ITS2 sequences demonstrated that the Loxodotofilaria-positive sequences were closely related to Onchocerca dewittei japonica and Onchocerca dewittei dewittei with 100% query coverage. Notably, the concatenated phylogenetic trees of ITS2 and the cox1 and rbp1 genes were closely similar to Loxodontofilaria sp. To describe in detail the genomic DNA of Loxodontofilaria spp., other genes should be additionally studied using a more discriminatory technique, such as DNA barcoding or whole genome sequencing.

Computational Design of a Chimeric Vaccine against Plesiomonas shigelloides Using Pan-Genome and Reverse Vaccinology.

The swift emergence of antibiotic resistance (AR) in bacterial pathogens to make themselves adaptable to changing environments has become an alarming health issue. To prevent AR infection, many ways can be accomplished such as by decreasing the misuse of antibiotics in human and animal medicine. Among these AR bacterial species, Plesiomonas shigelloides is one of the etiological agents of intestinal infection in humans. It is a gram-negative rod-shaped bacterium that is highly resistant to several classes of antibiotics, and no licensed vaccine against the aforementioned pathogen is available. Hence, substantial efforts are required to screen protective antigens from the pathogen whole genome that can be subjected easily to experimental evaluations. Here, we employed a reverse vaccinology (RV) approach to design a multi-antigenic epitopes based vaccine against P. shigelloides. The complete genomes of P. shigelloides were retrieved from the National Center for Biotechnological Information (NCBI) that on average consist of 5226 proteins. The complete proteomes were subjected to different subtractive proteomics filters, and in the results of that analysis, out of total proteins, 2399 were revealed as non-redundant and 2827 as redundant proteins. The non-redundant proteins were further checked for subcellular localization analysis, in which three were localized in the extracellular matrix, eight were outer membrane, and 13 were found in the periplasmic membrane. All surface localized proteins were found to be virulent. Out of a total of 24 virulent proteins, three proteins (flagellar hook protein (FlgE), hypothetical protein, and TonB-dependent hemoglobin/transferrin/lactoferrin family receptor protein) were considered as potential vaccine targets and subjected to epitopes prediction. The predicted epitopes were further examined for antigenicity, toxicity, and solubility. A total of 10 epitopes were selected (GFKESRAEF, VQVPTEAGQ, KINENGVVV, ENKALSQET, QGYASANDE, RLNPTDSRW, TLDYRLNPT, RVTKKQSDK, GEREGKNRP, RDKKTNQPL). The selected epitopes were linked with each other via specific GPGPG linkers in order to design a multi-epitopes vaccine construct, and linked with cholera toxin B subunit adjuvant to make the designed vaccine construct more efficient in terms of antigenicity. The 3D structure of the vaccine construct was modeled ab initio as no appropriate template was available. Furthermore, molecular docking was carried out to check the interaction affinity of the designed vaccine with major histocompatibility complex (MHC-)I (PDB ID: 1L1Y), MHC-II (1KG0), and toll-like receptor 4 ((TLR-4) (PDB: 4G8A). Molecular dynamic simulation was applied to evaluate the dynamic behavior of vaccine-receptor complexes. Lastly, the binding free energies of the vaccine with receptors were estimated by using MMPB/GBSA methods. All of the aforementioned analyses concluded that the designed vaccine molecule as a good candidate to be used in experimental studies to disclose its immune protective efficacy in animal models.

First assessment of the knowledge, attitudes, and practices of health actors in Togo and Ivory Coast in regard to antibiotic resistance

Background and Aim: Bacterial resistance to antibiotics has now become a threat to global public health. This study aimed to assess the knowledge, attitudes, and practices of health actors in relation to antibiotic resistance (ABR) in two African countries. Materials and Methods: A cross-sectional and descriptive questionnaire study was conducted in Ivory Coast and Togo from August 2020 to July 2021. Actors were interviewed both in person 63% (n = 141) and remotely due to COVID-19 restrictions. Qualitative variables were described by frequencies and quantitative variables by the mean and associated standard deviation. The bivariate analysis was conducted through the Chi-square test and exact Fisher test with an acceptable risk of 5%. Results: The results showed that 88% and 50% of the actors from Togo and Ivory Coast, respectively, had a good knowledge of ABR even if most of the stakeholders had limited knowledge of antibiotics that are banned or of critical importance in human and animal medicine. More than 75% of the actors had good perceptions in regard to ABR and these were significantly related to their profession. As for the factors leading to an increase in ABR, the interviewees perceived self-medication in humans as the main contributing factor. Approximately 70% of the actors admitted to following inadequate practices in terms of the use and/or distribution of antibiotics, and 58% and 46% of them used preventive antibiotic therapy in Togo and Ivory Coast, respectively. Similarly, in the two countries, 39% and 69% of the actors, respectively, claimed that they do not systematically use the antibiogram, and 69% and 61% of drug distributors, also respectively, admitted to occasionally selling antibiotics without a prescription. Finally, more than 80% thought that the authorities in their country did not communicate sufficiently about ABR. Among the actions that could help to combat ABR, actors considered the strengthening of controls on the distribution of antibiotics and the education of nonprofessionals on the importance of antibiotic preservation as priorities. Conclusion: Although this first study had some limitations, that is, the low number of surveyed actors and non-standardized questionnaire used, it revealed that health actors in Togo and Ivory Coast have a good knowledge and perception in regard to antibiotics and ABR, but also follow inadequate practices.

Pain Management and Analgesics Used in Small Mammals during Post-Operative Period with an Emphasis on Metamizole (Dipyrone) as an Alternative Medication.

Metamizole (dipyrone) is a widely used non-opioid analgesic in both human and animal medicine. Metamizole's safety has been the topic of numerous opposing debates, given the fact that in certain countries metamizole is frequently used as an over-the-counter (OTC) medicine, while in others it is banned due to the risk of agranulocytosis. Further, small mammals such as rabbits, ferrets, rodents, and hedgehogs have become some of the most common pets present in veterinary practice, and each of these species has specific analgesic needs due to their anatomy and physiology. The key to providing appropriate medical care is in finding a substance that has minimal negative effects. In small mammals, analgesia is an important factor and, it happens frequently that, pain in these patients is not well managed. Post-operative pain management is an important topic in the welfare of animals. The objectives of this review, thus, were to provide a concise overview of analgesics that are used in the treatment of postoperative pain in small mammals (e.g., rabbits and rodents) and to highlight the importance of this product, metamizole, in veterinary medicine, as well as the potential of this substance as an alternative analgesic for the treatment of postoperative pain in small mammals.

ETHNOPHARMACY SUKU ANAK DALAM OF BUNGKU VILLAGE, BAJUBANG DISTRICT, BATANGHARI REGENCY, JAMBI PROVINCE IN 2021

Suku Anak Dalam (SAD), the one of ethnics in Indonesia precisely on Jambi Province still use various types of plants and animals part as medical substance. Traditional medicine among local ethnics has differences of each other. In addition, the depletion of forests as a source of life and modern culture make traditional medicine been forgotten. The aim of this study was determine the souce of natural products used by SAD for medical purposes. It was a descriptive study using purposive sampling and snow ball sampling to get as much data as possible about medicinal plants or animals used by Suku Anak Dalam, Bungku Village. The results showed that there were 17 plats and 4 animals used by SAD for medicine. The disease are often experienced by Suku Anak dalam are stomachache, dyspepsia, kidney desease, dengue, hyperlipidemia, vulnus, toothache, and ascariasis. The part of natural product frequently used include root, stem, leaves, and sap, which were commonly processed by boiling, mashing, eating, searing, sticking, and burning. Based on the results that has been done that were 17 plants and 4 animals from different genus and family used as traditional medicine with different part and processed.

Aquatic Environments as Hotspots of Transferable Low-Level Quinolone Resistance and Their Potential Contribution to High-Level Quinolone Resistance.

The disposal of antibiotics in the aquatic environment favors the selection of bacteria exhibiting antibiotic resistance mechanisms. Quinolones are bactericidal antimicrobials extensively used in both human and animal medicine. Some of the quinolone-resistance mechanisms are encoded by different bacterial genes, whereas others are the result of mutations in the enzymes on which those antibiotics act. The worldwide occurrence of quinolone resistance genes in aquatic environments has been widely reported, particularly in areas impacted by urban discharges. The most commonly reported quinolone resistance gene, qnr, encodes for the Qnr proteins that protect DNA gyrase and topoisomerase IV from quinolone activity. It is important to note that low-level resistance usually constitutes the first step in the development of high-level resistance, because bacteria carrying these genes have an adaptive advantage compared to the highly susceptible bacterial population in environments with low concentrations of this antimicrobial group. In addition, these genes can act additively with chromosomal mutations in the sequences of the target proteins of quinolones leading to high-level quinolone resistance. The occurrence of qnr genes in aquatic environments is most probably caused by the release of bacteria carrying these genes through anthropogenic pollution and maintained by the selective activity of antimicrobial residues discharged into these environments. This increase in the levels of quinolone resistance has consequences both in clinical settings and the wider aquatic environment, where there is an increased exposure risk to the general population, representing a significant threat to the efficacy of quinolone-based human and animal therapies. In this review the potential role of aquatic environments as reservoirs of the qnr genes, their activity in reducing the susceptibility to various quinolones, and the possible ways these genes contribute to the acquisition and spread of high-level resistance to quinolones will be discussed.