Animal Science Research Articles

320 Ninety-nine years of accomplishment by Canadian Animal Scientists

Abstract The earliest ancestor of the Canadian Journal of Animal Science traces back to the Western Canada Society of Agronomy which was established in 1918. The Western Canada Society of Animal Production was formed in 1925 and the Eastern Society was established in 1926. These were the first vestiges of the Canadian Society of Animal Production which arose as result of the amalgamation of the chapters in 1951. The Agricultural Institute of Canada established the journal “Scientific Agriculture” in 1921, which was renamed the Canadian Journal of Agricultural Science in 1953. In 1957, the journal was split into plant, soil and animal sections and the Canadian Journal of Animal Science (CJAS) became the official publication of the Canadian Society of Animal Science (CSAS). Selecting the top Canadian animal scientists over the last 99 yr is no easy task and is marked with biases and subjectivity. Time is a major consideration, as in general contributions become less visible with time, even though they may have formed the foundation of future advances. Expectations of society members has also evolved over time, with a focus on the establishment of animal husbandry practices and Canada’s livestock population from the 1920s to the 1960s, refinement in nutrition and animal breeding practices in the 1960s to the 1980s, developments in molecular biology and genomics in 1980s to the 2000s and in precision animal production, animal welfare and environmental sustainability from the 2000s to the present day. To identify the top scientists in Canada, the editors-in chief of CJAS sent out letters to every federal and university animal science department in Canada to nominate four individuals whose contributions were felt to be particularly noteworthy. This exercise clearly demonstrated that Canadian Animal Scientists have made significant contributions in all fields at a global level of recognition. This presentation will introduce you to a few of these individuals. There is no doubt that the contribution of all Canadian animal scientists has had a major role in the development and success of the Canadian livestock industry.

463 Cyber-Physical Systems with robots and AI for precision dairy farming

Abstract The emergence of precision livestock farming (PLF) offers a transformative lens through which to prioritize animal welfare. At the heart of this transformation lies the rumen, a complex microbial ecosystem within dairy cows responsible for converting feed into nutrients and impacting cow health, productivity, and environmental footprint. Despite its pivotal role, our understanding of the inner workings of the rumen remains limited due to a lack of real-time monitoring tools. My research leverages AI and robotics in PLF to address this gap, focusing on developing a closed-loop system for continuous, in-vivo rumen monitoring. This system employs cyber-physical systems (CPS) embedded with modular sensors and adaptive algorithms. These sensor modules, powered by AI-based data tracking, autonomously monitor rumen temperature, humidity, pressure, and methane concentration, enabling early detection of imbalances and prompt interventions to maintain optimal rumen health. A key innovation is the self-powered in-vivo robot capable of traversing the rumen’s stratified layers. Guided by AI-powered localization, this robot unlocks unparalleled access to new data, aiding in identifying specific regions associated with conditions like subacute ruminal acidosis (SARA) and methane production. This non-invasive approach eliminates the need for traditional cannulation, minimizing stress on the animal and opening new avenues for animal scientists. The impact of this research extends beyond individual cow health. By optimizing rumen function, we can improve feed efficiency, reduce methane emissions, and promote overall sustainability in dairy farming. Additionally, real-time data insights pave the way for precision nutrition, tailored interventions, and improved disease prevention, directly enhancing animal welfare. This novel approach to rumen monitoring represents a significant step towards realizing the full potential of PLF. By integrating AI and robotics, we can usher in a future where animal welfare and environmental sustainability go hand-in-hand, ultimately contributing to a more responsible and ethical dairy industry.

Assessment of livestock husbandry practices and production constraints among smallholder mixed crop-livestock production systems in the Majang zone, southwest Ethiopia

Livestock production plays a significant role in improving the livelihoods of poor smallholder farmer households in the Majang zone. However, despite its importance, there is limited information on husbandry practices and constraints on livestock production to design and implement effective interventions to maximize productivity. The aim of this study was to assess husbandry practices and major constraints on livestock production in the Majang zone, southwest Ethiopia. A semi-structured questionnaire was used to collect data from a randomly selected sample of 168 households (HHs) through a face-to-face interview from January to May 2021. The results revealed that mixed crop-livestock farming is the dominant livestock production system in the study area. The overall average herd size per household (HH) was 2.15 ± 0.11 cattle, 0.15 ± 0.01 sheep, 0.31 ± 0.09 goats, 0.10 ± 0.02 donkey, 0.04 ± 0.00 mule, 0.13 ± 0.02 horses and 0.24 ± 0.02 chickens. Overall herd size did not differ significantly by AEZ except for sheep (p < 0.05). The primary reason for keeping cattle, goats, sheep, equines and poultry were milk production (27.4 %), meat for home consumption (33.9 %), cash income (33.3 %), transportation (85.1 %), and meat for home consumption (72 %), respectively. In the dry and wet seasons, natural pasture, crop residues, indigenous fodder trees and shrubs (IFTSs), nonconventional feed resources (NCFRs), crop stubbles and improved forages were the main livestock feed resources. The majority (60.1 %) of respondents practiced free-grazing systems. Separate sheds, family dwellings, kraals, and open-walled sheds were the main housing systems used for animals. The majority (79.2 %) of respondents used rivers as a source of livestock drinking water. Trypanosomiasis (18.45 %), African horse sickness (66.1 %) and Newcastle disease (47.02 %) were the most important diseases of livestock. Diseases (35.7 %), lack of access to artificial insemination (35.7 %) and inadequate veterinary services (11.3 %) were identified as the top three constraints limiting production. It is recommended that constraints identified by farmers should be considered in developing and implementing effective interventions to improve livestock productivity and their contribution to poverty alleviation in the study area.

Culture of care: the question of animal agency in laboratory animal science.

A majority of the current debates in experimental animal science research focus to a large extent on the significance and implementation of the 3Rs principle according to Russell and Burch. In this context, not least due to the EU Directive 2010/63/EU, the concept of a culture of care has become more prevalent. Although animals are essential actors in the field of laboratory science, the discussion around animal agency, as well as the resulting consequences for laboratory animal science, is currently unconsidered. The purpose of this qualitative survey was to identify the perception and understanding of professional workers in laboratory animal science regarding the culture of care in general and aspects of animal agency in particular. Using a non-standardized qualitative survey method (topic-oriented, guideline-based expert interviews), persons involved in animal experimentation in different groups (management, science, regulation, and care) were interviewed. Overall, the results of the qualitative survey showed that animal agency plays a subordinate role in the question of a culture of care in animal research. Although not all groups explicitly applied the construct of animal agency or comparable terminology for this, there were links to the theoretical construct. Overall, the interviews showed a recognized network between humans and animals and that animals can interact dialogically in research. This is justified, for example, by the transfer of emotions from carers or scientists to animals. Nevertheless, a differentiated reflection of an animal's agency remains disregarded. The present qualitative survey approached the understanding of a culture of care among experts in the field of animal research. Animal agency does occur in the theoretical reception of the culture of care model. However, it is not conclusively established in everyday practice. Rather, the results lead to the assumption that strategies are being implemented to largely fade out animal agency.

Development and Validation of Serotype-Specific Blocking ELISA for the Detection of Anti-FMDV O/A/Asia1/SAT2 Antibodies.

Foot-and-mouth disease (FMD) is one of the most infectious viral transboundary diseases of livestock, which causes devastating global economic losses. Different enzyme-linked immunosorbent assays (ELISAs) are used for sero-surveillance of the foot-and-mouth disease virus (FMDV). However, more sensitive, accurate, and convenient ELISAs are still required to detect antibodies against FMDV serotypes. The primary goal of this study was to establish serotype-specific monoclonal antibody (mAb)-based blocking ELISAs (mAb-bELISAs) that would provide better performance characteristics or be equivalent in performance characteristics compared with a conventional polyclonal antibody (pAb)-based competitive ELISA (pAb-cELISA). Four mAb-bELISAs were developed using FMDV serotype-specific mAbs for the detection of anti-FMDV/O/A/Asia1/SAT2 antibodies. Using a 50% cut-off, all four mAb-bELISAs exhibited species-independent 99.74%, 98.01%, 96.59%, and 98.55% diagnostic specificity (DSp) and 98.93%, 98.25%, 100%, and 87.50% diagnostic sensitivity (DSe) for FMDV serotypes O, A, Asia1, and SAT2, respectively. In addition, a 100% DSe of serotypes O- and SAT2-specific mAb-bELISAs was observed for porcine sera when the cut-off was 30%. All mAb-bELISAs developed in this study displayed high repeatability/reproducibility without cross-reactivity. Finally, the diagnostic performance of mAb-bELISAs was found to be better than or equivalent to compared with pAb-cELISAs, suggesting that mAb-bELISAs can be used to replace existing pAb-ELISAs for the detection of antibodies against these four FMDV serotypes.

Simultaneous detection of enrofloxacin and chloramphenicol antibiotics using surface-enhanced Raman spectroscopy combined with triangular silver nanoplates

The emergence of antibiotic residues in the environment and food requires the development of sensitive and selective detection methods for monitoring these contaminants. In fact, many different types of antibiotics will be used simultaneously to prevent diseases in livestock and aquatic animals, causing antibiotic residues in food and the environment. Rapid and accurate simultaneous analysis of multiple antibiotics at low concentrations remains a major challenge in in the field of rapid detection. This study presents a sensitive and selective method for the simultaneous detection of two widely used antibiotics, enrofloxacin and chloramphenicol, by combining surface-enhanced Raman spectroscopy (SERS) with triangular silver nanoplates. Triangular silver nanoplates (TAgNPls) in the form of colloid were simply synthesized by chemical reduction method and then used as an effective SERS substrate, enhancing the Raman signals of the target antibiotics and enabling the detection of these antibiotics at trace levels. It was found that with a SERS substrate assembled from the above TAgNPls, the antibiotics enrofloxacin and chloramphenicol could be detected with detection limits of 2.6 µg/L and 4.3 µg/L, respectively. In addition, this SERS substrate also allows simultaneous detection of the above two antibiotics in one analytical sample with concentrations as low as 10 µg/L for both substances. The proposed method holds promise for addressing concerns related to antibiotic contamination in various environments and food sources, contributing to the advancement of analytical techniques for the trace detection of pharmaceutical residues.

Effects of Changing Weather Patterns on Household Food Availability in Bukiro Sub County Mbarara District

Changing weather patterns and their variables are already negatively impacting food security by damaging crops, reducing yields, and increasing the prevalence of pests and diseases. These pests and diseases contend with crops for sunlight, water, and nutrients, further diminishing agricultural yields and creating issues for crops that were previously unexposed to these threats. Climate-related disruptions in food distribution and transportation, both internationally and domestically, have a substantial impact on not just safety and quality but also access to food. Prolonged dry spells followed by heavy and stormy rains cause interruptions in food production and transportation, reducing farmers' ability to get their grains to market and potentially affecting food prices. This study assessed farmers’ perceptions of effects of changing weather patterns on food security and their coping strategies towards effects of weather changing patterns in smallholder households in Bukiro Sub County, Mbarara District. A sample of 100 smallholder farmers were randomly selected from four parishes of Bukiro sub county and studied using questionnaire and observations. Key informants Interviews were also conducted with nine technical workers and three opinion leaders. Software STAT 26 and Ms excel version 2016 were used to do data analysis. The study found out that changing weather patterns through its variables specifically heavy and stormy rains, late of set of rains, and prolonged dry spells had significant effects on food security. The perceived effects of changing weather patterns established were drying up of water sources (83.8%), decreased land for crop growing (82.4%), reduction in crop yields (77.7%), drying of pastures for animals (67.4%), drying of crops (58.1%), and occurrence of crop and livestock pests and diseases at (56.6%). Also, the perceived weather changing variabilities were late onset of rains (60.1%), prolonged dry spells were responded at 17.6%, and heavy and stormy rains 22.3%. From the study finding farmers clearly perceived that changing weather patterns had negative effects smallholders’ agricultural production systems impacting negatively on food security

414 Design thinking for engaged learning (DTEL) in animal science: Data-driven considerations to improve student and instructor experiences

414 Design thinking for engaged learning (DTEL) in animal science: Data-driven considerations to improve student and instructor experiences

A Review On Human Brucellosis: An Important Occupational Hazard

Human brucellosis is a re-emerging and neglected zoonotic disease caused by Brucella spp., with Brucella melitensis being the principal cause worldwide. Despite the disease's prevalence, serological surveillance is not routinely practiced, even in countries where Brucella is endemic. Although eradication efforts have been successful in many developed and developing countries, brucellosis continues to pose a significant health risk to both animals and humans. Transmission to humans occurs by direct or indirect contact with infected animals or their products. Veterinary professionals, especially those conducting per-vaginal examinations without proper protective gear, and animal handlers are particularly vulnerable to infection. The pathogenesis of brucellosis is complex, involving bacterial invasion of host cells, immune evasion, and the potential for chronic infection. In humans, brucellosis often becomes chronic, but in acute cases, it presents as undulant fever with nonspecific symptoms. To reduce morbidity and mortality, early diagnosis and timely antibiotic treatment are most important. For diagnosis of Brucella spp. reliable, rapid, sensitive, specific, easy-to-perform, and automated detection systems are urgently required. Currently, there is no safe and effective vaccine available for humans. Prevention relies on controlling the disease in domestic livestock, primarily through mass vaccination, effective heat treatment of dairy products, and hygienic precautions to minimize occupational exposure. Treatment requires prolonged antibiotic therapy with a combination of drugs. To control human brucellosis, awareness programs, safe livestock practices, and timely diagnosis are essential

411 Designing academic talent pipelines to meet employment needs of the animal science industry

411 Designing academic talent pipelines to meet employment needs of the animal science industry

MicroRNA expression profiling of ovine epithelial cells stimulated with the Staphylococcus aureus in vitro.

MicroRNAs (miRNAs) act as key gene expression regulators, influencing intracellular biological and pathological processes. They are of significant interest in animal genetics as potential biomarkers for animal selection and health. This study aimed to unravel the complex miRNA signature involved in mastitis in in vitro cell culture. For this purpose, we constructed a control and treatment model in ovarian mammary epithelial cells to analyze miRNA responses upon Staphylococcus aureus (S. aureus) stimulation. The high-throughput Illumina Small RNA protocol was employed, generating an average of 7.75million single-end reads per sample, totaling 46.54million reads. Standard bioinformatics analysis, including cleaning, filtering, miRNA quantification, and differential expression was performed using the miRbase database as a reference for ovine miRNAs. The results indicated differential expression of 63 miRNAs, including 33 up-regulated and 30 down-regulated compared to the control group. Notably, miR-10a, miR-10b, miR-21, and miR-99a displayed a significant differential expression (p ≤ 0.05) associated to signal transduction, transcriptional pathways, diseases of signal transduction by growth factor receptors and second messengers, MAPK signaling pathway, NF-κB pathway, TNFα, Toll Like Receptor 4 (TLR4) cascade, and breast cancer. This study contributes expanding miRNA databases, especially for sheep miRNAs, and identifies potential miRNA candidates for further study in biomarker identification for mastitis resistance and diagnosis.

Advancing Animal Health: A Web-Based Expert System Utilizing Forward Chaining for Disease Diagnosis

The increasing prevalence of animal diseases, along with the increasing need for animal products, highlights the urgent need for efficient diagnostic tools in veterinary medicine. The aim of this research is to create an expert system that uses a forward chaining algorithm to diagnose animal diseases. The forward chaining algorithm is a deductive reasoning approach that starts with existing facts and uses expert tree rules for hypotheses. This process continues until the desired goal is achieved or no additional conclusions can be drawn. Even though there are developments in expert systems, there are still shortcomings in implementing the forward chain for rapid and precise diagnosis of livestock diseases. This work aims to fill this gap by developing an expert system that improves the accuracy and efficiency of disease diagnosis in the livestock industry. A database of animal diseases and symptoms was created by observing and interacting directly with farmers. The system architecture is specifically intended to optimize data processing and user engagement, enabling rapid diagnosis and treatment recommendations. This test shows a level of accuracy and precision, thereby reducing the possibility of misdiagnosis. The capacity of expert systems to provide fast and reliable diagnoses has the potential to improve livestock health management, thereby helping farmers maintain animal welfare and productivity. The results of this work advance the field of veterinary diagnostics and propose other uses of expert systems in animal health management.

Effects of Inter-community Conflicts on Beef Cattle Production among Pastoral Communities of Marsabit County, Kenya

The focus of this research was to determine the effects of inter-pastoral communities conflicts on beef cattle production, to determine the effects of livestock diseases on beef cattle production among pastoral communities in Marsabit County. The study employed a descriptive survey research method. Our study's target population was made up of 1210 beef cattle keepers with a total population of 118,755 beef animals. Marsabit County was chosen at random using purposive sampling techniques. After stratification, 60 out of 200 registered farm groups was chosen at random. The researcher conducted a pilot study to determine the instrument's suitability. Data was statistically handled, manipulated and analyzed by Statistical Software for Social Sciences (SPSS) version 25. To achieve the objectives of the study, the researcher employed both descriptive and inferential statistics where correlation analysis, chi-square analysis and standard multiple regression analysis was used to explore the relationship between the variables as well as for making generalizations about the data. Diagnostic tests was checked to ascertain violation of assumptions. The study found out that these conflicts not only directly reduced cattle numbers but also instilled an atmosphere of fear and instability, discouraging investment and effort in cattle rearing. Proactive measures to mitigate inter-community conflicts, such as conflict resolution initiatives and improved security measures, are essential for safeguarding both livestock and human lives. The study recommends investing in conflict resolution measures and programs.

Quantification of wildlife visits to pig farms via camera traps in Japan

Infectious diseases in livestock not only cause significant economic losses but also affect food security. Although wildlife may be involved in these infectious diseases by serving as reservoirs, research has primarily focused on livestock and related species. Moreover, while these species represent a potential threat in wildlife-borne infectious diseases, comprehensive surveys of the presence of various species are limited. In this study, we aimed to quantitatively investigate the occurrence of various mammals and birds at three pig farms in Japan. We conducted camera trap surveys from October 15, 2020, to March 24, 2022, and determined the relative abundance index inside and outside the sanitary control zone. Wild boar (Sus scrofa), sika deer (Cervus nippon), and Japanese serow (Capricornis crispus) were photographed only outside the sanitary control zone. In contrast, small and medium-sized mammals, such as feral cats (Felis catus), raccoons (Procyon lotor), and rodent species (Muridae), and birds, such as the rufous oriental turtle dove (Streptopelia orientalis) and crows of the genus Corvus, were photographed both inside and outside the sanitary control zone. This comprehensive quantitative evidence suggests that various mammals and birds may be in indirect contact with livestock, highlighting the need to improve biosecurity at livestock farms. Moreover, while fences are effective against large mammals, they are inefficient against small to medium-sized mammals and birds. Therefore, the results of this study provide important insights into enhancing biosecurity on livestock farms by strengthening physical fencing, disinfecting clothing and equipment, and complying with standards of hygiene management.

Technical note: assessing GPS sensor accuracy using real-time kinematic device for livestock tracking.

The technical aspects of global positioning system (GPS) sensors have been improved substantially over the years, making them valuable and popular tools for livestock tracking. Using GPS sensors allows producers and researchers to locate grazing livestock, monitor their behavior and distribution, and gather referencing information about the health status of the animals and pastures. However, interpreting the behavior of grazing livestock, such as cattle, from GPS data can be difficult due to positioning inaccuracies. Without knowing the positioning accuracy range of GPS sensors, achieving high-level confidence in determining grazing densities and identifying abnormal livestock movement patterns is challenging. In this study, the positioning accuracy of 3 different types of commercial GPS sensors was assessed using a survey-grade real-time kinematics (RTK) device. We outlined the procedures and essential equations and compared the recorded locations from the GPS sensors with reference locations collected from the RTK device. The results demonstrated statistically significant differences (P < 0.05) in positioning accuracy between different types of GPS sensors. The circular error probable (CEP) at 95% probability levels (CEP95) of the 3 sensors ranged from 2.46 to 11.06 m. This accuracy varied among individual sensors within the same brand and across different brands, which underscores the importance of evaluating the positioning accuracy of GPS sensors in livestock tracking. This study provides significant insights for animal scientists, ecologists, and livestock producers engaged in GPS sensor-related research and practices.

Exploring the Life and Academic Experiences of International Students at the University of Jambi, Indonesia: A Case Study

The increasing number of international students in Indonesia's higher education sector urges institutions to improve the quality of their services, facilities, and education. The teaching and learning environment is critical, encompassing the effectiveness of instructional methods, classroom interactions, and the academic support systems provided by the university. Additionally, their socio-experiences, including integration into the campus community and interactions with peers, play a crucial role in their overall well-being and academic success. This study aims to explore the life and academic experiences of international students in undergraduate International Programs at the University of Jambi in Indonesia. This research employed a mixed-method approach, combining a questionnaire adapted from the Strategy Inventory for Language Learning (SILL) and in-depth interviews, with a total of 13 international students from international classrooms participating in the study. The research highlights significant challenges for international students at the University of Jambi, including inadequate classroom and laboratory facilities, poor lighting, and insufficient resources, particularly in Animal Sciences and Agriculture. Language barriers due to Indonesian being the primary medium of instruction further hinder learning. Students express high dissatisfaction with services such as insurance, health facilities, extracurricular activities, and counseling, though library services are well-regarded. Recommendations include adopting English for instruction, providing bilingual training for lecturers, and improving infrastructure and student services to enhance the overall academic experience for international students

Toward the Institutionalization of a One Health Agenda: What the World can Learn from Bangladesh

Abstract In 2005 and 2007, prompted by Nipah and avian influenza outbreaks, respectively, faculties at Chattogram Veterinary and Animal Sciences University (CVASU) in Bangladesh initiated a civil society network of researchers in animal and human health. This network led to the launch a year later of One Health Bangladesh (OHB), a platform with membership open to multi-disciplinary scientists, practitioners, and activists. Along with continued advocacy to advance One Health agenda, OHB partnered with government, UN agencies, and other stakeholders to develop, in 2012, a National One Health Strategic Framework and Action Plan. The National Plan was later endorsed by the Ministry of Health and Family Welfare (MoHFW), Ministry of Fisheries and Livestock (MoFL), and Ministry of Environment, Forest, and Climate Change (MoEFC) followed by its implementation which introduced a three-tier multi-sectoral coordination mechanism including the establishment of a One Heath Secretariat in 2016. In 2017, an operational budget for the One Health Secretariat (OHS) was allocated by the MoHFW and three mid-level government officers from three government agencies, and the Directorate General of Health Services (DGHS), Department of Livestock Services (DLS), and Bangladesh Forest Department (BFD) were seconded to the OHS to make the Secretariat functional. OHB and the OHS now work together to advance the institutionalization process and implement One Health activities including One Health capacity building, joint surveillance, outbreak investigation and intervention, advocacy in the form of arranging dialogs, seminars, workshops, and conferences, and promoting cross sectoral and transdisciplinary collaboration, networking, and partnership nationally and internationally. Information © The Authors 2024

Design and Application of an Evaluation Tool to Assess World Organization for Animal Health Competencies for Graduating (Day 1) Veterinarians.

Graduating competent veterinarians with the appropriate knowledge and skills to support and strengthen their country's National Veterinary Services is a key priority for veterinary institutions globally. The World Organisation for Animal Health (WOAH) developed a set of Day 1 Competencies that should be expected of every veterinary graduate. Veterinary schools need to be able to assess the coverage of these competencies in their curriculum and determine the level of proficiency of their graduates. This article describes the iterative design and development process used to create a semi-quantitative, competency-based assessment survey. The Evaluation Tool for WOAH Day 1 Graduating Veterinarian Competencies is used as part of a stepwise process to systematically assess a veterinary curriculum regarding these competencies. This tool was developed and tested at the University of Gondar College of Veterinary Medicine and Animal Sciences in Ethiopia. The Evaluation Tool was successful in systematically collecting, measuring, and analyzing the perceptions of faculty, senior veterinary students, recent graduates, and external stakeholders about the level of proficiency of graduates in all 19 WOAH Day 1 Competencies. It was specifically designed to be used in conjunction with curriculum mapping to provide a full picture of how effectively these competencies were taught and identify gaps that needed to be addressed. This tool, the supporting resources, and the methodology are now globally accessible to all veterinary institutions, enabling them to revise and update their curricula and, ultimately, improve the training of the future veterinary workforce to support veterinary services in their respective countries.

Diversity of bartonellae in mites (Acari: Mesostigmata: Macronyssidae and Spinturnicidae) of boreal forest bats: Association of host specificity of mites and habitat selection of hosts with vector potential.

Research into various bacterial pathogens that can be transmitted between different animals and may have zoonotic potential has led to the discovery of different strains of Bartonella sp. in bats and their associated ectoparasites. Despite their enormous species diversity, only a few studies have focussed on the detection of bacterial pathogens in insectivorous bats of boreal forests and their associated Macronyssidae and Spinturnicidae mites. We collected and molecularly analysed mite samples from forest-dwelling bat species distributed all along the boreal belt of the Palearctic, from Central Europe to Far East. Ectoparasitic mites were pooled for DNA extraction and DNA amplification polymerase chain reaction (PCRs) were conducted to detect the presence of various bacterial (Anaplasmataceae, Bartonella sp., Rickettsia sp., Mycoplasma sp.) and protozoal (Hepatozoon sp.) pathogens. Bartonella sp. DNA was detected in four different mite species (Macronyssidae: Steatonyssus periblepharus and Spinturnicidae: Spinturnix acuminata, Sp. myoti and Sp. mystacinus), with different prevalences of the targeted gene (gltA, 16-23S ribosomal RNA intergenic spacer and ftsZ). Larger pools (>5 samples pooled) were more likely to harbour Bartonella sp. DNA, than smaller ones. In addition, cave-dwelling bat hosts and host generalist mite species are more associated with Bartonella spp. presence. Spinturnicidae mites may transmit several distinct Bartonella strains, which cluster phylogenetically close to Bartonella species known to cause diseases in humans and livestock. Mites with ubiquitous presence may facilitate the long-term maintenance (and even local recurrence) of Bartonella-infestations inside local bat populations, thus acting as continuous reservoirs for Bartonella spp in bats.

A Low-power Environmental Supervision System Suitable for Livestock and Poultry Breeding

This paper is a kind of low power consumption environment suitable for livestock and poultry breeding regulation system, the system consists of livestock breeding related sensors, actuators, control equipment, LoRa gateway and control web parts, when the environment, carbon dioxide, carbon monoxide, methane, ammonia gas and hydrogen sulfide harmful gas concentration over the threshold will alarm and the system will take corresponding measures to ensure the production efficiency of animal husbandry and food safety, and reduce the incidence of livestock and poultry disease and environmental pollution.