Animal Cell Biotechnology Research Articles

Recommended Method for Chromosome Exploitation: RMCE-based Cassette-exchange Systems in Animal Cell Biotechnology

The availability of site-specific recombinases has revolutionized the rational construction of cell lines with predictable properties. Early efforts were directed to providing pre-characterized genomic loci with a single recombinase target site that served as an address for the integration of vectors carrying a compatible tag. Efficient procedures of this type had to await recombinases like PhiC31, which recombine attP and attB target sites in a one-way reaction - at least in the cellular environment of the higher eukaryotic cell. Still these procedures lead to the co-introduction of prokaryotic vector sequences that are known to cause epigenetic silencing. This review illuminates the actual status of the more advanced recombinase-mediated cassette exchange (RMCE) techniques that have been developed for the major members of site-specific recombinases (SR), Flp, Cre and PhiC31. In RMCE the genomic address consists of a set of heterospecific recombinase target (RT-) sites permitting the exchange of the intervening sequence for the gene of interest (GOI), as part of a similar cassette. This process locks the GOI in place and it is 'clean' in the sense that it does not co-introduce prokaryotic vector parts nor does it leave behind a selection marker.

Japanese animal cell biotechnology opens to the world

Japanese animal cell biotechnology opens to the world

Development of New Cell Lines for Animal Cell Biotechnology

Mammalian cell culture has been an important technique in laboratory-scale experimentation for many decades. Developments in large-scale culture have been due to the need to grow large numbers of cells to support the growth of viruses for vaccine production, and more recently, for growing hybridoma cells as a source of monoclonal antibody. Increasingly, however, pharmaceutical products such as hormones, enzymes, growth factors, and clotting factors are being produced from cell lines which have been manipulated by recombinant DNA techniques. It is clear, therefore, that the high cost of growing mammalian cells on a large scale does not necessarily prohibit their use for biotechnology, and indeed there is considerable evidence to suggest that animal cell biotechnology will continue to be a major growth area in the future.

Advances in animal cell biology and technology for bioprocesses

Van Wezel Lecture: "Contribution of Animal Cell Technologies to Healthcare", B.J.Montagnon animal cell lines regulatory issues in animal cell culture new techniques differentiation of animal cells in culture animal cell physiology development in serum-free media animal cell production systems downstream processing advances in product generation animal cell biotechnology in Europe.

Advances in Animal Cell Biology and Technology for Bioprocesses

Van Wezel Lecture: Contribution of Animal Cell Technologies to Healthcare, B.J.Montagnon animal cell lines regulatory issues in animal cell culture new techniques differentiation of animal cells in culture animal cell physiology development in serum-free media animal cell production systems downstream processing advances in product generation animal cell biotechnology in Europe.