And 3-aminopropyltriethoxysilane Research Articles

Selective extraction of Bactrocera oleae sexual pheromone from olive oil by dispersive magnetic microsolid phase extraction using a molecularly imprinted nanocomposite

Bactrocera oleae Gmelin, also known as olive fruit fly, is the main olive tree pest. It produces a severe effect not only on the productivity but also on the quality of the olive-related products. In fact, the oil obtained from infected olives has a lower antioxidant power. In addition, an increase of the oil acidity, peroxide index and UV-absorbance can also be observed. 1,7-dioxaspiro-[5,5]-undecane (DSU), is the main component of the sexual pheromone of this pest and may be used as marker of the pest incidence. In this context, the development of new methods able to detect the pheromones in several samples, including agri-food or environmental ones, is interesting. In this work, we synthesized a molecularly imprinted polymer (MIPS) layer over magnetic nanoparticles (Fe3O4@SiO2@MIP) for the selective recognition of DSU. They were prepared using DSU as template and 3-aminopropyltriethoxysilane (APTES) to associate the target analyte on the surface of the magnetic substrate and the later polymerization of ethylene glycol dimethacrylate (EGDMA) in presence of 2,2-azobisisobutyonnitrile (AIBN). The resulting Fe3O4@SiO2@MIP composite was characterized by different techniques. The maximum adsorption capacity of DSU on Fe3O4@SiO2@MIP in hexane was 32mg/g (5.3 times than that obtained for the non-imprinted composite). In addition, Fe3O4@SiO2@MIP showed a short equilibrium time (45min) and potential reusability. The combination of dispersive magnetic microsolid phase extraction and gas chromatography with mass spectrometric detection allows the determination of DSU in real samples at concentrations as low as 10μg/L with precision better than 7.5% (expressed as relative standard deviation). The relative recoveries are in the range between 95 and 99%, which indicates the potential of the methodology. Finally, it has been applied to real olive oil samples being the presence of the pest detected is some of them.

Photoluminescence of carbon dots from mesoporous silica

Photophysical properties of carbon dots were investigated under various excitation conditions and over a wide temperature region – from room to liquid helium temperatures. The carbon dots (CDs) were synthesized using mesoporous silica particles as a reactor and (3-aminopropyl)triethoxysilane (APTES) as a precursor. The photoluminescence spectra of CDs exhibit a strong dependence on the excitation wavelength and demonstrate a significant inhomogeneous broadening. Lowering sample temperature reveals the doublet structure of the spectra, which is associated with the vibronic structure of radiative transitions. The vibration energy ∼1200 cm−1 is close to the energy of CO stretching vibration. Long-lived phosphorescence of carbon dots with its decay time ∼0.2 s at T = 80 K was observed. The fluorescence and phosphorescence spectra are shown to be spectrally separated. The long-lived component of the emission was ascribed to optically forbidden triplet-singlet transitions. The value of the singlet-triplet splitting was found to be about 0.3 eV. Photo-induced polarization of the luminescence of carbon dots was revealed. The degree of the linear polarization is dependent on the wavelengths of both excitation and emitted light. The effect indicates a hidden anisotropy of optical dipole transitions in the dots and demonstrates the loss of the dipole orientation during the electron energy relaxation.

Gadolinium Nanoparticles Conjugated with Therapeutic Bifunctional Chelate as a Potential T1 Theranostic Magnetic Resonance Imaging Agent.

This work is directed toward the synthesis of two types of gadolinium oxide nanoparticles (Gd-oxide NPs), abbreviated as Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA, with diameters of 50-60 nm. The synthesis involves sequential coating of Gd-oxide NPs with tetraethyl orthosilicate (TEOS) and (3-aminopropyl) triethoxysilane (APTES), followed by functionalization of the aminopropylsilane group with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or 1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid conjugates of benzothiazoles (DO3A-BTA). Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA exhibit high water solubility and colloidal stability. The r1 relaxivities of both Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA are higher than those of the corresponding low-molecular-weight magnetic resonance imaging contrast agents (MRI CAs), and their r2/r1 ratios are close to 1, indicating that both can be used as potential T1 MRI CAs. Biodistribution studies demonstrated that Gd@SiO2-DO2A-BTA was excreted via both hepatobiliary and renal pathways. Gd@SiO2-DO2A-BTA exhibits a strong intracellular uptake property in a series of tumor cell lines, and has significant anticancer characteristics against cell lines such as SK-HEP-1, MDA-MB-231, HeLa, and Hep-3B.

Amino-terminated SiO2 aerogel towards highly-effective lead (II) adsorbent via the ambient drying process

This work demonstrated a low-cost sol-gel and ambient drying process towards amino-modified SiO2 (SiO2-NH2) aerogels powders possessing high potential in recovering lead (II) ions from waste water, using industrial waterglass and 3-aminopropyl triethoxysilane (APTES) as the starting materials. Effects of the APTES/SiO2 molar ratio on the chemical composition and pore structure of SiO2-NH2 aerogel were investigated, and the Pb2+ adsorption properties were examined using the batch method. Results showed that the SiO2-NH2 aerogels via the ambient drying route possessed the BET surface area of 400–470m2/g, pore volume of 1.4–1.8ml/g, and average pore diameter of 12–16nm. FTIR and chemical composition analysis confirmed the successful anchoring of the NH2 groups on the surface of SiO2 nanoparticles and the optimal APTES/SiO2 molar ratio was 0.21. The adsorption test showed that, at pH range of 4.0–6.0 and room temperature, the samples with the optimal APTES/SiO2 molar ratio of 0.21 exhibited relatively high adsorption capacity compared with the pure SiO2 aerogel and SiO2-NH2 aerogels with lower APTES/SiO2 molar ratio. An equilibrium adsorption time of 7h was observed, fitting well with the pseudo-second-order kinetic model, indicating that the chelating reaction between Pb2+ and anchored NH2 group was the rate-limiting step. The adsorption isotherm was well fitted by the Langmuir adsorption model, with the maximum adsorption capacity of 407.04mg/g. The rapid adsorption test simulating the filter membrane with SiO2-NH2 aerogel adsorbent indicated the removal percentage of higher than 80% after 5cycles.

Influence of functionalization type on controlled release of emodin from mesoporous silica

Emodin drug was introduced into the porous mesochannels of (γ-chloropropyl)triethoxysilicane (CPTES) and (3-aminopropyl)triethoxysilane (APTES) functionalized SBA-15 via a hydrothermal process. The pure, functionalized and drug loaded mesoporous materials were characterized by small angle X-ray powder diffraction, field scanning electron microscopy, high resolution transmission electron microscopy, nitrogen adsorption–desorption, Fourier transformed infrared spectra, and UV–visible spectrophotometry. The functionalization of mesoporous silica reduced the degree of crystallinity of the resulting material. High resolution transmission electron microscope images of the functionalized materials demonstrated the reservation of the hexagonal mesoporous structure of SBA-15. Pure, CPTES and APTES-functionalized mesoporous silica were employed as base materials for the controlled release of emodin drug. Drug loading results revealed that the loading capabilities largely depend on the specific surface area, and pore volume and pore diameter of the carrier matrix. Emodin drug release profiles were studied in phosphate buffered saline with pH 7.4, and outcomes specified that the drug release rate could be controlled by the surface CPTES and APTES-functionalized carrier matrices. Emodin loaded functionalized materials presented a lower release rate compared to that of the pure SBA-15. Emodin loaded APTES-functionalized SBA-15 presented the lowest release amount of 74.5 % even up to 60 h. These results propose that the functionalized mesoporous silica is a favorable drug carrier for accomplishing prolonged release time.

Fabrication of Thickness-Controllable Micropatterned Polyelectrolyte-Film/Nanoparticle Surfaces by Using the Plasma Oxidation Method.

We have demonstrated a novel way to form thickness-controllable polyelectrolyte-film/nanoparticle patterns by using a plasma etching technique to form, first, a patterned self-assembled monolayer surface, followed by layer-by-layer assembly of polyelectrolyte-films/nanoparticles. Octadecyltrimethoxysilane (ODS) and (3-aminopropyl)triethoxysilane (APTES) self-assembled monolayers (SAMs) were used for polyelectrolyte-film and nanoparticle patterning, respectively. The resolution of the proposed patterning method can easily reach approximately 2.5 μm. The height of the groove structure was tunable from approximately 2.5 to 150 nm. The suspended lipid membrane across the grooves was fabricated by incubating the patterned polyelectrolyte groove arrays in solutions of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) giant unilamellar vesicles (GUVs). The method demonstrated here reveals a new path to create patterned 2D or 3D structures.

Preparation of spherical mesoporous aminopropyl-functionalized MCM-41 and its application in polyamide thin film nanocomposite reverse osmosis membranes

Aminopropyl-functionalized MCM-41 (NH2-MCM-41) was synthesized by the one-pot hydrothermal method using tetraethoxysilane (TEOS) and (3-aminopropyl)triethoxysilane (APTES) as silica sources, cetyltrimethylammonium bromide as template, ethanol as a cosolvent, and sodium hydroxide as the alkali source. The synthesized nanoparticles were noticeably monodisperse with standard deviation of under 10% for the mean particle size. The specific surface area, pore size, pore volume and amino group density of the NH2-MCM-41 varied with the content of APTES in the initial solution. The NH2-MCM-41-polyamide thin film nanocomposite reverse osmosis (TFN RO) membranes were obtained by interfacial polymerization of trimesoyl chloride (TMC) and m-phenylene-diamine (MPD). The hydrophilicity of the TFN RO membranes was improved by the incorporation of NH2-MCM-41 nanoparticles. The water flux of 54 L m−2 h−1 at 1.6 MPa for NH2-MCM-41-polyamide TFN RO membrane was nearly 2.8 times that of the pure polyamide thin film composite (TFC) RO membrane and the solute rejection was 96.5% with a 0.1% w/v NH2-MCM-41 loading.

Preparation and characterization of polystyrene sulfonic acid-co-maleic acid copolymer modified silica nanoparticles

Two types of silanes, including 3-glycidoxypropyltrimethoxysilane (GPTMS) and 3-aminopropyltriethoxysilane (APTES), were grafted onto polystyrene sulfonic acid-co-maleic acid (PSSA_MA) first, followed by the grafting reaction to the silica surface. The modification of PSSA_MA onto silica was confirmed through FTIR (Fourier transform infrared spectrum), NMR (Nuclear magnetic resonance), and TGA (Thermogravimetric analysis). The grafting ratio of PSSA_MA via APTES with 1-ethyl-3-(3- dimethylaminopropyl)-carbodiimide (EDC) activation reached 18.0 %. As for GPTMS case, the grafting ratio reached 14.4 %, which was slightly lower than that of APTES case. The grafting mechanisms for both cases were elucidated. The epoxysilane was found to react with maleic acid groups on PSSA_MA only. On the other hand, the aminosilane not only interacted with sulfonic groups but also activated maleic acid groups via EDC. However, without the use of the coupling agent, the grafting degree of PSSA_MA was only 3 %, which signified the essential role of coupling agents in this organically modified silica. The average particle size of silica was around 200 nm with or without organic modification from transmission electron microscopy (TEM) and Zetasizer analysis. The APTES-modified samples showed the highest improvement in the ion adsorption capacity in all investigated cases.

Hierarchical emulsion based hybrid ceramics synthesized with different siloxane precursor and with embedded nickel nanoparticles

An emulsion-based synthesis route for the generation of organo-silica-based monolithic hybrid ceramics with a high content of different organic groups and embedded nickel nanoparticles is presented. By this route hybrid ceramics with a hierarchical pore size distribution and tailorable surface characteristics can be obtained. Mixtures of methyl polysiloxane (MK), tetraethyl orthosilicate (TEOS) and (3-aminopropyl)triethoxysilane (APTES) with varying ratios are selected as precursors, cross-linked, and subsequently pyrolyzed at 500 or 600 °C. In this way monolithic emulsion based hybrid ceramics with three different organo-functionalized siloxane precursor can be synthesized. Additionally, by adding the metal precursor NiCl2, metal-containing emulsion-based hybrid ceramics can be achieved. By adjusting the oil/water ratio and using high- or low internal phase emulsions (HIPEs or LIPEs), controlled micro-, meso-, and macropore distributions are obtained. After pyrolysis, materials achieve specific BET surface areas of up to 550 m2/g. Adjustable surface characteristics in terms of hydrophobicity and hydrophilicity of the material surface by thermal decomposition behavior of precursors is demonstrated. Nickel is incorporated through the formation of complexes with APTES, resulting in well-distributed metal nanoparticles with average particle sizes between 35 and 69 nm. The material properties are pivotal features for catalysis and gas separation applications.

Poly(butylene succinate-co-adipate) Green Composites with Enhanced Rigidity: Influences of Dimension and Surface Modification of Kenaf Fiber Reinforcement

Kenaf fibers (KFs) were utilized as reinforcements to prepare poly(butylene succinate-co-adipate) (PBSA) green composites. Untreated KFs of KF35 (large dimension) and KF120 (small dimension) imparted the nucleation effect for PBSA crystallization. KF120 exhibited superior nucleation efficiency compared with KF35. The tensile/flexural moduli of PBSA drastically increased after the addition of KF35 or KF120. Successful modifications of KF35 through NaOH(aq) and 3-aminopropyltriethoxysilane (APS) were confirmed. The APS-treated KF (KF35AS) exhibited enhanced interaction with the PBSA matrix compared with NaOH-treated (KF35A) and untreated KF35. KF35A and KF35AS also facilitated the nucleation of PBSA crystallization. The KF35AS-incorporated composites exhibited the highest tensile/flexural moduli among the different KF-added systems at identical loadings. The tensile and flexural moduli increased to 629% and 360% (40 wt % loading), respectively, compared with PBSA. The enhanced interfacial interaction betwee...

Effect of silane coupling agents with different non-hydrolytic groups on tensile modulus of composite PDMS crosslinked membranes

In this paper, four silane coupling agents with different non-hydrolytic groups were used to treat a kind of hydrophilic fumed nanosilica to investigate the influence of different silane coupling agents on the mechanical properties of PDMS composite matrix. The four coupling agents were ethyl triethoxysilane (ETES), octyl triethoxysilane (OTES), phenyl triethoxysilane (PTES), and 3-aminopropyl triethoxysilane (APTES) which exhibited the same hydrolytic groups. For each coupling agent/nanosilica system, six modified nanosilica samples were prepared at different addition contents of the coupling agent; six corresponding PDMS composite crosslinked membranes were also prepared. It was found that compared with pure PDMS membrane, the addition of modified nanosilica to PDMS increased the tensile modulus of the composite membranes. The miscibility strongly influenced the tensile modulus of the composite membranes when the HSP (Hansen solubility parameter) difference Ra was adopted to characterize the miscibility between the non-hydrolytic groups of the four coupling agents and the solvent (n-hexane). A better miscibility induced a larger tensile modulus. When the length of the non-hydrolytic groups was larger than the distance between two adjacent hydroxyl groups, the sheltered hydroxyl groups by the non-hydrolytic groups would be prevented to couple with other coupling agent molecules, thereby reducing the available hydroxyl groups. When all of the potentially available hydroxyl groups at the nanosilica surface reacted with the coupling agent molecules, the tensile modulus of the composite membranes would reach a maximum value. In addition, according to the results of equilibrium swelling in ethanol and water, it was found that a membrane with large tensile modulus possessed strong resistant capability to swelling. From the results of ethanol and water pervaporation experiments, connection between ideal ethanol/water permeation selectivity or ideal ethanol/water diffusion selectivity and the tensile modulus was also observed.

Removal of direct dyes from aqueous solution by oxidized starch cross-linked chitosan/silica hybrid membrane

In this research, chitosan/oxidized starch/silica (CS/OSR/Silica) hybrid membrane was prepared by using oxidized starch and 3-aminopropyltriethoxysilane (APTES) as cross-linking agents. The characterizations of the hybrid membrane were investigated by using attenuated total reflection (ATR) spectroscopy, scanning electron microscopy (SEM), thermogravimetry (TG) analysis and swelling measurement. The CS/OSR/Silica hybrid membrane exhibited the improved thermal stability and low degree of swelling in water. The adsorption properties of the CS/OSR/Silica hybrid membrane were studied by using two direct dyes (Blue 71 and Red 31). The results indicated the adsorption capacity of the CS/OSR/Silica hybrid membrane was found optimal at pH 9.82 and temperature 60°C for Blue 71 and Red 31. The adsorption kinetic data followed pseudo-second order kinetic model and the adsorption behavior of the two dyes on the hybrid membrane fitted well with the Freundlich model. The CS/OSR/Silica hybrid membrane can be used as an appropriate biosorbent for removal of direct dyes from colored wastewater.

Silanized humic substances act as hydrophobic modifiers of soil separates inducing formation of water-stable aggregates in soils

In this study we successfully enhanced surface activity of natural humic substances (HS) with respect to mineral surfaces by functionalization with organosilanes. Of particular importance was that modification was conducted in water. Humates from coal and peat and 3-aminopropyl-triethoxysilane (APTES) were used as starting materials. Atomic force microscopy showed silanized HS produced self-assembled adsorption monolayers at the water–solid interface. These adlayers caused an increase in the surface hydrophobicity with contact angles equaling 56°. Application of silanized HS for treatment of different soil compartments including mineral soil separates and soil aggregates revealed an efficacy with respect to both re-assembly of soil aggregates and restoration of their water stability. Furthermore, field trials on the treatments of native sod-podzolic soil with silanized HS indicated improvements in soil structure as compared to non-treated soil or treatment with the non-functionalized humate. Results demonstrating the use of silanized HS as ecologically-safe, nature-inspired soil conditioners were promising.

Dielectric properties of surface treated multi-walled carbon nanotube/epoxy thin film composites

In this study, MWCNTs were used as a filler for epoxy thin film composites, where the thin film was fabricated using the spin coating method. We investigated the dielectric properties of the MWCNT-filled epoxy composite, with filler loading in the range from 0 to 1.5 vol%. Additionally, the effect of various type of MWCNT filler treatments using polyoxyethylene octyl phenyl ether (Triton X-100), sodium dodecyl sulfate (SDS) and 3-aminopropyltriethoxy silane (AMPTES) on the dielectric properties of epoxy thin film composites were investigated. The results showed that the increment of MWCNT loading increased the dielectric properties of the composite system. Meanwhile, chemically treated MWCNTs, especially MWCNT treated with SDS, showed good dielectric properties. Furthermore, morphological study showed an improvement in the dispersion of filler throughout the epoxy matrix in the treated composite system compared to the untreated system. TEM images also showed that treated MWCNT filler increased wall thickness of nanotube.

Studying various mixtures of 3-aminopropyltriethoxysilane (APS) and tetraethylorthosilicate (TEOS) silanes on the corrosion resistance of mild steel and adhesion properties of epoxy coating

Different mixtures of tetraethylorthosilicate (TEOS) and 3-aminopropyltriethoxysilane (APS), i.e. 100/0, 70/30, 50/50, 30/70 and 0/100, were applied on mild steel samples. The morphology and composition of the silane coatings were investigated by field emission scanning electron microscopy (FE-SEM), energy dispersive spectroscopy (EDS) and contact angle analysis. Open circuit potential (OCP) and electrochemical impedance measurements were performed on the bare steel and silane coated samples by electrochemical impedance spectroscopy (EIS). Results showed that a mixture of 70/30 of TEOS/APS resulted in a more consecutive and compact film formation on the steel surface. It was shown that the mixture of TEOS/APS provided better corrosion protection properties than the single ones. The film composed of APS decreased the contact angle more than TEOS. An epoxy coating was applied on the surface of steel samples treated by various mixtures of silanes. Pull-off and salt spray tests were performed on these samples to investigate the effects of surface treatment on the adhesion and corrosion protection properties of the epoxy coating. It was found that the mixture of silanes significantly enhanced the adhesion and corrosion protection properties of the epoxy coating.

Effects of in‐situ functionalization of carbon nanotubes with bis(triethoxysilylpropyl) tetrasulfide (TESPT) and 3‐aminopropyltriethoxysilane (APTES) on properties of epoxidized natural rubber–carbon nanotube composites

Composites of carbon nanotubes (CNT) and epoxidized natural rubber (ENR) were prepared by in‐situ functionalization of CNT with two alternative silane coupling agents: bis(triethoxysilylpropyl) tetrasulfide (TESPT) and 3‐aminopropyltriethoxysilane (APTES). The reactions of ENR molecules with the functional groups on CNT surfaces and with the silane molecules were characterized by Fourier transform infrared. Furthermore, cross‐link density, relaxation behaviors, curing, mechanical, electrical, and morphological properties of pristine ENR and the ENR composites were investigated. Very low percolation thresholds, at CNT concentrations as low as 1 phr, were observed in the ENR–CNT and the ENR–CNT–TESPT composites. This might be attributed to improvements in the chemical linkages between ENR molecules and functional groups on CNT surfaces that led to a homogenous dispersion of CNTs in the ENR matrix, with loose CNT agglomerates. POLYM. ENG. SCI., 55:2500–2510, 2015. © 2015 Society of Plastics Engineers

Modified nano Fe2O3-epoxy composite with enhanced mechanical properties

Abstract Epoxy nanocomposite (ENC) reinforced with Fe2O3 nanoparticles (NPs) has been prepared. The surface properties of Fe2O3 NPs were turned using poly(N-vinyl-2-pyrrolidone) (PVP) and (3-aminopropyl) triethoxysilane (APTES) as modified agent. Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA) tests revealed that PVP and APTES have been successfully grafted to the surface of Fe2O3 NPs. The modified Fe2O3 NPs were monodisperse in epoxy matrix as determined using transmission electron microscopy (TEM). FTIR measurements for ENCs indicated that modified Fe2O3 NPs were covalently incorporated into epoxy networks. A disproportionate increase in mechanical properties of the obtained ENCs was observed with the increased modified Fe2O3 NPs loading. When 4 wt.% modified Fe2O3 NPs were introduced, the tensile strength was increased by 50.2% and the fracture toughness, which expressed as stress intensity factor (KIC), was significantly increased by 106% based on single-edge notch bend test.

Preparation of microencapsulated medium temperature phase change material of Tris(hydroxymethyl)methyl aminomethane@SiO2 with excellent cycling performance

Abstract Polyalcohol is one of the important medium temperature phase change materials (PCMs) for thermal energy storage, in which liquid-free solid–solid PCMs are more promising although sublimation can hardly be avoided when crystals transform to the “plastic phase”. We reported here the preparation of encapsulated Tris(hydroxymethyl)methyl aminomethane (Tris) with the SiO2 shell by sol–gel process. Through the hydrolysis and polycondensation of the bicomponent silicon precursors mixed with tetraethoxysilane (TEOS) and 3-aminopropyl triethoxysilane (APTS) of proper ratio, Tris@SiO2 microcapsules with excellent sealing property can be obtained, which prevent the leakage of Tris outside the capsules. Besides, silica encapsulation doubles the thermal conductivity of the PCMs to 0.478 W/(m K). The DSC measurement shows that the Tris@SiO2 capsule owns a reversible phase change in the range of 110–155 °C, which will stabilize at about 146 J/g during 70 times of thermal cycling. The Tris@SiO2 capsules have good potential for medium temperature thermal energy storage applications.

Impact of silica-coating on the microwave absorption properties of carbonyl iron powder

Microwave absorption properties, especially the band width and depth of reflection loss are highlighted as key measurement in studies of microwave absorber. In order to improve the band width and depth of reflection loss of carbonyl iron powder (CIP), we prepared SiO2 layers on the surface of CIP by using tetraethyl orthosilicate (TEOS) as a SiO2 source and 3-aminopropyl triethoxysilane (APTES) as a surface modifier. SiO2 layer was formed by the hydrolysis of TEOS. The results show that after treatment the CIP is covered by a 5–10nm coating layer. Contrast to uncoated samples, coated samples show improved absorption properties. The minimum of reflection loss is −38.8dB at 11GHz and the band width of reflection loss exceeding −10dB is from 8GHz to 14GHz.

Functionalization of Mesoporous SBA-15 with APTES by Co-Condensation and its Effect on Immobilization of <i>Candida</i> <i>rugosa</i> Lipase

Functionalized SBA-15 with mesoscopic pore channels was synthesized by co-condensation of tetraethoxysilane and (3-aminopropyl) triethoxysilane (APTES) via hydrothermal process. Small-angle X-ray powder diffraction, scanning electron microscopy and transmission electron microscopy were used to monitor the effect of surface functionalization on the structural and textural features of the SBA-15. The results suggested that the structural ordering of functionalized SBA-15, as well as pore diameters, pore volumes and surface areas, were decreased with increasing the APTES molar ratio. Candida rugosa lipase (CRL) was used as a model enzyme for studying the effect of amino-functionalized on loading amount and enzymatic activity. The effects of pH and temperature on catalytic hydrolysis of tributyrin by immobilized CRL were investigated. The results showed that immobilized CRL had a well adaptability in a wide pH and temperature region, and CRL immobilized on functionalized SBA-15 exhibited much higher enzymatic activity than free CRL, especially, 5 mol% APTES functionalized SBA-15 immobilized CRL displayed the highest activity.