A method is described for producing and measuring distortions of the pupil in the rabbit in vivo, using magnetic forces of the order of 40–125 mg wt acting radially, for periods less than 1 sec. The radial displacement of the pupillary margin of the iris depends largely upon the size of the pupil, varying from more than 2 mm in mydriasis to less than 0·2 mm in meiosis. This is an extreme range found in twelve animals employing a force of 60 mg. When the pupil is of an intermediate size some 4–6 mm in vertical diameter a variation of the displacements may be obtained on different occasions in the same animal. This variation is probably not due to the experimental arrangement because the factor to which it is most obviously related is the direction from which the particular pupil size is reached. If a pupil of such intermediate size is reached from a position of relative meiosis such as when the light stimulus is reduced, the distortion of the pupil obtained is always definitely greater than when the pupil of that size is reached from relative mydriasis. The significance of this finding is discussed and it is suggested that the mechanical state of the iris in the rabbit is related not merely to the size of the pupil but is also determined partly by the viscoelastic properties of the ancillary structures, stroma and blood vessels as well as by the contractile elements.
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