ABSTRACT Three full-scale Dou-Gong specimens at column tops are made according to the Qing Dynasty “code of engineering practice,” based on the prototype of one Dou and two Sheng Jiaomaye Dou-Gong of Jiayuguan tower. The three-dimensional load-displacement curve of Dou-Gong is obtained using three-dimensional static loading tests. Furthermore, the deformation characteristics, three-dimensional compression stiffness, and three-dimensional load transfer law of each layer of the Dou-Gong are analyzed. The test results indicate that the vertical failure characteristics of the Dou-Gong are the splitting of the Flat-Fang, and there is no obvious damage to the horizontal members until toppling. The three-dimensional load-displacement relationship curve of the Dou-Gong can be simplified to a two-fold line model, vertical stiffness of the Dou-Gong is significantly greater than the horizontal stiffness, and the horizontal stiffness in the beam direction is greater than the horizontal stiffness in the Fang direction. Using ABAQUS software, a comparative analysis of the difference in mechanical properties between the Dou-Gong at the column tops and the Dou-Gong between the columns is performed. The research results can provide a theoretical basis for the structural performance safety assessment and preventive protection of the Jiayuguan tower and similar Chinese ancient wooden structures.