Ancient Dog Research Articles

An ancient Alaskan dog’s DNA hints at an epic shared journey

An ancient Alaskan dog’s DNA hints at an epic shared journey

An early dog from southeast Alaska supports a coastal route for the first dog migration into the Americas.

The oldest confirmed remains of domestic dogs in North America are from mid-continent archaeological sites dated approximately 9900 calibrated years before present (cal BP). Although this date suggests that dogs may not have arrived alongside the first Native Americans, the timing and routes for the entrance of New World dogs remain uncertain. Here, we present a complete mitochondrial genome of a dog from southeast Alaska, dated to 10 150 ± 260 cal BP. We compared this high-coverage genome with data from modern dog breeds, historical Arctic dogs and American precontact dogs (PCDs) from before European arrival. Our analyses demonstrate that the ancient dog belongs to the PCD lineage, which diverged from Siberian dogs around 16 700 years ago. This timing roughly coincides with the minimum suggested date for the opening of the North Pacific coastal (NPC) route along the Cordilleran Ice Sheet and genetic evidence for the initial peopling of the Americas. This ancient southeast Alaskan dog occupies an early branching position within the PCD clade, indicating it represents a close relative of the earliest PCDs that were brought alongside people migrating from eastern Beringia southward along the NPC to the rest of the Americas. The stable isotope δ13C value of this early dog indicates a marine diet, different from the younger mid-continent PCDs' terrestrial diet. Although PCDs were largely replaced by modern European dog breeds, our results indicate that their population decline started approximately 2000 years BP, coinciding with the expansion of Inuit peoples, who are associated with traditional sled-dog culture. Our findings suggest that dogs formed part of the initial human habitation of the New World, and provide insights into their replacement by both Arctic and European lineages.

Integrative analysis of DNA, macroscopic remains and stable isotopes of dog coprolites to reconstruct community diet

Paleofeces or coprolites are often used to reconstruct diet at archaeological sites, usually using macroscopic analyses or targeted DNA amplification and sequencing. Here we present an integrative analysis of dog coprolites, combining macroscopic analyses, stable isotope measurements, and DNA shotgun sequencing to examine diet and health status. Dog coprolites used in this study were recovered from the Janey B. Goode and East Saint Louis archaeological sites, both of which are located in the American Bottom, an extensive Mississippi River floodplain in Southwestern Illinois. Based on the context of recovery, coprolites are assigned to the Late Woodland and Terminal Late Woodland periods (ca. 600–1050 AD). Given the scarcity of human remains from this time period, these dog coprolites can be useful as a proxy for understanding human diet during the Late Woodland period. We find that the Late Woodland dogs consumed a variety of fish as well as bird and plant taxa, possibly including maize, and also harbored intestinal parasites and pathogenic bacteria. By sequencing the fecal microbiome of the coprolites, we find some similarities to modern dog microbiomes, as well as specific taxa that can be used to discriminate between modern and ancient microbiomes, excluding soil contaminants. As dogs are often used as a surrogate to assess human diet, humans living with these dogs likely had a similar diet and were affected by similar parasites. These analyses, when integrated, show a more comprehensive view of ancient dog and human diet and health in the region during the initial expansion of maize agriculture than any individual method could alone.

Dog domestication and the dual dispersal of people and dogs into the Americas

Advances in the isolation and sequencing of ancient DNA have begun to reveal the population histories of both people and dogs. Over the last 10,000 y, the genetic signatures of ancient dog remains have been linked with known human dispersals in regions such as the Arctic and the remote Pacific. It is suspected, however, that this relationship has a much deeper antiquity, and that the tandem movement of people and dogs may have begun soon after the domestication of the dog from a gray wolf ancestor in the late Pleistocene. Here, by comparing population genetic results of humans and dogs from Siberia, Beringia, and North America, we show that there is a close correlation in the movement and divergences of their respective lineages. This evidence places constraints on when and where dog domestication took place. Most significantly, it suggests that dogs were domesticated in Siberia by ∼23,000 y ago, possibly while both people and wolves were isolated during the harsh climate of the Last Glacial Maximum. Dogs then accompanied the first people into the Americas and traveled with them as humans rapidly dispersed into the continent beginning ∼15,000 y ago.

Полиморфизм ДНК собак (Canis familiaris L.). I. Происхождение, распространение собак в свете молекулярно-биологических данных об их митохондриальных и ядерных геномах

Information is given on the size and organization of the mitochondrial and nuclear genomes of dogs, including information on polymorphisms of some loci used to clarify the phylogenetic relationships of wolves and the first dogs, including hypotheses about the places of domestication of ancient now extinct wolves and the dates of these events. It is noted that the introduction of molecular biological methods in archaeology has allowed to obtain the principally new data on ancient wolves and dogs. Based on mtDNA polymorphism and nuclear DNA polymorphism, migration routes of already domesticated dogs together with humans have been tracked. The previously existing points of view about the origin of the first dogs in Western Europe, as well as in East Asia, have been supplemented in recent years by assumptions about the appearance of the first proto-dogs in Siberia.

Прасобаки, собаки и их будущее

The possible scenarios of the transformation of ancient now extinct wolves into a proto-dogs and the domestication of the latter, which could occur repeatedly, often ending for various reasons, are considered. It is noted that only a few ancient dog breeds are similar to their wolf ancestors and the Siberian Husky stands out among them, especially given the fact that now there is more and more evidence that the ancestral home of dogs is Siberia. Attention is drawn to a certain amazing attitude of people in modern society not to dogs themselves, but to their reflection as part of our life, when the word "dog" is often used in everyday speech mainly with a negative connotation. The future of dogs is also predicted, assuming their total DNA registration, which can bring a lot of benefits to both the whole society and its individual members in the form of dog breeders, and to the dogs themselves, as a result of which homeless stray dogs will no longer appear, posing a serious threat to human health and life, and the urban environment, including park areas, will become cleaner.

Population Dynamics in Italian Canids between the Late Pleistocene and Bronze Age.

Dog domestication is still largely unresolved due to time-gaps in the sampling of regions. Ancient Italian canids are particularly understudied, currently represented by only a few specimens. In the present study, we sampled 27 canid remains from Northern Italy dated between the Late Pleistocene and Bronze Age to assess their genetic variability, and thus add context to dog domestication dynamics. They were targeted at four DNA fragments of the hypervariable region 1 of mitochondrial DNA. A total of 11 samples had good DNA preservation and were used for phylogenetic analyses. The dog samples were assigned to dog haplogroups A, C and D, and a Late Pleistocene wolf was set into wolf haplogroup 2. We present our data in the landscape of ancient and modern dog genetic variability, with a particular focus on the ancient Italian samples published thus far. Our results suggest there is high genetic variability within ancient Italian canids, where close relationships were evident between both a ~24,700 years old Italian canid, and Iberian and Bulgarian ancient dogs. These findings emphasize that disentangling dog domestication dynamics benefits from the analysis of specimens from Southern European regions.

Dog domestication was multifaceted

Ancient Dog Genomics Dogs were the first domesticated animal, likely originating from human-associated wolves, but their origin remains unclear. Bergstrom et al. sequenced 27 ancient dog genomes from multiple locations near to and corresponding in time to comparable human ancient DNA sites (see the Perspective by Pavlidis and Somel). By analyzing these genomes, along with other ancient and modern dog genomes, the authors found that dogs likely arose once from a now-extinct wolf population. They also found that at least five different dog populations ∼10,000 years before the present show replacement in Europe at later dates. Furthermore, some dog population genetics are similar to those of humans, whereas others differ, inferring a complex ancestral history for humanity's best friend. Science , this issue p. [557][1]; see also p. [522][2] [1]: /lookup/doi/10.1126/science.aba9572 [2]: /lookup/doi/10.1126/science.abe7823

Origins and genetic legacy of prehistoric dogs.

Dogs were the first domestic animal, but little is known about their population history and to what extent it was linked to humans. We sequenced 27 ancient dog genomes and found that all dogs share a common ancestry distinct from present-day wolves, with limited gene flow from wolves since domestication but substantial dog-to-wolf gene flow. By 11,000 years ago, at least five major ancestry lineages had diversified, demonstrating a deep genetic history of dogs during the Paleolithic. Coanalysis with human genomes reveals aspects of dog population history that mirror humans, including Levant-related ancestry in Africa and early agricultural Europe. Other aspects differ, including the impacts of steppe pastoralist expansions in West and East Eurasia and a near-complete turnover of Neolithic European dog ancestry.

Ancient dog diets on the Pacific Northwest Coast: zooarchaeological and stable isotope modelling evidence from Tseshaht territory and beyond

Domestic dogs are frequently encountered in Indigenous archaeological sites on the Northwest Coast of North America. Although dogs depended on human communities for care and provisioning, archaeologists lack information about the specific foods dogs consumed. Previous research has used stable isotope analysis of dog diets for insight into human subsistence (‘canine surrogacy’ model) and identified considerable use of marine resources. Here, we use zooarchaeological data to develop and apply a Bayesian mixing model (MixSIAR) to estimate dietary composition from 14 domestic dogs and 13 potential prey taxa from four archaeological sites (2,900–300 BP) in Tseshaht First Nation territory on western Vancouver Island, British Columbia, Canada. Two candidate models that best match zooarchaeological data indicate dogs predominantly consumed salmon and forage fish (35–65%), followed by nearshore fish (4–40%), and marine mammals (2–30%). We compared these isotopic data to dogs across the Northwest Coast, which indicated a pronounced marine diet for Tseshaht dogs and, presumably, their human providers. These results are broadly consistent with the canine surrogacy model as well as help illuminate human participation in pre-industrial marine food webs and the long-term role of fisheries in Indigenous economies and lifeways.

DOGS OF WAR, OR DOGS IN WAR? THE USE OF DOGS IN CLASSICAL GREEK WARFARE

In 1941, E. S. Forster wrote a short article, published in this journal, which compiled all of the instances he could identify in the ancient source material that described dogs being used in a military capacity. G. B. A. Fletcher, who had identified a few obscure references that Forster had not cited, responded to Forster's paper later that same year. The purpose of both papers was simply the compiling of a list, a purpose that had been inspired by Forster's interest in the French army's recruitment of dogs on the outbreak of the Second World War. The result was a thorough catalogue of known examples, showing the ancient dog being used for a variety of purposes such as patrol work or observation duties, or being used as combatants or despatch couriers. The primary aim, according to Forster was to ‘make a comparison with modern practice’ – that is, the French practice he had read about; the only exception for which he could find no ancient evidence was what he called ‘Red Cross’ work.

Dogs return to the show

Conservation The ancient dogs of Oceania include several lineages with Asian ancestry that have since evolved across the South Pacific for more than 3000 years. These include the Australian dingo, but also many less well known and mostly extinct varieties such as the New Zealand kuri. New Guinea singing dogs comprise a small, and highly inbred, population in captivity and are generally believed to be extinct in the wild. Surbakti et al. bring a ray of hope with their finding that populations of so-called highland wild dogs living at high altitudes are indeed true New Guinea singing dogs. Genomic analysis of samples collected from several dogs show that they are derived from the same lineage as the captive population but contain significantly higher levels of genetic diversity. This finding brings hope for both protection of the species in the wild and for invigorating the captive population. Proc. Natl. Acad. Sci. U.S.A. 10.1073/pnas.2007242117 (2020).

Ancient DNA Evidence from China Reveals the Expansion of Pacific Dogs.

The ancestral homeland of Australian dingoes and Pacific dogs is proposed to be in South China. However, the location and timing of their dispersal and relationship to dog domestication is unclear. Here, we sequenced 7,000- to 2,000-year-old complete mitochondrial DNA (mtDNA) genomes of 27 ancient canids (one gray wolf and 26 domestic dogs) from the Yellow River and Yangtze River basins (YYRB). These are the first complete ancient mtDNA of Chinese dogs from the cradle of early Chinese civilization. We found that most ancient dogs (18/26) belong to the haplogroup A1b lineage that is found in high frequency in present-day Australian dingoes and precolonial Pacific Island dogs but low frequency in present-day China. Particularly, a 7,000-year-old dog from the Tianluoshan site in Zhejiang province possesses a haplotype basal to the entire haplogroup A1b lineage. We propose that A1b lineage dogs were once widely distributed in the YYRB area. Following their dispersal to South China, and then into Southeast Asia, New Guinea and remote Oceania, they were largely replaced by dogs belonging to other lineages in the last 2,000 years in present-day China, especially North China.

Population structure and genetic history of Tibetan Terriers

BackgroundTibetan Terrier is a popular medium-sized companion dog breed. According to the history of the breed, the western population of Tibetan Terriers includes two lineages, Lamleh and Luneville. These two lineages derive from a small number of founder animals from the native Tibetan Terrier population, which were brought to Europe in the 1920s. For almost a century, the western population of Tibetan Terriers and the native population in Tibet were reproductively isolated. In this study, we analysed the structure of the western population of Tibetan Terriers, the original native population from Tibet and of different crosses between these two populations. We also examined the genetic relationships of Tibetan Terriers with other dog breeds, especially terriers and some Asian breeds, and the within-breed structure of both Tibetan Terrier populations.ResultsOur analyses were based on high-density single nucleotide polymorphism (SNP) array (Illumina HD Canine 170 K) and microsatellite (18 loci) genotypes of 64 Tibetan Terriers belonging to different populations and lineages. For the comparative analysis, we used 348 publicly available SNP array genotypes of dogs from other breeds. We found that the western population of Tibetan Terriers and the native Tibetan Terriers clustered together with other Asian dog breeds, whereas all other terrier breeds were grouped into a separate group. We were also able to differentiate the western Tibetan Terrier lineages (Lamleh and Luneville) from the native Tibetan Terrier population.ConclusionsOur results reveal the relationships between the western and native populations of Tibetan Terriers and support the hypothesis that Tibetan Terrier belongs to the group of ancient dog breeds of Asian origin, which are close to the ancestors of the modern dog that were involved in the early domestication process. Thus, we were able to reject the initial hypothesis that Tibetan Terriers belong to the group of terrier breeds. The existence of this native population of Tibetan Terriers at its original location represents an exceptional and valuable genetic resource.

Evidence for Early European Neolithic Dog Dispersal: New Data on Southeastern European Subfossil Dogs from the Prehistoric and Antiquity Ages.

The history of dog domestication is still under debate, but it is doubtless the process of an ancient partnership between dogs (Canis familiaris) and humans. Although data on ancient DNA for dog diversity are still incomplete, it is clear that several regional dog populations had formed in Eurasia up to the Holocene. During the Neolithic Revolution and the transition from hunter-gatherer to farmer societies, followed by civilization changes in the Antiquity period, the dog population structure also changed. This process was due to replacement with newly formed dog populations. In this study, we present for the first time mitochondrial data of ancient dog remains from the Early Neolithic (8000 years before present (BP)) to Late Antiquity (up to 3th century AD) from southeastern Europe (the Balkans). A total of 16 samples were analyzed, using the mitochondrial D-loop region (HVR1). The results show the presence of A (70%) and B (25%) clades throughout the Early and Late Neolithic Period. In order to clarify the position of our results within the ancient dog population in Eneolithic Eurasia, we performed phylogenetic analysis with the available genetic data sets. This data showed a similarity of the ancient Bulgarian dogs to Italian (A, B, and C clades) and Iberian (clades A and C) dogs’ populations. A clear border can be seen between southern European genetic dog structure, on the one hand, and on the other hand, central-western (clade C), eastern (clade D) and northern Europe (clades A and C). This corresponds to genetic data for European humans during the same period, without admixture between dog populations. Also, our data have shown the presence of clade B in ancient Eurasia. This is not unexpected, as the B haplogroup is widely distributed in extant Balkan dogs and wolves. The presence of this clade both in dogs and in wolves on the Balkans may be explained with hybridization events before the Neolithic period. The spreading of this clade across Europe, together with the A clade, is related to the possible dissemination of newly formed dog breeds from Ancient Greece, Thrace, and the Roman Empire.

Are spinal deformities in ancient dogs related to weight bearing? The importance of fluctuating asymmetry

AbstractThe article “A howl from the grave: Osteological analysis of 12th‐ to 15th‐century dogs from Santa Ana, Manila, Philippines, International Journal of Osteoarchaeology, 2018” DOI:10.1002/oa.2644 discusses two vertebral abnormalities of ancient dogs: flattening of the tip and lateral deviation of the dorsal spinous process and considers possible causal use of dogs as beasts of burden. This idea also has been suggested in previous reports. We question this view and evaluate such spinal abnormalities in a modern veterinary context, using computerized tomography (CT)–scanned pet dogs that were not carrying weights or pulling travois or sledge. We show that identical asymmetries and anatomical variations occur in CT images of modern dogs that have not been carrying weights or pulling sledges or travois. We suggest that these features can be normal variants, expressing fluctuating asymmetry. We emphasize that health care settings provide the procedural mechanics and the means to evaluate and distinguish among diagnostic possibilities. We plead for increased collaboration among experienced veterinary specialists and zooarchaeologists when aberrations of ancient osteological structures are encountered. This cooperation will lead to more informed interpretations and avoid misdiagnosis and unrealistic lifestyle interpretations.

Dog guides as witnesses with specific reference to Miles and Houellebecq

Since Wild Dog first crawled from the Wet Wild Woods and laid his head on Woman’s lap, he has helped man, not only to hunt and protect, but also as guide. A guide with enhanced senses in the physical world who could find a way across unmarked landscapes, a clever empathic being who could lead man to certain places or to specific individuals. No wonder then that the best-known ancient dog deities accompany humans as guides, often on their way to the afterlife. Dog guides—not to be confused with guide dogs—have remained a constant feature of the representation of dogs in literature, reflecting as much of the nature of these dogs as of the nature and needs of the humans they attend. In this way, the human-animal relationship also reveals how the solipsistic tendencies of human self-definition limits our capacity for being in the world. In the two contemporary novels that form the basis of my enquiry, La Possibilité d’une île (2005) by Michel Houellebecq and Op ’n dag, ’n hond (2016) by John Miles, the agency of dog guides introduces an intriguing element of distancing, reminding us that the self has meaning only in relation to another and that human concerns are not absolute.

Lineage losses for man's best friend

Domestication Dogs have been present in North America for at least 9000 years. To better understand how present-day breeds and populations reflect their introduction to the New World, Ni Leathlobhair et al. sequenced the mitochondrial and nuclear genomes of ancient dogs (see the Perspective by Goodman and Karlsson). The earliest New World dogs were not domesticated from North American wolves but likely originated from a Siberian ancestor. Furthermore, these lineages date back to a common ancestor that coincides with the first human migrations across Beringia. This lineage appears to have been mostly replaced by dogs introduced by Europeans, with the primary extant lineage remaining as a canine transmissible venereal tumor. Science , this issue p. [81][1]; see also p. [27][2] [1]: /lookup/doi/10.1126/science.aao4776 [2]: /lookup/doi/10.1126/science.aau1306

The evolutionary history of dogs in the Americas.

Dogs were present in the Americas before the arrival of European colonists, but the origin and fate of these precontact dogs are largely unknown. We sequenced 71 mitochondrial and 7 nuclear genomes from ancient North American and Siberian dogs from time frames spanning ~9000 years. Our analysis indicates that American dogs were not derived from North American wolves. Instead, American dogs form a monophyletic lineage that likely originated in Siberia and dispersed into the Americas alongside people. After the arrival of Europeans, native American dogs almost completely disappeared, leaving a minimal genetic legacy in modern dog populations. The closest detectable extant lineage to precontact American dogs is the canine transmissible venereal tumor, a contagious cancer clone derived from an individual dog that lived up to 8000 years ago.

Comparison of village dog and wolf genomes highlights the role of the neural crest in dog domestication

BackgroundDomesticated from gray wolves between 10 and 40 kya in Eurasia, dogs display a vast array of phenotypes that differ from their ancestors, yet mirror other domesticated animal species, a phenomenon known as the domestication syndrome. Here, we use signatures persisting in dog genomes to identify genes and pathways possibly altered by the selective pressures of domestication.ResultsWhole-genome SNP analyses of 43 globally distributed village dogs and 10 wolves differentiated signatures resulting from domestication rather than breed formation. We identified 246 candidate domestication regions containing 10.8 Mb of genome sequence and 429 genes. The regions share haplotypes with ancient dogs, suggesting that the detected signals are not the result of recent selection. Gene enrichments highlight numerous genes linked to neural crest and central nervous system development as well as neurological function. Read depth analysis suggests that copy number variation played a minor role in dog domestication.ConclusionsOur results identify genes that act early in embryogenesis and can confer phenotypes distinguishing domesticated dogs from wolves, such as tameness, smaller jaws, floppy ears, and diminished craniofacial development as the targets of selection during domestication. These differences reflect the phenotypes of the domestication syndrome, which can be explained by alterations in the migration or activity of neural crest cells during development. We propose that initial selection during early dog domestication was for behavior, a trait influenced by genes which act in the neural crest, which secondarily gave rise to the phenotypes of modern dogs.