Anatomical Preparations Research Articles

Influence of CAD/CAM Material and Preparation Design on the Long-term Fracture Resistance of Endocrowns Restoring Maxillary Premolars

Objective: To evaluate the effect of different occlusal preparation designs and CAD/CAM materials on the fracture resistance of maxillary premolars endcrowns. Materials and Methods: sixty-four endodontically treated upper first premolars were randomly divided into four groups according to ceramic materials (Vita Enamic and IPS emax CAD) and occlusal preparation designs (Anatomical and horizontal butt joint). After teeth preparation, the restorations were all made by CAD/CAM system (Cerec MCXL). Half of each group had undergone cyclic fatigue testing of 105 cycles with 50N loading force at a frequency of 0.5Hz in a mechatronic chewing simulator machine, and then all samples were loaded to fracture using a universal testing machine with a cross head speed of 0.5mm/min recording the fracture resistance values in N . The specimens were measured and statistically analyzed using using three-way analyses of variance (ANOVA), followed by serial two-way and one-way ANOVAs at each level of the study. P-values were adjusted for multiple comparisons using BENFORRONI correction and the significance level was set at P ? 0.05 for all tests. Results: Vita Enamic endocrowns showed higher fracture resistance values than IPS e max specimens. Conclusions: Vita Enamic endocrowns with anatomical preparations were found to be more favourable restoring endodontically treated maxillary premolars. KEYWORDSEndocrowns; All-Ceramic; Fracture; Cyclic loading; CAD/CAM

Effect of cup medialization on primary stability of press-fit acetabular cups

BackgroundAppropriate restoration of the native centre of rotation is of paramount importance in total hip arthroplasty. Reconstruction of the centre of rotation depends on reaming technique: conventional approaches require more cup medialization than anatomical preparations. To date, the influence of cup medialization on socket stability in cementless implants is still unknown. MethodsTen cadaveric hemipelvises were sequentially reamed using anatomical technique (only subchondral bone removal with restoration of the native centre of rotation) and conventional preparation (reaming to the lamina and medializing the cup). A biomechanical test was performed on the reconstructions. Implant motions were measured with digital image correlation while a cyclic load of increasing magnitude was applied. FindingsNo significant difference was measured between the two implantation techniques in terms of permanent cup migrations. The only significant difference was found for the cup inducible rotations, where the conventional technique was associated with larger rotations. InterpretationConventional reaming and cup medialization do not improve initial cup stability. Beyond the recently questioned concerns about medialization and hip biomechanics, this is another issue to bear in mind when reaming the acetabulum.

Autopsy or anatomical dissection: evidence of a craniotomy in a 17th\u2013eighteenth century burial site (Ravenna, Italy)

Surgical procedures undergone in life, autopsies and anatomical preparations can all leave clearly identifiable traces on human skeletal remains. Several studies on skeletons from archeological contexts have identified traces of these practices. However, the distinction between medical/forensic autopsy and anatomical dissections for scientific research can be challenging. We report the case of a middle-aged female skeleton from the cemetery of the church of San Biagio (Ravenna, Italy), dating back to the 17th–19th centuries, that shows signs of a complete craniotomy. In an attempt to clarify the reason for this practice, we analyzed all pathological and non-pathological markers on the skeleton. We carried out anthropological analyses and osteometric measurements to determine the biological profile and the cranial capacity of the individual. Paleopathological investigation and analyses of traumatic injury patterns were carried out using both a morphological and a microscopic approach. While we observed that the craniotomy was performed with a rip saw, we identified perimortem blunt force trauma to the frontal bone and an osteolytic lesion on the inner surface of the frontal bone. No other pathology was recognizable on the skeleton. Our differential diagnosis confidently proved that the craniotomy was due to an autoptsy procedure and was not the result of an anatomical dissection. We believe that, among other possible reasons, failed surgery could likely be the motive behind the ordering of the autopsy.

Descripción de la técnica de Rhoton modificada para la preparación de encéfalos en cadáveres y su práctica en el adiestramiento neuroquirúrgico en Chile

Introducción: Los avances de la microcirugía y la implementación de nuevas técnicas neuroquirúrgicas hacen necesario que los neurocirujanos adquieran un mayor conocimiento de la neuromicroanatomía. Esto puede lograrse de mejor forma con el trabajo en especímenes cadavéricos en el laboratorio. Su estudio se hace más comprensible con una buena técnica de preservación cadavérica que permita mantener los tejidos biológicos por más tiempo, con una buena consistencia y con la inyección de silicona de color que permita distinguir claramente las estructuras venosas de las arteriales simulando mejor las condiciones de la realidad. Materiales y Métodos: Se utilizó una mezcla de solución conservadora compuesta por fenol, alcohol etílico y formalina para preservar las cabezas cadavéricas. Después de un período de tres semanas se realizó la inyección del material donde se administró una mezcla de silicona de color azul al sistema venoso y rojo al arterial. Las etapas de este proceso se describen detalladamente. Resultados: Desde el año 2014, un total de tres cabe-zas y un miembro superior se han preparado con la técnica descrita con resultados satisfactorios. Con el uso de material fotográfico se ilustran sus resultados. Se mencionan además las distintas variables involucradas en la obtención de una buena inyección. Conclusión: La preparación cadavérica como la antes descrita es un trabajo laborioso, pero que permite la obtención de preparados anatómicos de buena calidad que posibilitan su uso por mucho tiempo y permite distinguir claramente el sistema arterial del venoso.

АДАПТИВНЫЕ ПРЕОБРАЗОВАНИЯ СВЯЗОЧНОГО АППАРАТА КОЛЕННОГО СУСТАВА У ЛИСИЦ В УСЛОВИЯХ ИЗМЕНЕННОЙ ФУНКЦИОНАЛЬНОЙ НАГРУЗКИ

The aim of the research is identification of complex structural changes of the fox’s knee joint ligaments to ensure its high biomechanical potencies. A promising approach for the study of adaptive and compensatory changes of the joint as a multicomponent biomechanical system is to evaluate the structural transformations of its ligaments under conditions of a new functional load. The research was carried out on the basis of the Department of Animal Anato-my and Histology named after Professor A. F. Klimov «Moscow state Academy of Veterinary Medicine and Biotech-nology – MBA named after K. I. Scryabin» and on the basis of JSC «Breeding animal farm «Saltykovsky». The arti-cle presents information about morphological transformations of the lateral and median ligaments of the knee joint that determine its reliable stability. Numerous structural changes of the fox ligaments during cage housing is re-flected, which decrease in the thickness of bundles of collagen fibers and their compositional density compared to the standard structure (in wild individuals). Morphological transformations that can significantly reduce the strength and elasticity resistance of the ligaments and the biomechanical potential of the joint are shown. 23 fox individuals, including 15 individuals of the cage housing and 8 representatives of the natural habitat were studied. The re-search used a comprehensive methodological approach, including anatomical preparation, light microscopy of histological sections, scanning electron microscopy, micromorphometry and statistical analysis of the obtained digi-tal data. According to the results of the study, it was found that fox individuals from natural biocenosis, have colla-gen structures of the lateral ligaments different in waving and packing density from ones of cage housing, which can contribute to ensuring their biomechanical domination.

Karakter Anatomi Daun sebagai Bentuk Adaptasi Tumbuhan Penyusun Zonasi Mangrove di Banggai Kepulauan, Provinsi Sulawesi Tengah

Mangrove is an essential ecosystem that are located in the transition area of fresh and sea water. Therefore, species that grow are typical species that are able to adapt to the saline environment. The aim of this research is to characterize leaf anatomical structure for every species that compose the communities found in each mangrove zonations, including their similarities and dissimilarities characteristic. Sampling of examined leaf was carried out in the mangrove areas of Peling Island and Bakalan Island by making plots in four sites of the mangrove ecosystem area. Methods for leaf anatomical preparations were carried out using the paraffin-tert-butanol and paradermal sections. Anatomical characters as an adaptation to saline habitat are found in all species of mangrove plants, including thick cuticle and epidermal cells, thick leaves and water-storage tissue (hypodermis). In addition, there is found the presence of cork warts and sclereid as characteristic of the genus Rhizophora. Based on quantitative character observations, the mesophyll (palisade to sponge) ratio range from 0.28 ± 0.04 μm to 1.19 ± 0.42 μm, leaf thickness ratio 289,41 ± 38,21 μm to 695,20 ± 97,50 μm and the highest stomata density is owned by Lumnitzera littorea

Lasers in Transurethral Enucleation of the Prostate-Do We Really Need Them.

The acronym EEP, coding for transurethral Endoscopic Enucleation of the Prostate, was introduced in 2016 by the European Association of Urology (EAU) guidelines panel on management of non-neurogenic male lower urinary tract symptoms (LUTS) and benign prostatic obstruction (BPO). Since then, a laser-based treatment, Holmium Laser Enucleation of the Prostate (HoLEP), and the current-based treatment of bipolar enucleation of the prostate (BipoLEP) are equally appreciated as valuable options for the management of benign prostatic obstruction (BPO). This was mainly inspired by the results of two meta-analyses on randomized controlled trials, comparing open prostatectomy with either Holmium Laser Enucleation of the Prostate (HoLEP) or bipolar enucleation of the prostate (BipoLEP). Prior to that, HoLEP was embraced as the only valid option for transurethral enucleation, although evidence for equivalence existed as early as 2006, but was not recognized due to a plethora of acronyms for bipolar energy-based treatments and practiced HoLEP-centrism. On the other hand, the academic discourse focused on different (other) laser approaches that came up, led by Thulium:Yttrium-Aluminum-Garnet (Tm:YAG) Vapoenucleation (ThuVEP) in 2009 and, finally, transurethral anatomical enucleation with Tm:YAG support (thulium laser enucleation of the prostate, ThuLEP) in 2010. Initially, the discourse on lasers focused on the different properties of lasers rather than technique or surgical anatomy, respectively. In and after 2016, the discussion ultimately moved towards surgical technique and accepting anatomical preparation as the common of all EEP techniques (AEEP). Since then, the unspoken question has been raised, whether lasers are still necessary to perform EEP in light of existing evidence, given the total cost of ownership (TCO) for these generators. This article weighs the current evidence and comes to the conclusion that no evidence of superiority of one modality over another exists with regard to any endpoint. Therefore, in the sense of critical importance, AEEP can be safely and effectively performed without laser technologies and without compromise.

Morphology of the Vascular Bodies of the Encephalon’s Ventricles of Cow (Bos Taurus Taurus)

Abstract The aim of the research was to establish the morphology of the vascular bodies of the encephalon ventricles of cow (Bos taurus taurus). Methods used: thin anatomical preparation, histological method of examination, transmission electron microscopy. Given the relationship, structure and general origin of the vascular bodies, they were divided into bodies III, IV and the lateral ventricles of the encephalon. This unit has topographical nature. Functionally, vascular bodies are an indivisible organ whose main function is the secretion of the cerebrospinal fluid, which maintains the constancy of the central nervous system internal environment. Three types of capillaries, differing in their morphology, were found in the composition of the vascular bodies’ villi in Bos taurus taurus.

Experimental shortening of the radius in the treatment of Kienböck's disease

AimThe aim of the research was to determine the smallest amount of loading on the lunate bone obtained by gradually shortening the radius in different ulnar variants as the potential treatment of Kienböck's disease. MethodsThe research was conducted on anatomic preparations of 20 upper extremities using only the distal part of the forearm and hand, placing them in exactly defined positions. A Fuji Prescale film was inserted in the open radiocarpal joint to measure pressure after compressing axially by changing the length of forearm in four stages by the gradual shortening of the radius. ResultsThe greatest reduction of average pressure on the lunatum achieved by shortening the radius depended on ulnar variant. In ulnar variant 0, the greatest reduction of average pressure on lunatum was achieved by shortening the radius for 3 mm (24% reduction). For ulnar variant −1, the greatest reduction of average pressure on lunatum was achieved by shortening the radius for 2 mm (37% reduction). For ulnar variants −2, −3 and +1, the greatest reduction of average pressure on lunatum was achieved by shortening the radius for 3 mm (5%, 75% and 9% respectively). ConclusionsThe pressure distribution in the distal radiocarpal joint is vital for healing, and shortening of the radius relieves the pressure on the lunate bone. The exact type of surgery and the amount of radial osteotomy depends on preoperative ulnar variant.

Anatomical Structure and Terpenoid Content of Zodia (Evodia suaveolens Scheff) Leaves

Zodia (Evodia suaveolens Scheff) is a member of Rutaceae contain terpenoids, triterpenoids, alkaloids, flavonoids, and xanthones which have anti-mosquito activity. This research aimed to observe the anatomical structure, the location, and distribution of terpenoid based on the leaves' age. Anatomical slides preparation of leaves were made using the paraffin embedding method with safranin staining. The distribution of terpenoid was analyzed by the histochemical assay. Leaf anatomical structure shows that the 3rd and 6th leaf bifacial (dorsiventral) consisted of the upper epidermis, mesophyll (palisade and sponge), collateral vascular bundle, parenchyma midrib, abaxial epidermis and oil glands in mesophyll that is underneath both epidermises. The diameter of oil glands with larger sizes was on the 6th leaf, whereas the density is not different in the 3rd and 6th leaves. The histochemical test showed that terpenoid was observed in the leaf vascular bundles, oil glands, and epidermis.

Multiple kidney of the Baikal seal in early postnatal ontogenesis: structure and topograpy

The article presents the results of studies on the anatomical structure of the kidneys of the Baikal seal in early postnatal ontogenesis. A number of morphological features were identified by the anatomical methods (including anatomical preparation, infusion with polyurethane foam through the ureter). The Baikal seal kidneys are multiple, of a bunch shape. They are located in the lumbar zone of the mesogastric at the level of the second, third and fourth lumbar vertebrae. Their weight, width, height, and thickness were measured. Height and width of the kidneys and their structure were determined. By injecting the excretory ducts with polyurethane foam, the structure and size of the renal calyx, length and width of the renal stalk (excretory duct) in early postnatal ontogenesis were determined. The age was determined by annual dentine rings of the canine base (the transverse canine cut) and claw rollers (by the Chapskys method).

Anatomy of facial nerve in maned wolf (Chrysocyon brachyurus - Illiger, 1815)

Abstract: Maned Wolf (Chrysocyon brachyurus) is considered one of the largest and most beautiful canids in South America, inhabits the Cerrado biome, and presents nocturnal habits, especially during the twilight period. It is in danger of extinction, mainly due to anthropic activity in its habitat. What certainly raises the importance of studying and better understanding its biology. The mammalian Facial Nerve is the seventh cranial pair and controls the functions of facial expression muscles and others. Once the anatomical descriptions of this canid are scarce, this paper aimed to describe the anatomy of Facial n. in Maned Wolf and correlate it with that of domestic canids, a phylogenetically close species whose anatomy is well known. For this research, four adult specimens were used, whose carcasses were collected along highways in southeastern Goiás State - Brazil (SISBIO 37072-2), or donated by the Wildlife Screening Center (CETAS) in Catalão - Goiás State. The specimens were fixed in a 10% aqueous formalin solution and stored in the same solution. Dissection was performed by macroscopic anatomical methods. The research was carried out with a favorable opinion of the Animal Use Ethics Committee (CEUA) of the Federal University of Uberlândia (UFU) (under n° 067/12). Anatomical preparations of post-mortem Maned Wolf did not reveal the presence of Caudal Auricular, Internal Auricular, nor Stylohyoid n.. Small ramifications of Facial n. can be considered as collateral branches. The Facial n. emerged as a single trunk, Facial n. Trunk, which branches into Auriculopalpebral, Dorsal Buccal, and Ventral Buccal n..

Морфологические особенности общего покрова у длинноволосых пород кошачьих

The article presents a comparative characteristic of the skin in long-haired cat breeds based on the use of morphological research methods, including anatomical preparation, light microscopy of histological sections, micromorphometry and statistical analysis of the obtained digital data. The general patterns and breed characteristics of the skin in long-haired cats have been established, taking into account its anatomical and topographic features. An inverse relationship was found between the thickness of the epidermis and the thickness of the hair cover in the studied animals, regardless of breed. The specific glomerular design of collagen structures of the dermis was established in all long-haired cat breeds. It has been shown that the depth of the hair follicles and the density of the hair are determined by the topical features of the skin.

THE USING OF NEW PRESERVING SOLUTION FOR THE STORAGE AND THE DEMONSTRATION OF ANATOMICAL PREPARATIONS

For storage and open demonstration of moist anatomical educational preparations and museum exhibits proposed to use a new preservative, which is an aqueous solution of 0.014-0.019% 3-iodine-2-propinilbutilcarbonate. The solution has high antiseptic activity and does not have a local irritant effect on the skin, mucous membranes of the upper respiratory tract and oral cavity, as well as on the conjunctiva of the eyes. The proposed preservative is non-toxic and environmentally safety. Long-term storage and use of anatomical preparations in the proposed preservative does not cause any damaging changes, violations of their shape, structure and color. The method is patented by the Russian Patent Agency.

Cryodehydration Technique Applied to Anatomical Segments

Abstract Introduction The present study describes a variation of the cryodehydration technique, applied to prepare biological tissues by repetitions of section freezing and thawing (SFT). First developed for muscles and then viscera, the aim of this process as presented here is to obtain anatomical segments to be used in anatomy classes, general exhibitions and museums. Materials and Methods The animal, previously fixed in 10% formalin, must be frozen and then sectioned in longitudinal, transverse and horizontal slices of the body-axis. It has two steps: the “burning phase,” in which ice crystal formation and water dilatation cause micro ruptures in the tissue; and the “dehydration phase,” developed to dry out the slices segments slowly and, at the same time, to impregnate tissues with wood glue. Results The obtained slices present several advantages, such as being dry segments, of light weight, and being rapidly used, easily stored and promptly studied. Also, it is possible to study the different organs of one segment and, by apposition, remount an entire animal, enabling a dynamic study. Conclusion This very simple and inexpensive technique produces anatomical preparations with high durability and resistance, which are very helpful in practical and theoretical classes.

Morphological studies of stented coronary artery bifurcations on anatomical preparations of the heart

Introduction. One of the most difficult and completely unsolved problems of modern interventional cardiology is bifurcation stenting of the coronary arteries. This problem requires a comprehensive study, including using the possibility of morphological analysis.Material and methods. Stenting of the bifurcation lesions of the coronary arteries on 46 cadaveric hearts was performed using the bifurcation and conventional stents with subsequent plating and study of the preparations obtained.Results. From the point of view of the safety of the lateral branch, T-stenting is optimal, especially when implanting a BIOSS stent. When using the Tryton stent with the culotte stenting technique, there was never a stenosis of the side branch, due to the stent in the lumen, but there was always an excessive metal saturation in the main branch before the bifurcation. When using conventional stent with a provisional T-stenting technique residual, stenosis of the mouth of the lateral branch was also observed. In addition, in all cases there was a deformation of the stent beams near the mouth of the lateral branch.Conclusions. Morphological studies of stented coronary arteries on anatomical preparations of the heart, plastized with epoxy resin, make it possible to obtain new results that cannot be obtained with traditional morphological and clinical research methods (histological, corrosive, radiological, etc.).

THE ANATOMY OF STRUCTURES OF THE KNEE JOINT AT DEFORMING ARTHROSIS ACCORDING TO THE DATA OF VITAL AND POST-VITAL STUDIES

In the anatomical part of the study on 50 non-embalmed and 50 polymer-embalmed anatomical preparations of the knee joint of an adult were studied the degenerative-dystrophic changes in the bone and auxiliary elements of the knee joint with deforming arthrosis of the I and III stage. The objects of the clinical part of the study were patients with degenerative-dystrophic changes in the knee joint of varying severity who underwent x-ray examination (150 people), magnetic resonance imaging (60 people), diagnostic arthroscopy (35 people). For determine the stage of gonarthrosis in patients, the classification of N.S. Kosinskaya (1961) was used. Identified in the study of anatomical preparations of the knee joint with I stage of gonarthrosis degenerative-dystrophic changes were present in all patients with a similar severity of arthrosis of the knee joint and were confirmed by the results of their additional examination. It study is shown that for the diagnosis of degenerative meniscus ruptures and degenerative changes in the cruciate ligaments of the knee joint, the most informative method is magnetic resonance imaging, and diagnostic arthroscopy does not provide an exhaustive diagnosis of these manifestations. The performed comparison of vital and post-vital morphological manifestations of I stage gonarthrosis, as well as an analysis of the effectiveness of the applied diagnostic techniques convincingly indicates the need for this category of patients to perform x-ray studies and magnetic resonance imaging of the knee joint. When performing magnetic resonance imaging of the knee joint with III stage gonarthrosis trabecular edema of varying severity was found, localized mainly in the subchondral bone tissue of the internal condyles of the tibia and femur, and signs of avascular necrosis of the condyle of the condyle were revealed. The analysis of the intravital diagnostic methods used in patients with III stage gonarthrosis and comparing their results with the data of anatomical studies of knee joint preparations with the same stage of the degenerative-dystrophic process, indicated sufficient visualization capabilities of each from presented methods for additional examination.

Відеоасистовані торакоскопічні лобектомії при туберкульозному, неспецифічному й онкологічному ураженні легенів

Мета. Поділитися власним досвідом виконання відеоасистованих торакоскопічних (ВАТС) лобектомій у пацієнтів з туберкульозним, неспецифічним і онкологічним ураженням легенів за період з 2014 по 2019 р.
 Матеріали і методи. За останні 5 років нами виконано 72 ВАТС резекції частки легені, у тому числі 30 (41,7%) типових ВАТС нижніх лобектомій з роздільною хірургічною обробкою елементів кореня частки. У 1 (1,4%) пацієнта вдалися до конверсії в торакотомію у зв’язку з геморагією. Серед пацієнтів переважали жінки – 46 (63,9%). Вік пацієнтів коливався від 17 до 85 років, у середньому становив (39,2 ± 6,2) року.
 Оперативні втручання було виконано з приводу туберкульозу легенів у 57 (79,2%) пацієнтів, з приводу раку - у 7 (9,7%), вродженої гіпоплазії легені - у 2 (2,8%), артеріовенозної мальформації - у 1 (1,4%), гамартохондроми великих розмірів – у 2 (2,8%), бронхоектатичної хвороби – у 2 (2,8%), внутрішньочасткової секвестрації – у 1 (1,4%) пацієнта.
 Результати. Інтраопераційні ускладнення діагностовані у 3 (4,2%) пацієнтів. Загалом післяопераційні ускладнення виникли у 12 (16,7%) пацієнтів. Загальна ефективність виконання ВАТС лобектомії становила 98,6%, середня тривалість резекційних оперативних втручань – (185,1 ± 72,2) хв, середній об’єм інтраопераційної крововтрати – (154,1 ± 73,8) мл, середня тривалість лікування в післяопераційному періоді – (29,2 ± 14,1) дня, частота конверсії в широку торакотомію - 1,4%.
 Висновки. ВАТС лобектомія є ефективним методом хірургічного лікування хворих, що потребують виконання торакальних операцій, у тому числі фтизіатричного та онкологічного профілів. Даний вид операцій перспективний, малотравматичний, забезпечує анатомічне препарування елементів кореня частки легені, його можна розглядати як альтернативу відкритій лобектомії. Широкому використанню даного методу перешкоджає виражений фіброз кореня легені і злуковий процес внаслідок тривалого запального процесу і тривалого медикаментозного лікування та вартість ендоскопічних інструментів.

History and development of plastination techniques.

Plastination was a game-changing invention for macroscopic anatomical preparation. The method yielded dry, odourless, tangible and durable specimens which allowed new exhibition and teaching set-ups and paved the way for sophisticated preparations and spectacular positioning of specimens. Despite the impact of the new method, there have been similar techniques in place before. Exsiccation techniques, polymer embeddings and specimen impregnation with hardening substances were earlier methods which already included the main concepts that were later combined and refined in plastination. S10 silicone plastination, the technique most commonly known and applied, was followed by plastination methods suitable for research and sectional anatomy teaching. Numerous variations of sheet plastination techniques allow research applications and new ways of presenting topographic relations and mesoscopic insights. Besides the development of plastination techniques in sensu stricto, related techniques had a renaissance with new applications and developments, including corrosion casting and diaphonization methods. This brief review shall provide a historical context of plastination including some anecdotal spotlights on the ideas and innovations that lead to nowadays plastination techniques.

The first record of grass-like fern, Schizaea dichotoma (L.) J. Sm. (Schizaeaceae) from Lingga Island, Indonesia: Its morphological, anatomical and palynological study

Abstract. Sofiyanti N, Iriani D, Fitmawati. 2019. The first record of grass-like fern, Schizaea dichotoma (L.) J. Sm. (Schizaeaceae) from Lingga Island, Indonesia: Its morphological, anatomical and palynological study. Biodiversitas 20: 2651-2660. Schizaea dichotoma (L.) J.Sm. is one of fern species from Family Schizaeaceae. This species is grass-like, dimorphic with fan-shaped fronds, and well-known with local name Rumput Merak. The first record of this species is reported from Lingga Island, Riau Islands Province, Indonesia. The aim of this study was to examine the morphology, anatomy and spore characteristics of this fern. This study had been carried from June to December 2017. Samples were collected using exploration method, documented, and prepared for herbarium specimen. In this study, we examined the morphological characters, anatomical characters of rhizome, stipes, and pinnae of both fertile and sterile fronds, as well as spore feature. The anatomical preparations were carried out using paraffin method, while spore specimens using acetolysis method. The anatomy of rhizome, stipes, and pinnae, as well as spore characters, were then observed and documented using digital microscope Celeron. S. dichotoma is dimorphic fern with grass-like habit, fan-shaped laminae that repeatedly forked end in sorospore in fertile frond. The rhizome has vascular bundle surrounded by star-shaped sclerenchymatous layer. The cross-section of stipes of both fertile and sterile fronds are "M" shaped with a vascular bundle located in the dorsal side, while the leaves have a vascular bundle in the middle of midrib. The type of vascular bundles and stele of those organs are similar, i.a. collateral closed and protostele. We observed The Casparian strip in the endodermis layer of rhizome, stipes, and leaves, as well as at cortex and among the xylem. The spore of S. dichotoma is monolete and large spore class (ca. 59.5 ± 2.4 x 35.86 ± 2.01 µm).