To evaluate objective and subjective outcomes of patients who underwent sacrocolpopexy using autologous rectus fascia to provide more data regarding non-mesh alternatives in pelvic organ prolapse surgery. Ambispective cohort study with retrospective and prospective data. A single academic medical centre. Women who underwent abdominal sacrocolpopexy using autologous rectus fascia between January 2010 and December 2019. Patients were recruited for a follow-up visit, including completing the Pelvic Floor Distress Inventory (PFDI-20) and Pelvic Organ Prolapse Quantification (POP-Q) examination. Demographic and clinical characteristics were collected. Composite failure, anatomic failure, symptomatic failure and retreatment. During the study period, 132 women underwent sacrocolpopexy using autologous rectus fascia. The median follow-up time was 2.2years. Survival analysis showed that composite failure was 0.8% (95%CI 0.1%-5.9%) at 12months, 3.5% (95%CI 1.1%-10.7%) at 2years, 13.2% (95%CI 7.0%-24.3%) at 3years and 28.3% (95%CI 17.0%-44.8%) at 5years. The anatomic failure rate was 0% at 12months, 1.4% (95%CI 0.2%-9.2%) at 2years, 3.1% (95%CI 0.8%-12.0%) at 3years and 6.8% (95%CI 2.0%-22.0%) at 5years. The symptomatic failure rate was 0% at 12months, 1.3% (95%CI 0.2%-9.0%) at 2years, 2.9% (95%CI 0.7%-11.3%) at 3years and 13.1% (95%CI 5.3%-30.3%) at 5years. The retreatment rate was 0.8% (95%CI 0.1%-5.9%) at 12months and 2years, 9.4% (95%CI 4.2%-20.3%) at 3years and 13.0% (95%CI 6.0%-27.2%) at 5years. Autologous rectus fascia sacrocolpopexy may be considered a safe and effective alternative for patients who wish to avoid synthetic mesh. Sacrocolpopexy using autologous rectus fascia is a safe and effective alternative to synthetic mesh.