Anatomical Examination Research Articles

Experimental observation on a new chimney-shaped mechanical valve completely implanted above the mitral annulus in animals.

Current commercially available prosthetic valves suffer from limited size, high requirements for implantation technique, subvalvular structural destruction, and valve dysfunction due to proliferation of fibrous endothelial tissue. This study aims to perform the preclinical large animal experiments for surgically implanting a chimney-shaped artificial mechanical heart valve with zero left ventricular occupancy, which fully accommodates the movement of the valve leaflets in the valve frame and realizes completely supra-annular surgical implantation. A total of 7 sheep underwent the replacement of artificial valve, and 5 sheep survived normally until anatomical examination. The mechanical properties of these artificial mitral valves remain functionally normal. There was no obvious thromboembolism around the artificial valve and in the important organs. The tissue layer of suture ring was completely organized and endothelialized, and the thickness of tissue layer was about 0.6-1.0mm. The follow-up of echocardiography showed that the left ventricular ejection fraction was normal (60-70%) before and 6months after operation. The results of transvalvular pressure gradient and blood flow velocity of artificial valve were normal. Left ventricular retrograde angiography showed that the artificial valve was completely located in the left atrium with good position and normal opening and closing. There was no obvious perivalvular leakage and other abnormalities. At 3 and 6months, there were no obvious abnormalities in blood routine test, liver and kidney function, and other indexes. The new chimney-shaped artificial mechanical valve implanted completely above the mitral annulus had good wear resistance, histocompatibility, and antithrombotic and hemodynamic performance.

Spot(ters) the difference: Bringing traditional anatomical examinations online.

The COVID-19 pandemic caused a shift in anatomy education forcing institutions to find innovative ways to teach and assess online. This study details the development of an online spotter across multiple modules that allowed students to sit the examination at home whilst still maintaining the integrity of the assessment. The online spotter consisted of individual, Zoom calls between students and examiners whereby slides with images and questions were screen shared. To examine the viability of this spotter in non-lockdown scenarios several parameters were considered. Mean marks were compared to traditional versions and Pearson's r correlation coefficients were calculated between online and traditional spotters and between online spotters and overall performance in anatomy modules. A survey was carried out to determine the students' view of the assessment. Pearson's r was between 0.33 and 0.49 when comparing online spotters to the traditional format, and between 0.65 and 0.75 (p < 0.01) when compared to a calculated anatomy score. The survey indicated overall student satisfaction as 82.5% reported that it was a fair way to test their knowledge and 55% reported the same or lower levels of anxiety when compared to traditional spotters. However, there was nothing to indicate that the students preferred this format over laboratory-based spotters. These results indicate that this new exam format would be useful for small cohorts who are undertaking online or hybrid courses, or in circumstances when running a full spotter is too costly, and represents a fair and robust way to assess practical anatomical knowledge online.

Methyl jasmonate, salicylic acid, and oxalic acid affects growth, inducible defenses, and pine weevil resistance in Norway spruce.

The large pine weevil (Hylobius abietis) is a major regeneration pest in commercial forestry. Pesticide application has historically been the preferred control method, but pesticides are now being phased out in several countries for environmental reasons. There is, thus, a need for alternative plant protection strategies. We applied methyl jasmonate (MeJA), salicylic acid (SA) or oxalic acid (OxA) on the stem of 2-year-old Norway spruce (Picea abies) plants to determine effects on inducible defenses and plant growth. Anatomical examination of stem cross-sections 9 weeks after application of 100 mM MeJA revealed massive formation of traumatic resin ducts and greatly reduced sapwood growth. Application of high concentrations of SA or OxA (500 and 200 mM, respectively) induced much weaker physiological responses than 100 mM MeJA. All three treatments reduced plant height growth significantly, but the reduction was larger for MeJA (~55%) than for SA and OxA (34-35%). Lower MeJA concentrations (5-50 mM) induced comparable traumatic resin duct formation as the high MeJA concentration but caused moderate (and non-significant) reductions in plant growth. Two-year-old spruce plants treated with 100 mM MeJA showed reduced mortality after exposure to pine weevils in the field, and this enhanced resistance-effect was statistically significant for three years after treatment.

Island Pedicle Flap Pada Dinding Hidung Dengan Karsinoma Sel Basal

A flap is a tissue unit that is transferred from one area (donor site) to another area (recipient site) while still maintaining its blood circulatory system. The island pedicle flap, also known as the V-Y flap, is a modified advancement flap. This flap is a very useful reconstruction option for small to intermediate-sized defects. A 69-year-old Acehnese woman with a history of working as a farmer came with complaints of a non-painful nodule on the wall of her left nose for the last 6 months. Based on the history and physical examination, the patient was provisionally diagnosed with nodular type Basal Cell Carcinoma. An excisional surgical was performed, then a pedicle island flap was performed to close the defect. Anatomical pathology examination results concluded basal cell carcinoma. The island pedicle flap technique is one of the surgical techniques that can be an option to close tumor excision defects. This technique offers a one-stage reconstruction with excellent cosmetic results for defects in the nasal wall. The color and texture of the skin after surgery are almost perfect because the flap originates from the skin immediately adjacent to the defect.

Analysis of mechanical and anatomical properties of two bamboo species – Thyrostachys siamensis Gamble and Dendrocalamus strictus (Roxb.) Nees (Poaceae)

Bamboos are treated to boost their shelf life from the degrading activity of fungal microbes and other insects and to increase their longevity in use and durability by using various chemicals. The present study is vis-a-vis the standardization of two bamboo species Dendrocalamus strictus and Thyrostachys siamensis for a comparative analysis of the mechanical and anatomical properties of samples treated with CCB (Copper Chrome Boron), and BBA (Borax Boric Acid) as well as untreated samples.Samples were tested for both bending and compression; and the parameters such as MOE (Modulus of Elasticity), MOI (Moment of Inertia), Maximum compressive strength, and ultimate strength were analyzed. The anatomical variations arising in the top and bottom sections were also investigated and it has found that the mechanical properties of the species treated with CCB solutions yield a better result in maximum compressive strength and high value of MOI as compared to BBA whereas the samples treated with BBA provides superior performance with respect to MOE. Anatomical examination revealed that the cellular structure and arrangement of vascular bundles are responsible for the increase in time taken for the rate of penetration of chemicals in D.strictus i.e one-half hour as compared to T.siamensis which got treated in 20 minutes. In D.strictus the density of vascular bundles is high so it takes time to get it treated completely as compared to T. siamensis.

Anatomical oropharyngeal cavity specialisations in the cutlassfish (Trichiurus lepturus, Linnaeus, 1758).

Trichiurus lepturus is a carnivorous fish, and most of the previous anatomical research has focused on computed tomography imaging and histology of their teeth and fangs, while the remaining structures of pharyngeal cavity remain unexplored. The present research is the first to use anatomical examinations alongside scanning electron microscopy to investigate the T. lepturus oral cavity. The oropharyngeal roof included teeth, upper lip, rostral and caudal velum and the palate. The middle of the palate showed a median groove flanked by two folds, followed by a median band flanked by micro-folds, thereafter the palate became crescent shaped. The lateral regions of the palate exhibited longitudinal folds that extended rostrally towards the fangs. The oropharyngeal floor had two cavities which acted as a scabbard for the premaxillary fangs and upper velum, while the caudal sublingual cavity contained two oyster-shaped structures on the outer surface plus sublingual ridges and sublingual clefts. The tongue apex exhibited a spoon-like shape, its body demonstrated a median elevation and the root with two lateral branches contained only dome-shaped papillae. Taste buds were located on the upper velum, lower lip and the caudal part of the interbranchial septum. Images and descriptions of T. lepturus tooth structure are also provided. The present research, using anatomical dissection and morphological observation using scanning electron microscopy, has identified the structures of the dentition system, a variety in shapes of the folds and microridges, and identified the taste buds and mucous pores in the T. lepturus oropharyngeal cavity.

Morphologic and Morphometric Study of Lungs in Adult Indigenous Peafowl ‎‎(Pavo cristatus)

&#x0D; &#x0D; &#x0D; &#x0D; Peafowl are a species of bird for which there is limited scientific research, despite the ‎importance of the respiratory system on bird health. This study aimed to provide a detailed ‎anatomical examination and comparative description of the lungs in adult male and female ‎indigenous peafowl (Pavo cristatus) to further our understanding of the respiratory system in ‎this species. Sixteen healthy adult peafowl (8 peacocks and 8 peahens) were obtained from ‎commercial markets in Dyalla, and Baghdad, Iraq. The birds had a mean live weight of 3.5±0.59 kg for females ‎and 3.0±0.39 kg for males. After being anesthetized, the birds were sacrificed by cutting off major ‎blood vessels in the neck. Subsequently, the lungs were carefully dissected and examined to ‎identify their shape, color, position, dimensions, and overall structure. Detailed photographs of ‎the lungs were taken, and various measurements were recorded for each lung in male and ‎female peafowl. The lungs of the peafowl were found to have an elongated, triangular shape, ‎extending from the first to the sixth vertebral ribs. Each lung exhibited two borders (medial and ‎lateral) and three surfaces (vertebral, costal or dorsal, and septal). Notably, the lateral border ‎and dorsal surface of each lung had four deep grooves resulting from the embedding of the ‎second to fifth ribs, which led to the division of the lung into five lobes in both peacocks and ‎peahens. To the best of our knowledge, this study represents the first scientific research on the ‎anatomical features of the lungs in indigenous peafowl. The findings from this study contribute ‎to a better understanding of the lung structure in this bird species, which can be valuable for ‎future studies on avian respiratory physiology and health.‎‎‎&#x0D; &#x0D; &#x0D; &#x0D;

An essential overview of orofacial clefting.

An appreciation of the embryologic development of the face is essential to understanding the anatomic variation observed in this phenotypically broad condition. Embryologically, the nose, lip and palate are separated into the primary and secondary palate, which are divided anatomically by the incisive foramen. The epidemiology of orofacial clefting is reviewed together with the contemporaneous cleft classification systems that enable comparisons to be made between international centres for audit and research purposes. A detailed examination of the clinical anatomy of the lip and palate informs the surgical priorities for the primary reconstruction of both form and function. The pathophysiology of the submucous cleft palate is also explored. The seismic impact of the 1998 Clinical Standards Advisory Group report on the organisation of UK cleft care provision is outlined. The importance of the Cleft Registry and Audit Network database in auditing UK cleft outcomes is highlighted. The potential for the Cleft Collective study to establish the causes of clefting, the optimal treatment protocols, and the impact of cleft on patients is tremendously exciting for all health care professionals involved in the management of this challenging congenital deformity.

A neonatal rat model of pulmonary vein stenosis

ObjectivesPulmonary vein stenosis (PVS), one of the most challenging clinical problems in congenital heart disease, leads to secondary pulmonary arterial hypertension (PAH) and right ventricular (RV) hypertrophy. Due to the lack of a rodent model, the mechanisms underlying PVS and its associated secondary effects are largely unknown, and treatments are minimally successful. This study developed a neonatal rat PVS model with the aim of increasing our understanding of the mechanisms and developing possible treatments for PVS.MethodsPVS was created at postnatal day 1 (P1) by banding pulmonary veins that receive blood from the right anterior and mid lobes. The condition was confirmed using echocardiography, computed tomography (CT), gross anatomic examination, hematoxylin and eosin (H&E) staining, fibrosis staining, and immunofluorescence. Lung and RV remodeling under the condition of PVS were evaluated using H&E staining, fibrosis staining, and immunofluorescence.ResultsAt P21, echocardiography revealed a change in wave form and a decrease in pulmonary artery acceleration time—indicators of PAH—at the transpulmonary valve site in the PVS group. CT at P21 showed a decrease in pulmonary vein diameter in the PVS group. At P30 in the PVS group, gross anatomic examination showed pulmonary congestion, H&E staining showed wall thickening and lumen narrowing in the upstream pulmonary veins, and immunofluorescence showed an increase in the smooth muscle layers in the upstream pulmonary veins. In addition, at P30 in the PVS group, lung remodeling was evidenced by hyperemia, thickening of pulmonary small vessel walls and smooth muscle layers, and reduction of the number of alveoli. RV remodeling was evidenced by an increase in RV free wall thickness.ConclusionsA neonatal rat model of PVS was successfully established, showing secondary lung and RV remodeling. This model may serve as a useful platform for understanding the mechanisms and treatments for PVS.

The continuous structure of the joint capsule and meniscus in the pig knee.

The knee joint capsules composed of a fibrous layer and a synovial layer. The knee meniscus consists of the superficial network, lamellar layer, tie fibre and circumferential bundles. However, the continuous structure of the knee joint capsule and meniscus has not been reported. Fetal and adult pigs were used to investigate the structural relationship between the stifle joint capsule and meniscus based on the gross anatomy and histological findings. In the gross anatomical examination, the joint capsule appeared to have separated attachments to the meniscus, except for the lower aspect of the popliteal hiatus. Histologically, the lower half of the popliteal hiatus was found to have separated attachments, with vessels running between the attachments of the joint capsules. The synovial layer of the joint capsule continued to the superficial network, and the fibrous layer of the joint capsule continued to the lamellar layer and tie fibres. There were two routes of arterial entry into the meniscus: intracapsular and intercapsular. It appeared that the presence of separated attachments of the joint capsule was necessary to allow the intercapsular route. This study clarified for the first time the routes of feeding vessels entering the meniscus and proposed to call this entry point the meniscus hilum. We consider that this detailed anatomical information is important for understanding the continuation between the joint capsule and the meniscus.

Pictorial Essay of Linear Endoscopic Ultrasound Examination of Pancreas Anatomy

AbstractEndoscopic ultrasound (EUS) is a widely used imaging modality for both diagnostic and therapeutic purposes. Understanding the anatomy is crucial during a curved linear EUS examination. Compared with other advanced endoscopic techniques, the learning curve for EUS is longer, and the training facilities for EUS are also not widely available. The interest and enthusiasm for EUS among endoscopists is limited by the long learning curve and the scarcity of training programs. Imaging of the pancreas is the most common indication of EUS examination, and many endoscopist often face difficulty in understanding the anatomy and orientation of the pancreas on linear EUS examination. In this article, we will discuss the problems encountered during linear EUS examination and how to overcome each problem with a station-wise pancreas examination.

Use of underwater contactless ultrasonography to elucidate the internal anatomy and reproductive activity of manta and devil rays (Fam. Mobulidae).

The ability to visualise the internal anatomical structures of fish provides important information on their reproductive status and body condition and has made important contributions to many areas of fish biology. Obtaining information about the internal anatomy of fish has traditionally required euthanasia and dissection. However, although ultrasonography is now increasingly used to study internal fish anatomy without the need for euthanasia, traditional techniques still require restraint and contact with the animal, both of which are known to cause stress. This has prompted the development of waterproof, contactless, and portable equipment to allow ultrasonographic examinations to be carried out in free-swimming individuals which also facilitates the application of this tool in wild populations of endangered species. This study reports the validation of this equipment using anatomical examinations of nine manta and devil ray (Mobulidae) specimens landed at fish markets in Sri Lanka. Species studied were Mobula kuhlii (n = 3), M. thurstoni (n = 1), M. mobular (n = 1), M. tarapacana (n = 1) and M. birostris (n = 3). The use of this equipment was further validated with ultrasonographic examinations in 55 free-swimming reef manta rays Mobula alfredi, which enabled maturity status to be quantified in 32 females. Structures successfully identified in free-swimming individuals were the liver, spleen, gallbladder, gastrointestinal tract, skeletal structures, developing follicles and uterus. The study demonstrated that ultrasonography provided a reliable method of determining both sexual maturity and gestational status in free-swimming M. alfredi. The methodology induced no detectable signs of disturbance to the animals involved and therefore offers a viable and practical alternative to invasive techniques currently used to study anatomical changes in both captive and wild marine organisms. This article is protected by copyright. All rights reserved.

The Anterior Cingulate Cortex Promotes Long-Term Auditory Cortical Responses through an Indirect Pathway via the Rhinal Cortex in Mice.

Sensory cortical areas are robustly modulated by higher-order cortices. Our previous study shows that the anterior cingulate cortex (ACC) can immediately and transiently enhance responses in the mouse auditory cortex (ACx). Here, we further examined whether strong activation of ACC neurons can induce long-term effects in mice of both sexes. To our surprise, only stimulation of cell bodies in the ACC, but not ACC-to-ACx terminal activation, induced long-term enhancement of auditory responses in the ACx. Anatomical examination showed that the ACC indirectly projects to the ACx via the rhinal cortex (RCx). High-frequency stimulation of ACC-projecting terminals to the RCx or RCx-projecting terminals to the ACx induced a similar effect as the cell body activation of ACC neurons, whereas silencing the RCx blocked this long-term enhancement. High-frequency stimulation of ACC projections to the RCx also induced long-term augmentation of sound-evoked flight behavior in male mice. These results show that the ACC promotes the long-term enhancement of auditory responses in the ACx through an indirect pathway via the RCx.SIGNIFICANCE STATEMENT In this study, we demonstrate that the anterior part of the anterior cingulate cortex (ACC) evokes long-term enhancement of auditory responses in the auditory cortex (ACx) when it is strongly activated. Importantly, instead of a direct projection, we show that the ACC implements this effect via an indirect pathway through the lateral rhinal cortex using a series of physiological, optogenetic, anatomic, and behavioral experiments. Along with a short-term effect, this long-term enhancement induced by an indirect ACC-to-ACx projection could increase the odds of survival when animals are faced with threats after a significant event.

Histopathological Lesions in Gastric and Duodenum Organs in Dogs (Canis familaris) Suspected of Escherichia coli Infection

It is widely known that Escherichia coli (E. coli) can infect the gastrointestinal tract of various animals and damage some tissue organs. Through histopathological examination of the gastric and duodenal organs can provide valuable information relating to how the E. coli infection impacts the animal. On February 6th, 2023, euthanasia and necropsy of a dog belonging to Mr. Kamo Agil were carried out, which is located at Jl. Meyjend Sungkono, Gresik. Anamnesa is known to be a male dog, age ±1.5 months, body weight 0.6 kg. One kennel is filled with 9 dogs, dirty cage, in the house is not unused, damp and close to the warehouse area and furniture office. The dog has never been vaccinated and has not been dewormed. Temporary diagnosis of dogs with colibacillosis. Examination of pathology is carried out macroscopically and microscopically. Microbiological examination with isolation of escherichia coli bacteria and eosin methylene blue agar media. Followed by Gram staining and biochemical tests. The results of anatomical pathology examination showed that the dog had gastroenteritis. The results of microbiological examination showed that isolated and identified escherichia coli from duodenal organs. Based on the results of examinations showed that the dog had gastroenteritis caused by caused by escherichia coli infection.

Lingual abscess in a South American tapir (Tapirus terrestris): A diagnostic challenge

AbstractAn 18‐year‐old, male South American tapir (Tapirus terrestris) presented with acute oropharyngeal damage beginning with moderate macroglossia, ptyalism and dysphagia, and rapidly progressing to severe macroglossia with hypoglossal nerve sensory and motor damage. Investigations did not allow the authors to find the origin of this damage and, in view of the deterioration of the general state and wellbeing of the animal despite the care provided, euthanasia was carried out. Anatomical and histopathological examination revealed a lingual and sublingual abscess of undetermined origin associated with lesions of bacterial interstitial pneumonia, probably secondary to dysphagia. This case illustrates the diagnostic failure of investigations performed, including submandibular ultrasound by a ventral approach and fine‐needle aspiration, which are supposed to be efficient in the diagnostic of lingual abscesses.

Diagnosis of Marek’s Disease in Isa Brown Layer Chicken based on Anatomical Pathology and Histopathological Examination

Marek’s disease and reticuloendotheliosis are neoplastic diseases in poultry infected by Marek’s disease virus (MDV) and reticuloendotheliosis virus (REV), respectively. This infection may lead to tumor growth in the chicken organ which is difficult to distinguish based on macroscopic lesions, therefore it is important to further diagnose by histopathological examination. Thus, this study aimed to diagnose Marek’s disease in layer chicken based on anatomical pathology and histopathology examination. The samples used were ten cadavers of culled Isa Brown chicken that died in the poultry market. The samples were immediately transported to Pathology Laboratory for necropsy and macroscopically examination. The tumors observed on the pancreas and mesenteric organs were collected and then proceeded to histopathological examination followed by staining using hematoxylin-eosin (HE) and Masson’s trichrome (MT). The anatomical pathology examination showed numerous white-colorized tumors which vary in size found in the mesenteries and pancreas. Histopathological examination demonstrated that tumors consisted of inflammatory cells and reticular cells. The inflammatory cells were pleomorphic and basophilic lymphocytes. In conclusion, in this study, the diagnosis of the visceral type of Marek’s disease in ISA brown layer chicken by means of the anatomical pathology examination was characterized by tumor nodules in the pancreas and mesenteric organs. While microscopically the tumor tissue in Marek’s disease consists of connective tissue and lymphocytes with large cell sizes, varying shapes, and basophilic colors.

Identification of KRAS mutation and HER2 expression in Indonesian colorectal cancer population: a cross-sectional study.

This research is a cross-sectional study. The research subjects in this study were colorectal cancer patients in the digestive surgery division. There were 58 study subjects. Examination of KRAS mutations was carried out by PCR on fresh tumor tissue obtained from surgery or colonoscopy. Meanwhile, the HER2 examination used the immunohistochemistry method of paraffin blocks for anatomical pathology examination. Examination of KRAS mutations showed 28/58 (43.8%) patients with colorectal cancer, while HER2 overexpression was found in 6/58 (10.3%) patients with colorectal cancer. Univariate analysis of KRAS mutations and HER2 expression showed that four subjects with KRAS mutations had excess HER2 expression (P=0.341). There is no association between KRAS mutations and HER2 overexpression in colorectal cancer patients.

Anatomical and epidemiological examination of the structure and localization of osteophytes of cervical vertebrae in geriatric patients by using computed tomography method

Background: To evaluate the osteophytes seen in the cervical vertebrae of geriatric individuals in various parameters. Materials and Methods: 764 (M: 413, F: 351) individuals who were admitted to the State Hospital Radiology Outpatient Clinic with various complaints between the years 2018 and 2023 and who met the criteria were examined in our study. A total of 1209 osteophytes were found in the individuals. The osteophytes were evaluated in terms of gender, age, grade, vertebral level, vertebral spacing, anatomical aspect, anatomical surface and length parameters. Results: Osteophyte formation increased with age in individuals (p&amp;lt;0.05). Osteophyte formation was observed more frequently in men when compared to women (p&amp;gt;0.05). Claw type osteophytes were observed commonly in all individuals (p&amp;lt;0.05). While fused type osteophytes were observed commonly in men, traction-type osteophytes were observed more frequently in women (p&amp;lt;0.05). Osteophyte formation of grade 2 severity was observed in all individuals (p&amp;gt;0.05). While fused osteophytes were commonly observed at grade 4 severity, claw and traction type osteophytes were observed at grade 2 severity (p&amp;lt;0.05). Osteophytes were commonly observed at the C5 vertebra level in all individuals (p&amp;gt;0.05). Fused and traction type osteophytes were commonly seen at the C6 level, while claw type osteophytes are commonly seen at the C5 level (p&amp;lt;0.05). In all individuals, osteophytes were most common in the C5-6 space, followed by the C4-5, C6-7 (p&amp;gt;0.05). Osteophytes were commonly seen anteriorly in both sexes and all osteophyte types (p&amp;lt;0.05). All osteophyte types were commonly observed in the inferior surface of the vertebra (p&amp;lt;0.05). Males had relatively longer osteophytes (mm) than females (p&amp;lt;0.05). Claw type osteophyte was observed in longer dimensions than traction type osteophyte (p&amp;lt;0.05) Conclusions: Osteophyte type and grade severity were found to vary between individuals. In the examinations, varying features were observed in the vertebral level, anatomical aspect, surface and length parameters of the osteophytes.

Study of Laboratory Diagnosis of Colibasilosis Infection In Local Hen In Surabaya

Background: On November 18, 2022, euthanasia and hen necropsy were conducted (protocol number A-164) in the veterinary pathology laboratory, Faculty Of Veterinary Medicine, Wijaya Kusuma University Surabaya. The hen belongs to a local farmer located at Sawahan, Surabaya. Based on the anamnesis, it is known that the hen was four months old and weighed 1.4 kg. The population consists of eight birds, and one of them was sick, never treated or vaccinated, in a cage made of bamboo, corn fodder, rice bran, and drinking water from tap water. Clinical symptoms show a decreased appetite for the last day, watery eyes, runny nose, lethargy, and diarrhea with feces green mixed with white colors. The initial diagnosis of the chicken was infected with Salmonella sp. with a differential diagnosis of Escherichia coli. Purpose: To diagnose bacterial disease in local chickens with a pathological examination, parasitological examination, microbiological isolation, and clinical pathology for hematology. Result: The results of the anatomic pathology examination showed that the chickens had hemorrhagic tracheitis, hemorrhagic necrotic pneumonia, hemorrhagic necrotic enteritis, hemorrhagic necrotic hepatitis, and hemorrhage in the proventriculus. Parasite identification was found in Raillietina sp. and Ornithonyssus sylviarum. Isolation of bacteria was found in Escherichia coli from the duodenum organ. The chickens had hypoproteinemia, poikilocytosis teardrop and rouleaux, leukocytosis with heterophilia, and monocytosis. Conclusion: Based on the examination associated with the results of anamnesis and examination of clinical symptoms, the chickens were diagnosed with Escherichia coli infection together with Raillietina sp. and Ornithonyssus sylviarum infestations.

Different Clinicopathological Characteristics in Indonesian Colorectal Patients with NRAS Mutations and HER2 Over-Expression.

This study aims to assess the association of subject characteristics and NRAS mutations with HER2 expression in CRC. This research is a cross-sectional study. The research subjects in this study were colorectal cancer patients in the Digestive Surgery division at Dr. Hasan Sadikin Hospital. There were 58 study subjects. Examination of NRAS mutations was carried out by Polymerase Chain Reaction (PCR) from fresh tumour tissue obtained from surgery or colonoscopy. Meanwhile, HER2 examination used the Immunohistochemistry (IHC) method of paraffin blocks for anatomical pathology examination of the same patients. HER2 overexpression was found in 6/58 (10.3%) patients with CRC, and from 8 subjects with NRAS mutations, only 1 subject (1.7%) showed overexpression of HER2. Univariate analysis of HER2 expression showed no significant associations to age, sex, histologic feature, tumor location, and NRAS mutations. A significant association was found between HER2 expression and stage of the CRC with p=0.001. There is no association between NRAS mutations and HER2 overexpression in colorectal cancer patients.