Anatomical Analysis Research Articles

How pollen and ovule development underlay dioecy in Chloroluma gonocarpa (Sapotaceae).

Previous studies have determined that Chloroluma gonocarpa (Sapotaceae), is a species that has cryptic dioecy. This type of sexual system is characterized by flowers that are morphologically perfect (both sexual whorls are present) but functionally pistillate or staminate (in each type of flower one of the sexual whorls is non-functional). In C. gonocarpa the pistillate flowers present well-developed stigma, functional ovules, and staminodes, while the staminate flowers present a poorly developed stigma, collapsed ovules, and pollen-producing anthers. In angiosperms, the abortion of sexual organs can occur at different stages of development (from pre-meiosis to post-meiosis), that is why we conducted an anatomical analysis of both flower types at various developmental stages. Using light microscopy, we described the processes of sporogenesis and gametogenesis to establish when the staminate flowers lose their pistillate function. To achieve this, we collected, fixed, and processed the flowers following conventional anatomical techniques for observation under a light microscope. Our findings reveal that pollen development occurs only in staminate flowers, while ovule development begins in both types of flowers but ceases in staminate flowers due to post-meiosis abortion. In contrast, normal development continues in pistillate flowers. These results suggest that dioecy in C. gonocarpa may have arisen from a gynodioecious pathway.

Anatomical and Histological Analyses of Rare Pancake Kidney

Anatomical and Histological Analyses of Rare Pancake Kidney

Mating system, morphological and genetic evidence endorse clonality as an essential reproductive mode in Daphnopsis filipedunculata (Thymelaeaceae), a dioecious and endemic species from the Amazon.

Background and Aims: Clonality is characterized by the formation of independent individuals of the same genotype that are capable of reproducing and propagating vegetatively. Although clonality is an important mechanism that facilitates the persistence of a population, its extensive use can lead to negative impacts on sexual reproduction due to trade-offs in the investment of resources. Therefore, studies on the sexual reproduction of species that exhibit clonality can provide information about resilience to environmental changes, information about fecundity, the risk of the absence of pollinators and the ability to persist in unfavourable conditions and to successfully occupy new areas. Here, we investigated the role of clonal propagation and sexual reproduction in Daphnopsis filipedunculata (Thymelaeaceae), a dioecious species distributed only in Serra dos Carajás. Methods: We evaluated the extent of clonality in this species using molecular tools and anatomical analyses of the underground system responsible for developing new ramets. Furthermore, we analysed the sexual system and its contribution to reproductive success through morphometric analyses of floral types and pollination experiments in the field. Key Results: Overall, we found that clonal propagation plays an important role in maintaining the population of D. filipedunculata. Specifically, we demonstrated that this species presents functional male and female plants, indicating that D. filipedunculata is an obligate xenogamous species but has low reproductive success. We also showed that clonal vegetative propagation is the main form of asexual reproduction in this species, with roots responsible for clonal growth. Finally, our results indicated that this species presents an intermediate phalanx-guerrilla clonal architecture. Conclusions: Our study provides the first insights into sexual reproduction and clonal propagation in D. filipedunculata and can inform management practices, conservation and the restoration of endemic species.

Differential symbiotic compatibilities between rhizobium strains and cultivated and wild soybeans revealed by anatomical and transcriptome analyses.

Various species of rhizobium establish compatible symbiotic relationships with soybean (Glycine max) leading to the formation of nitrogen-fixing nodules in roots. The formation of functional nodules is mediated through complex developmental and transcriptional reprogramming that involves the activity of thousands of plant genes. However, host transcriptome that differentiate between functional or non-functional nodules remain largely unexplored. In this study, we investigated differential compatibilities between rhizobium strains (Bradyrhizobium diazoefficiens USDA110 Bradyrhizobium sp. strain LVM105) and cultivated and wild soybeans. The nodulation assays revealed that both USDA110 and LVM105 strains effectively nodulate G. soja but only USDA110 can form symbiotic relationships with Williams 82. LVM105 formed pseudonodules on Williams 82 that consist of a central nodule-like mass that are devoid of any rhizobia. RNA-seq data revealed that USDA110 and LVM105 induce distinct transcriptome programing in functional mature nodules formed on G. soja roots, where genes involved in nucleosome assembly, DNA replication, regulation of cell cycle, and defense responses play key roles. Transcriptome comparison also suggested that activation of genes associated with cell wall biogenesis and organization and defense responses together with downregulation of genes involved in the biosynthesis of isoprenoids and antioxidant stress are associated with the formation of non-functional nodules on Williams 82 roots. Moreover, our analysis implies that increased activity of genes involved in oxygen binding, amino acid transport, and nitrate transport differentiates between fully-developed nodules in cultivated versus wild soybeans.

17 Prognostic Factors for Local Failure and Overall Survival in Patients with Epidural Disease at the Cauda Equina Following Stereotactic Body Radiotherapy: A Clinical, Anatomic and Dosimetric Analysis

17 Prognostic Factors for Local Failure and Overall Survival in Patients with Epidural Disease at the Cauda Equina Following Stereotactic Body Radiotherapy: A Clinical, Anatomic and Dosimetric Analysis

Anatomical Analysis of the Superior Gluteal Artery in 100 Women for Superior Gluteal Artery Perforator Flap Breast Reconstruction.

The superior gluteal artery (SGA) perforator (SGAP) flap is used more rarely for breast reconstruction with autologous tissue than other flaps because the SGA is often narrow, and the SGAP can be short. However, it provides ample fat, including in lean women. To improve its safety and utility, the preoperative SGA course in women who underwent autologous breast reconstruction was determined with three-dimensional computed tomography angiography. Consecutive deep inferior epigastric perforator, profunda artery perforator, and SGAP flap cases in 2019-2023 were identified. Frequencies of the following favorable preoperative SGA-anatomy variables were determined: branching of the main-SGA trunk on top/posterior of the greater sciatic foramen (designated M1/2), which allows access to a sufficiently wide SGA artery; superolateral perforating location of the superficial-SGA branch (SP3), which means the perforator is sufficiently long for uncomplicated flap placement; and nonbranching of the deep-SGA branch (D1/D2), which means this branch can used a venous and an arterial graft to extend an insufficiently long perforator. A total of 100 cases of deep inferior epigastric perforator (n = 80), profunda artery perforator (n = 13), and SGAP flap-based breast reconstructions (n = 7) were identified. Out of 200 buttocks, 89%, 91.5%, and 62% had the favorable M1/2, SP3, and D1/D2 variables, respectively. An atypical descending branch feeding the lower buttocks (DES1/2) was observed in 34%. The branching position of the main-SGA trunk, perforating location of the SGAP, and the shape of the deep branch were classified in detail in 100 patients. By creating a surgical plan that understands the anatomy taking preoperative three-dimensional computed tomography angiography, the SGAP flap can accommodate many patterns, increasing the possibility of safe execution.

Anatomical analysis of proximal tibia: selecting ideal sites for pediatric intraosseous infusion

Anatomical analysis of proximal tibia: selecting ideal sites for pediatric intraosseous infusion

An anatomical analysis of the occipital nerve complex: an essential tool for the application of occipital nerve blocks

BackgroundOccipital nerve blocks are essential in diagnosing and treating headache disorders such as migraine, cervicogenic headache, occipital neuralgia, and cluster headache. In this study, we aimed to investigate the potential compression points of the greater occipital nerve (GON), third occipital nerve (TON), and lesser occipital nerve (LON) which are targeted to block in occipital nerve blocks and to develop a method to detect these points easily.MethodsTo identify potential compression points of the GON, TON, and LON, we dissected 43, 41, and 26 cadavers, respectively. A rigid, transparent tool divided into 1 × 1 cm sections was placed on the external occipital protuberance to measure the determined points. The cadaveric head was viewed from above, vertically, and the coordinates corresponding to each point were noted separately.ResultsSix, four, and one potential entrapment points were detected for the GON, TON, and LON, respectively. The distances of the point where the GON arose from the lower border of the obliquus capitis inferior muscle and the emerging point of the TON from the C2-C3 vertebrae to the posterior midline were statistically significant in terms of the sides (p = 0.040). Similarly, there was a statistical significance between genders for the distance of the point where the LON arose from the posterior edge of the sternocleidomastoid muscle to the posterior midline (p = 0.002).ConclusionsWe believe that with the method developed, the GON, TON, and LON compression points can be easily localized and blocked in diagnosing and treating patients experiencing headaches such as migraines, cervicogenic headaches, occipital neuralgia, and cluster headache.

A Comparative Morphological and Anatomical Study of Juniperus communis L., J. sibirica Burgsd., and J. pygmaea K. Koch from Bulgaria.

Of the six juniper species found in the Bulgarian flora, three of the species have controversial taxonomic positions. Juniperus pygmaea K. Koch and J. sibirica Burgsd. exhibit similar morphological characteristics to J. communis L. in terms of leaves and female cones (galbuli). This is one of the reasons why, in the recent taxonomic developments, J. pygmaea and J. sibirica were united in a common variety of J. communis, namely, J. communis var. saxatilis. However, such a grouping of species in the Flora of Bulgaria has not been adopted. This study aimed to evaluate the degrees of similarity or difference in the structure of the leaves, galbuli, seeds, and pollen of J. communis, J. sibirica, and J. pygmaea using the methods of comparative anatomy by light microscope (LM) and scanning electron microscopy (SEM) observations and complex morphological measurements. The working hypothesis of this study was that the three species would show a different degree of similarity with each other, which would clarify their taxonomic rank. The morphological parameters revealed differences between the length/width ratio of galbuli and seed length of the three species, while leaf characteristics (length and width) showed a stronger resemblance between J. sibirica and J. pygmaea. Furthermore, a greater distinction between the leaves and galbili of J. communis and J. sibirica was found. The SEM analyses showed variations in the seed shape and spermoderm among the three species. The shape of J. communis seeds was oval and elongated, while J. pygmaea seeds were pear-shaped, and J. sibirica seeds were triangular-rhombic. The length and height of striations were diverse on seed spermoderm in the three species. The epicuticular waxes of leaves, located on the tips of the anticlinal walls of the elongated epidermal cells in J. pygmaea and J. communis, were oval, while they formed raised comb-like crystals in J. sibirica. The morphological, anatomical, and SEM analysis affirmed the accepted taxonomic status of J. communis and J. sibirica as independent species within the Bulgarian flora. Based on most of the analyzed parameters, J. pygmaea exhibits significant similarity with J. sibirica. Additionally, the similar habitats of these two species support the determination of J. pygmaea as a variety or form of J. sibirica rather than J. communis (J. sibirica forma pygmaea).

Molecular evidence for the origin of the little-known hybrid Potamogeton ×vilnensis

A combined study of morphology, stem anatomy, and the genetic markers (ITS1-ITS4 region of nuclear ribosomal DNA and rpl20-rps12 intergenic spacer of chloroplast DNA) was used to verify the identity of hybrid Potamogeton ×vilnensis Galinis previously recognized as P. gramineus × P. praelongus. Authentic herbarium material and newly collected specimens morphologically similar to the hybrid from the locus classicus (SE Lithuania) were used for comparative morphological, anatomical and molecular analyses. The study proved the paternity of P. lucens and P. praelongus and excluded the involvement of P. gramineus in hybridization and identity with P. ×angustifolius. This hybrid formula is shared with plants recorded in the Czech Republic and Denmark under the name P. ×jutlandicus Zalewska-Galosz. The site in Lithuania, known since 1961, is the first natural habitat of long-lived hybrid between P. lucens and P. praelongus whose origin has been confirmed by molecular markers. The scarce records of this taxon indicate that the knowledge of the hybrid species of Potamogeton and their distribution is insufficient and that more extensive regional studies, including molecular methods, are needed.

The effect of natural herbicide from Fusarium equiseti crude extract on the aquatic weed water hyacinth (Eichornia crassipes (Mart.) Solms)

In this study, Fusarium equiseti was isolated from the weed plant Tridax procumbens in an agricultural field and a crude extract produced with 75% ethanol for use as active ingredient material in natural herbicides. The herbicidal effect of F. equiseti extract was tested on water hyacinth (Eichornia crassipes), an invasive aquatic weed, by leaf disk assay at concentrations of 0.05%, 0.1%, and 0.2% w/v crude extract. Dose-dependent visual toxicity symptoms were evident after three days, namely chlorosis, yellow leaves surrounded by dark brown edges. Photosynthetic pigments (chlorophyll a, b, and carotenoids) and membrane integrity (as electrolyte leakage and malondialdehyde content) were evaluated following the leaf disk test. 3 days after treatment, photosynthetic pigment contents showed dose-dependent decreases, while both measures of membrane integrity showed dose-dependent increases with increasing extract concentration. In addition, a cytogenetic assay was conducted on Allium cepa L. root, in which mitotic index reduction and depigmentation were evident as early as 24 h after herbicide application. Finally, anatomical analysis of treated E. crassipes leaves revealed degradation or damage of the ground tissue. All told, our results support the F. equiseti crude-based natural herbicide cloud as a sustainable alternative in agriculture.

Liana attachment to supports leads to profound changes in xylem anatomy and transcriptional profile of cambium and differentiating xylem.

Wood serves crucial functions in plants, yet our understanding of the mechanisms governing the composition, arrangement, and dimensions of its cells remains limited. The abrupt transition from nonlianescent to lianescent xylem in lianas represents an excellent model to address the underlying mechanisms, although consistent triggering factors for this process remain uncertain. In this study we examined how physical support attachment impacts the development of lianescent xylem in Bignonia magnifica (Bignoniaceae), employing a comprehensive approach integrating detailed anatomical analysis with gene expression profiling of cambium and differentiating xylem. Our findings demonstrate that attachment to physical supports triggers the formation of lianescent xylem, leading to increased vessel size, broader vessel distribution, reduced fibre content, and higher potential specific water conductivity than nonlianescent xylem. These shifts in wood anatomy coincide with the downregulation of genes associated with cell division and cell wall biosynthesis, and the upregulation of transcription factors, defense/cell death, and hormone-responsive genes in the lianescent xylem. Our findings provide insights into the regulation of xylem differentiation, driven by response to environmental stimuli. Additionally, they shed light on the mechanisms underlying the adaptation of lianas to climbing.

Three-dimensional anatomical analysis of the optic nerve canal with virtual model based on CBCT

The optic nerve canal (ONC) is a fine skeletal structure that contains the optic nerve. However, it has not been thoroughly examined. This necessitates establishing a baseline knowledge of the geometrical and volumetric parameters of the ONC. The data of twenty patients who received a cone beam computed tomography examination were prepared using a voxel-based segmentation. The measurement was performed separately by two examiners on virtual skull models in stereolithography format in Geomagic Wrap®. The results showed that the volume of the ONC varied between 92.48 mm3 and 162.7 mm3 (M = 123.46 mm3, SD = 26.61 mm3). Sex-specific statistically significant differences in volume were detected only for the right side. The angle of the ONC to the skull base was independent of the diameter of the canal. Both the intrarater and interrater comparisons of the measurements showed high values of reproducibility of the results. This study showed that a virtual anatomical model provides a feasible and reliable method to investigate the ONC. The examination technique could have a wider range of application in anthropology and application in clinical medicine. Advances in the automation of radiological diagnostics and the digital analysis of X-ray images could help to reduce examination times.

Scots pine roots (Pinus sylvestris L.) as dendrogeomorphological indicators of soil erosion on a hiking trail in the Brodnica Lakeland, Poland NE

Trampling on hiking trails leads to vegetation degradation, promoting soil erosion. Erosion removes the topsoil, causing lasting changes and exposing tree roots. Yet, little is known about the environmental factors affecting soil erosion rates determined from tree root analysis. This study, exclusively utilizing microscopic preparations, examines tree root anatomical changes on a lowland trail in Brodnica Lakeland. It aims to understand the temporal and spatial variability of soil erosion rates using dendrogeomorphological records of Scots pine (Pinus sylvestris L.) roots. Up to 64 % of samples show exposure due to continuous denudation, significantly shaping the trail’s relief. Abrupt exposures and secondary growth occur in only 22 % and 14 %, respectively. The average soil erosion rate, calculated from 76 root exposures at the Bachotek site, is 1.93 ± 0.82 mm/year, ranging from 0.71 to 3.80 mm/year, with recently exposed roots exhibiting the highest average soil erosion values on the hiking trail. 45 % of root samples exhibit above-average erosion rates. Statistical analyses reveal that erosion rates (ER) are influenced by soil compaction (COM), bulk density (BULK), root orientation (ASP), and exposure length (EXP). Higher compaction and bulk density correlate with increased erosion, while roots parallel to the trail experience more erosion. Conversely, greater root exposure length leads to decreased erosion rates. These findings suggest that soil erosion on the hiking trail primarily results from gradual geomorphological processes. Wood anatomical analyses of pine roots confirm the role of trampling in initiating and accelerating these processes, altering the trail’s subsurface soil layers, and indirectly affecting soil erosion dynamics. Scots pine roots prove valuable as indicators of the temporal and spatial variability of soil erosion along the lowland trail.

Enhancing the Horse Grimace Scale (HGS): Proposed updates and anatomical descriptors for pain assessment

The use of grimace scales enables the clinical identification of changes in the facial expressions of animals caused by pain. The Horse Grimace Scale (HGS) is one such tool, comprising a pain coding system based on facial expressions and assessing six Facial Action Units (FAUs). Each FAU is accompanied by descriptions and anatomical details to assist the evaluator. However, the morphological descriptions for certain FAUs in the HGS are not sufficiently detailed, potentially hindering accurate interpretation. This study is an analytical investigation aimed at enhancing the morphoanatomical details in the HGS and providing raters with more comprehensive materials for pain evaluation in horses using this scale.To achieve this, detailed anatomical analyses were conducted using established references in veterinary anatomy. Initially, we propose substituting the term 'ear' with 'auricle' or 'pinna' and replacing 'area above the eye' with 'supraorbital region' for anatomical accuracy. Additionally, we introduce detailed morphoanatomical descriptions that identify specific landmarks, with the goal of ensuring more consistent application of the HGS and reducing interpretation variability. Furthermore, this study provides an explanation of the muscles involved in the investigated FAUs. These adjustments on the descriptions and evaluations remain unverified, however it is anticipated that the descriptive enhancements lead us to understand that higher interobserver reliability can be achieved for each of the FAUs.

Anatomical and electrophysiological analysis of the fasciola cinerea of the mouse hippocampus.

The hippocampus is considered essential for several forms of declarative memory, including spatial and social memory. Despite the extensive research of the classic subfields of the hippocampus, the fasciola cinerea (FC)-a medially located structure within the hippocampal formation-has remained largely unexplored. In the present study, we performed a morpho-functional characterization of principal neurons in the mouse FC. Using in vivo juxtacellular recording of single neurons, we found that FC neurons are distinct from neighboring CA1 pyramidal cells, both morphologically and electrophysiologically. Specifically, FC neurons displayed non-pyramidal morphology and granule cell-like apical dendrites. Compared to neighboring CA1 pyramidal neurons, FC neurons exhibited more regular in vivo firing patterns and a lower tendency to fire spikes at short interspike intervals. Furthermore, tracing experiments revealed that the FC receives inputs from the lateral but not the medial entorhinal cortex and CA3, and it provides a major intra-hippocampal projection to the septal CA2 and sparser inputs to the distal CA1. Overall, our results indicate that the FC is a morphologically and electrophysiologically distinct subfield of the hippocampal formation; given the established role of CA2 in social memory and seizure initiation, the unique efferent intra-hippocampal connectivity of the FC points to possible roles in social cognition and temporal lobe epilepsy.

STUDY ON ANATOMICAL CHARACTERIZATIONOF RHINACANTHUS NASUTUS (L.) KURZ

Rhinacanthus nasutus (L.) Kurz, belonging to the family Acanthaceae, is an herbaceous shrub. This plant holds significant value in traditional medicine. The leaves of R. nasutus are commercially available as tea bags, marketed as a natural remedy for various ailments. However, the conservation status of R. nasutus necessitates appropriate protective measures to ensure its sustainability. Anatomical studies provide various histological layers, including the epidermis, cortex, vascular bundles, and pith. The leaves feature numerous diacytic stomata and hypostomatic nature. The leaf midrib exhibits a narrow epidermis with numerous glandular hairs. The medulla contains arc-shaped vascular bundles and lateral accessory bundles, with the stele comprising linear xylem elements towards the inner side and phloem elements on the outer region. Transfusion tracheids are present between the palisade and spongy cells, with tissues containing large quantities of oil droplets. The petiole shows a narrow epidermis with numerous glandular hairs. The vascular region features a V-shaped stele. The stem reveals the stelar region is extensive and the inner region is rich in developed xylem elements with linear multiple vessels. The medullary ray cells are narrow, and the pith region displays intruded primary xylem with thick-walled parenchyma cells. Wood anatomical analysis shows xylem parenchyma cells that are paratracheal. The vessel elements possessing perforation plates and transverse walls with bordered pits. Tracheids have a narrow lumen, and the lateral walls contain simple pits. The wood demonstrates multiseriate ray parenchyma and the bark consists of phloem rays and narrow fibers. In conclusion, R. nasutus exhibits a variety of histological and anatomical characteristics that support its traditional medicinal uses. The detailed anatomical studies contribute to understanding the plants structural adaptations and potential environmental interactions, thereby facilitating its identification and utilization in herbal medicine. The conservation of this species is critical to preserving its medicinal value and ensuring its availability for future research and therapeutic applications.

Comparative anatomical characteristics of the distal parts of bear and human limbs

BACKGROUND: The comparative morphology of the human and animal skeletons has been incompletely described in the forensic medical and anthropological literature. Moreover, bones of the distal parts of bear limbs are anatomically similar to those of humans. Together with some features of the bear’s skeleton, poor preservation, absence of claws, and incomplete remains, difficulties and errors may occur during identification. AIM: To create an illustrative material describing morphological features important for the identification of each element of the distal parts of bear limbs in comparison with humans. MATERIALS AND METHODS: Preparations of the distal parts of the right thoracic and right pelvic extremities of the bear were made using osteological materials. The missing talon phalanges of a bear and bones of a human hand and foot were taken from the institute collections. The International Veterinary Anatomical Nomenclature was used to describe the anatomical features of bear bones, and the latest recommendations of International Anatomical Terminology were considered for human bones. RESULTS: Each bone of the bear’s hand and foot was described in comparison with a similar human bone. For greater versatility, descriptions were made in terms of the international zoological nomenclature. For all bones, except for distal sesamoid bones, high-quality photos are provided for aspects that are important for identification. Comparative anatomical analysis showed that the bones of the wrist differ to a greater extent, whereas all tarsal bones, which are part of the human foot, had analogs in the bear foot and had closer measurements. The articular surfaces of the heads of the metacarpals and metatarsals showed specific ridges articulating with the cutouts at the bases of the proximal phalanges of the fingers. In addition, the bear’s hand and foot contained numerous inset sesamoid bones and claw-like processes on the distal phalanges of the fingers. CONCLUSION: Comparative anatomical analysis showed similarities in the structures of the bones of the hand and foot of a brown bear and a human caused by foot walking. Owing to the morphological similarity, bone identification can be difficult. The set of features described in the article, which are specific to bear bones, in combination with illustrative material will help in identifying bones more accurately, even for individual bones.

Ma fermeture d'auricule s'est compliquée : traitement percutané d'une rupture d'auricule gauche

An 81-year-old patient was referred for left atrial appendage closure. Anatomical LAA analysis by CT scan showed an inverted chicken wing morphology. The procedure was performed through i an infero-anterior transseptal puncture and led to “sandwich” closure strategy using an AMPLATZER AMULET 25 mm device. Despite successful deployment of the occluder, a hemopericardium soon developed related to an iatrogenic LAA perforation/partial rupture and leading to major hemodynamic instability. After pericardocentesis, it was decided to inject activated thrombin into the pericardial sac to achieve in situ hemostasis. This strategy enabled coagulation of the hemopericardium and cessation of active bleeding, without recourse to surgical treatment.

CT Anatomical Analysis of C4 Pedicle and Lateral Mass in Children Aged 0-14 in Southern China.

The C4 is the transition point between the upper and lower cervical vertebrae and plays a pivotal role in the middle of the cervical spine. Currently, there are limited reports on large-scale sample studies regarding C4 anatomy in children, and a scarcity of experience exists in pediatric cervical spine surgery. The current study addresses the dearth of anatomical measurements of the C4 vertebral arch and lateral mass in a substantial sample of children. This study aims to measure the imaging anatomy of the C4 vertebral arch and lateral mass in children under 14 years of age across various age groups, investigate the growth and development of these structures. We measured 12 indicators, including the size (D1, D2, D3, D4, D5, D6, D7, and D8) and angle (A, C, D, and E) of the C4 vertebral arch and lateral mass, in 513 children who underwent cervical CT examinations at our hospital. We employed the aggregate function for statistical analysis, conducted t-tests for difference statistics, and utilized the least squares method for regression analysis. Overall, as age increased, there was a gradual increase in the size of the vertebral arch and lateral mass. Additionally, the medial inclination angle of the vertebral arch decreased, and the lateral mass flattened gradually. The rate of change decreased gradually with age. The mean value of D1 increased from 2.31 mm to 3.88 mm, of D2 from 16.75 mm to 29.2 mm, of D3 from 2.21 mm to 4.92 mm, and of D4 from 7.34 mm to 11.84 mm. Meanwhile, the mean value of D5 increased from 5.2 mm to 9.71 mm, of D6 from 10.19 mm to 16.16 mm, of D7 from 2.53 mm to 5.67 mm, and of D8 from 6.11 mm to 11.45 mm. Angle A ranged from 49.12° to 54.97°, angle C from 15.28° to 19.83°, angle D from 39.91° to 53.7°, and angle E from 18.63° to 28.08°. Prior to cervical spine surgery in children, meticulous CT imaging anatomical measurements is essential. The imaging data serves as a reference for posterior C4 internal fixation, aids in designing posterior cervical screws for pediatric patients, and offer morphological anatomical references for posterior cervical spine surgery and screw design in pediatric patients.