In this paper, a novel 3D metrology method using one camera with rotating anamorphic lenses is presented based on the characteristics of double optical centers for anamorphic imaging. When the anamorphic lens rotates -90° around its optical axis, the 3D data of the measured object can be reconstructed from the two anamorphic images captured before and after the anamorphic rotation. The anamorphic lens imaging model and a polynomial anamorphic distortion model are firstly proposed. Then, a 3D reconstruction model using one camera with rotating anamorphic lenses is presented. Experiments were carried out to validate the proposed method and evaluate its measurement accuracy. Compared with stereo vision, the main advantage of the proposed 3D metrology approach is the simplicity of point matching, which makes it suitable for developing compact sensors for fast 3D measurements, such as car navigation applications.