Analysis Of Plant Material Research Articles

Rapid preparation of plant samples for cation analysis

Abstract In the dry ashing of plant material for chemical analysis, the hot digestion on the steam bath of the ashed sample was replaced by the quantitative addition of dilute HCl and stirring very briefly to dissolve the ash. By waiting a few minutes for the particles to settle, there was no need to filter the solution prior to dilution with an automatic diluter and the determination of K, Na, Ca and Mg by atomic absorption spectrophotometry. The new procedure permits the handling of large batches of samples conveniently, eliminates the use of pipettes and volumetric flasks and decreases the risk of inadvertent contamination by sodium and therefore enhances accuracy. Precision was at least as good as with the standard procedure and the method is expected to be more suitable for use in conjunction with automated analytical techniques.

Electron probe analysis of freeze-substituted, epoxy resin embedded tissue for ion transport studies in plants

A new technique is described to prepare plant material for electron probe analysis. Root segments 1 mm in length were frozen at-170°, freeze-substituted with anhydrous ether at-30° and infiltrated with Spurr's low-viscosity epoxy resin embedding medium at low temperatures. Sections 1 and 2μ thick were cut anhydrously using hexylene glycol in the ultramicrotome trough, mounted on the polished surface of a Be disc and vacuum coated with 150-200 Å aluminum.The new technique allows retention of water-soluble ions at the original sites in the tissue and is superior to cryostat sectioning in spatial resolution of electron probe analysis and in the preservation of cellular structures.The lateral transport of K(+) into the xylem of corn roots has been successfully studied by electron probe analysis of freeze-substituted, epoxy resin embedded material.

Removal of humin in hydrolyzates of plant material for amino acid analysis

Removal of humin in hydrolyzates of plant material for amino acid analysis

Preparation of Plant Material for Analysis

Abstract A survey was made of the problems, practices, and apparatus involved in the preparation of plant material for analysis. This report summarizes the replies to a questionnaire sent to 200 investigators concerned with plant analysis.

The effect of phosphorus, potassium, and calcium on the growth, chemical composition, and symptoms of deficiency of white clover in a subtropical environment

The effect of the rates of application of sodium phosphate, potassium chloride, and calcium carbonate on growth, chemical composition, and visual symptoms of deficiency in Trifolium repens var. Irrigation White, grown in a low humic gley soil under subtropical conditions in Queensland, has been investigated. The surface soil is grossly deficient in phosphorus, potassium, and calcium and consists almost entirely of sand (92 per cent.). Additions of sodium phosphate, potassium chloride, and calcium carbonate each increased the yield of clover, increased the phosphorus, potassium, and calcium contents respectively, and conditioned the uptake of other nutrients. Plant material for chemical analyses consisted of petioles and leaves (less senescent and expanding leaves), harvested at the immediate pre-flowering stage of growth. Critical percentages for phosphorus, potassium, and calcium under these conditions were established at 0.23, 1.1, and 1.0 per cent. respectively. Visual symptoms of phosphorus, potassium, and calcium deficiencies are discussed in relation to yield and nutrient content. The sum of the equivalents of potassium, calcium, and magnesium is approximately constant. The mean value of 18 analyses covering all treatments was 136 m-equiv. per cent. with a range of 116-148.

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Report on Preparation of Plant Material for Analysis

Journal Article Report on Preparation of Plant Material for Analysis Get access H R Kraybill H R Kraybill Associate Referee Agricultural Experiment Station, Purdue, Ind Search for other works by this author on: Oxford Academic Google Scholar Journal of Association of Official Agricultural Chemists, Volume 11, Issue 2, 15 May 1928, Pages 208–209, https://doi.org/10.1093/jaoac/11.2.208 Published: 24 February 2020