The Tamera open pit mine is located in the Nefza mining district (north-west of Tunisia) where the reddish soil reveals its strong potential for heavy Fe mineralization, along with other metals (Pb, Zn, and Mn). This study explored Iron oxide mineralization in a particular nappe zone using multiscale field visible and near infrared/short waves infrared and EO-1 Hyperion hyperspectral data. Thus, spectral features fittings were conducted on Hyperion data for mineral mapping using scene-extracted endmembers. A first minimum noise fraction algorithm was used to reduce the hyperspectral data’s dimensionality, followed by the Pixel Purity Index and n-dimensional visualization to extract the pure pixels (endmembers) from the cluster of pixels in the data cube. Furthermore, 56 soil samples were collected from the test site and spectrally measured using Analytical Spectral Devices FieldSpecHiRes spectrometer, which covers the spectral range between 350 and 2500 nm. All samples were analyzed in the laboratory with x-ray diffraction. Endmember extracted spectra were compared with field measurements before EO-1 Hyperion processing. Mapping results revealed the occurrence of a mineralogy composition consisting particularly of iron oxide minerals (hematite and goethite) and bearing minerals, such as clays (kaolinite), within all samples.