Digitalisation is shaping the contemporary technological context of entrepreneurial activities, where firms grow through interacting with digital ecosystem stakeholders. This study investigates how incumbent firms seek entrepreneurial growth by re-configurating their knowledge bases in digital business ecosystems. We propose and develop a conceptual framework that blends the digital business ecosystem perspective and the knowledge-based view of the firm. Through a longitudinal case study of a Chinese textile manufacturing firm, we identify three pathways for entrepreneurial growth. The results contribute to the entrepreneurship literature by demonstrating how digital technologies foster corporate entrepreneurship in incumbent firms. The proposed framework extends the analytical power of the knowledge-based view by incorporating ecosystem elements into the firm’s internal and external knowledge management. The findings also generate relevant and actionable managerial implications for entrepreneurs, managers, and policymakers that are applicable in the context of digital business ecosystems.