Analytical Analysis Research Articles

Correction: Muniyappan et al. Stability and Numerical Solutions of Second Wave Mathematical Modeling on COVID-19 and Omicron Outbreak Strategy of Pandemic: Analytical and Error Analysis of Approximate Series Solutions by Using HPM. Mathematics 2022, 10, 343

In the original publication [...]

Green and Reflux method synthesis of CeO2/rGO for their characterization and Photodegradation of dye

In this study, cerium oxide (CeO2) was synthesized using a green and eco-friendly solution combustion method with lemongrass as the fuel source. The synthesis process was simple and environmentally friendly, leveraging a straightforward reflux technique to prepare the CeO2/rGO composite. The resulting CeO2 and CeO2/rGO composite was characterized using various analytical techniques, including XRD, FE-SEM, FTIR, EDX, UV-Vis, XPS, and BET analysis. The photocatalytic performance of the CeO2/rGO composite was evaluated through the degradation of Methyl Violet (MV) dye, demonstrating a remarkable photocatalytic efficiency with approximately 99 % degradation following a first-order reaction kinetics. The half-life period (t₁/₂) of the degradation process was determined to be 19.01 minutes, and the rate constant (k) was calculated to be 0.03971 min⁻¹. The study also explored various factors affecting the photocatalytic activity, including pH levels, dye concentration, light source, and the amount of catalyst used. Additionally, scavenger studies were performed to identify the reactive species involved, and the total organic carbon (TOC) removal efficiency was evaluated. The reusability of the CeO2/rGO catalyst was also investigated, demonstrating its potential for sustainable and effective application in environmental remediation processes.

Mechanical characteristics and structural performance of rubberized concrete: Experimental and analytical analysis

Rubberized concrete is referred to as "green concrete" by replacing fine aggregate with used tire rubber. However, although significantly increasing ductility, rubber particles lowered compressive strength. The study aimed to find the optimum ratio of crumb rubber (C-Ru) to employ in large scale elements. Firstly, the effect of C-Ru treatment and content on concrete mechanical behavior is examined. The experimental program contains 96 cubes and 78 cylinders using different percentages of untreated/treated C-Ru with portions from 5 % to 30 % replacing the sand. Then, four beams were produced with 5, 10, and 15 % sand volume substitution by C-Ru to investigate the effects of rubber content on structural performance and compared with conventional beam. It is found that C-Ru treatment has less enhancement for concrete fresh properties (workability) for using small ratios of rubber and this enhancement increases to reach 20 % for higher rubber content ratio while the comparable enhancement for hardened properties (compressive strength). In addition, well washing of the C-Ru after treatment did not affect the fresh RuC properties, while the improved the hardened properties of RuC (compressive and tensile strengths). Increasing the C-Ru content ratio decreased concrete fresh and hardened properties. Untreated C-Ru not more than 15 % as a replacement of fine aggregates is preferred to avoid large strength reductions while benefiting workability, economy, and safety. While the addition of C-Ru to the concrete mix increased the ductility, toughness, and self-weight of the beams, it also decreased their flexural capacity. Finally, the finite element analysis using ANSYS is used to compare with experimental results and thus it could accurately model behavior of rubberized concrete as a complex material that recommended for further studies.

Experimental and Analytical Evaluation to Strengthened R.C Beams Using Ferrocement Under Torsion

The safety of structures around the globe represents a significant and pressing concern, underscoring the urgent need for the development and implementation of effective rehabilitation methods to ensure their continued functionality. Ferrocement has emerged as a durable method for the restoration of reinforced concrete (RC) structures, demonstrating resilience and versatility in addressing structural damage. A substantial body of research has been conducted to examine the mechanical properties of ferrocement, with a particular focus on its efficacy in reinforcing beams against a range of forces. In light of the limited understanding of the factors affecting the torsional strength of ferrocement-wrapped beams, it is imperative to conduct experimental investigations. The objective of this study is to evaluate and compare the torsional strength, angle of twist and crack development of different wrapping types using both analytical analysis and experimental data. The findings indicate that three-sided wrapping is an effective method for enhancing torsional behaviour, as evidenced by both experimental and analytical results. Furthermore, the utilization of styrene-butadiene rubber (SBR) as a connecting material has been demonstrated to be an effective method for maintaining cohesion across reinforced beams.

Global buckling response of sandwich panels with additively manufactured lattice cores

This paper deals with the analytical and numerical global buckling analysis of rectangular sandwich plates utilized AlSi10 Mg in both facesheets and lattice cores. In this study, six different strut-based lattice core models are designed. Additionally, the buckling resistance of lattice panels is compared with a typical honeycomb panel. Analytical studies were carried out using Kirchoff plate theory (CLPT), first-order shear deformation theory (FSDT) coded on MATLAB and finite element (FE) analyses in Abaqus. In the theoretical approaches, the Navier solution is derived for sandwich plates with simply supported boundary conditions at all edges. In the FE analyses, validated homogenized lattice structures models were used to avoid excessive numbers of elements and to save computational time. The parametric effects of side-to-thickness ratio, and different designed core cells on global buckling responses are investigated. As a result of comparing the analytical results with the FE model, a good agreement is obtained, and it is found that analytical buckling analyses FSDT can be used within certain size limits for the global buckling analysis of lattice core sandwich structures.

Ukrainian guidelines for the prevention and treatment of glucocorticoid-induced osteoporosis

Introduction. Glucocorticoid-induced osteoporosis (GIOP) is a bone metabolic disease that develops as a result of glucocorticoid (GC) use, is one of the most frequent causes of secondary osteoporosis, leads to an increase in the risk of low-energy fractures, disability and mortality of GC users. Despite the few stu­dies conducted in Ukraine on the epidemiology and treatment of GIOP, there have been no national recommendations for its ma­nagement until now, which has become the background for their creation. The purpose was to develop national guidelines for the diagnosis, prevention, and treatment of GIOP based on the analytical analysis of modern literary sources to improve the awareness of the medical community of Ukraine, improve management, and reduce the socio-economic burden of the disease. Materials and ­methods. A group of 18 experts, leading Ukrainian scientists in various fields, was created to develop the guideline. An analysis of current literary sources on epidemiology, risk factors, diagnosis, prevention and treatment of GIOP, and monitoring of the effectiveness and safety of its treatment was carried out. Evidence was synthesized ­using the GRADE system, and a critical assessment of the quality of the recommendations was carried out using the AGREE II tool. ­Results. The guideline consists of 12 provisions on screening, diagnosis, prevention, and treatment of GIOP. The authors emphasized the need to increase the awareness of the medical community of Ukraine regarding the problem, as well as patients regarding the possible side effects of GC. They presented the features of the clinical assessment of the osteoporotic fracture risk in GC users, as well as modern and available in Ukraine methods of diagnosis and treatment of GIOP. Conclusion. The first Ukrainian guideline for screening, diagnosis, prevention, and treatment of GIOP is an important national tool for its management, which is recommended by the Board of the Ukrainian Association of Osteoporosis for use in practical health care by doctors of various specialties.

Design and Modeling of a Long Range Motion Compliant Nanopositioning Stage Driven by a Normal Stressed Electromagnetic Actuator

Compliant nanopositioning stages with built-in ultra-precision actuators are frequently integrated into production and analysis instruments comprising ultra-high precision motion generation systems. These stages are essential nanotechnology and advanced material analysis components, providing precise positioning capabilities for various applications. However, in the practical engineering field, there is a lack of compliant nanopositioning stages that can achieve a long-range motion while maintaining accuracy, reliability, and compact size, which is the inspiration for this research. This paper investigates the design, modeling, and experimental testing of a long-range motion-compliant nanopositioning stage driven by a normal stressed electromagnetic actuator (NSEA). The nanopositioning stage components’ structural framework and working principle, including NSEA, bridge type distributed compliant (BTDC) mechanism, and the guiding mechanism, are fully examined to derive an analytical model. The analytical model is utilized in the sections that follow. Factors affecting the stroke and natural frequency of the nanopositioning stage are also illustrated. The optimization process of the nanopositioning stage is conducted in pursuit of a high-precision stage by specifically looking into the electromagnetic, BTDC mechanism, and guiding mechanism parameters. This optimization procedure also takes into account various design constraints, including stiffness, saturation flux density, and stress. Furthermore, the finite element analysis is used to verify the analytical model, and the results are discussed. The prototype is fabricated with reference to the analytical and finite element analysis results, and the experimental tests are conducted, including motion and natural frequency tests. In addition, a control system, which adopts both a proportional-integral-derivative controller and a damping controller, is designed to create a closed-loop system. Finally, the tracking performance of the stage was investigated, and a very minimal tracking error was observed. Overall, the comprehensive models and experimental tests proved the stage to be a good model which achieved the objective of the research.

Economic Implications and Public Readiness for Urban Green Space Development in Agra: A Strategic Evaluation in the City of Taj

Urban green spaces (UGSs) improve the environmental value of the city as well as the quality of life for citizens. Unfortunately, many cities in India are lagging behind the minimum standard of UGS required in the city. For example, Agra city in India, one of the most famous tourist destinations in the world, represents two distinct realities of UGS in the same city. One is the focus of tourism, and the rest is the place of common people with very limited public green spaces, leading to multiple social issues (like spatial polarization, etc.). The secondary data concludes that the PPGC of Agra is lower than the standards, and the ongoing schemes are approached through quantitative methods. Despite having many UGS development schemes, Agra's UGS shows stalled growth. This research examines the policies and programs of UGS development (AMRUT, SCM, etc.) and reviews them to understand the unique gaps and possible regulatory interventions. The study includes an assessment of stakeholders' readiness to accept plausible UGS strategies using an analytical analysis approach. The primary data shows that PPP is the requirement for the integrated development of UGSs. The planners can make policies highlighting citizen's rights and responsibilities to enhance UGSs in Agra.

Coupling effect of hole enlargement and diffusion in grouting process in weak stratum based on analytical analysis

Coupling effect of hole enlargement and diffusion in grouting process in weak stratum based on analytical analysis

Статистические аспекты обращений детей за стоматологической помощью при острой зубной боли.

The study analyzes the characteristics of the treatment of children with acute toothache in dental departments. The object of the study was the medical records of dental institutions in St. Petersburg. The subject of the study is the treatment of children of different age groups. The time period of the study is from 2010 to 2023. Relevance. The study of pediatric dentistry issues related to intense toothache occupies a special place in many studies. This is largely due to the age characteristics of children, who, due to their lack of development of communication skills, are not able to independently control what is happening. Analyzing applications to dental clinics, special attention should be paid to the fact that a significant part of patients are children suffering from acute toothache. This phenomenon highlights the importance of a statistical study of the causes and sequences associated with the need for dental care among the younger generation. Focusing on aspects related to children’s dental care for acute toothaches opens up access to a deep understanding of the problem and the development of strategies to overcome it. The study of statistical data can reveal certain trends, which, in turn, will help to develop more effective methods of prevention and treatment of dental diseases in children. The purpose of the study: the study of statistical aspects of children’s requests for dental care for acute toothache. Materials and methods of research: analysis of scientific and practical literature, generalization, statistical and analytical analysis. As a result of the research, a review of scientific, practical, statistical information was carried out, an empirical study was conducted, and the author’s conclusions were given. Based on the analysis, which affected the frequency of children’s visits to dental institutions, the main reasons for such visits were identified. At the top of the list are preventive measures that emphasize parents’ awareness of the importance of taking care of their child’s dental health, as well as indicating the effectiveness of educational programs in the field of dentistry. They are followed by an urgent need to consult a specialist due to severe toothache caused by a variety of causes, among which there may be diseases such as pulpitis or periodontitis. The analysis of data for the period from 2010 to 2023 revealed the immutability of the main reasons for dental office attendance and the quantitative characteristics of such requests, which confirms the constant relevance of this issue

Innovate! Accelerate! Evaluate! Harnessing the RE-AIM framework to examine the global dissemination of parenting resources during COVID-19 to more than 210 million people

BackgroundParents were at the forefront of responding to the needs of children during the COVID-19 pandemic. This study used the RE-AIM framework to examine the Reach, Effectiveness, Adoption, Implementation, and Maintenance of a global inter-agency initiative that adapted evidence-based parenting programs to provide immediate support to parents.MethodsData were collected via short surveys sent via email, online surveys, and analysis of social media metrics and Google Analytics. Retrospective surveys with 1,303 parents and caregivers in 11 countries examined impacts of the resources on child maltreatment, positive relationship building, parenting efficacy, and parenting stress.ResultsThe parenting resources were translated into over 135 languages and dialects; reached an estimated minimum 212.4 million people by June 2022; were adopted by 697 agencies, organizations, and individuals; and were included in 43 national government COVID-19 responses. Dissemination via social media had the highest reach (n = 144,202,170, 67.9%), followed by radio broadcasts (n = 32,298,525, 15.2%), text messages (n = 13,565,780, 6.4%), and caseworker phone calls or visits (n = 8,074,787, 3.8%). Retrospective surveys showed increased parental engagement and play, parenting self-efficacy, confidence in protecting children from sexual abuse, and capacity to cope with stress, as well as decreased physical and emotional abuse. Forty-four organizations who responded to follow-up surveys in April 2021 reported sustained use of the resources as part of existing services and other crisis responses.ConclusionThis study highlights the importance of a) establishing an international collaboration to rapidly adapt and disseminate evidence-based content into easily accessible resources that are relevant to the needs of parents; b) creating open-source and agile delivery models that are responsive to local contexts and receptive to further adaptation; and c) using the best methods available to evaluate a rapidly deployed global emergency response in real-time. Further research is recommended to empirically establish the evidence of effectiveness and maintenance of these parenting innovations.

Analytical Sensitivity Analysis and Clinical Impact Modeling of Rapigen Rapid Diagnostic Tests for Malaria.

Laboratory benchmarking allows objective analysis of the analytical performance of malaria rapid diagnostic tests (RDTs). We present the analytical detection limits of the Rapigen BIOCREDIT Malaria Ag Pf/Pv (pLDH/pLDH), the Rapigen BIOCREDIT Malaria Ag Pf (pLDH/HRPII), and two best-in-class WHO-prequalified comparator RDTs, generated using standardized panels containing recombinant antigen, invitro cultured parasites, international standards, and clinical samples. Detection limit antigen concentrations of HRP2, PfLDH, and PvLDH were determined for the Rapigen and comparator RDTs. Detection of antigens in international units (IU)/mL was also evaluated. The Rapigen Ag Pf (pLDH/HRPII) detected 3.9 and 3.9 IU/mL for PfLDH and HRP2, respectively, and the Ag Pf/Pv (pLDH/pLDH) detected 3.9 and 5.0 IU/mL for PfLDH and PvLDH, respectively. The comparator HRP2/PfLDH and HRP2/PvLDH detected 15.6 and 31.3 IU/mL for HRP2 and PfLDH and 15.6 and 50.0 IU/mL for HRP2 and PvLDH, respectively. The RDT clinical sensitivity was predicted through application of analytical detection limits to antigen concentration distributions from clinical symptomatic and asymptomatic cases. Febrile cases would be detected in a majority by both standard and Rapigen RDTs, but incremental increases in sensitivity in the Rapigen RDTs may be important for clinical cases currently missed by microscopy. Rapigen RDTs were predicted to have improved detection of asymptomatic cases and infections with parasites carrying hrp2 deletions through more sensitive PfLDH detection. Through the benchmarking and simulation of clinical sensitivity, a method for rapidly assessing the ability of new RDTs to meet clinical needs using high-sensitivity antigen distribution data is presented.

The relationship between nutritional status and thyroid function among adults in the USA: NHANES 2007-2012.

Controlled Nutritional Status (CONUT) scores have been developed as quantitative tools that can be employed to gauge the nutritional status of individual patients. However, there has been very little research investigating the association between these CONUT scores and the function of the thyroid. As such, the present study was designed to address this research gap through the evaluation of a representative cohort of American adults. National Health and Nutrition Examination Survey (NHANES) data were herein used to separate subjects into those with normal nutritional status (CONUT score: 0-1) from those who were malnourished (CONUT scores> 1). Associations between these CONUT scores and the function of the thyroid were investigated through linear regression modeling, employing weighted analytical strategies and subgroup analyses. Overall, 8082 individuals from the NHANES 2007-2012 cohort were enrolled in this analysis. The weighted mean CONUT score for these individuals was 0.72 (0.02), with 6661 participants (weighted proportion: 83.12%) falling within the normal nutritional status group and 1421 participants (weighted proportion: 16.88%) within the malnourished group. In adjusted analyses, subjects who were malnourished were found to present with an increase in FT4 levels (β = 0.033; p<0.001 together with reduced TT3 levels (β = -3.526; p=0.01). The present data offer evidence in support of higher CONUT scores, which correspond to malnutrition, being related to increases in FT4 levels together with reductions in TT3 levels. More studies will be crucial to further probe the mechanistic drivers of these results.

A “poor-man’s” deformation plasticity based approach to topology optimization of elastoplastic structures

This paper presents a topology optimization framework utilizing a deformation plasticity model to approximate the isotropic hardening von-Mises incremental elastoplasticity model under monotone proportional loading. One advantage of the model is that it is based on a yield surface allowing for precise matching to uniaxial elastoplastic isotropic hardening response. The deformation plasticity model and the incremental plasticity model coincides for proportional loading and since the deformation plasticity model is path-independent, the computational cost and implementation complexity reduce significantly compared to the conventional incremental elastoplasticity. To investigate the deformation plasticity model combined with topology optimization, we compare three common elastoplastic optimization objectives: stiffness, strain energy and plastic work. The possibility to limit the peak local plastic work while maximizing the strain energy is also investigated. The consistent analytical sensitivity analysis which only requires the terminal state is derived using adjoint method. Numerical examples demonstrate that the proportionality assumption is reasonable and the deformation plasticity model combined with topology optimization is a competitive alternative to cumbersome incremental elastoplasticity.

Módulo de promoción del uso responsable de antimicrobianos en odontología: diseño instruccional guiado por la teoría de carga cognitiva

IntroductionAproximately 30% to 50% of annual antimicrobial prescriptions are inappropriate or unnecessary, enhancing the development of bacterial resistance. It has been proposed that the responsible use of antimicrobials in undergraduate programs be emphasized. However, teachers usually do not manage effective strategies for this purpose. Aims1. Develop an instructional design, guided by the cognitive load theory, of a teaching and learning module to promote responsible use of antimicrobials 2. Evaluate the educational impact of the module on third-year Dental students. MethodsAction research and mixed methodology in four phases. i. Identification of learning needs and outcomes; ii. Instructional design guided by cognitive load theory; iii. Module implementation; iv. Educational impact on students was evaluated using satisfaction surveys, self-reports of learning outcomes achievement and a pre-post knowledge test. Descriptive and analytical statistical analysis (Wilcoxon test) and qualitative content analysis were used. ResultsThe antimicrobial module was designed in Canvas (LMS), allocating protected time for study and a classroom session. The educational impact was evaluated in 48 students. The satisfaction survey reported positive results on their 10 items (minimum 81.3%). The results of the self-reports of achievement of 7 learning outcomes and the pre-post test of 18 questions were statistically significant (p<0.001). ConclusionsThe design of this module could serve as a guide to promote the responsible use of antimicrobials while achieving greater satisfaction with students’ experience and learning outcomes.

Chronic Kidney Disease-Associated Pruritus Burden: A Patient Survey Study

Rationale & ObjectiveChronic kidney disease–associated pruritus (CKD-aP) is a common, underrecognized condition in patients with chronic kidney disease (CKD), especially those receiving hemodialysis (HD). The present study analyzed the clinical treatment journey and overall burden of pruritus among patients with CKD-aP. Study DesignCross-sectional, patient-reported online survey. Setting & ParticipantsData from adult patients undergoing HD (December 2021-May 2022) in the United States. ExposurePatients participated in an online survey and responded to questions on validated patient-reported outcome (PRO) instruments related to CKD-aP. OutcomesSelf-reported measures analyzed at the end of this survey include itch characteristics, symptom management, healthcare provider (HCP) engagement, and impact on HD, quality of life (QoL), sleep, and work productivity. Analytical ApproachBivariate analysis assessed the association of itch severity with CKD-specific QoL. ResultsOverall, 354 patients with CKD-aP were included in analyses, of which 49% and 30% had moderate and severe itch, respectively (22% were mild). Around 68% reported symptoms to HCPs, most commonly a nephrologist or primary care provider, and 55% received a treatment recommendation. The most common treatments were topical lotions/moisturizers (75%) and corticosteroids (48%); use of oral prescriptions was low (20%), with limited satisfaction with treatments. Overall, 23% of patients reported shortening and 17% reported missing HD sessions due to itch. In bivariate analysis, patients with more severe CKD-aP reported significantly worse disease and function scores [kidney disease (KD) score, cognitive function, quality of social interaction, sleep (all, P<0.001), and sexual function (P<0.05)], suggesting a direct impact of CKD-aP on QoL. LimitationsPossible recall bias, especially for questions with longer recall periods. ConclusionsCKD-aP is often inadequately treated, and disruptive of dialysis treatment, even among patients who report itch to HCPs. Worse itch severity is associated with poorer QoL, sleep quality, and functional/work impairment.

Analytical and Numerical Analysis of Circumbinary Disk Dynamics. I. Coplanar Systems

We present an analytical and numerical study of a system composed of a stellar binary pair and a massless, locally isothermal viscous accretion disk that is coplanar to the binary orbital plane. Analytically, we study the effect of the binary’s gravitational potential over short timescales through the stability of epicyclic orbits, and over long timescales by revisiting the concept of resonant torques. Numerically, we perform two-dimensional Newtonian simulations of the disk-binary system over a range of binary mass ratios. We find that the results of our simulations are consistent with those of previous numerical studies. We additionally show, by comparison of the analytical and numerical results, that the circumbinary gap is maintained on the orbital timescale through the driving of epicyclic instabilities, and does not depend on resonant torquing, contrary to the standard lore. While our results are applicable to any disk-binary system, we highlight the importance of this result in the search for electromagnetic and gravitational-wave signatures from supermassive black hole binaries.

Design, analytical and computational analysis, and development of a high-precision CNC spindle for a vertical machining center

This research focuses on design, simulation and development of a high precision Computer Numerically Controlled (CNC) spindle for vertical machining center. High speed spindle is designed and analyzed under the varying load conditions. SolidWorks 2020 is used for designing of CNC spindle (based on ISO BT40 standards) and ANSYS Workbench 2020 is used for analysis. Moreover, analytical calculations for deformation are also done. Three different materials i.e. AISI 4340 steel, Grey cast iron, and Ti6Al4V alloy are chosen for the analysis. Effect of varying load on deformation and stress on CNC spindle is discussed. AISI 4340 steel showed better mechanical characteristics as compared to other materials. Moreover, the results showed that steel gives lesser values of deformation as compared to other materials, when applied under different load conditions. Although Grey cast iron can be economical and weight of the spindle can also be reduced but Grey Cast iron has greater value of deformation when load is applied. On the other hand, Ti6Al4V alloy specimen exhibited less stress as compared to other materials but mechanical properties in terms of modulus of elasticity are not that good as AISI 4340 steel. So, AISI 4340 steel could be a good option for spindle materials that require desired properties such as strong corrosion resistance, tensile strength, yield strength, and propagation. This will boost the accuracy and efficiency of spindle. Based on these results, high-precision CNC spindle was developed.

Shear-flow driven ion-acoustic instability in a non-thermal magnetoplasma

Relying on the kinetic model, we study linear properties of ion-acoustic waves in a non-thermal magnetoplasma, whose constituents are the electrons and singly charged positive ions. For this purpose, we solve a set of coupled Vlasov–Poisson equations within the framework of an unperturbed orbit theory and Fourier transformations. A generalized dielectric function is derived in a Cairns distributed plasma to investigate the electrostatic ion-acoustic waves with weak ion shear flows and ion streaming effects. It is found that the magnitude of the growth rate of the wave increases by varying the non-thermality and ion shear flow parameters. Furthermore, the wave spectrum and instability involving the ion-acoustic waves are analyzed with different plasma parameters in a non-thermal plasma. The present findings also reveal both analytical and numerical analyses of the electrostatic waves in the upper ionosphere, where non-thermal plasma particles are common.

PaMYB11 promotes suberin deposition in Norway spruce embryogenic tissue during cryopreservation: A novel resistance mechanism against osmosis.

The osmotic resistance mechanism has been extensively studied in whole plants or plant tissues. However, little is known about it in embryogenic tissue (ET) which is widely used in plant-based biotechnological systems. Suberin, a cell wall aliphatic and aromatic heteropolymer, plays a critical role in plant cells against osmosis stress. The suberin regulatory biosynthesis has rarely been studied in gymnosperms. Here, PaMYB11, a subgroup 11 R2R3-MYB transcription factor, plays a key role in the osmotic resistance of Norway spruce (Picea abies) ETs during cryoprotectant pretreatment. Thus, RNA-seq, histological, and analytical chemical analyses are performed on the stable transformations of PaMYB11-OE and PaMYB11-SRDX in Norway spruce ETs. DAP-seq, Y1H, and LUC are further combined to explore the PaMYB11 targets. Activation of PaMYB11 is necessary and sufficient for suberin lamellae deposition on Norway spruce embryogenic cell walls, which plays a decisive role in ET survival under osmotic stress. Transcriptome analysis shows that PaMYB11 enhances suberin lamellae monomer synthesis by promoting very long-chain fatty acid (VLCFA) synthesis. PaPOP, PaADH1, and PaTET8L, the first two (PaADH1 and PaPOP, included) involved in VLCFA synthesis, are proved to be the direct targets of PaMYB11. Our study identified a novel osmotic response directed by PaMYB11 in Norway spruce ET, which provides a new understanding of the resistance mechanism against osmosis in gymnosperms.