Analysis Of Variance Research Articles

Genetic Variability and Character Association in F3 Generation of Chickpea (Cicer arietinum L.) under Rainfed and Irrigated Conditions

The goal of the current study was to analyze the genetic variability and character association in F3 generations of chickpea under rainfed and irrigated conditions to finalize appropriate selection criteria for improvement of seed yield in chickpea under irrigated and rainfed conditions. Five F3 generations of chickpea crosses were evaluated in compact family block design with three replications at Research Farm, ARSS, Hanumangarh, Rajasthan, India. In analysis of variance, mean sum of squares indicated significant differences among the generations for all the characters under both conditions. The highest range of variation was observed for seed yield per plant followed by fruiting branches per plant, pods per plant, 100 seed weight and seeds per pod in irrigated condition, whereas in rainfed condition the highest range of variation was registered by seed yield per plant followed by biological yield per plant, 100 seed weight and pods per plant. The RSG-895 x RSG-888 showed high GCV and PCV for fruiting branches per plant, pods per plant, biological yield per plant, and seed yield per plant in both conditions, as well as for seeds per pod under irrigated condition. Under irrigated condition high value of GCV and PCV were noted in IPC-94-94 x RSG-888 for harvest and in CSJD-901 x RSG-931 for pods per plant, seeds per pod and seed yield per plant. Under rainfed high GCV and PCV were observed in cross RSG-888 x ICC-4958 for seeds per pod. This indicates the existence of wide variability for these traits in the progenies of particular crosses and scope of improvement through simple selection. High heritability coupled with high genetic advance as percentage of mean (GAM) was noted in RSG-888 x ICC-4958 for pod per plant under both conditions. Under irrigated condition, the cross CSJD-901 x RSG-931 for pods per plant, RSG-895 x RSG-888 for seed yield per plant, RSG-888 x ICC-4958 for harvest index, RSG-888 x ICC-4958 and CSJD-901 x RSG-931 for 100-seed weight showed high heritability along with high GAM. Whereas under rainfed, the crosses RSG-895 x RSG-888 and IPC-94-94 x RSG-888 for pods per plant, BG-362 x RSG-931 for seed yield per plant, and IPC-94-94 x RSG-888 for harvest index, demonstrated high heritability along with high GAM. This result indicates the importance of additive gene action in inheritance of these characters, hence simple selection can be used to improve seed yield. Seed yield per plant exhibited positive and significant correlation with fruiting branches per plant, pods per plant, biological yield per plant, harvest index and 100-seed weight under both irrigated and rainfed conditions. In irrigated condition seed yield per plant had significant and positive correlation with days to 50% flowering, days to maturity and plant height, whereas in rainfed condition it had significant and positive association with protein content. Further, inter se these characters were also significantly positively correlated. So, selection based on these characters is expected to bring improvement in the seed yield.

Multi-response optimisation of wear parameters of B4C reinforced Al-Fe-Si composites: Using Taguchi-grey relational analysis

The current research investigates the effect of boron carbide (B4C) reinforcement on the mechanical and sliding wear behaviour of Al-Fe-Si (AA8011) composites, fabricated using an ultrasonic-assisted stir-casting technique with varying B4C weight fractions from 0% to 10%. Microstructural analysis, including SEM and EDS, confirmed the presence of reinforcement, improved particle dispersion, and grain refinement. The study found that the density of AA8011–B4C composites decreases while porosity increases with higher B4C content. Notably, the AA8011-6 wt.%B4C composite exhibited a significant improvement in mechanical properties, with yield strength, tensile strength, and hardness rising by 91%, 55%, and 38%, respectively, compared to the unreinforced alloy. The optimisation of wear test parameters of AA8011-6 wt.% B4C composites using Taguchi-grey relational analysis and analysis of variance, revealed that the lower wear and coefficient of friction were achieved at a load of 30 N, a disc velocity of 5 m/s, and a sliding distance of 1000 m. The wear test optimisation achieved a 95.67% grey relational grade improvement within the 95% predicted confidence interval.

Comparative study on productivity of Hybrid Napier CO3 and CO4 with the application of organic and inorganic fertilizers

The present study aimed to assess the growth parameters, herbage yield and chemical composition of CO3 and CO4 (Pennisetum perpureum X Pennisetum americarnum) at the Livestock farm Eastern University, Sri Lanka from July to September 2018. Four different treatments; inorganic fertilizer 100%, Organic fertilizer 100%, Control and Inorganic+Organic fertilizer 1:1 (weight basis). Plant height, leaf length, number of tillers per clump, leaf area, yield and total ash content were measured at two-week intervals from the 4th week up to the 8th week and data were subjected to Analysis of Variance (ANOVA). The means were separated using Duncan’s multiple range test at 0.05 significance level. Results indicated that the productivity of CO3 is superior to CO4 in terms of growth and yield under (T4) CO3 with 1:1 mixture of inorganic and organic fertilizer. The highest (p<0.05) fresh matter yield of 18.07 t/ha, with a dry matter yield of 3.83 t/ha was recorded in the sole application of inorganic fertilizer. A similar yield was obtained in CO3 with the 1:1 inorganic and organic fertilizer mixture. CO3 showed the highest total ash content (18.44%) on dry matter basis. Hence, farmers can save half of the cost of inorganic fertilizer by using farmyard manure along with half of the recommended dose of inorganic fertilizer for fodder cultivation

Boosting Career Success: Innovative Behavioral Measures for Guiding Saudi Secondary School Graduates

This study developed and validated a comprehensive behavioral measure for career guidance among secondary school graduates in Saudi Arabia, aiming to integrate soft skills, personality traits, attitudes, and cognitive factors to fill gaps in existing career guidance frameworks. Using a cross-sectional design, data were collected from 814 secondary school students (M_age = 16.47) from diverse urban (n = 407) and rural (n = 407) settings in Saudi Arabia. Multigroup latent profile analyses (LPA) were employed to identify distinct career decision-making profiles among students. The study explored whether urban and rural students exhibited different patterns of engagement in career guidance behaviors. Three distinct engagement profiles were identified: high, moderate, and low engagement, consistent across both settings. High engagement profiles were characterized by high self-efficacy, proactive career exploration, and low anxiety, linked to better career decision outcomes and greater satisfaction with career guidance. Variance analysis using the Games-Howell post hoc test was conducted to compare the profiles based on key behavioral measures such as self-efficacy, career exploration behavior, and anxiety. To further understand the contextual factors influencing these profiles, a multinomial regression analysis examined the impact of teacher support and resilience on career guidance engagement levels. Results indicated that higher levels of teacher support and resilience significantly predicted membership in high engagement profiles. These findings highlight the importance of incorporating behavioral components into career guidance programs, particularly those tailored to address the specific needs of urban and rural students in Saudi Arabia, ultimately supporting the broader educational and workforce development goals of Vision 2030.

Assessing the population structure and genetic diversity of wheat germplasm with the iPBS-retrotransposons marker system

Context Wheat (Triticum aestivum L.) is an important crop that provides food to millions of people all over the world. Currently, wheat production is limited due to various biotic and abiotic stresses resulting from uneven patterns of climate change. Therefore, it is very important to develop climate-resilient wheat cultivars. Crop genetic diversity allows the scientific community to identify genetic variations that can be utilised in the development of improved cultivars. Aims This study planned to characterise the wheat germplasm with the iPBS-retrotransposons marker system. Methods A total of 30 iPBS-retrotransposons markers were screened and among these, the 12 most polymorphic markers were selected for further analysis. Key results Molecular characterisation yielded a total of 170 bands, of which 143 were polymorphic. A substantial level of genetic diversity was observed (mean effective number of alleles: 1.37, Shannon’s information index: 0.23, gene diversity: 0.35). Maximum genetic distance was observed in G9 and G60 genotypes. Analysis of molecular variance revealed that most genetic variation (95%) occurred within the populations. The model-based structure algorithm divided the studied germplasm into three populations based on their collection regions. Similarly, the neighbour-joining analysis also divided 70 tested wheat genotypes into three populations, whereas principal coordinate analysis divided the evaluated germplasm into four populations. Conclusions This study confirms the iPBS-retrotransposons as an ideal marker for the genetic diversity assessment studies for any crop, especially for wheat. Implications The results presented here will be helpful for the scientific community in the marker-assisted breeding of wheat.

Impact of Socio-Demographic Factors on Quality of Life and Coping Strategies of Children with Different Disabilities

Background and Objectives: Children with disabilities face unique challenges that can affect their well-being and quality of life (QOL). This study aimed to assess the QOL and coping strategies adopted by children with disabilities and explore how socio-demographic factors influence QOL and coping strategies. Materials and Methods: This cross-sectional study, which was conducted in Saudi Arabia with children aged 6 to 18 years, used a stratified random sample to ensure representation from a variety of demographic groups. Short Form-12 (SF-12) was used to assess the QOL in the sample population. The Brief COPE Inventory was used to examine coping strategies among the children. One-way analysis of variance was applied to examine differences in the QOL, coping strategies scores, and demographic variables. Multiple regression analyses were performed to examine the role of demographic variables in predicting QOL and p value was considered statistical significance at p < 0.05. Results: The results of the study clearly revealed significant differences between the mean scores of QOL for gender, age, and type of disability, duration of disability, education qualification, family status, family occupation, and housing status. Female participants showed better QOL in physical functioning than their male counterparts. Children with intellectual disability reported better QOL in general health, vitality, social functioning, and mental health. Participants with seven to eight years of disability reported higher scores in physical functioning, vitality, and mental health. Children whose parents were working in private agencies and living in rented houses scored higher on the general health aspects of quality of life. The findings also revealed that the duration of the disability was a significant predictor of the QOL. The mean scores of different dimensions of coping strategies clearly revealed that male participants used dysfunctional coping (p < 0.01), as compared to problem-focused and emotional-focused coping while female children relied more on emotional-focused coping. Emotion-focused coping was significantly higher in participants with visual (p < 0.01), learning (p < 0.01), and intellectual disabilities (p < 0.01). Dysfunctional coping scores were higher among children with auditory disabilities (p < 0.01) and multiple disabilities (p < 0.01). Conclusions: This study highlights the significance of demographic factors in understanding and improving the well-being of a diverse population of disabled juveniles. It offers valuable insights into the subtle factors affecting quality of life. Future interventions and policies can leverage these findings to enhance the quality of life of individuals with disabilities and to foster a more supportive and inclusive approach.

Semaglutide in patients with kidney failure and obesity undergoing dialysis and wishing to be transplanted: A prospective, observational, open-label study.

Chronic kidney disease (CKD) and obesity are major global health challenges, eventually leading to kidney replacement therapy (KRT), but body mass index (BMI) thresholds hinder kidney transplantation. Glucagon-like peptide-1 receptor agonists induce weight loss, thereby offering attractive treatment options; however, their safety and efficacy have not been systematically investigated in patients undergoing dialysis. We conducted a prospective 12-week, open-label trial with 13 patients who had a BMI ≥ 30.00 kg/m2, were undergoing dialysis (12 haemodialysis and 1 peritoneal dialysis) and had not been listed for transplantation due to their weight. Semaglutide was administered once weekly subcutaneously, and the dose was increased from 0.25 mg to 0.5 mg and then to 1 mg. Study endpoints included change in body weight and BMI (primary - statistically evaluated by repeated measures analysis of variance [ANOVA]), side effects, adverse events, blood parameters and patient-reported outcomes (secondary). At baseline, the mean age ± standard deviation of patients was 64.0 ± 6.4 years, the mean weight was 113.9 ± 16.6 kg, and the mean BMI was 37.3 ± 3.9 kg/m2. At week 12, average weight reduction under semaglutide treatment was 4.6 ± 2.4 kg and ranged from 2.0 to 9.7 kg (p < 0.001 for weight and BMI reduction across the study period). One patient discontinued treatment due to nausea/vomiting, two patients died of unrelated causes and six patients reported side effects. Approximately 9 months after the treatment started, three patients were able to seriously reconsider being listed for transplantation. Semaglutide treatment resulted in significant reduction in weight and BMI in patients with obesity undergoing dialysis, while maintaining an acceptable side effect profile comparable to that of the non-dialysis population.

Evaluation of chiropractic students' knowledge and attitudes following pain interventions: A randomized educational trial at 2 institutions.

To examine chiropractic students' attitudes regarding knowledge of pain neuroscience, chronic pain, and patient-centered care before and after educational interventions. Secondarily, this study aimed to compare measures of these skills between cohorts at different timepoints throughout training programs. Using stratified randomization, 281 Year 3 chiropractic students at 2 institutions were allocated into 1 of 3 educational interventions and served as active-control comparison groups: pain neuroscience education, chronic pain education, or patient-centered care. Participants completed validated surveys regarding their experience with the education interventions immediately pre- and post-lecture and 12 weeks after completion. For further comparison, surveys were also completed by 160 Year 1 students and 118 Year 2 students at 1 of the institutions. Independent sample t tests and 1-way analysis of variance were used for data analysis. All Year 3 lecture groups showed immediate improvements (pain neuroscience education: 3.99 + 3.09/100, p = .18 [95% CI: 10.10 to -1.77]; chronic pain education: 0.42 + 0.74/7, p = .02 [95% CI: 0.72 to 0.07]; patient-centered care: 0.25 + 0.12/6, p = .05 [95% CI: 0.12-0.51]), but these were not sustained at the 12-week follow-up (pain neuroscience education: -6.25 + 4.36/100, p = .15 [95% CI: 14.93 to -2.42]; chronic pain education: 0.33 + 0.16/7, p = .19 [95% CI, 0.66 to 0.01]; patient-centered care: 0.13 + 0.13/6, p = .30 [95% CI: 0.41 to -0.13]). Compared to active controls, only the patient-centered care group showed an immediate statistically significant difference. While this study found that immediate improvement in targeted competencies is possible with focused interventions, they were not sustained long term.

Effects of Adler's Theory‐Based nudge volunteer program on middle school students

AbstractAdler emphasized social interest as a social adaptive lifestyle. The study used a nudge strategy to generate social interest in middle school students through a volunteer program. If student gets a good reward for volunteering, it can be a nudge naturally toward social interest. The dependent variables for the volunteer program as a nudge are inferiority complex, social interest, and game over‐immersion. Using convenience sampling, it was assigning 28 participants to two groups each. The experimental group participated in an intervention consisting of 9 sessions of 90 min each, once a week. Both groups were assessed before and after intervention and at a 4‐weeks follow‐up. Program effectiveness was analyzed using repeated measures analysis of variance. The results showed that it was a significant difference in inferiority complex between the experimental and control groups immediately after intervention, and that it was a strong difference in social interest and game over‐emersion both immediately after the experiment and 4 weeks later. Therefore, it proposes that volunteer programs to increase social interest and transition to a pro‐social lifestyle be included as a nudge in school extracurricular activities or curriculum.

Determining Adults' Attitudes towards Cancer Screening, Information Load, and Mythical Causes of Cancer.

To determine the attitudes of adults presenting to primary care services toward cancer screening, information overload, and mythical causes of cancer. Descriptive and correlational type study. The research was conducted face-to-face with 702 adults. The data were collected using a personal information form, the Attitude Scale for Cancer Screening, Cancer Information Overload Scale, and Cancer Awareness Measure Mythical Causes Scale, and evaluated using multivariate analysis of variance. It was determined that adults with chronic diseases, who did not have sufficient information about cancer, who did not have cancer screening, and who thought that cancer was a disease that could not be prevented, had low scores on attitudes towards cancer screening. It was found that adults who were male, literate, had no cancer screening and thought that cancer was an unpreventable disease had a high cancer knowledge burden. Adults who thought that cancer was an unpreventable disease and did not know the causes of cancer had a high rate of mythical causes of cancer. It was determined that adults' attitudes toward cancer screening were negative, information overload was moderate, and awareness was at a low level. Health promotion and promotion strategies should be developed to increase cancer screening and awareness for these groups, public awareness campaigns should be organized, and evidence-based structured interventions should be implemented. These recommended practices can positively influence adults' attitudes towards cancer screening, enable them to manage cancer information effectively, and help them understand the proven causes of cancer.

Utilization of Black Soldier Fly and Housefly as Biodegradation Agents of Dairy Wastes and source of Animal Feed

Accumulation of dairy wastes and shortage of nutritious feed remain the major challenges in dairy cattle production. Such wastes may sustainably be biodegraded by black soldier fly (BSF) larvae and housefly (HF) larvae into their which ca be used as animal feed. This study was done to compare the capacity of using black soldier fly larvae (BSFL) and housefly larvae (HFL) to manage dairy wastes and use of such larvae as livestock feed. Biomass reduction rate, biomass reduction index, bioconversion rate, feed conversion ratio and nutritional profile of resultant BSF and HF prepupae raised on cow dung (CD) and dairy shed effluent (DSE) were determined. In order to ascertain whether the substrates had an effect on BSFL and HFL, analysis of variance (ANOVA) was used, and multiple mean comparisons at P≤0.05 were done using the Tukey HSD post-hoc test. Housefly larvae reared on DSE had the highest biomass reduction rate and biomass reduction index, even though HFL had a shorter development time than BSFL (P≤0.05). Housefly prepupae reared on DSE had the highest crude protein content of 60.1 % of all the HF prepupae counterparts and BSF prepupae reared on DSE and CD (P≤0.05), Hence HF prepupae could be an alternative source of animal feed. Depending on the needs and purpose, utilization of fly larvae to manage dairy waste is viable and effective option

Comparing posterior airway space changes in Class III patients treated with face mask and reverse chin cup

Objectives: The aim was to compare the posterior airway changes in patients treated with a face mask and reverse chin cup and to assess the stability over a 1.5-year follow-up period. Material and Methods: This historical cohort study consisted of two groups containing 20 patients with skeletal Class III malocclusion. The average age of the patients in groups one (Facemask) and two (reverse chin cup) was 7.77 ± 1.30 years and 8.6 ± 1.36 years, respectively. Seventeen cephalometric variables were measured before, after treatment, and after a 1.5-year follow-up. Comparisons were made within each group and between the two groups. Repeated measures, analysis of variance, or Friedman test were used. Results: There was no significant difference between the two groups in the posterior airway space. For patients who used face masks, six variables (AD1-posterior nasal spine [PNS], AD2-PNS, anterior nasal spine [ANS]-PNS, ANS-PNS-PPW, AA-PNS, and PNS-P) considerably increased (P &lt; 0.05). In the reverse chin cup group, three variables (AD2-PNS, ANS-PNS-PPW, and PNS-P) considerably changed (P &lt; 0.05). Moreover, no significant changes (P &gt; 0.05) were observed in any variables after the 1.5-year follow-up period. Conclusion: In this study, the reverse chin cup and face mask effectively corrected Class III malocclusion and significantly increased the posterior airway space. The face mask showed greater enhancement compared to the reverse chin cup, and these improvements were maintained after a 1.5-year follow-up. However, the two groups had no significant difference regarding the posterior airway space changes.

12596 Characterizing Glycemic Variability In Novel Subphenotypes Of Individuals At Elevated Risk For Type 2 Diabetes Using Continuous Glucose Monitoring

Abstract Disclosure: A.J. Kretowski: None. P. Konopka: None. A. Janucik: None. A. Citko: None. A. Paszko: None. M. Klak: None. A. Golonko: None. A. Szklaruk: None. L. Szczerbinski: None. Background: The heterogeneity among individuals at elevated risk for Type 2 Diabetes (T2D) recently has led to the identification of six subphenotypes, each with distinct risks for developing T2D, its complications, and mortality. Our study leveraged Continuous Glucose Monitoring (CGM) to offer a detailed characterization of these subphenotypes, aiming to uncover differences in glycemic variability and control. Methods: CGM data from 616 individuals without diabetes, from the Polish Registry of Diabetes (PolReD) study, classified into six clusters, were analyzed. The PolReD used Freestyle Libre 2 (Abbott) as the CGM device. CGM-derived measures of glucose control and glucose variability were calculated using the iglu R package. We utilized Analysis of Variance (ANOVA) and Analysis of Covariance (ANCOVA), adjusting for age and sex, to identify significant differences in CGM parameters across the clusters. Results: Our analysis identified significant differences in several CGM-derived parameters among the clusters, notably in the Glycemic Risk Assessment Diabetes Equation (GRADE), Standard Deviation of weekly glucose values (SDw), Mean Amplitude of Glycemic Excursions (MAGE), Maximum glucose value, Glucose range, and Mean Absolute Glucose (MAG). Clusters 3 and 5, characterized by beta-cell dysfunction and high insulin resistance respectively, and associated with the highest risk of T2D, exhibited the greatest glucose variability, with elevated risk for glycemic excursions. In contrast, Cluster 4, indicative of a metabolically healthy obesity profile with a low risk of T2D, demonstrated the most stable glycemic control and the lowest variability among the clusters. Conclusions: This study shows that subtypes of patients at elevated risk for T2D exhibit distinct patterns in CGM-derived parameters, indicating variations in glucose control and stability that extend beyond traditional fasting and post-challenge glucose assessments. Notably, we found that subtypes facing the highest T2D risk display increased glycemic variability, despite their differing underlying mechanisms of dysglycemia. These findings highlight the utility of CGM as a tool for identifying individuals at an increased risk of T2D, reducing the need for extensive phenotyping typically required for cluster assignment. Presentation: 6/1/2024

Experimental Study on Fiber Extraction after Saturated Steam Softening of Pleioblastus amarus

At present, bamboo fiber is mainly prepared by rolling and carding after employing the alkali boiling softening method, which is not friendly to the environment. In order to obtain a green and environmentally friendly pretreatment method for preparing bamboo fiber, this paper starts with the current bamboo softening technology and explores the impact of various experimental factors on fiber extraction of Pleioblastus amarus (bitter bamboo) after application of the saturated steam softening method through studying the relationship between saturated steam temperature, holding time, moisture content of bamboo strips, fiber yield, fiber fineness, and the mechanical properties of Pleioblastus amarus fiber. Single-factor experiments revealed that optimal softening fiber extraction effects were achieved within a steam temperature range of 150–180 °C, a holding time of 10–30 min, and a moisture content of 12%–22%. Based on these findings, an orthogonal experiment was designed using a factorial-level table. Through the analysis of range, variance, and orthogonal experiment results, combined with the fibrillation effect and the practical application of fibers, the optimal process parameters of the saturated steam softening method for fiber extraction were determined: saturated steam temperature 170 °C, holding time 20 min, and moisture content 12%. In contrast to the method of conventional mechanical fiber extraction after alkali boiling softening treatment, bamboo fibers processed utilizing the optimized conditions of the saturated steam softening technique showcase a substantial 63% elevation in fiber yield, a notable 18% reduction in fiber fineness, a commendable 28% enhancement in fiber tensile strength, an equivalent tensile modulus, and a marked 53% decrease in elongation at break. The softening process can provide a green and environmentally friendly treatment method for bamboo fiber extraction and greatly promote the scope of application of Pleioblastus amarus.

Study on the Mechanism of Guizhi Fuling Decoction in Treating Hyperlipidemia Based on Network Pharmacology and Experimental Methods

Background Hyperlipidemia, caused by abnormal lipid metabolism, has become a significant health concern, especially in younger populations. While statins are commonly used for treatment, they often cause severe side effects with long-term use, leading to increased interest in finding natural alternatives. Purpose This study aimed to investigate how Guizhi Fuling Decoction (GZFL) treats hyperlipidemia using network pharmacology and experimental validation. Materials and Methods Network pharmacology analyzed GZFL’s active components, targets, and treatment mechanisms for hyperlipidemia. Hyperlipidemia rat and high-fat cell models were established. Experimental validation included enzyme-linked immunosorbent assay, Western blot, quantitative polymerase chain reaction, Oil Red O staining, and other methods. Statistical analysis was performed using one-way analysis of variance followed by Tukey’s post hoc test for multiple comparisons. Pearson correlation analysis was used to identify correlations between key protein expressions and lipid levels. Results GZFL comprises 85 active components and targets 218 proteins. Hyperlipidemia involves 3,852 targets, with 175 overlapping targets. Key targets included estrogen receptor 1, prostaglandin-endoperoxide synthase 2, interleukin-6 (IL-6), protein kinase B (AKT) 1, tumor necrosis factor, interleukin-1 beta, peroxisome proliferator-activated receptor gamma (PPARG), tumor protein p53 (TP53), and fructo oligosaccharide (FOS). Major active components were quercetin, phytosterols, kaempferol, and baicalein. Enrichment analysis highlighted processes like lipopolysaccharide response and lipid signaling pathways. Animal studies showed GZFL significantly reduced serum total cholesterol, triglycerides, and low-density lipoprotein cholesterol levels, and increased apolipoprotein A1 ( p &lt; 0.05). Western blot indicated that GZFL influenced AKT, TP53, IL-6, PPARγ expression, and mRNA levels in the phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) pathway ( p &lt; 0.01). Serum pharmacology experiments demonstrated GZFL-medicated serum reduced lipid droplet formation in high-fat cell models. Conclusion GZFL contains multiple pharmacologically active components, such as quercetin, phytosterols, and kaempferol, which can influence lipid metabolism. It may exert its effects by regulating proteins like AKT, TP53, IL-6, and PPARγ, participating in lipid and atherosclerosis signaling pathways and the PI3K/AKT/mTOR pathway.

6934 Nutritional Factors and Endothelial Dysfunction in Women with Functional Hypothalamic Amenorrhea

Abstract Disclosure: E.R. Uddenberg: None. N. Safwan: None. G. Cook-Wiens: None. O. Obrutu: None. M. Saadedine: None. M.D. Hurtado: None. S. Faubion: None. M.D. Pisarska: None. S.L. Berga: None. J.E. Manson: None. C.N. Bairey Merz: None. C.L. Shufelt: None. Background: Functional hypothalamic amenorrhea (FHA) occurs in women of reproductive age and is a common form of secondary amenorrhea that results in hypoestrogenemia and hypercortisolemia. The underlying cause of FHA is due to a mismatch between energetic intake and expenditure that often manifests as disordered eating, excessive exercise, and psychosocial stress. Previous research identified that 1 in 3 women with FHA had endothelial dysfunction, a preclinical marker for cardiovascular disease. We sought to evaluate the nutrition content in young women with FHA compared to eumenorrheic controls and further assess the relationship to endothelial dysfunction. Methods: We enrolled 30 women with FHA and 29 eumenorrheic controls, not on hormones. FHA was defined as ≥3 consecutive months of amenorrhea, estradiol &amp;lt;50 pg/mL, FSH&amp;lt;10 mIU/L, and LH&amp;lt;10 mIU/L, and exclusion of polycystic ovary syndrome, hyperprolactinemia, thyroid dysfunction, and pregnancy. Eumenorrheic controls had monthly menses with ovulation (progesterone ≥4 ng/mL). Vascular function was measured using EndoPAT 2000 (Itamar® Medical Ltd) to calculate the reactive hyperemic index (RHI). An RHI ≤1.67 indicates endothelial dysfunction. Nutritional intake was collected using a 3-day food diary and analyzed using ESHA Research Food Processor® Nutrition Analysis. Percent (%) recommendation values for nutrition content comparison were calculated based on height, weight, and age. Statistical analysis included Analysis of Variance and Kruskal Wallis test with nonparametric variables reported as median [IQR]. Results: The mean age and BMI of FHA and controls were 26.4 ± 6.2 yrs and 30.3 ± 3.7 yrs (p=0.008), and 21.6 ± 6.2 and 21.8 ± 2.0 kg/m2 (p=0.16), respectively. The median months of FHA amenorrhea was 12 months [5.0, 34.0]. After adjusting for age, there was no difference in caloric intakes in women with FHA compared to controls (1783.9 [1465, 2011] kcal vs. 1731.9 [1578, 1982] kcal, p=0.73); however FHA has higher intake of grams of protein (84.2 [74, 112] g vs. 74 [59, 92] g, p=0.0036), and higher grams of dietary fiber (30.1 [22, 43] g vs. 17.3 [15, 23] g, p=0.0037), respectively. In women with FHA, endothelial dysfunction was associated with a higher % recommended calories (p=0.0098), calories from fat (p=0.02), grams of saturated fat (p=0.03), and grams of carbohydrates (p=0.049). Conclusions: Our results demonstrate that women with FHA consumed similar daily calories, although more grams of protein and dietary fiber than controls. In women with FHA, higher calories, calories from fat, grams of saturated fat and carbohydrates were associated with endothelial dysfunction. Future studies should aim to identify other factors related to endothelial dysfunction among women with FHA and whether some FHA phenotypes are associated with more endothelial dysfunction than others. Presentation: 6/1/2024

6934 Nutritional Factors and Endothelial Dysfunction in Women with Functional Hypothalamic Amenorrhea

Abstract Disclosure: E.R. Uddenberg: None. N. Safwan: None. G. Cook-Wiens: None. O. Obrutu: None. M. Saadedine: None. M.D. Hurtado: None. S. Faubion: None. M.D. Pisarska: None. S.L. Berga: None. J.E. Manson: None. C.N. Bairey Merz: None. C.L. Shufelt: None. Background: Functional hypothalamic amenorrhea (FHA) occurs in women of reproductive age and is a common form of secondary amenorrhea that results in hypoestrogenemia and hypercortisolemia. The underlying cause of FHA is due to a mismatch between energetic intake and expenditure that often manifests as disordered eating, excessive exercise, and psychosocial stress. Previous research identified that 1 in 3 women with FHA had endothelial dysfunction, a preclinical marker for cardiovascular disease. We sought to evaluate the nutrition content in young women with FHA compared to eumenorrheic controls and further assess the relationship to endothelial dysfunction. Methods: We enrolled 30 women with FHA and 29 eumenorrheic controls, not on hormones. FHA was defined as ≥3 consecutive months of amenorrhea, estradiol &amp;lt;50 pg/mL, FSH&amp;lt;10 mIU/L, and LH&amp;lt;10 mIU/L, and exclusion of polycystic ovary syndrome, hyperprolactinemia, thyroid dysfunction, and pregnancy. Eumenorrheic controls had monthly menses with ovulation (progesterone ≥4 ng/mL). Vascular function was measured using EndoPAT 2000 (Itamar® Medical Ltd) to calculate the reactive hyperemic index (RHI). An RHI ≤1.67 indicates endothelial dysfunction. Nutritional intake was collected using a 3-day food diary and analyzed using ESHA Research Food Processor® Nutrition Analysis. Percent (%) recommendation values for nutrition content comparison were calculated based on height, weight, and age. Statistical analysis included Analysis of Variance and Kruskal Wallis test with nonparametric variables reported as median [IQR]. Results: The mean age and BMI of FHA and controls were 26.4 ± 6.2 yrs and 30.3 ± 3.7 yrs (p=0.008), and 21.6 ± 6.2 and 21.8 ± 2.0 kg/m2 (p=0.16), respectively. The median months of FHA amenorrhea was 12 months [5.0, 34.0]. After adjusting for age, there was no difference in caloric intakes in women with FHA compared to controls (1783.9 [1465, 2011] kcal vs. 1731.9 [1578, 1982] kcal, p=0.73); however FHA has higher intake of grams of protein (84.2 [74, 112] g vs. 74 [59, 92] g, p=0.0036), and higher grams of dietary fiber (30.1 [22, 43] g vs. 17.3 [15, 23] g, p=0.0037), respectively. In women with FHA, endothelial dysfunction was associated with a higher % recommended calories (p=0.0098), calories from fat (p=0.02), grams of saturated fat (p=0.03), and grams of carbohydrates (p=0.049). Conclusions: Our results demonstrate that women with FHA consumed similar daily calories, although more grams of protein and dietary fiber than controls. In women with FHA, higher calories, calories from fat, grams of saturated fat and carbohydrates were associated with endothelial dysfunction. Future studies should aim to identify other factors related to endothelial dysfunction among women with FHA and whether some FHA phenotypes are associated with more endothelial dysfunction than others. Presentation: 6/1/2024

Optimizing EFL learning: exploring the role of learner background factors and the nuances of their effects on intrinsic and extrinsic motivation among university students in a mixed-methods study

IntroductionMotivation is a driving force behind man’s behavior which has led to many psychological studies throughout the world. Moreover, it is the fuel for successful learning. While intrinsic motives provide the internal rewards, extrinsic motivation supplies the required external rewards to keep the engine of learning running. Knowing the factors which impact intrinsic/extrinsic motivation helps educators target their efforts at a higher level and make more informed decisions.MethodThis study intends to examine how intrinsic vs. extrinsic motivation may be influenced by demographics including learners’ background factors of major, age, and occupation and to determine the relationships that may exist between intrinsic and extrinsic motivation. To this end, an explanatory sequential mixed-methods research was conducted at one of the public universities in Shiraz on the majors of Elementary Education, Educational Affairs, Social Studies and Theology, and the Arabic language which were selected through cluster sampling. These students were having their English courses at the university. Based on Krejcie and Morgan’s formula for sample size, a questionnaire was administered to 100 participants to collect quantitative data. Moreover, semi-structured interview sessions were conducted with one fourth of the participants.ResultsUsing Multiple Analysis of Variance (MANOVA) and Pearson correlation, we found that learners’ background factors of age, field of study and professional status did not affect intrinsic nor extrinsic motivation. However, significant and positive relationships were found between intrinsic motivation and total motivation index, and between extrinsic motivation and total motivation index. Qualitative data obtained from the interviews were analyzed using a thematic analysis. The results of the interviews showed some new illuminating trends as revealed from the participants’ responses and it was found that most interviewees followed intrinsic motives and considered motivation as a factor of great significance.ConclusionIn the English as a Foreign Language (EFL) context in which our study was conducted, instructors can follow more similar classroom motivational techniques and strategies since neither type of motivation was influenced by the variables of different major, age and occupation. Though many studies have shown that EFL learners are more extrinsically motivated in comparison with ESL ones, our study revealed that participants were more intrinsically motivated. Thus, this study may be replicated in other educational contexts such as an ESL context. The study can also be repeated in some universities in which other educational systems such as coed education is used to see the possible similarities and differences. Motivation is the important stimulant to impel the learners to achieve their learning goals; thus, it should receive sufficient attention in various educational settings.

Screening and Site Adaptability Evaluation of Qi-Nan Clones (Aquilaria sinensis) in Southern China

In recent years, plantations of Aquilaria sinensis in China have been dominated by Qi-nan, yet there remains limited research on the growth evaluation and breeding of these clones. In this study, a multi-point joint variance analysis, an additive main effect and multiplicative interaction (AMMI) model, a weighted average of absolute scores (WAASB) stability index, and a genotype main effect plus a genotype-by-environment interaction (GGE) biplot were used to comprehensively analyze the yield, stability, and suitable environment of 25 3-year-old Qi-Nan clones from five sites in southern China. The results showed that all the growth traits exhibited significant differences in the clones, test sites, and interactions between the clones and test sites. The phenotypic variation coefficient (PCV) and genetic variation coefficient (GCV) of the clones’ growth traits at the different sites ranged from 16.56% to 32.09% and 5.24% to 27.06%, respectively, showing moderate variation. The medium–high repeatability (R) of tree height and ground diameter ranged from 0.50 to 0.96 and 0.69 to 0.98, respectively. Among the clones, Clones G04, G05, G10, G11 and G13 showed good growth performance and could be good candidates for breeding. Environmental effects were found to be the primary source of variation, with temperature and light primarily affecting growth, while rainfall influenced survival and preservation rates. Yangjiang (YJ) was found to be the most suitable experimental site for screening high-yield and stable clones across the different sites, whereas the tree height and ground diameter at the Chengmai (CM) site were significantly higher than at the other sites, and the Pingxiang (PX) and Zhangzhou (ZZ) sites showed poor growth performance. The findings suggest that Qi-nan clones are suitable for planting in southern China. There were also abundant genetic variations in germplasm resources for the Qi-nan clones. The five selected clones could be suitable for extensive planting. Therefore, large-scale testing is necessary for determining genetic improvements in Qi-nan clones, which will be conducive to the precise localization of their promotion areas.

Three-Dimensional Reconstruction of Partially Coherent Scatterers Using Iterative Sub-Network Generation Method

Synthetic aperture radar tomography (TomoSAR) has gained significant attention for three-dimensional (3D) imaging in urban environments. A notable limitation of traditional TomoSAR approaches is their primary focus on persistent scatterers (PSs), disregarding targets with temporal decorrelated characteristics. Temporal variations in coherence, especially in urban areas due to the dense population of buildings and artificial structures, can lead to a reduction in detectable PSs and suboptimal 3D reconstruction performance. The concept of partially coherent scatterers (PCSs) has been proven effective by capturing the partial temporal coherence of targets across the entire time baseline. In this study, an novel approach based on an iterative sub-network generation method is introduced to leverage PCSs for enhanced 3D reconstruction in dynamic environments. We propose a coherence constraint iterative variance analysis approach to determine the optimal temporal baseline range that accurately reflects the interferometric coherence of PCSs. Utilizing the selected PCSs, a 3D imaging technique that incorporates the iterative generation of sub-networks into the SAR tomography process is developed. By employing the PS reference network as a foundation, we accurately invert PCSs through the iterative generation of local star-shaped networks, ensuring a comprehensive coverage of PCSs in study areas. The effectiveness of this method for the height estimation of PCSs is validated using the TerraSAR-X dataset. Compared with traditional PS-based TomoSAR, the proposed approach demonstrates that PCS-based elevation results complement those from PSs, significantly improving 3D reconstruction in evolving urban settings.