Analysis Of Seed Proteins Research Articles

Two-dimensional Gel Analysis of Total Seed Protein of Horse Gram (Macrotyloma uniflorum) for Identification of their Proteomic Diversity

Horse gram (Macrotyloma uniflorum) is a popular pulse in the Kumaun region of Uttarakhand, locally known as Gahat dal and Kulti. An experiment was conducted with 5 horse gram cultivars having different grain colors. Total seed protein was isolated and quantified with standard protocols. Five protein samples were subjected to one-dimensional (1D) and two-dimensional (2D) gel for proteomic analysis. 300g of each sample was loaded onto immobilized pH gradient (IPG) strips (3–10 pH Linear, 18 cm) and kept for Iso-Electric Focusing (IEF) and SDS-PAGE. The gels were scanned using a scanner and analyzed for proteomic diversity. Basically, two major clusters were constructed, and samples 1, 2, and 3 are in group one and 4 and 5 are in group two, and group one has two further sub-clusters. Sample one was separately clustered based on the number of spots presented on the 2D gel and based on its color. The analysis of variance revealed that the genotypes observed had highly significant differences. Further study is needed for the identification of unique peptides presented by protein sequencing. The proteomic analysis of total seed proteins could be useful for the development of nutraceuticals and food-to-food fortification. This study’s current proteome and other proteomic information will be extremely useful to horse gram breeding programmes.

Protein patterns and their association with photosynthetic pigment content, agronomic behavior, and origin of purslane accessions (Portulaca oleracea L.).

In this study, the proteomic, morphometric, and photosynthetic pigment data of purslane (Portulaca oleracea) accessions were combined together to show their impact on genetic variation in order to establish a relationship between protein patterns and phenotypic behavior of the plant. Seeds of 18 collected purslane accessions were cultivated based on a completely randomized design with three replicates. Before the flowering stage, the data on morphology, photosynthetic pigment content, and seed proteins were obtained. The results showed a significant difference among purslane accessions in terms of the most studied agronomic characteristics and the content of photosynthetic pigments and proteins. The cluster analysis of the 18 purslane accessions based on agronomic data, and photosynthetic pigment content, and protein pattern data produced three main clusters. Moreover, the seed protein analysis revealed that the two polymorphic protein bands of size 40 kDa (protein "a") and 30 kDa (protein "b") effectively diversified the agronomic, photosynthetic pigment, and phylogenetic relationships among the purslane accessions. Interestingly, protein "a" was produced in plants growing in low altitude areas and played a suppressive role for TDW, while protein "b" was produced in plants growing in high altitude areas and functioned as an activator agent for this trait. Overall, the outcomes of the present study indicated the presence of high genetic variability (77.6%) among the purslane accessions. These findings suggest that these proteins should be sequenced for further proteomic analyses and can be used for hybridization to generate useful recombinants in segregating generations and improve breeding varieties of P. oleracea.

Varietal identification of Thai hot chilli using seed protein analysis by ultrathin layer isoelectric focusing technique

Varietal identification of Thai hot chilli using seed protein analysis by ultrathin layer isoelectric focusing technique

SDS-PAGE based protein profiling and diversity assessment of indigenous genotypes of cucumber

The present investigation was carried out to determine the relationships among genotypes categorization of seed storage proteins profiles of 44 genotypes with two checks PantKhira-1 and Pointsette of cucumber (Cucumis sativus L.) was performed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) during from July-October, 2014 and February-June, 2015. The entire profile comprised of 15 protein bands, distributed into three major zones A, B and C in increasing order of electrophoretic mobility i.e., Zone A was nearest and Zone C was farthest from the point of protein sample application. Among 15 bands maximum number of bands were observed in PCUC-193 (9 bands), PCPGR-6762 (8bands), PCPGR-7013 (8bands) and PCPGR-7795 (8bands) followed by PCUC-199 (7 bands), PCUC-832(7 bands), PCUC-44 (7 bands), PCUC-23 (6 bands). Minimum bands were shown by PCPGR-7795 (1 band). The un weighed pair group method using arithmetic average (UPGMA) analysis of 46 cucumber genotypes was done and two major clusters obtained through seed protein analysis expressed grouping of genotypes of cucumber. Approximately 83.339 and 83.332 per cent of variation was recorded due to first five principle components on various genotypes in first season and second season respectively. The first four principle components recorded a per cent variation of 66.617 among various genotypes in pooled analysis. Protein profiling of genotypes resolved all forty six indigenous germplasm lines into different groups on the basis of presence and absence of fifteen protein bands distributed into A, B and C zone respectively.

Seed Protein Profiling of Tomato (Solanum lycopersicum L.) Genotypes using SDS-PAGE

A quantitative categorization of seed storage proteins profiles of 13 genotypes of Solanum lycopersicum L. was performed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). The banding patterns were characterized by 3 clear distinct zones viz., A, B and C. The unweighed pair group method using arithmetic average (UPGMA) analysis of 13 tomato genotypes was done and two major clusters obtained through seed protein analysis expressed better grouping of genotypes. The dendrogram showed that the genotype EC-519724 was most dissimilar from other genotypes.

A Proteomic Analysis of Seed Proteins Expressed in a Brassica Somatic Hybrid and its two Parental Species

Cabbage (Brassica oleracea) is one of the world’s major vegetable crops. However, the cabbage plant cannot be readily vernalized and produce seed in the tropics, whereas Chinese cabbage (Brassica campestris) can be conveniently vernalized at the seed stage. Proteins from seeds of an IPSA cabbage-Chinese cabbage somatic hybrid showing both hybrid vigour and the seed-stage vernalization characteristic were analyzed and compared with those of its two parents. 1D gel analysis indicated some differential expression of the seed proteins. Furthermore, in 2D, gel analysis, 13 differentially expressed protein spots were examined: of these ten were found to belong to the cruciferin seed storage protein complex, two were unidentifiable (no MASCOT match), and one was identified as late embryogenesis Abundant (LEA)-like protein, and was present only in the cabbage parent. This LEA-like protein in cabbage seed could therefore be implicated in interfering with the vernalization process in the tropics. Plant Tissue Cult. & Biotech. 24(1): 11-26, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19187

Hybridization in the Sida ovata complex (Malvaceae) III. Evidences from seed micro-morphology and seed protein analysis

In this paper, we present a detailed analysis of seed morphological characters and seed protein composition of two closely related species belonging to the Sida ovata complex (Malvaceae), Sida ovata Forssk. and S. tiagii Bhandari. Seed protein profiling was performed by sodium dodesyl sulphate–polyacrylamide gel electrophoresis and size exclusion‐fast protein liquid chromatography. Our data provide additional information to strengthen the existence of hybridization between Sida ovata Forssk. and S. tiagii Bhandari in Pakistan.

Assessment of genetic relatedness of the two Amaranthus retroflexus populations by protein and random amplified polymorphic DNA (RAPD) markers

Two populations of Amaranthus retroflexus with different morphology were collected from field of the Maize Research Institute Zemun Polje, Serbia. Random amplified polymorphic DNA (RAPD) and seed protein analysis were performed to study the genetic differences in two grain Amaranthus populations. The studied populations have different protein and DNA profile. A total of 171 DNA fragments were generated by 31 RAPD primers, with an average of 5.5 fragments per primer. Of these, 61.4% fragments were polymorphic among the two populations. 18 protein fraction were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The populations differed in the four protein fractions of different molecular weight. The seed protein electrophoresis and RAPD markers are useful for genetic determination of A. retroflexus populations and identification of biotypes with atypical morphology.

Electrophoretic Analysis of Seed Proteins from Different Varieties of Rice Cultivated in Sindh

SDS-PAGE electrophoresis is the most effective as well as one of the simplest methods for separating proteins. The extraction and fractionation technique was applied to characterize rice seed proteins. Identification and screening of rice proteins carried out from 14 varieties of rice collected from Nuclear Institute of Agriculture (NIA) Tandojam. Rice seed proteins were extracted with phosphate buffer for total proteins. After quantification, proteins were separated by SDS-PAGE Electrophoresis. Analysis of rice seed proteins was performed on the results of SDS-PAGE using gel documentation system (Biorad) to find out the molecular weight of different varieties of rice followed by cluster analysis using SPSS to find out the diversity among different rice varieties.

Morphological and seed protein characterization of the cultivated and the wild taxa of Sesamum L. (Pedaliaceae)

Seed protein analysis and morphological characterization were carried out in one cultivated and one wild species of Sesamum, Sesamum indicum L. and S. occidentale Regel and Heer. Data on 13 quantitative and 33 qualitative characters of the cultivated species and seven accessions of the wild taxa were analyzed. The genetic diversity of the taxa was assessed using UPGMA dendrogram and one-way ANOVA (p < 0.05). Principal component analysis (PCA) was executed to identify the significant characters to delimit the taxa. Seed protein analysis showed diverse bands, ranging from 16 to 88 kDa. A dendrogram based on UPGMA analysis of seed protein suggested intraspecific relationships of the wild taxa as evidenced from the morphological characterization.

Phenotypic and seed protein analysis in 31 Lima bean (&lt;i&gt;Phaseolus lunatus&lt;/i&gt;) accessions in Ghana

Phenotypic and seed protein analyses were performed on 31 accessions of Lima bean assembled in Ghana. Data on 16 phenotypic characters consisting of eight quantitative and eight qualitative were analysed. There were significant differences among the accessions based on the eight quantitative characters. Seed protein analysis showed 17 bands with relative mobility of bands, which ranged from 0.01 to 0.86. An ordinal logistic regression analysis showed significant evidence for seed coat, pod beak shape and seed size association. Cluster analysis based on both phenotypic and protein data provided evidence for differences among the accessions. Quantitative characters were associated with some specific clusters.

Genetic diversity, seed size associations and population structure of a core collection of common beans (Phaseolus vulgaris L.)

Cultivated common bean germplasm is especially diverse due to the parallel domestication of two genepools in the Mesoamerican and Andean centers of diversity and introgression between these gene pools. Classification into morphological races has helped to provide a framework for utilization of this cultivated germplasm. Meanwhile, core collections along with molecular markers are useful tools for organizing and analyzing representative sets of these genotypes. In this study, we evaluated 604 accessions from the CIAT core germplasm collection representing wide genetic variability from both primary and secondary centers of diversity with a newly developed, fluorescent microsatellite marker set of 36 genomic and gene-based SSRs to determine molecular diversity and with seed protein analysis to determine phaseolin alleles. The entire collection could be divided into two genepools and five predominant races with the division between the Mesoamerica race and the Durango-Jalisco group showing strong support within the Mesoamerican genepool and the Nueva Granada and Peru races showing less diversity overall and some between-group admixture within the Andean genepool. The Chile race could not be distinguished within the Andean genepool but there was support for the Guatemala race within the Mesoamerican genepool and this race was unique in its high level of diversity and distance from other Mesoamerican races. Based on this population structure, significant associations were found between SSR loci and seed size characteristics, some on the same linkage group as the phaseolin locus, which previously had been associated with seed size, or in other regions of the genome. In conclusion, this study has shown that common bean has very significant population structure that can help guide the construction of genetic crosses that maximize diversity as well as serving as a basis for additional association studies.

Effects of Flurenol on Soybean (Glycine max L. Merrill) Productivity and Electrophoretic Analysis of Seed and Root Nodule Proteins

Effects of Flurenol on Soybean (Glycine max L. Merrill) Productivity and Electrophoretic Analysis of Seed and Root Nodule Proteins

Taxonomic relationships in genusFritillaria(Liliaceae): Evidence from RAPD-PCR and SDS-PAGE of seed proteins

The genus Fritillaria L. is one of the examples from the several unsolved taxonomic problems. The classical identification of plant depends on morphological characteristics. Therefore, it is difficult to determine the botanical origin of some plants. The goals of this study were to examine the taxonomic status of these 42 taxa of Fritillaria in Turkey by means of RAPD-PCR and seed protein analysis in addition to taxonomic interpretation of species. SDS-PAGE and RAPD-PCR techniques were used to help taxonomic interpretation of Fritillaria species. Dendrogram based on g enetic distances was calculated using the computer programmed PopGen. For the RAPD-PCR analysis, 40 random primers were tested in the amplification reactions with Fritillaria species. Only 9 of them gave consistently reproducible banding patterns. The analysis of seed proteins showed that all studied genotypes had a specific protein pattern except the species that were close to each other based on morphological data. In electrophoretic prote...

Proteomic Analysis of Lupin Seed Proteins To Identify Conglutin β as an Allergen, Lup an 1

Lupin products may be valuable as human foods because of their high protein content and potential anticholesterolemic properties. However, a small percentage of the population is allergic to lupin. In this study, we use in vitro IgE binding and mass spectrometry to identify conglutin beta, a major storage protein, as an allergen in seeds of Lupinus angustifolius and Lupinus albus. Purification of conglutin beta from L. angustifolius flour confirmed that serum IgE binds to this protein. Where IgE in sera recognized lupin proteins on Western blots, it recognized conglutin beta, suggesting this protein is a major allergen for lupin. The L. angustifolius conglutin beta allergen has been designated Lup an 1 by the International Union of Immunological Societies (IUIS) allergen nomenclature subcommittee.

Differentiation ofVitis viniferavarieties by MALDI‐MS analysis of the grape seed proteins

Until now the study of pathogenic related proteins in grape juice and wine, performed by ESI-MS, LC/ESI-MS, and MALDI/MS, has been proposed for differentiation of varieties. In fact, chitinases and thaumatin-like proteins persist through the vinification process and cause hazes and sediments in bottled wines. An additional instrument, potentially suitable for the grape varieties differentiation, has been developed by MALDI/MS for the grape seed protein analysis. The hydrosoluble protein profiles of seeds extract from three different Vitis vinifera grape (red and white) varieties were analyzed and compared. In order to evaluate the environmental conditions and harvest effects, the seed protein profiles of one grape variety from different locations and harvests were studied.

Seed protein analyses as a support to the transfer of Trigonella cylindracea Desv. and T. polyceratia (L.) Trautv. to genus Medicago L.

T he present study has been planned to investigate the extent to which seed proteins and peptide mapping may be used as evidences to discuss the position of T. cylindracea Desv. and T. Polyceratia (L.) Trautv. To achieve this objective two outgroups (reference species): Trigonella foenum-graecum L. and Medicago sativa L., represent the type species for each genus were used. Each species was considered as an operational taxonomic unit (OTU) for the purpose of classification and ordination. The bands from 1D-SDS-PAGE or the spots from 2D-peptide mapping were scored as 0 representing absence and 1 representing presence, and employed in the analysis by using NTSYS-pc computer and PAST programs. Cluster analysis and PCA using the protein data from the two different techniques demonstrated that the similarity between T. polyceratia and M. sativa is more than that to T. foenum-graecum . The present paper accepts the transfer of T. polyceratia to Medicago and recommends further studies for T. cylindracea .

Jointed Goatgrass (Aegilops cylindrica Host) × Wheat (Triticum aestivum L.) Hybrids

ABSTRACTThe ability of the weedy species jointed goatgrass (Aegilops cylindrica Host) to form seed‐bearing hybrids with wheat (Triticum aestivum L.) raises questions concerning the potential movement of herbicide‐resistance genes from commercial wheat cultivars into the weed population. As a preliminary step for future gene‐flow risk assessments, a study of jointed goatgrass × wheat hybrids collected from infested wheat fields in 1998 and 1999 was undertaken in Oregon. Jointed goatgrass accessions representing the range of variation in its worldwide distribution also were included in this study. The high molecular weight (HMW) glutenins, a group of wheat endosperm storage proteins, were used as genetic markers for characterizing this material. In the Oregon jointed goatgrass accessions, the seed protein analysis identified F1 hybrid seed that was formed at a rate of 0 to 8% on a per field basis. The HMW glutenin patterns in the backcross seed threshed from Oregon hybrids showed a higher proportion of seeds formed from pollination by wheat than by jointed goatgrass. Analysis of the roots for remains of the maternal seed or spikelet indicated that most hybrid plants were of the F1 generation and that either jointed goatgrass or wheat could be the female parent. These analyses suggested a hybridization dynamics in which jointed goatgrass serves as the predominant F1 female parent and wheat as the predominant backcross male parent. Development of introgressed jointed goatgrass forms carrying wheat genes would be dependent on the presence of a continuous hybrid zone located near or within a persistent jointed goatgrass population.

Analysis of Seed Protein and Oil from Soybean Northern and Southern Region Uniform Tests

Protein and oil concentration are seed constituents that make soybean [Glycine max (L.) Merr.] an important crop. Reports have been published that the oil concentration and recently the protein concentration of U.S. soybean has been decreasing with respect to other exporting countries. The objective of this study was to utilize 51 yr of two data sets, The Uniform Soybean Tests, Northern Region and Southern Region to determine long-term trends in these constituents. Oil and protein composition data of lines from Regional Summaries of Maturity Groups OO through VIII were used for the analysis. Mean oil concentration was higher in the Southern Region compared with the Northern Region (209 and 206 g kg -1 , respectively), as was protein concentration (411 and 407 g kg -1 , respectively). Maturity group means for oil and protein concentration ranged from 198 to 212 g kg -1 and 404 to 414 g kg -1 , respectively. Oil concentration increased in both data sets to 1973 and abruptly decreased in 1974 (21 g kg -1 ) to a low in 1982 (184 g kg -1 ) to higher than average values in 1983 (217 g kg -1 ) and by linear regression analysis, decreasing in the Northern Region since then. Analysis of lines evaluated from 1973 to 1974 and from 1982 to 1983 indicated that genetics was not the cause of the change in oil concentrations. Temporal protein concentrations were not as distinct as those of oil but indicated groups of years when protein concentration was above or below the mean. The data indicate that oil concentrations of soybean has been erratic and decreasing since 1974 because of unknown factors.

TOMATO ECOTYPE CHARACTERIZATION BY ANIONIC EXCHANGE¿HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF ENDOSPERM SEED PROTEINS

ISHS International Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture TOMATO ECOTYPE CHARACTERIZATION BY ANIONIC EXCHANGE¿HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF ENDOSPERM SEED PROTEINS