SDS-PAGE based protein profiling and diversity assessment of indigenous genotypes of cucumber

Abstract

The present investigation was carried out to determine the relationships among genotypes categorization of seed storage proteins profiles of 44 genotypes with two checks PantKhira-1 and Pointsette of cucumber (Cucumis sativus L.) was performed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) during from July-October, 2014 and February-June, 2015. The entire profile comprised of 15 protein bands, distributed into three major zones A, B and C in increasing order of electrophoretic mobility i.e., Zone A was nearest and Zone C was farthest from the point of protein sample application. Among 15 bands maximum number of bands were observed in PCUC-193 (9 bands), PCPGR-6762 (8bands), PCPGR-7013 (8bands) and PCPGR-7795 (8bands) followed by PCUC-199 (7 bands), PCUC-832(7 bands), PCUC-44 (7 bands), PCUC-23 (6 bands). Minimum bands were shown by PCPGR-7795 (1 band). The un weighed pair group method using arithmetic average (UPGMA) analysis of 46 cucumber genotypes was done and two major clusters obtained through seed protein analysis expressed grouping of genotypes of cucumber. Approximately 83.339 and 83.332 per cent of variation was recorded due to first five principle components on various genotypes in first season and second season respectively. The first four principle components recorded a per cent variation of 66.617 among various genotypes in pooled analysis. Protein profiling of genotypes resolved all forty six indigenous germplasm lines into different groups on the basis of presence and absence of fifteen protein bands distributed into A, B and C zone respectively.