The amount and annual distribution of rainfall caused a major socioeconomic and environmental problem where rainfed agriculture is predominant. This study assessed the long-term variability and trends of rainfall in the Wabi Shebele River Basin (WSRB), Ethiopia. The basin was discretized into 9 local agroclimatic zones (ACZ) based on annual rainfall and elevation. The coefficient of variation (CV) was used to check the variability of rainfall while modified Mann-Kendall (MK) and Innovative Trend Analysis (ITA) methods were used to detect rainfall trends. For each ACZ, stations with long-term records and less than 10% of missing data were selected for further analysis. The mean annual rainfall in the basin ranges from 227.2 mm to 1047.4 mm. The study revealed most of the ACZs showed a very high variation in Belg/Spring rainfall (CV% > 30) than Kiremt/Summer and annual rainfall. Seasonal and annual rainfall trend analysis revealed that no uniform trend was detected in all ACZs. However, most of ACZs in the arid and semi-arid areas showed a non-significant decreasing trend in annual rainfall. From seasonal analysis, Belg and Kiremt rainfall showed relatively decreasing and increasing trends respectively. In comparison, a similar result was observed using MK and ITA methods.