Analysis Of Purity Research Articles

Residual Solvent Signal of CDCl3 as a qNMR Internal Standard for Application in Organic Chemistry Laboratory

A nuclear magnetic resonance (NMR) spectrometer is a key instrument in the organic synthesis laboratory for structure determination, reaction control, and compound purity analysis. In addition to qualitative analysis, the application of NMR for quantitative analysis (qNMR) is gaining popularity. qNMR allows for simple quantification of crude product mixtures, determination of reaction yields, and purity of organic compounds. The determination of NMR yield requires the addition of an internal standard to each sample. Herein, we report a method where CDCl3 residual solvent signal is used as an internal standard for qNMR after quantification in the solvent batch. This method significantly simplifies sample preparation and allows straightforward recovery of the analyte by the simple evaporation of the NMR solvent. The accuracy of the method is comparable to qNMR with 1,3,5-trimethoxybenzene as an internal standard if the herein described guidelines are followed.

Facile mild hydrothermal treatment for surface functionalization of carbonized sawdust

This work illustrated the preparation of highly dispersive carbonized sawdust by surface functionalization through a facile mild hydrothermal treatment with an aqueous mixture of reactants, potassium persulfate and potassium hydroxide (KPS:KOH), at 160 °C. The dispersion, morphological and purity analyses play an important role in the determination of the optimal hydrothermal process parameters. Dispersion test unveiled well dispersed modified carbonized sawdust's in polar solvent (ethanol) and no significant morphological changes according to the field emission scanning electron microscopy (FESEM) images. The intensity ratio (ID/IG) from the Raman measurement displayed an increased in the modified carbonized sawdust's ID/IG which indicated successful surface functionalization. Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) spectra revealed the introduction of various functional groups onto the carbonized sawdust surface, while the increment in the oxygen and potassium elements percentage (at. %) based on the energy dispersive X-ray system (EDX) supported both FTIR and XPS findings. Carbonized sawdust treated in 1:3 M ratio of KPS:KOH mixture (0.07 M:0.21 M) for 60 min showed the best dispersion in ethanol for at least a month with high degree of functionalization and various oxygen based functional groups were attached on its surface.

Rheological behavior of stabilized diamond-graphene nanoplatelets hybrid nanosuspensions in mineral oil

Recent advancements in thermo-fluid technology assisted with highly thermal conductive nanomaterials have shown assuring outcomes. It is also proven that thermal conductivity alone cannot define the overall heat transfer characteristics, and the viscous properties are equally significant towards thermal management. Therefore, this research involves investigating the rheological behavior of hybrid nanosuspensions containing high thermally conductive diamond and graphene nanoplatelets (1:1). These nanomaterials are dispersed in mineral oil using a two-step technique. Hybrid nanofluids' stability is achieved using a non-ionic stabilizer Span85, exhibiting no sedimentation for a minimum of five months. Nanomaterial characterizations are performed to study morphology, purity, and chemical analysis. The flow behavior of hybrid nanosuspensions is investigated at varying nanomaterial mass concentrations (0–2%), temperatures (298.15–338.15 K), and shear rates (1–2000 s−1). Hybrid nanofluids exhibit shear-thinning behavior, which is also correlated with the Ostwald-de-Waele model. The temperature-viscosity relationship is well predicted using the Vogel-Fulcher-Tammann model. Hybrid nanofluids show a maximum enhancement of 35% viscosity at 2% concentration. A generalized two-variable correlation is used to express viscosity as a function of temperature and nanofluid concentration with an excellent agreement. Three different machine learning methods, i.e., Artificial Neural Network (ANN), Gradient Boosting Machine (GBM), and Random Forest (RF) algorithms are also introduced to predict the viscosity of hybrid nanofluids based on the three input parameters (temperature, concentration, and shear rate). The parity plots conclude that all algorithms can predict big-data viscous behavior with high precision.

Capillary Electrophoresis-Sodium Dodecyl Sulfate with Laser-Induced Fluorescence Detection as a Highly Sensitive and Quality Control-Friendly Method for Monitoring Adeno-Associated Virus Capsid Protein Purity.

The capsid protein purity of adeno-associated virus (AAV) is considered a critical quality attribute of AAV-based gene therapy products. However, the analytical methods currently available to monitor the viral capsid proteins, which are present in extremely low concentrations, have limited sensitivity and robustness, thus limiting their general applicability. As a result, there is an urgent need to develop robust separation methods with highly sensitive detection. In this article, we describe the first denaturation and fluorescence labeling procedure for AAV capsid proteins using the pyrylium dye Chromeo™ P503, enabling the establishment of the first capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) method combined with laser-induced fluorescence (LIF) detection for AAV. Upon optimization using a quality-by-design approach, the newly developed method features a simple and robust one-step sample preparation workflow resulting in consistently labeled and denatured viral protein samples, which can subsequently be separated and quantified by CE-LIF. The method has been validated to be accurate and precise with a linear range of 50-150% of the nominal concentration of 2.0 × 1011 vector genomes per mL (vg/mL). The detection limit and quantitation limit were established to be 8.0 × 107 vg/mL (∼0.8 ng/mL) and 4.2 × 108 vg/mL (∼4 ng/mL), respectively, representing the highest sensitivity achieved for AAV capsid protein quantitation reported to date and a linear dynamic range of 8.0 × 107-3.0 × 1011 vg/mL. A comparison of the CE-SDS LIF method with existing methods, such as CE-SDS ultraviolet and sodium dodecyl sulfate-polyacrylamide gel electrophoresis with SYPRO Ruby stain, indicated that the new method has superior resolution and a significant increase in signal intensity. Capsid protein purity analysis of multiple AAV serotypes, including AAV5, scAAVrh10, AAV2, and AAV6, has been demonstrated for the first time using the same method, indicating the newly developed AAV labeling procedure and CE-LIF analysis could serve as a Quality Control-friendly platform and best-in-class analytical method for the control of AAV capsid protein purity.

Polarization studies on Rayleigh scattering of hard x rays by closed-shell atoms

We present a theoretical study on the elastic Rayleigh scattering of x-ray photons by closed-shell atoms. Special attention is paid to the transfer of linear polarization from the incident to the outgoing photons. To study this process, we apply the density-matrix formalism combined with the relativistic perturbation theory. This formalism enables us to find general relations between the Stokes parameters of the incident and scattered photons. By using these expressions, we revisit the recent proposal to use Rayleigh scattering for the analysis of the polarization purity of synchrotron radiation. We show that this analysis can be performed without any need for the theoretically calculated scattering amplitudes, if the linear polarization of the scattered light is measured simultaneously at the azimuthal angles ${0}^{\ensuremath{\circ}}$ and ${45}^{\ensuremath{\circ}}$ with respect to the plane of the synchrotron. To illustrate our approach, we present detailed calculations for scattering of 145 keV photons by lead atoms.

Assessment of Genetic Purity in Rice Using Polymorphic SSR Markers and Its Economic Analysis with Grow-Out-Test

Genetic purity is conventionally performed through grow-out-test (GOT) with morphological characters. Simple sequence repeat (SSR) markers are independent of G X E interaction. Herewith, 16 high yielding varieties of rice were analysed using 55 SSR markers for DNA fingerprinting and identification of genetic impurities: 14 were found to be polymorphic and amplified 48 alleles with an average of 3.43 alleles per each primer pair. The number of alleles amplified ranged from 2 to 6 and the size of the PCR products amplified from these 14 primer pairs ranged from 80 to 450 bp with polymorphic information content (PIC) from 0.14 (RM 346) to 0.99 (RM 5900). PICs at 0.5 or higher are highly informative SSR markers for genetic studies and the study reported (7/14) markers to be highly informative based on PIC values. Besides, the values of effective multiplex ratio, marker index and resolving power for the selected polymorphic primer pairs disclosed that the SSR markers used in the study are highly informative and could be potentially used for distinctness, uniformity and stability (DUS) testing, genetic purity analysis and DNA fingerprinting of rice varieties. Economic analysis of grow-out-test and SSR marker based genetic purity showed that the GOT process incurs Rs. 5.8 (INR) per seed (USD 0.07), while DNA-based marker analysis incurs Rs. 53.75 (INR) or $ 0.71 (USD).

Uporaba DNA markerjev za genetsko identifikacijo navadnega (Avena sativa L.) in golega ovsa (Avena nuda L.) pri preverjanju sortne pristnosti

The usefulness of genetic identification of varieties for seed quality analyses becomes important, when we suspect the presence of another variety, species or even genus, based on morphological seed traits in the purity analysis and germination test, With genetic analyses, the doubt about the authenticity of the naked oats (Avena nuda L.) variety 'Kamil' was successfully solved. There were atypical seeds with chaff among the samples of this variety, so it was not possible to confirm with certainty, whether these were seeds of the same species/variety or impurities of common oats (Avena sativa L.). Based on the seed phenotype of the 'Kamil' variety (two independent samples 284 and 285), four sub-samples were prepared (284AN, 284AS, 285AN, 285AS); AN label was the sub-sample with naked seeds and AS label was the sub-sample with chaffed seeds. In addition, another variety ('Noni') and four accessions of common oats (ACC378, ACC379, ACC380, ACC381) from the Slovene Plant Gene Bank were included as common oat standards. A total of 36 plant samples of Avena sp. were included in the genetic differentiation. Using a set of six highly informative SSR markers and the results of diversity parameters at individual loci and paired genetic comparisons, we were able to confirm that the analysed sub-samples belonged to the same variety, i.e. 'Kamil'. In addition, despite the morphological similarity, the chaffed seeds of naked oats variety ‘Kamil’ were sufficiently (genetically) different from the analysed variety and/or accessions of common oats.

Chapter 3: The purity analysis; Chapter 4: Other seeds by number

Chapter 3: The purity analysis; Chapter 4: Other seeds by number

Optimization of Silica Extraction from Diatomaceous Earth using the Central Composite Design of Response Surface Methodology

ABSTRACT This work evaluated the extraction efficiency of silica from diatomaceous earth (DE) by conventional solvent extraction (CSE) and ultrasound-assisted extraction (UAE) under basic and acidic (HCl) conditions utilizing response surface methodology (RSM). A common statistical model was used to get the best percentage yield and percentage purity using RSM. Experimental parameters such as stirring time, NaOH concentration (for CSE), sonication time, NaOH/HCl concentration, cycle and amplitude (for UAE methods) were optimized using central composite design (CCD). The analytical responses, that is, percentage yield and percentage purity, were analyzed using ANOVA and regression analysis. The linear coefficient of determination, R2, was high and precise. The overall yield and purity were highest for the UAE method under acid condition (pH 1-2, approximately), making it the most effective silica extraction method from DE. For this method, the optimal conditions for extracting Si from DE were 2 h of sonication, 220 mL of 2.82 M HCl as a leaching/extracting medium, 0.524 cycles and 72.6 % of amplitude. Under these conditions, 82 % of silica was yielded with a purity of about 95 %. The experimental results indicate that high-quality silica can be produced from DE in high yield so that DE can be an alternative silica source. Keywords: Diatomaceous earth, silica, response surface methodology, ultrasound-assisted extraction, conventional solvent extraction.

Development of a downstream process for purification and purity analysis of glutaminase free L-asparaginase using UPLC, DLS-ZP and DSC-TGA

Several bacterial strains were isolated from soil and water in the current work and screened for glutaminase-free L-asparaginase activity. The selected strain was subjected to the production of glutaminase-free L-asparaginase and thereby purification. Before purification, pH scouting was performed, and 8.6 pH was the optimum working pH to carry out purification. The enzyme was purified using Sephadex G-200 gel filtration chromatography and up to 4.55-folds and its purity percentage was determined using UPLC. The purity of L-asparaginase was determined to be 99.2%. The enzyme was characterized using UV-Visible Spectrophotometer, DLS, ZP, DSC, TGA. The loss of the enzyme was 65% after sublimation, indicating its purity compared to the standard drug. The results report a developed process for the purification of L-asparaginase and its characterization, which can prove instrumental in the purification process of naturally occurring enzymes because today, most developed processes deal with recombinant enzymes.

Optimal Energy Consumption of the Distillation Process and Its Product Purity Analysis Using Ultraviolet Spectroscopy.

This paper addresses the energy consumption of distillation process via an actuator, which is a challenging problem in process industries. Precise control action would enhance energy consumption and improve the productivity. This paper is an experimental validation of EPC-PI control algorithm and analysis of distillate purity of a lab-scale distillation column. The PI control scheme uses closed-loop data of extended predictive controller (EPC) that has been performed through off-line simulation. The performance of control method is compared with different schemes such as Hägglund’s one-third rule and Skogestad’s overshoot method. The issue of integral windup in the multivariable process is addressed in the aspect of optimal energy consumption. The energy consumption calculations are made with respect to power utility of actuators throughout the process. The distillate product of post-controller implementation is processed to qualitative analysis using UV spectroscopy. Performance index is carried out via integral time absolute error (ITAE) by perturbing plant parameters up to 30% uncertainty.

Development of a machine vision system for rice seed inspection system

Rice seed production in Malaysia is greatly dependent on the purity of the cultivated paddy seed produced through the government certified paddy seed program. The seeds to be marketed by the seed processors must undergo quality control protocol where the seed lots are sampled from the seed farms and seed processing plants for purity analysis by the enforcing agency at the Seed Testing Laboratory of the Department of Agriculture (DoA). The current inspection conducted by the laboratory is based on a manual process, which is laborious and time-consuming. Therefore, a prototype (Patent ID: PI2018500018) of a machine vision-based rice seed inspection system (RiSe-IViS) was developed to explore the possibility of replacing the existing manual method in distinguishing the weedy rice and cultivated rice seeds under the Standard Jabatan Pertanian Malaysia (SJPM) standard protocol with a modern, effective and efficient technique using an image processing approach. The developed RiSe-IViS prototype consists of two parts i) hardware configuration and ii) software development. This paper discussed the criteria to be established, challenges and limitation encountered in developing the hardware prototype involving the image acquisition setup, lighting configuration and seed plate design. The importance of each criterion to ensure its reproducibility are also discussed. A software programme was developed to assist the user for image acquisition and analysis. The image processing steps undertaken in the programme are also discussed. The RiSe-IViS is expected to classify major rice seed varieties available in Malaysia against the weedy rice variants with superior accuracy.

Application of SSR Marker in Genetic Purity Analysis of CORH 4 Rice Hybrid and its Parental Lines

Grow out test is used for assessing the genetic purity but molecular markers like simple sequence repeats can be used as alternative. The present study aims at evaluating the efficacy of SSR marker in genetic purity assessment. SSR marker RM570 which differentiate the CMS 23A and CB 174R lines with the allelic size of 260 and 300 bp respectively. Four hundred paddy seeds comprising 2 off type and 398 original seeds of A, R and CORH 4 were sown in 20rows×20columns matrix in a sand medium and leaf samples from seedlings were bulked row and column wise. RM570 produced single allele for genetically pure bulking and two alleles for bulking with off types. Further, the identified off-types were investigated by individual leaf analysis using RM570 marker. Hence, SSR markers can be employed to the assessment of genetic purity rather than conventional grow out test.

Evaluation of the Efficiency of Protein A Affinity Chromatography to Purify a Monoclonal Antibody for Cancer Treatment and its Purity Analysis

Background: Protein A affinity chromatography is often employed as the most crucial purification step for monoclonal antibodies to achieve high yield with purity and throughput requirements. Introduction: Protein A, also known as Staphylococcal protein A (SPA) is found in the cell wall of the bacteria staphylococcus aureus. It is one of the first discovered immunoglobulin binding molecules and has been extensively studied since the past few decades. The efficiency of Protein A affinity chromatography to purify a recombinant monoclonal antibody in a cell culture sample has been evaluated, which removes 99.0% of feed stream impurities. Materials and Method: We have systematically evaluated the purification performance by using a battery of analytical methods SDS-PAGE (non-reduced and reduced sample), Cation Exchange Chromatography (CEX), Size-exclusion chromatography (SEC), and Reversed phased-Reduced Chromatography for a CHO-derived monoclonal antibody. Results and Discussion: The analytical test was conducted to determine the impurity parameter, Host Cell Contaminating Proteins (HCP). It was evaluated to be 0.015ng/ml after the purification step; while initially, it was found to be 24.431ng/ml. Conclusion: The tests showed a distinct decrease in the level of different impurities after the chromatography step. It can be concluded that Protein A chromatography is an efficient step in the purification of monoclonal antibodies.

Physicochemical stability of the bevacizumab biosimilar, ABP 215, after preparation and storage in intravenous bags

Study Objectives: To evaluate extended in-use stability of bevacizumab biosimilar, ABP 215, after dilution into intravenous bags, extended storage, and simulated infusion to enable advanced preparation and storage. Methods: Two lots of ABP 215 were diluted to high- (16.5 mg/mL) and low- (1.4 mg/mL) dose concentrations in two types of intravenous bag under ambient light conditions. Dosed intravenous bags were stored at 2°C–8°C for 35 days, followed by 30°C for 2 days, and each bag was infused on Day 37. Analysis of purity and physicochemical stability was performed using size-exclusion high-performance liquid chromatography (SE-HPLC), cation-exchange high-performance liquid chromatography (CEX-HPLC), reduced capillary electrophoresis-sodium dodecyl sulphate (rCE-SDS), subvisible particle detection assays, visual inspection, and by measuring protein concentration and potency. Results: No meaningful changes were seen in ABP 215 purity when analysed by SE-HPLC, CEX-HPLC and rCE-SDS following dilution, storage and infusion of two lots, bags, and doses. Protein concentration remained consistent throughout the study for all samples and no significant loss in potency was detected. No potentially proteinaceous particles or increases in subvisible particles were observed. Discussion: This study investigated the in-use stability of ABP 215 following dilution, extended storage, and infusion, that represent worst-case handling conditions. ABP 215 exhibited consistent product quality and activity, with no significant degradation observed under the conditions tested. Conclusion: ABP 215 retains physicochemical stability after dilution over the recommended dosing concentrations, extended storage, and simulated infusion. This supports the advance preparation and storage of ABP 215 in intravenous bags for infusion.

Analysis of the individual variability of hemp (Cannabis sativa L.) cultivars using SSR and SCAR markers

Relevance . The revival of hemp farming, which is observed in many countries, places high demands on the efficiency of breeding and genetic work and new species of hemp. The genetic diversity of hemp species for economically valuable traits is quite limited, and legal requirements for the content of cannabinoids in the plant complicate the selection process. The use of various DNA markers can provide significant support in effective and safe breeding, analysis of genetic polymorphism and intra-variety purity. The purpose of this work was to study the genetic diversity of a number of modern Russian species of hemp seeds using two types of DNA markers. Materials and methods . The object of the study was eight species of monoecious hemp of the Central Russian type included in the State register of agricultural achievements approved for use, of various breeding origin. Each specie was represented by eight individual plants. To study genetic polymorphism, a PCR method was used using a set of ten SSR markers, followed by product detection on a genetic analyzer. To determine the cannabinoid chemotype of species, a specific SCAR marker was used, the amplification products of which were detected by electrophoresis.

Liquid chromatography at critical conditions (LCCC): Capabilities and limitations for polymer analysis

This paper investigates liquid chromatography at critical condition (LCCC) for polymer analysis. Based on controversial claims on the separation of cyclic polymers from linear analogues in the literature, the efficiency of LCCC for separation and purity analysis is questioned. Polyisobutylene (PIB) and poly(3,6-dioxa-1,8-octanedithiols) (polyDODT) were used for the study. The structure of low molecular weight cyclic and linear polyDODT was demonstrated by MALDI-ToF. NMR did not show the presence of thiol end groups in higher molecular weight PIB-disulfide and polyDODT samples, so they were considered cyclic polymers. When a low molecular weight polyDODT oligomer with only traces of cycles, as demonstrated by MALDI-ToF, was mixed with an Mn = 27 K g/mol cyclic sample, LCCC did not detect the presence of linear oligomers at 6 wt%. Based on the data presented here, it can be concluded that the LCCC method is not capable of measuring <6 wt% linear contamination so earlier claims for cyclic polystyrene (PS) samples purified by LCCC having <3% linear contaminants are questioned.

Application of SSR Markers for Purity Testing of Hybrid Wheat (Triticum aestivum L.)

Exploitation of the full potential of any hybrid requires the possessing of genetically high-purity seeds. In order to avoid reduction in yield caused by using low purity seeds, development of a simple, rapid, and accurate method for hybrid purity assessment is of great essence and significance. For the identification of true hybrids, SSR markers provides authentic information that will help to the further progress of any research programme. In this study, total of 40 wheat hybrids and 14 parental lines were taken for the hybrid purity analysis with 20 SSR markers. These markers were used to find out the codominant loci in the hybrid and single dominant loci in parents. Out of 20 SSR markers, only 8 markers viz., Xwmc617, Xwmc457, Xwmc48, Xgwm153, Xbarc61, Xgwm273, Xbarc268 and Xgwm274 showed the polymorphic dominant loci in the parents and co-dominant loci midway between these parents. Therefore these SSR markers were used to confirm the forty hybrids. The range of allele size produced by SSR marker on the parental lines were 120 bp to 300 bp. The highest allel size (300 bp) was produced by marker Xbarc 268 whereas, the minimum allel size (120 bp) was produced by marker Xgwm 273. All the fourty hybrids showed similar banding pattern as parental lines, this proved the purity of hybrids in all these cross combinations. The confirmation of hybrid purity indicated that a single polymorphic marker was sufficient for detection of contaminations of these hybrids from their parents.

Quantitation of ultra-trace nitrated polycyclic aromatic hydrocarbons isomers in water by online solid-phase extraction coupled-liquid chromatography-mass spectrometry

An online solid-phase extraction (SPE)-coupled liquid chromatography-mass spectrometry (LC-MS) method was established for the determination of 10 nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) in water. Water samples were mixed with methanol to generate 40% methanol solutions (v/v), and filtered by 0.45 μm membrane. The filtration with polytetrafluoroethylene(PTFE) membrane got higher recovery rates than nylon membrane, especially for 4-ring and 5-ring nitro-PAHs. 2.5 mL solution was directly injected into online SPE flow path to allow for online purification and enrichment of target analytes in the SPE column. The nitro-PAHs eluted from the SPE column were automatically transferred to the analytical flow path by a well-designed valve-switching system. With the optimization of LC and MS condition, ten nitro-PAH isomers was separated and detected from each other by LC-MS/MS with negative atmospheric pressure chemical ionization (APCI). It was firstly found that nitro-PAHs could produce strong [M-H]− precursor ions in the primary MS besides [M+e]− and [M+15]−. In the secondary MS, the precursor ions mainly lose NO neutral molecule (30 Daltons) to produce daughter ions. The online SPE and LC-MS analysis process was completed in 15.5 min. The linear correlation coefficients of 10 nitro-PAH standard curves were higher than 0.99. The detection limits of nitro-PAHs were about 1.2~22.2 ng/L (S/N=3). The intra-day and inter-day reproducibility (RSD, n=6) were 1.6%~8.4% and 5.3%~16.9%, respectively. The recoveries of 10, 40 and 200 ng/L in tap water were 71.7%~106.4%, 79.7%~100.9% and 73.0%~105.5%, with the corresponding RSD of 2.4%~10.5%, 2.1%~8.6% and 2.7%~6.2%, respectively.

Structural and magnetic investigation of Al3+ and Cr3+substituted Ni–Co–Cu nanoferrites for potential applications

Nanocrystalline Al3+ and Cr3+ doped Ni–Co–Cu ferrites with the chemical composition Ni0.7Co0.2Cu0.1Al0.5xCr0.5xFe2-xO4 (x = 0.0, 0.1, 0.2) were prepared for demonstrating their structural, surface morphological and magnetic characteristics. The technique used for synthesizing the doped and undoped Ni–Co–Cu nanoferrites was sol-gel auto-combustion technique. The existence of single phase was confirmed with the help of X-ray diffractometry (XRD) which indicates the presence of spinel cubic structure for the synthesized specimens. The crystallite size (D) was determined from the highest intense (311) peak of the observed x-ray diffraction patterns by Debye-Scherrer formula and it was found in the dimensions of 44–55 nm. FESEM micrographs shows the existence of agglomerated and spherical magnetic nanoparticles with an average grain size of 63–78 nm. The prepared samples were examined with energy dispersive x-ray spectroscopy for the analysis of purity of synthesized samples and thus, from the EDX spectrum, only peaks of nickel, cobalt, copper, aluminium, chromium, iron and oxygen were observed which confirms the high purity of our results to a next level. The magnetic study was taken into consideration by vibrating sample magnetometer (VSM) and therefore, from the M − H curves data, various magnetic parameters were calculated. From the M − H loops measurements, the maximum of saturation magnetization (Ms) observed was 52.49 emu/g along with few hundreds of coercivity (Hc) indicating the ferromagnetic and soft behaviour of synthesized specimens. Therefore, in the present investigation, excellent values of saturation magnetization (28.11–52.49 emu/g) and coercivity (536.6–591.02 Oe) were obtained making these nanoferrites suitable for the high-density recording media and electromagnets application.