Analysis Of Phytochemical Constituents Research Articles

Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS

Abstract. Syarpin, Permatasari S, Pujianto DA. 2023. Analysis of phytochemical constituents and antioxidant activity from the fractions of Luvunga sarmentosa root extract using LCMS/MS. Biodiversitas 24: 733-740. Luvunga sarmentosa, one of the native plants of Central Kalimantan, has been used by Dayak people, especially its root, to increase sexual activity, cure sexual dysfunction and increase male fertility. Dayak people boil the stems and roots of L. sarmentosa and drink the extract. However, studies on L. sarmentosa are still limited to phytochemical content and bioactivity screening of the root extract of L. sarmentosa. This research aimed to determine the phytochemical content and antiradical activities of L. sarmentosa root fractions. Fractions were obtained using solvents with different polarities. The sample was extracted by maceration using ethanol, and the extract was fractionated by column chromatography using hexane, chloroform, ethyl acetate, and methanol. The chemical content of each fraction was identified using LCMS/MS. The antiradical activity was performed against DPPH using a UV-Visible spectrophotometer. The LCMS/MS analysis results indicated that each fraction contained different percentages of compounds, such as alkaloids, terpenoids, phenylpropanoids, stilbenes, flavonoids, tannins, fatty acids, and steroids. Ostruthin was a compound detected in all fractions with a retention time of 20,917 minutes and was considered a marker compound from the roots of L. sarmentosa. The antiradical activity assay showed that the methanol fraction had the best activity with an IC50 value of 83.42 ppm. The IC50 data shows that increasing the percentage of alkaloid compounds increased their antioxidant activity.

Analysis of Phytochemical Constituents and Antioxidant Potential of Bitter Kola Leaf Extract towards Bioactive Food, Nutrition and Health Resources

Organic and Medicinal chemistry international Journal is an open access journal that is committed to publish the papers on various topics of chemistry, especially synthetic organic chemistry, and pharmacology and various other biological specialties

Phytochemical and Pharmacognostical evaluation of an anti-inflammatory and Hapatoprotective poly-pharmaceutical preparation “Qurs-e-Zarishk”

Since the time Hippocrates man has made the use the different Unani medicines for the treatment of various ailments and still vast majority of population depends on these drugs. So the herbal drugs are gaining more and more attention all over the world due to their long historical clinical practice and less side effects. “Qurs- e- Zarishk” is one of the most important poly-pharmaceutical Unani formulation widely used for the treatment of liver disorders and anemia. Since the formulation has a very effective role in curing such diseases with zero side effects (unlike in Modern Medicine) so all the ingredients used in this preparation are required to be authenticated and standardized properly for quality control purposes. The present paper deals with different physicochemical and biological parameters like botanical identification, microbial load, aflatoxin, pesticide residue, determination of heavy metals, qualitative and quantitative analysis of phyto-chemical constituents, solubility in alcohol and water, successive extractions, pH values, ash values, quantitative estimation of total phenolics, resins etc. Besides, TLC studies of successive extracts have also been carried out.

Analysis of Phytochemical Constituents by Using LC-MS, Antifungal and Allelopathic Activities of Leaves Extracts of Aloe vera

Analysis of Phytochemical Constituents by Using LC-MS, Antifungal and Allelopathic Activities of Leaves Extracts of Aloe vera

Analysis of phytochemical constituents and antibacterial activity of Wrightia tinctoria: traditional medicinal plant of India for application on wound dressing materials

Wrightia tinctoria is one of the important traditional medicinal plants used in the treatment of various diseases. The idea of the research is to study the presence of phytochemicals and test the antibacterial activity of Wrightia tinctoria leaf extracts on nonwoven fabrics to find its suitability for wound dressings. The methodology includes identification and collection of W.tinctoria leaves, preparation of leaf powder, determination of physicochemical analysis, extraction using different solvents, preliminary phytochemical screening, quantitative estimation of phytoconstituents, yield of the extracts and determination of antibacterial activity on treated fabrics. Results revealed the presence of more active metabolites in the extracts of W.tinctoria which may be the reason for the promising antibacterial potential against the bacterial strains. As a promising ethnomedicinal plant, Wrightia tinctoria may serve as a major source of useful drugs finding its suitability for developing wound dressings.

Comparative analysis of Phytochemical Constituents and Antioxidant activity of two Pomegranate (Punica granatum L.) cultivars

Abstract Pomegranate (Punica granatum L.) is a widely used fruit, which contains several interesting bioactive compounds and possess health-promoting properties. Seeing the importance of this fruit, it is crucial to study its quality characteristics. Hence, in the current study, we assessed phenolic content, flavonoid content and antioxidant activity of different plant parts (leaves, rind and arils) of two pomegranate cultivars (Kesar and Ganesh). The analysis of phytochemical constituents in the cultivars revealed that, the content of phenolics ranged from 49.3 to 602 mg TAE/g plant material and the content of flavonoids ranged from 0.27 to 18.8 mg RE/g plant material. The maximum amount of phenolics and flavonoids was observed in the rind extracts as compared to the leaves and arils in both the cultivars. On the other hand, the antioxidant activity determined by different antioxidant models exhibited various degrees of antioxidant activity in different plant parts of the cultivars and was found to be highest in rind and aril extracts than that of leaves. Among the solvents methanol and ethanol gave highest extraction yields as compared to water and acetone. Total phenolics and flavonoids were significantly different with methanol and ethanol being the most efficient solvent and acetone being the least efficient one. Correlation between the antioxidant activity and phenolics and flavonoid content was found to be significant. Thus the results strongly indicated that there were significant differences between pomegranate cultivars. Generally the cultivar Ganesh has higher antioxidant activity as well as total phenolics and flavonoid content in comparison to Kesar.

Analysis of phytochemical constituents, antibacterial, antioxidant, photoprotective activities and cytotoxic effect of leaves extracts and fractions of Aloe vera

Abstract The aim of the study was to investigate the Aloe vera phytochemical compounds using HPLC–mass spectrometry and to evaluate their antibacterial, antioxidant, photoprotective and cytotoxic activities. The acetone extract, methanol extract and its four fractions: chloroform, ethyl acetate, n- butanol, aqueous were subjected to evaluate their antibacterial activity by Aromatogram method against S.aureus, B. cereus, E. coli, P. aeruginosa, A. baumanii. The minimum inhibitory concentrations (MIC) were determinated by the medium agar incorporation method. Then, the antioxidant properties of all extracts and fractions were evaluated by using Hydroxyl radical scavenging, Galvinoxyl radical scavenging and Phenanthroline assays. The photoprotective activity was measured by determination in vitro of sun protection factor. The cytotoxicity of extracts and fractions was tested by Brine shrimp lethality method. The yields of methanol, chloroform, ethyl acetate, n-butanol, aqueous, acetone extracts are respectively 20.56% ± 0.38, 3.4% ± 0.11, 1.58% ± 0.12, 14.16% ± 0.11, 13.56% ± 0.78, 0.68% ± 0.50. By LC-MS analysis, 8 bioactive phytochemical compounds were identified. The antibacterial activity showed that the n-butanol fraction, acetone and methanol extracts had an effect against all the bacteria. The MIC value ranged from 1.25 to 10 mg/mL. The chloroform fraction exhibited highest antioxidant activity in (OH•2) scavenging assay (IC50 value: 79.53 ± 0.66 μg/mL) and in GOR scavenging assay (IC50 value: 80.18 ± 1.21 μg/mL), the acetone extract exhibited highest antioxidant activity in phenanthroline assay (A0.50 value: 46.75 ± 0.35 μg/mL). Furthermore, the all extracts and fractions showed high photoprotective activity. The acetone, chloroform and ethyl acetate fractions have displayed significant effect against the brine shrimp larvae.

ANTIOXIDANT, PTP 1B INHIBITION AND Α-AMYLASE INHIBITION PROPERTY AND GC-MS ANALYSIS OF METHANOLIC LEAVES EXTRACT OF ACHYRANTHES ASPERA AND CATHARANTHUS ROSEUS OF NEPAL

Objective: The present study was designed to study phytochemicals and biological activities of the methanolic extracts of two traditional medicine plants Achyranthes aspera and Catharanthus roseus of Nepalese origin.
 Methods: Plant extracts were prepared by cold percolation method. Antioxidant activity, brine shrimp lethality assay, and analysis of phytochemical constituents were carried out using standard methods. The dinitro salicylic acid (DNS) method was used to study the inhibition effect of extracts on α-amylase enzyme. Furthermore, PTP 1B inhibitory activity was evaluated using p-nitrophenyl phosphate (p-NPP) as substrate.
 Results: Phytochemical analysis showed the presence of phytochemicals like alkaloids, flavonoids, glycosides, reducing sugars, etc. in both plants. Brine shrimp lethality assay suggested the presence of pharmacologically active compounds. Total phenolic content and total flavonoid content of C. roseus were found to be higher with 73.21 mg GAE/g and 33.15 mg Q/g respectively than that of A. aspera, which was found to be 57.09 mg GAE/g and 28.96 mg Q/g respectively. Similarly, the α-amylase inhibition of A. aspera and C. roseus was found to be 97.60±1.11 µg/ml and 94.05±1.18 µg/ml comparative with IC50 68.13±0.46 µg/ml of standard acarbose. Protein tyrosine phosphatase 1B (PTP1B) inhibition showed IC50 for A. aspera and C. roseus to be 48.72±0.46 and 50.21±1.03 µg/ml, respectively. Qualitative GC-MS analysis of both plant hexane fractions showed acid and ester type of phytoconstituents.
 Conclusion: These results suggested that both plants i. e A. aspera and C. roseus, Nepal origin showed biological activity by targeting multiple drug targets which justifies their traditional uses.

Evaluation of phytochemical constituents and hepatoprotective effects of Physcia grisea in albino rats

Objectives: The study aimed to evaluate the phytochemical constituents and hepatoprotective effects of Physcia grisea in albino rats. Methods/statisticalanalysis:The analysis of phytochemical constituents of P. grisea was carried out to determine the level of tannins, phenols, terpenes, flavonoids and alkaloids present in the sample. The blood samples from four groups (A, B, C, and D) of albino rats with each group containing four per group were analyzed for Aspartate aminotransferase (AST) and bilirubin secretions which are two early indicators of liver injury. The analysis was carried out in two phases; phase 1 and phase 2. In phase I, which was regarded as the base line analysis, the weights of the animals were taken and the blood samples were collected following ocular puncture. In the second phase of the analysis, the group A, B and C were administered 500 mg/kg, 250 mg/kg and 125 mg/kg concentrations of P. grisea respectively while the control group was administered only tragecant solution through the oral route. After 3 weeks of the administration, their blood samples were collected for another analysis using the same method of blood collection. The data obtained were analyzed using GenStat Release 10.3DE (2011) statistical software. Findings: The results showed varying levels of phytochemicals in P. grisea, alkaloids (36.6%), flavonoids (3.6 %), saponins (2.7 %), total phenols (25.15 %), tannins (0.029 %) and terpenes (2.0 %). The results of the in-vivo studies showed that P. grisea had no significant effect on the AST and bilirubin secretions at 125mg/kg after 3 weeks of the administration. This was an indication that there was no liver injury at this dose within the period of administration. Application/improvements: If properly harnessed, P. grisea extract could provide a novel and cheap source of medicine for treatment of liver diseases resulting from AST and bilirubin secretions. Keywords: Phytochemical; Physea grisea; Aspertate Aminotransferase; Bilirubin

Phytochemical screening and antioxidant profiling of Sumatran wild mangoes ( Mangifera spp.): a potential source for medicine antidegenerative effects.

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16mg/g dry weight with the lowest value in M. foetida2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of drugs that have antidegenerative effects.

Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida 3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16 mg/g dry weight with the lowest value in M. foetida 2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

Phytochemical screening and antioxidant profiling of Sumatran wild mangoes ( Mangifera spp.): a potential source for medicine antidegenerative effects

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida 3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.23-35.48 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.16 mg/g dry weight with the lowest value in M. foetida 2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of drugs that have antidegenerative effects.

Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes ( Mangifera spp.): a potential source for antidegenerative medicine.

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.2270-35.4763 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.47 mg/g dry weight with the lowest value in M. foetida2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

Phytochemical analysis of baby corn silk extracts

BackgroundBaby corn silk extract can be used as an antioxidant dietary supplement. However, insufficient data exists for this extract to guarantee its quality, efficacy and safety. ObjectiveTo determine phytochemical constituents, contents of phenolics and flavonoids, antioxidant activities, heavy metal concentrations, and microbial contamination of baby corn silk extracts. Material and methodsBaby corn silks including Pacific 271 and Zeba SG 17 hybrids were individually extracted with 40% v/v ethanol and distilled water to obtain Pacific 271 ethanol extract (PE), Zeba SG 17 ethanol extract (ZE), Pacific 271 aqueous extract (PA), and Zeba SG 17 aqueous extract (ZA). The analysis of phytochemical constituents was carried out using phytochemical tests, TLC screening, UV-visible, FTIR, and 1H NMR experiments. The contents of phenolics and flavonoids were determined using the modified Folin-Ciocalteu and aluminium chloride colorimetric procedures, respectively. Antioxidant activities were investigated using DPPH and FRAP assays. The concentrations of heavy metals were analyzed by ICP-MS. Microbial enumeration tests were carried out according to the United States Pharmacopeia (USP) 41. ResultsPE and ZE were composed of flavonoids, tannins, terpenoids, and steroids while PA and ZA contained flavonoids and tannins. PE and ZE exhibited higher total phenolic and flavonoid contents and significantly stronger antioxidant activities than PA and ZA. All extracts conformed to the microbiological and heavy metal requirements according to Association of South East Asian Nations (ASEAN) guidelines. ConclusionPE and ZE were considered appropriate to use as natural extracts of phenolics and flavonoids with antioxidant activities and safety.

SCIENTIFIC VALIDATION OF SOME NEPALESE MEDICINAL PLANTS FROM NANGLE BHARE OF KATHMANDU, NEPAL

Objective: The present study was designed to provide scientific support for the medicinal properties of traditionally used medicinal plants of Nangle Bhare, Kathmandu, Nepal.
 Methods: Plant extracts were prepared by cold percolation method using methanol solvent. Analysis of phytochemical constituents was carried out using standard methods. The brine shrimp toxicity assay for each extract was carried out according to Mayer et al. The 1, 1-diphenyl-2-picrylhyrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay were used to evaluate in vitro antioxidants activities. The antimicrobial activity of the plant extracts was carried by the disc diffusion method.
 Results: Phytochemical analysis revealed the availability of secondary metabolites such as reducing compounds, glycosides, and flavonoids in the methanolic extract of eight plants, namely, frond of Cheilanthes farinosa, leaves of Lyonia ovalifolia, leaves and roots of Rumex nepalensis, aerial stem of Equisetum debile, roots of Coix lacrema-jobi, leaves of Justica adhatoda, leaves of Osyris wightiana, and roots of Rubus ellipticus collected from Nangle Bhare village of Kathmandu district. The brine shrimp bioassay revealed that roots and leaves of R. nepalensis only exhibited lethality against brine shrimp. Frond of C. farinosa and roots of R. nepalensis exhibited good antimicrobial activity against Staphylococcus aureus and Escherichia coli. Both DPPH and FRAP assays showed that among the plant under study, R. ellipticus has lower inhibitory concentration IC50 values than other plants such as L. ovalifolia, R. nepalensis, and C. farinose. 
 Conclusions: On the basis of this study, it was found that scientifically only four, namely C. farinosa, L. ovalifolia, R. nepalensis, and R. ellipticus have medicinal potentials.

ANALYSIS OF PHYTO-CONSTITUENTS, ANTIOXIDANT, AND ALPHA AMYLASE INHIBITORY ACTIVITIES OF PERSEA AMERICANA MILL., RHODODENDRON ARBORETUM SM. RUBUS ELLIPTICUS SM. FROM ARGHAKHANCHI DISTRICT NEPAL

Objective: To evaluate the phytochemical, antioxidant activities, and α-amylase inhibition assay for methanolic extract of three ethnomedicinal plants, namely Persea americana Mill., Rubus ellipticus Sm., and Rhododendron arboretum Sm. collected from Arghakhanchi District of Nepal using in vitro studies.Methods: Methanolic plant extracts were prepared by cold percolation method. Analysis of phytochemical constituents was carried out using standard methods. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to evaluate in vitro antioxidants activities. Furthermore, inhibition effect of extracts on α- amylase enzyme was carried out by using starch as a substrate, pancreatic α-amylase as the enzyme, and acarbose as standard.Results: Phytochemical screening of methanolic extract of all three selected plants displayed the presence of different chemical constituents such as alkaloids, polyphenols, flavonoids, terpenoids, saponins, glycosides, and tannins. The results of DPPH assay revealed that R. ellipticus and R. arboreum were most active with half maximal inhibitory concentration (IC50) values 33.41 μg/ml and 47.28 μg/ml, respectively. R. ellipticus was found to be effective toward α-amylase inhibition with IC50 values 269.94 μg/ml.Conclusion: The preliminary results of this study have put forward R. ellipticus into promising herbs with good antioxidant activities and α-amylase inhibition potential although further studies are needed to assess its mechanism of action.

ANALYSIS OF PHYTO-CONSTITUENTS, ANTIOXIDANT, AND ALPHA AMYLASE INHIBITORY ACTIVITIES OF PERSEA AMERICANA MILL., RHODODENDRON ARBORETUM SM. RUBUS ELLIPTICUS SM. FROM ARGHAKHANCHI DISTRICT NEPAL

Objective: To evaluate the phytochemical, antioxidant activities, and α-amylase inhibition assay for methanolic extract of three ethnomedicinal plants, namely Persea americana Mill., Rubus ellipticus Sm., and Rhododendron arboretum Sm. collected from Arghakhanchi District of Nepal using in vitro studies.Methods: Methanolic plant extracts were prepared by cold percolation method. Analysis of phytochemical constituents was carried out using standard methods. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to evaluate in vitro antioxidants activities. Furthermore, inhibition effect of extracts on α- amylase enzyme was carried out by using starch as a substrate, pancreatic α-amylase as the enzyme, and acarbose as standard.Results: Phytochemical screening of methanolic extract of all three selected plants displayed the presence of different chemical constituents such as alkaloids, polyphenols, flavonoids, terpenoids, saponins, glycosides, and tannins. The results of DPPH assay revealed that R. ellipticus and R. arboreum were most active with half maximal inhibitory concentration (IC50) values 33.41 μg/ml and 47.28 μg/ml, respectively. R. ellipticus was found to be effective toward α-amylase inhibition with IC50 values 269.94 μg/ml.Conclusion: The preliminary results of this study have put forward R. ellipticus into promising herbs with good antioxidant activities and α-amylase inhibition potential although further studies are needed to assess its mechanism of action.

Evaluation of the antidepressant activity of Tricholepis glaberrima bark alone and in combination with Mimosa pudica root extract

Background: The incidence of depressive disorder statistics has increased in every year; it differs from county to county and risk factor is also differed between country and lifestyle modification, cultures. Depression is a serious health problem in worldwide including India. Due to the present utilizing antidepressant drugs, a variety of upsetting symptoms. Amid the most recent decade, the disclosure of more secure stimulant natural cures is on the rise. Tricholepis glaberrima has been utilized in ancient people prescription different diseases incorporating central nervous system disorders in Ayurveda. Aims and Objectives: The study was aimed to investigate the persistent behavioral antidepressant activity of T. glaberrima bark alone and in combination with Mimosa pudica root extract. Materials and Methods: Ethanolic extract of T. glaberrima bark and M. pudica root extracts was prepared followed by analysis of phytochemical constituents was determined using appropriate biochemical method, acute toxicity studies also performed, and the biological investigation of both plants extracts was conducted through pharmacological screening models (forced swimming test and tail suspension test) in Swiss albino mice. Sixty trained animals were divided into six groups, each group six animals allotted (n = 6), Groups-1 and 2 served as vehicle control and imipramine (15 mg/kg), respectively, Groups-3 and 4 were treated 200 mg/kg and 400 mg/kg of Ethanolic extract of T. glaberrima bark (ETGB), and Group-5 was treated ETGB 100 mg/kg and Ethanolic extract of M. pudica root (EMPR) 100 mg/kg extracts; Imipramine was used as reference standard control. Following 14 days chronic treatment with acute restrain stress induced. All animals were tested using behaviors depression screening models. Results: The study shown significant (P < 0.0001 [at 1% level]) improvement in chronically treated mice in both screening models. Group V shown significant improvement compare to alone ETGB treated groups. Conclusion: The results suggest that this combination groups can be potential antidepressant effect. However, further mechanistic studies are required to confirm the cellular action and therapeutic efficacy of ETGB and EMPR against depression.

GC-MS Analysis of Phytochemical Constituents in Methanol Extract of Wood Bark from Durio Zibethinus Murr

GC-MS Analysis of Phytochemical Constituents in Methanol Extract of Wood Bark from Durio Zibethinus Murr

Analysis of Phytochemical Constituents and Biological Activity of Taxus Wallichiana Zucc. Dolakha District of Nepal

Phytochemical and biological activities of methanolic extract of Taxus wallichiana Zucc. (Leaf, stem) were carried out. The brine shrimp bioassay showed T. wallichiana is pharmacologically active. The antibacterial potential was studied against one gram positive bacteria (Staphylococcus aureus) and one gram negative bacteria (Escherichia Coli) using Agar Well Diffusion Method. Stem of T. wallichiana showed significant zone of inhibition against gram positive bacteria while the leaf of T. wallichiana did not show significant zone of inhibition against both gram positive and gram negative bacteria. Antioxidant activity was evaluated by 2, 2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging activity and FRAP assay. Both assay showed that T. wallichiana leaves has high antioxidant activities.Int. J. Appl. Sci. Biotechnol. Vol 6(2): 110-114