Nutrient Analysis Research Articles

Comparative assessment of levels and dietary intake of mineral nutrients and toxic metals in different varieties of teff from Hidabu Abote, Ethiopia

Ethiopia is the largest teff (Eragrostis teff Zucc) producer in the world. This research was intended to determine the levels of mineral nutrients and non-essential metals in the teff samples. After optimization, the samples were wet-digested using reagents (5 mL HNO3 and 1 mL HClO4), at a temperature of 230 °C for 2:30 h. Inductively coupled plasma optical emission spectrometry was used for the analysis of mineral nutrients and toxic metals in the teff samples. Under the optimum procedure, the coefficients of determination (R2) ranged between 0.9980 and 0.9999. The limits of detections (LODs) and limits of quantifications (LOQs) were in the range of 0.036–14.49 mg/kg and 0.111–43.93 mg/kg, respectively. The recovery ranged from 80.72 % to 107.79 %. The overall mean concentration in the three types of teff samples determined (mg/kg) was in the range of P (3890–4853), K (3040–3784), Ca (1906–1959), Mg (1402–1698), Fe (128–305), Mn (64–127.8), Na (50–136.5), Zn (19.8–27.3), B (1.8–21.9), Cu (4.17–6.9), Ni (2.6–4.05), Hg (1.8–4.0), Pb (0.048–3.7), Cd (0.012–2.09), As (0.02–0.24), with% RSD ranging between 0.017 and 11.1 %. The results obtained revealed that teff grain is a good source of minerals and contains a significant amount of toxic elements like Cd, Hg, As, and Pb.

Synergistic Benefits of Combined Application of Manures and Chemical Fertilizers: Enhancing Growth and Yield of Rice and Wheat

The incorporation of compost and manure with chemical fertilizer has become a key concern to sustain soil fertility. Because the nutrients are continuously depleting from the soil and consequently creating a risk to preserving soil health. Aims: This study assesses the response of different doses of cowdung (CD), municipal solid waste compost (MSWC), oil cake (OC), and poultry manure (PM), incorporated with fertilizers on the growth, yield and protein content of BRRI dhan49 rice and BARI Gom-24. Study Design: RCBD with 8 treatments and 3 replications. Place and Duration of Study: Field experiment and nutrient analysis were carried out at Bangladesh Agricultural University (BAU), Mymensingh. Wheat was grown during the rabi season from October 2018 to March 2019. Again, rice was cultivated during kharif II season from August 2019 to December 2019 on the same field. Then the laboratory analysis was performed till June 2020. Methodology: Eight treatments i.e., T0 Control (no fertilizer), T1 Recommended Fertilizer Dose, T2 (CD + NPK), T3 (MSWC + NPK), T4 (OC + NPK), T5 (PM + N), T6 (MSWC + N), T7: (CD + OC) were accommodated. Data on the growth parameters including grain and straw yields were documented after the harvest of the fully matured crop simultaneously determining the nitrogen (N) and protein content. Results: Adoption of various combinations of compost and manures with fertilizer accorded an increase in yield contributing characters as well as the N and protein content. Integrating MSWC @ 10 t ha-1+N projected the highest grain yield (7.11 t ha-1 in rice; 4.84 t ha-1 in wheat) and straw yield (8.8 t ha-1 in rice; 5.55 t ha-1 in wheat). It also promisingly secured the highest protein content compared to all other treatments. Conclusion: The appliance of MSWC @ 10 t ha-1 with the recommended dose of N may be endorsed for rice and wheat cultivation in the AEZ-9 region and retaining the physicochemical properties of Old Brahmaputra Floodplain soils.

FITNOURISH: Smart Nutrition Tracker for Fitness Enthusiasts

In today’s health-conscious society, individuals are increasingly recognizing the importance of nutrition in optimizing their overall health and wellness. However, the conventional approach to nutrition often fails to consider the unique needs and preferences of individuals, resulting in generic dietary recommendations that may not be effective or sustainable. The Personalized Nutrition Analyzer is a cutting-edge system designed to address this gap by providing tailored nutrition recommendations and meal planning services based on each user’s specific characteristics, dietary preferences, and health goals. By leveraging advanced technology and data analytics, this sophisticated system empowers individuals to make informed decisions about their dietary intake, ultimately contributing to improved health outcomes and enhanced well-being. The Personalized Nutrition Analyzer revolutionizes dietary guidance by offering tailored nutrition recommendations and meal planning services to individuals striving for optimal health and wellness. Through meticulous analysis of user input, dietary preferences, and health objectives, the system provides personalized meal plans, nutrient analyses, and insightful guidance to facilitate informed decision-making regarding dietary choices. In a world where health consciousness is paramount, traditional one-size-fits-all dietary approaches often overlook individual nuances, leading to suboptimal outcomes. However, the Personalized Nutrition Analyzer bridges this gap by leveraging sophisticated technology and data analytics to customize dietary strategies according to each user’s unique characteristics and goals. By empowering users with personalized insights and actionable recommendations, the system promotes sustainable lifestyle changes and fosters improved health outcomes

Metabolome and Transcriptome Association Analysis Reveals Mechanism of Synthesis of Nutrient Composition in Quinoa (Chenopodium quinoa Willd.) Seeds.

Quinoa (Chenopodium quinoa Willd.) seeds are rich in nutrition, superior to other grains, and have a high market value. However, the biosynthesis mechanisms of protein, starch, and lipid in quinoa grain are still unclear. The objective of this study was to ascertain the nutritional constituents of white, yellow, red, and black quinoa seeds and to employ a multi-omics approach to analyze the synthesis mechanisms of these nutrients. The findings are intended to furnish a theoretical foundation and technical support for the biological breeding of quinoa in China. In this study, the nutritional analysis of white, yellow, red, and black quinoa seeds from the same area showed that the nutritional contents of the quinoa seeds were significantly different, and the protein content increased with the deepening of color. The protein content of black quinoa was the highest (16.1 g/100 g) and the lipid content was the lowest (2.7 g/100 g), among which, linoleic acid was the main fatty acid. A combined transcriptome and metabolome analysis exhibited that differentially expressed genes were enriched in "linoleic acid metabolism", "unsaturated fatty acid biosynthesis", and "amino acid biosynthesis". We mainly identified seven genes involved in starch synthesis (LOC110716805, LOC110722789, LOC110738785, LOC110720405, LOC110730081, LOC110692055, and LOC110732328); five genes involved in lipid synthesis (LOC110701563, LOC110699636, LOC110709273, LOC110715590, and LOC110728838); and nine genes involved in protein synthesis (LOC110710842, LOC110720003, LOC110687170, LOC110716004, LOC110702086, LOC110724454 LOC110724577, LOC110704171, and LOC110686607). The data presented in this study based on nutrient, transcriptome, and metabolome analyses contribute to an enhanced understanding of the genetic regulation of seed quality traits in quinoa, and provide candidate genes for further genetic improvements to improve the nutritional value of quinoa seeds.

Investigations on Xenopus laevis body composition and feeding behavior in a laboratory setting

The African clawed frog, Xenopus laevis, has been used as a laboratory animal for decades in many research areas. However, there is a lack of knowledge about the nutritional physiology of this amphibian species and the feeding regimen is not standardized. The aim of the present study was to get more insights into the nutrient metabolism and feeding behavior of the frogs. In Trial 1, adult female X. laevis were fed either a Xenopus diet or a fish feed. After 4 weeks, they were euthanized, weighed, measured for morphometrics and dissected for organ weights and whole-body nutrient analysis. There were no significant differences between the diet groups regarding the allometric data and nutrient contents. The ovary was the major determinant of body weight. Body fat content increased with body weight as indicator of energy reserves. In Trial 2, 40 adult female frogs were monitored with a specifically developed digital tracking system to generate heat-maps of their activity before and up to 25 min after a meal. Three diets (floating, sinking, floating & sinking) were used. The main feed intake activity was fanning the feed into the mouth, peaking until 20 min after the meal. The different swimming characteristics of the diets thereby influenced the activity of the animals. Our dataset helps to adjust the feeding needs to the physical composition and also to meet the natural behavioral patterns of feed intake as a prerequisite of animal wellbeing and animal welfare in a laboratory setting.

Assessment of foliar application of nutrients on yield and quality of guava (Psidium guajava)

Present study was carried out during rainy (kharif) seasons of 2020–2022 at CCS Haryana Agricultural University, Hisar, Haryana to assess the effect of foliar application of nutrients on the yield and quality aspects of rainy season guava (Psidium guajava L.). Experiment was conducted in a randomized block design (RBD) comprised of 4 different foliar applications of zinc sulphate, viz. ZnSO4 @0% (Control); ZnSO4 @0.3%; ZnSO4 @0.6%; ZnSO4 @0.9%; and 4 foliar applications of potassium sulphate, viz. K2SO4 @0% (Control); K2SO4 @1.0%; K2SO4 @1.5%; and K2SO4 @2.0, and replicated thrice. However, foliar application of ZnSO4 @0.9% was found to be the best treatment in improving the fruit yield (46.2 kg/plant), fruit weight (135.2 g), fruit length (6.10 cm) and fruit breadth (5.64 cm) of rainy season guava (cv. L-49). Similarly, ZnSO4 @0.6% was found best for increasing the TSS (total soluble solids) (10.6 B) and ascorbic acid content (187.4 mg/100 g pulp). On the other hand, K2SO4 @2.0% increased the fruit yield (45.4 kg/plant), fruit weight (135.2 g) and ascorbic acid content (190.3 mg/100 g pulp) of guava fruit. The maximum N, P, K and Zn content for nutrient analysis in leaf was noted under foliar application of ZnSO4 @0.9% and K2SO4 @2.0%. The maximum N and Zn content in fruit was observed due to application of ZnSO4 @0.9% and maximum P and K content was observed with treatment ZnSO4 @0.6%. Similarly, K2SO4 @2.0% increased the N, P, K and Zn content of guava fruit.

Comprehensive Analysis of Biomass, Nutrient, and Heavy Metal Contributions of Pelagic Sargassum Species (Phaeophyceae) Inundations in South Florida

Pelagic Sargassum landings (hereby referred to as sargasso) increased dramatically in 2011 throughout the equatorial tropical Atlantic due to the formation of the Great Atlantic Sargassum Belt (GASB). Despite increasing reports, understanding of local abundances and vegetative characteristics, especially in South Florida, remains limited. From 2018 to 2021, sargasso was collected at two South Florida beaches, with additional sampling at a third beach to assess nutrient and heavy metal concentrations. Biomass landings varied greatly, with S. fluitans III predominant during the “peak season” (May to July) and S. natans I predominant in the “off season”, while S. natans VIII was consistently least abundant. This suggests that South Florida may receive sargasso from the Sargasso Sea during the low season and from the GASB during the peak sargasso season. Across all three morphotypes, mean nitrogen (N) and phosphorus (P) contents were 0.97% and 0.04% (dry weight), respectively. Out of the 16 heavy metals detected, our values were similar to those reported across the Caribbean. Arsenic was the most prevalent heavy metal, with sargasso containing epibionts having higher arsenic concentrations. These results provide comprehensive information to better understand the characteristics and potential origin of sargasso landings in South Florida.

Total fermentable oligo-, di-, monosaccharides and polyols intake, carbohydrate malabsorption and gastrointestinal symptoms during a 56 km trail ultramarathon event.

To explore the relationship between nutritional intake, fermentable oligo-, di, monosaccharides and polyols, and carbohydrate malabsorption, with gastrointestinal symptoms during a 56 km trail ultramarathon event and identify differences in nutritional intake between runners with severe and non-severe gastrointestinal symptoms. Forty-four ultramarathoners recorded and self-reported dietary intake 3 days before, morning of, and during the ultramarathon with gastrointestinal symptoms obtained retrospectively and nutrient analysis via FoodWorks. Carbohydrate malabsorption was determined via breath hydrogen content pre- and post-race. Spearman's rank-order and Mann-Whitney U-tests were used to identify relationships and differences between groups. Total fermentable oligo-, di, monosaccharides and polyols intake were not associated with gastrointestinal symptoms, but weak associations were observed for lower energy (rs = -0.302, p = 0.044) and fat intake (rs = -0.340, p = 0.024) 3 days before with upper gastrointestinal symptoms and higher caffeine intake 3 days before with overall gastrointestinal symptoms (rs = 0.314, p = 0.038). Total fermentable oligo-, di-, monosaccharides and polyols intake and breath hydrogen were not different between those with severe versus non-severe symptoms (p > 0.05). Although those with severe symptoms had higher caffeine (p = 0.032), and total polyols intake (p = 0.031) 3 days before, and higher % energy from fat (p = 0.043) and sorbitol intake (p = 0.026) during the race, and slower ultramarathon finish times (p = 0.042). Total fermentable oligo-, di-, and monosaccharides intake and carbohydrate malabsorption were not associated with gastrointestinal symptoms. Additional research on the effect of fat, caffeine, and polyol intake on exercise-associated gastrointestinal symptoms is warranted and presents new nutritional areas for consideration when planning nutritional intake for ultramarathoners.

Investigating the Impacts of Wastewaters on Lettuce (Lactuca sativa) Seed Germination and Growth

There is an opportunity for agriculture to utilize the many different waste streams in our world and capitalize on what would otherwise be viewed as waste products. Hydrothermal liquefaction (HTL) is an emerging technology for converting wet biomass to bio-crude oil, while aquaponics is a practice tracing back to indigenous communities around the world; both technologies have the potential to sustainably provide the necessary nutrients for crop growth. Food systems worldwide are actively transitioning to address the many challenges of climate change in a sustainable and efficient manner. Urban agriculture (UA) has the potential to generate localized crops in densely populated areas year-round, but has its challenges, involving high capital requirements, especially for vertical farming and controlled-environment agriculture, and being energy intensive due to artificial lighting and fossil fuel-based synthetic fertilizers. This study investigated the potential for aquaponic and HTL effluents to be used in hydroponic systems through a seed germination screening experiment. Buttercrunch lettuce (Lactuca sativa L.) seeds were placed in Ziploc plastic bags on paper towels saturated with the wastewater treatments for 10 days while their total length of growth was routinely measured from the tip of the root to the tip of the cotyledons. The Chicago High School for Agricultural Sciences (CHSAS) aquaponic effluent with a 5.8× times higher nitrate concentration and 4.25× higher ammonia concentration outperformed the Bevier aquaponic effluent and improved any other source water it was combined with. Results also showed that seed germination was not inhibited in the presence of 2–8% solutions of hydrothermal liquefaction aqueous phase (HTL-AP), which performed on par with standard hydroponic fertilizer; solutions of a higher percentage, though, may lead to inhibitory effects in plants, and those of a lower percentage may not provide enough nutrients in the proper forms to sustain plant growth. However, the nutrient analyses revealed that there is still much to investigate regarding the combination of wastewaters to provide a complete, well-rounded, and sustainable source for hydroponic crop production.

Effects of Neonicotinoid Seed Treatments on Cotton Seedling Physiology, Nutrition, and Growth

Plant growth and physiology can be affected by environmental and chemical factors that have the potential to influence yields. Among the factors that influence plant growth, neonicotinoid seed treatments have shown significant effects on plant growth, particularly in cotton. The dual benefits seen from neonicotinoids on plant growth and insect control show promise in improving cotton yields but little is known about how different seed treatments affect seedling physiology. A greenhouse experiment was undertaken to investigate how three neonicotinoid seed treatments (clothianidin, thiamethoxam, and imidacloprid) affect the physiology and growth of cotton seedlings in controlled environmental conditions. A randomized complete block design was used to examine seed treatments and an untreated control. Cotton seeds were treated, grown, and evaluated for physiological changes until the fifth true leaf-stage and measurements were taken at each of these stages. Data were collected on plant height, shoot fresh weight, leaf area, root length, and root biomass. In addition, chlorophyll pigments and nutrient analysis were performed on cotton seedlings. The seedlings of imidacloprid treated seeds had greater height, shoot fresh mass, leaf area, and relative growth rate by the fifth true leaf stage compared to other treated plants; however, clothianidin showed comparative performance at earlier stages in plant development that equilibrated over time. While all neonicotinoid seed treatments showed positive effects, imidacloprid showed the most potential as a bioactivator on plant growth.

Physical and chemical features of hydro-geologically connected wetlands in the Abbay River basin, Ethiopia

Physico-chemical water quality assessment is vital for wetland characterization, detecting environmental stress affecting the ecosystem and wetland management interventions. Therefore, this study aimed at comprehensive analysis of water quality parameters of six hydro-geologically connected wetlands in West Gojjam zone, Amhara region, Ethiopia. Water quality data was collected for one year (from June 2022 to April 2023) during four sampling seasons (pre-rainy, main-rainy, post-rainy and dry seasons) from twelve purposively selected sampling sites. In-situ measurements and nutrient analysis were carried out following standard procedures. Water quality index (WQI) was also computed by the weighted arithmetic water quality index method (WAWQI) based on selected parameters. Phosphate ranged from 0.24 mg/l (Kurt Bahir) to 1.80 mg/l (Zindib) and nitrate ranged from 2.12 mg/l (at Gudera) to 3.25 mg/l (at Geray). This increased nutrient concentration and the decreasing trend in DO level clearly indicates their ecological degradation. There was a statistically significant difference (P < 0.05, One-way ANOVA) in the mean measurements of some of the parameters among the study wetlands and among the study seasons. The PCA also clearly shows the most influential variables responsible for the variation among the wetlands. The study concluded that the wetlands water quality has been degraded. Therefore, regular water quality monitoring is recommended to reduce further deterioration.

Analysis of Leaf and Soil Nutrients, Microorganisms and Metabolome in the Growth Period of Idesia polycarpa Maxim.

Idesia polycarpa Maxim is an emerging oil plant species. Understanding its microecological characteristics and internal mechanisms can serve as a basis for field management and the screening and application of growth-promoting bacteria during the growth phase of young trees. This study used three-year-old young I. polycarpa to analyze the tree's root morphology, soil, and leaf nutrient status variations from May to October. In addition, differences in the rhizosphere soil, leaf metabolites, and microorganisms were observed. The results showed that, from May to October, the total nitrogen (TN) in the soil significantly decreased, whereas the TN, total potassium (TK), and total phosphorus (TP) in the leaves differed (p < 0.05). The leaf-dominant bacteria changed from Pseudomonadota to Firmicutes phylum. In addition, the relative abundance of soil and leaf-dominant bacteria decreased. The study found that the soil and leaf differential metabolites were mainly sugars and phenolic acids. The soil bacterial community showed a significant correlation with soil pH, available potassium (AK), available phosphorus (AP), and TN (p < 0.05). Further, the soil fungal community was significantly correlated with pH and AK (p < 0.001). TP, pH, and TK were the main factors influencing the leaf bacterial community, while the leaf fungal community was significantly correlated with five factors, including pH, TC, and TN. The root morphology was also mainly affected by pH, Pedomicrobium sp., Talaromyces sp., Penicillium sp., and D-Mannitol 2.

Nutrition survey methods and food composition database update of the Korean Genome and Epidemiology Study.

This study presents the nutrition survey methods and the updated food composition database for the Korean Genome and Epidemiology Study (KoGES). The KoGES, which is the largest and longest cohort study in Korea, aims to identify genetic and environmental factors associated with chronic diseases. This study has collected dietary data using a validated semi-quantitative food frequency questionnaire and/or the 24-hour recall method. However, these dietary survey methods use different food composition databases, and their nutritional values are out of date. Therefore, it became necessary to update the food composition database by revising nutrient analysis values to reflect improvements in the performance of food ingredient analysis equipment, revising international values to analysis values of Korean agricultural products, adjusting nutrient units, and adding newly reported nutrients related to chronic diseases. For this purpose, we integrated the different food composition databases used in each nutrition survey, updated 23 nutrients, and expanded 48 new nutrients for 3,648 food items using the latest reliable food composition databases published by national and international institutions. This revised food composition database may help to clarify the relationship between various nutrients and chronic diseases. It could serve as a valuable resource for nutritional, epidemiological, and genomic research and provide a basis for determining public health policies.

The sensory and nutrient analysis by the addition of Chia Seed (Salvia hispanica L.) to Star fruit juice (Averrhoa carambola L.) as a drink for hypertension patients

&lt;p&gt;&lt;strong&gt;&lt;em&gt;Background: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;Hypertension, when it is not controlled, can cause complications to many diseases and death. Hypertension control can be achieved by adjusting to a healthy diet, such as increasing the consumption of fruit, whole grains, potassium intake, calcium, and magnesium. Several studies have shown that star fruit and chia seeds can control blood pressure in people with hypertension.&lt;/em&gt; &lt;em&gt;However, the nutritional content, such as calcium and magnesium, is still relatively low in star fruit.&lt;/em&gt; &lt;em&gt;According to &lt;/em&gt;&lt;em&gt;USDA&lt;/em&gt;&lt;em&gt;, chia seeds contain high level of calcium, potassium, and magnesium.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Objectives: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;This research aims to determine the sensory acceptability and the nutritional content of star fruit juice by adding chia seeds.&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Methods: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;This is an experimental study with a Completely Randomized Design (CRD). There were four formulations of star fruit juice with chia seeds additions (0 g,10 g,15 g, and 20 g).&lt;/em&gt; &lt;em&gt;The sensory analysis was tested by the Kruskal Wallis test and Mann-Whitney test. While differences of the nutritional content from the product that preferred by panelists were tested by Independent T-test.&lt;/em&gt;&lt;em&gt;&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Results: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;There were significant differences (p&amp;lt;0.05) in all sensory parameters. The selected sweet star fruit juice with the addition 10 g chia seed formulation showed an increase in nutrients except for the water content. Water content of 89.21 %, ash content of 0.32 %, carbohydrate content of 7.14 %, fat content of 1.75 %, protein content of 1.55%, potassium content of 206 mg/200 ml, calcium content of 34.84 mg/200 ml, magnesium content of 17.80 mg/200 ml, and sodium content of 2.46 mg/200 ml.&lt;/em&gt;&lt;/p&gt;&lt;p&gt;&lt;strong&gt;&lt;em&gt;Conclusions: &lt;/em&gt;&lt;/strong&gt;&lt;em&gt;Sweet start fruit juice with the addition of 10 g of chia seed is the most liked star fruit juice formulation with chia seed addition by the research panellists, and it can increase the nutritional content, especially calcium and magnesium content.&lt;/em&gt;&lt;/p&gt;

The GUTFIT Cohort: Identifying dietary intake of Chinese New Zealanders with functional constipation

Distinct pathophysiology has been identified with disorders of gut-brain interactions (DGBI), including functional constipation (FC)(1,2), yet the causes remain unclear. Identifying how modifiable factors (i.e., diet) differ depending on gastrointestinal health status is important to understand relationships between dietary intake, pathophysiology, and disease burden of FC. Given that dietary choices are culturally influenced, understanding ethnicity-specific diets of individuals with FC is key to informing appropriate symptom management and prevention strategies. Despite distinct genetic and cultural features of Chinese populations with increasing FC incidence(3), DGBI characteristics are primarily described in Caucasian populations(2). We therefore aimed to identify how dietary intake of Chinese individuals with FC differs to non-Chinese individuals with FC, relative to healthy controls. The Gastrointestinal Understanding of Functional Constipation In an Urban Chinese and Urban non-Chinese New Zealander Cohort (GUTFIT) study was a longitudinal case-control study using systems biology to investigate the multi-factorial aetiology of FC. Here we conducted a cross-sectional dietary intake assessment, comparing Chinese individuals with FC (Ch-FC) against three control groups: a) non-Chinese with FC (NCh-FC) b) Chinese without FC (Ch-CON) and c) non-Chinese without FC (NCh-CON). Recruitment from Auckland, New Zealand (NZ) identified Chinese individuals based on self-identification alongside both parents self-identifying as Chinese, and FC using the ROME IV criteria. Dietary intake was captured using 3-day food diaries recorded on consecutive days, including one weekend day. Nutrient analysis was performed by Foodworks 10 and statistical analysis with SPSS using a generalised linear model (ethnicity and FC status as fixed factors). Of 78 enrolled participants, 66 completed the study and 64 (39.4 ± 9.2 years) completed a 3-day food diary at the baseline assessment. More participants were female (84%) than male (16%). FC and ethnicity status allocated participants into 1 of 4 groups: Ch-FC (n = 11), Ch-CON (n = 18), NCh-FC (n = 16), NCh-CON (n = 19). Within NCh, ethnicities included NZ European (30%), non-Chinese Asian (11%), Other European (11%), and Latin American (2%). Fibre intake did not differ between Ch-FC and NCh-FC (ethnicity × FC status interaction p&gt;0.05) but was independently lower overall for FC than CON individuals (21.8 ± 8.7 versus 27.0 ± 9.7 g, p&lt;0.05) and overall for Ch than NCh (22.1 ± 8.0 versus 27.0 ± 10.4 g, p&lt;0.05). Carbohydrate, protein, and fat intakes were not different across groups (p&gt;0.05 each, respectively). In the context of fibre and macronutrient intake, there is no difference between Ch-FC and NCh-FC. Therefore, fibre and macronutrients are unlikely to contribute to potential pathophysiological differences in FC between ethnic groups. A more detailed assessment of dietary intake concerning micronutrients, types of fibre, or food choices may be indicated to ascertain whether other dietary differences exist.

In-Depth Analysis of Diet Diary and Urine pH Measurements Improved Food Diet Reporting in Postmenopausal Women with RUTI.

We proceeded with an in-depth review of diet diaries for nutrient analysis from a cohort of women suffering from uncomplicated recurrent urinary tract infection (RUTI) to validate the accuracy of our current food diet record (FDR) form and evaluate possible domains of improvement. As part of an IRB-approved study, this previously published cohort of NHANES-comparable women was analyzed for consistency of the nutrient intake over 3 days, the influence of the time between meals and urine pH, the effect of nutrient intake over interval time between meals, and seasonal and before/during the COVID-19 pandemic changes. Intrarater reliability for nutrient analysis and intrapatient variability for urine pH were computed to test for consistency. Intrarater reliability for diet analysis was 91% accurate for foods and beverage matching and nutrient analysis. Mean standard deviation of urine pH readings within study participants was 0.4 (95% CI: 0.4, 0.5). An association was noted between total calories and fat consumed at breakfast and an increase in time to lunch. Calories consumed were unaffected across seasons or during the COVID-19 pandemic. Water intake during summer was significantly lower than that during fall and winter (both, p < 0.001). The patients who reported drinking water had a significantly lower average urine pH than women who did not report drinking water (5.8 vs. 6.2; p = 0.026). In this cohort of postmenopausal women with RUTIs, in-depth analysis of our current FDR findings led to several actionable items, which will improve our current food diet self-reporting process by our patients.

A nutritional modelling framework for inclusion in a Norwegian food system model

The aim of this study was to apply a comprehensive mathematical model designed to assess the current and future availability of food, as well as the resulting nutrient availability and intake, in the context of the Norwegian population. The model explores various scenarios, including self-sufficiency levels for food supply and impacts on nutrition recommendations. A food mass balance charting production, import, export, feed, seed, and consumer allocation in the Norwegian food system provided insights into the nutrients available to the population. The analysis included a comparison with two versions of the Nordic Nutrient Recommendations (NNR)1, with the latest version recommending substantial changes in food consumption compared to current dietary patterns in Norway. The nutrient analysis compared the food mass to Matvaretabellen2 and was supplemented with data from the United Nations World Population Prospects3 and the Food and Agriculture Organisation/World Health Organisation/United Nations4 report to ensure comprehensive nutrient analysis. Micronutrient gaps were observed in Iodine (94% of the target intake) and Vitamin D (46% of the target intake), while saturated-fatty acids slightly exceeded the recommended requirements (107% of the upper limit) based on the current baseline scenario. The updated NNR4 recommends changes to specific food categories, namely fruits, vegetables, nuts, and seeds. A secondary scenario testing compared against the updated NNR found that increasing the availability of the supply of these groups does not result in any new nutrient gaps, demonstrating the feasibility of addressing the issue on a national supply basis. These approach offers a mathematical-modelling based tool that can be used to provide information for a national food system. Leveraging the model’s capacity to simulate various scenarios, informed decisions to optimise self-sufficiency levels and align food supply with recommended nutritional guidelines can be made. To improve the model, higher data resolution and clearer categorisation of food groups are required which can then be linked into a more complete national food system. The mathematical model presented in this study provides a framework for understanding of food and nutrient availability in Norway. By identifying critical nutrient gaps and potential solutions, this research contributes knowledge for a healthier and sustainable food future for the nation.

Enhancing Freeze Tolerance of Field-grown Lettuce with Salicylic Acid, Ascorbic Acid, and Calcium Chloride

With the climate continuing to change, specialty crop growers are particularly at risk for economic loss caused by erratic weather conditions, excess heat and rain, and frost damage. Although tolerant to cold, lettuce (Lactuca sativa) is a major horticultural crop at risk for exposure to freezing temperatures in late fall or early spring in the Midwest. Exogenous applications of salicylic acid, ascorbic acid, and calcium chloride have been shown to improve abiotic stress in plants, particularly with freezing tolerance. This research investigated the effects of the exogenous application of salicylic acid, ascorbic acid, and calcium chloride, in varying concentrations, on field-grown lettuce. The study was conducted at the Iowa State University Horticulture Research Station, Ames, IA, USA. Weekly applications of salicylic acid (0.5 and 1.0 mM), ascorbic acid (0.5 and 1.0 mM), and calcium chloride (10 and 20 mM) were applied until plants were a marketable size. A control treatment (no spray) was also included. Data regarding plant size, yield, leaf area, leaf number, plant dry weight, plant nutrient analysis, and freeze tolerance were collected. Freeze tolerance assessments were conducted through laboratory-simulated freeze events and the evaluation of natural in-field freeze events. Freeze injury was quantified through the electrolyte leakage method. Marketable weights in 2020 and 2021 were statistically similar, except for 1.0 mM salicylic acid in 2020, which showed a significantly lower marketable weight and head diameter. Trends during both years showed that stress protectant applications had the most effective freeze protection at −12 °C in laboratory-simulated freeze events. Calcium chloride at 20 mM had the highest protection at −12 °C, with 13.3% and 24.0% less injury compared with the control in 2020 and 2021, respectively. Salicylic acid 1.0 mM at −12 °C had 4.7% and 23.7% less injury compared with control during these two years, respectively. Ascorbic acid 1.0 mM at −12 °C also showed 6.8% and 9.5% less injury than the control in 2020 and 2021, respectively. Similar trends were observed after in-field freezing events, with 20 mM calcium chloride providing the highest protection against frost in 2020 and 2021. These findings advance the understanding of the capabilities of stress protectants on lettuce as chemical primers after freezing events. This research highlights the benefits of ascorbic acid, salicylic acid, and calcium chloride as stress protectants and encourages future research and further exploration of concentrations, methods of application, and timing of application of products.

Development of Gluten-Free Bread from Brown Rice-Potato Starch Composite Flour Incorporated with Red Seaweed Kappaphycus alvarezii powder

Gluten free diets and lifestyles are rapidly increasing in popularity mainly due to the increase in consumer awareness about gluten related disorders and gluten intolerances. With increasing demand, the food industry strives to develop new food products that are gluten-free and able to be consumed by those who suffer from these conditions. The objective of this study was to develop a gluten-free bread from brown rice-potato starch composite flour incorporated with red seaweed Kappaphycus alvarezii (KA) powder. KA powder, ranging from 2% to 10%, was added, and the physical properties of the gluten- free bread were assessed. Sensory evaluation was conducted using two methods, the descriptive and hedonic tests. The amount of seaweed significantly (p&lt;0.05) influenced the loaf volume, with F5 (10%), the highest concentration of seaweed, having the highest reduction of volume of 15.38% compared to the control F0 (without seaweed). With increasing seaweed concentration, the brightness and yellowness of each bread decreased. The texture profiles of KA-incorporated bread formulations were also affected with the firmness of bread being the most significant change. The descriptive test showed that F5 (10%) had the highest intensity of seaweed effect which affected the colour, texture, aroma, taste and aftertaste of the bread. The hedonic test showed that the most acceptable formulation was F1 (2%). From these formulations, the best formulation F1 (2%) was chosen for further nutrient analysis where it was compared to the control (without seaweed). It was shown that F1 had significantly (p&lt;0.05) higher moisture, ash, fat, and dietary fibre content, but lower carbohydrate and protein content compared to F0 (without seaweed). In conclusion, the formulation of gluten-free KA- incorporated brown rice-potato starch bread had an impact on the bread texture, colour, volume, and nutrient content.

NPK NUTRIENT STATUS IN SOIL AND LEAVES OF PIPER NIGRUM L. UNDER DIFFERENT GROWTH CRITERIA

The pepper plant (Piper nigrum L.) is Indonesia's leading spice commodity. Bangka Belitung as a producing center still has problems in cultivation, including nutrient availability. Nutrients N, P and K are macro elements that really determine the growth of plants, including pepper. This research aims to identify the status of macro nutrients, namely N, P and K in pepper plants in Simpang Katis Village, Central Bangka Regency, through observing growth status, leaf visuals, nutrient analysis and plant tissue analysis. This research was carried out in Simpang Katis Village, Central Bangka Regency in July-September 2023. The experimental method used in this research was observation and laboratory analysis methods. The results of the observation data show that NPK nutrients in land with very good growth criteria have N-total of 0.22%, P2O5 available is; 383.2 mg/kg and available K2O of; 6.4 mg/kg. Plant growth with poor growth criteria has a total N content of 0.20%, P2O5 of 115.1 mg/kg and K20 of 7.1 mg/kg. kg. while plant tissue has an N-Total content of 2.41%, P-Total of 0.26 percent and K-total of 2.47 percent for plants with very good growth criteria and N-total, P-total and K-total respectively - respectively .2.79, 0.32 and 3.95 mg/kg for plant tissue with poor growth criteria.