Analysis Of Fourier Transform Infrared Spectra Research Articles

Effects of treatment methods on the formation of resistant starch in purple sweet potato

In order to determine the mechanisms underlying resistant starch formation, three treatments were used to prepare resistant starch from purple sweet potato. The resistant starch yield, amylose content, chain length distribution, thermal properties, and crystal structure were determined, and the results were compared with those of unmodified starch. Autoclaving, pullulanase, and pullulanase-autoclaving treatments significantly increased the resistant starch yield, amylose content, shorter amylopectin branch content, and gelatinisation temperatures of native purple sweet potato starch. Resistant starch prepared via pullulanase-autoclaving combination treatment exhibited the highest gelatinisation enthalpy value and the greatest degree of overall thermal stability. X-ray diffraction patterns and Fourier-transform infrared spectra analysis demonstrated that all three treatments transformed the starch crystalline structure from C-type to B-type, and no new groups were generated during the modification process; all the processes were only physical modifications.

Preparation and characterization of high strength and high modulusPVAfiber via dry‐wet spinning with cross‐linking of boric acid

AbstractTo improve the mechanical properties of polyvinyl alcohol (PVA) fibers, a series of PVA fibers were prepared via dry‐wet spinning with cross‐linking of boric acid (BA) (PVA/BA fibers), and using the mixed solvent of dimethyl sulfoxide and water. Moreover, the final PVA/BA fibers were characterized by Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), differential scanning calorimetery (DSC), thermogravimetric analyzer (TGA), powder X‐ray diffraction (XRD) and yarn strength tester. Furthermore, with the increasing of BA content, FTIR analysis showed that the degree of crosslinking of BA with PVA increased. SEM images of final PVA/BA fibers presented smooth surfaces, and the diameters decreased firstly and then increased. DSC, TGA, and XRD analysis indicated that the melting temperatures, thermal properties and crystallinities first increased and then decreased with the increasing of BA content. In addition, mechanical properties measurements illustrated that the cross‐linking existed at an optimal BA content of 0.3 wt%, and PVA/BA‐0.3 fiber had the highest tensile strength and Young's modulus of 13.1 ± 0.4 and 360.2 ± 10.4 cN/dtex, respectively.

Deep-sea mercury resistant bacteria from the Central Indian Ocean: A potential candidate for mercury bioremediation

Deep-sea bacteria when grown in normal environmental conditions get morphologically and genetically adapted to resist the provided culture conditions for their survival, making them a possible aspirant in mercury bioremediation. In this study, seawater samples were collected from different depths of the Central Indian Ocean and seven mercury resistant bacteria (resistant to 100 mg L-1 concentration of inorganic Hg as HgCl2) were isolated. Based on 16S rRNA gene sequencing, the identified isolates belong to the genera Pseudomonas, Bacillus and Pseudoalteromonas. The presence of the merA gene in the isolates contributes to the effective volatilization of mercury. The Inductively Coupled Plasma Mass-Spectroscopy analysis revealed that the isolates can reduce up to >80% of inorganic mercury. Moreover, Fourier Transform Infrared spectrum analysis indicates that functional groups play a key role in the mechanism of adaptation towards Hg2+ reduction. Thus, the deep-sea bacteria expressed significant tolerance and reduction potential towards ionic mercury.

Iso-mukaadial acetate and ursolic acid acetate inhibit the chaperone activity of Plasmodium falciparum heat shock protein 70-1.

Plasmodium falciparum is the most lethal malaria parasite. The present study investigates the interaction capabilities of select plant derivatives, iso-mukaadial acetate (IMA) and ursolic acid acetate (UAA), against P. falciparum Hsp70-1 (PfHsp70-1) using in vitro approaches. PfHsp70-1 facilitates protein folding in the parasite and is deemed a prospective antimalarial drug target. Recombinant PfHsp70-1 protein was expressed in E. coli BL21 cells and homogeneously purified by affinity chromatography. The interaction between the compounds and PfHsp70-1 was evaluated using malate dehydrogenase (MDH), and luciferase aggregation assay, ATPase activity assay, and Fourier transform infrared (FTIR). PfHsp70-1 prevented the heat-induced aggregation of MDH and luciferase. However, the PfHsp70-1 chaperone role was inhibited by IMA or UAA, leading to both MDH and luciferase's thermal aggregation. The basal ATPase activity of PfHsp70-1 (0.121 nmol/min/mg) was closer to UAA (0.131 nmol/min/mg) (p = 0.0675) at 5 mM compound concentration, suggesting that UAA has no effect on PfHsp70-1 ATPase activity. However, ATPase activity inhibition was similar between IMA (0.068 nmol/min/mg) (p < 0.0001) and polymyxin B (0.083 nmol/min/mg) (p < 0.0001). The lesser the Pi values, the lesser ATP hydrolysis observed due to compound binding to the ATPase domain. FTIR spectra analysis of IMA and UAA resulted in PfHsp70-1 structural alteration for β-sheets shifting the amide I band from 1637 cm-1 to 1639 cm-1, and for α-helix from 1650 cm-1 to 1652 cm-1, therefore depicting secondary structural changes with an increase in secondary structure percentage suggesting that these compounds interact with PfHsp70-1.

Behavior of molybdenum lowly doped SiO2–CaO–P2O5 composite interfacing a simulated biologic medium with serum albumin

This study is focussed on the biocompatibility evaluation by probing the affinity of bovine serum albumin (BSA) towards the surface of a silica based material containing CaMoO4 and hydroxyapatite nanocrystals. The adsorption occurring upon BSA interaction with the sample surface was investigated by Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) Spectroscopy and X-ray Photoelectron Spectroscopy (XPS). The elemental composition and evaluation of protein surface coverage based on information from the outermost few nanometres surface layer was achieved by XPS analysis. The ratio between carbon and nitrogen on the material surface after incubation in BSA solution was found to be reasonable close to the value obtained from the measurement of pure BSA, that proves the protein adsorption. The comparative analysis of FTIR spectra recorded from lyophilized protein and from biomaterial surface after protein adsorption was considered in order to obtain information about the secondary structure of the proteins. The protein surface coverage was also confirmed by SEM morphological characterization. The results obtained by in vitro test indicate that the nanostructured SiO2–CaO–P2O5−MoO3 system has a great potential for bone tissue engineering with respect to the ability to bind to serum protein.

Production of lipopeptide biosurfactant by Kurthia gibsonii KH2 and their synergistic action in biodecolourisation of textile wastewater

Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to produce biosurfactant and investigate the synergistic action on decolourisation of textile dyes by the combination of bacteria and biosurfactant. An effective dye degrading strain and biosurfactant-producer, Kurthia gibsonii KH2, was isolated from textile wastewater using molasses as the only source of carbon and energy. The isolates were identified and screened for biosurfactant production using haemolytic activity, oil spreading technique, drop collapse test and emulsification index. Fourier Transform Infrared Spectrum (FTIR) and Thin Layer Chromatography (TLC) analyses were carried out to detect the type of biosurfactant. The effect of different physicochemical parameters on textile wastewater decolourisation was assessed within 24 h. The Kurthia gibsonii KH2 showed positive results for haemolytic activity, oil spreading technique, and drop collapse test. The emulsification test (E24) revealed that Kurthia gibsonii KH2 had a higher emulsification index of 63%. FTIR and TLC analyses indicated that the biosurfactant was a lipopeptide and was formed with a yield of 2 gL −1 . The synergistic activity of Kurthia gibsonii KH2 and lipopeptide biosurfactant resulted in decolourisation levels of 85% at 100 mg/L concentration and pH 7 was recorded at 168 h of incubation. The high attributes of these combinations and the phytotoxicity tests implied that the metabolites were less toxic, making it a promising option for the biodecolourisation and biodegradation of industrial textile wastewater and various environmental conditions. • Textile wastewater treatment using utilisation of microbial biocatalysts is vital. • The lipopeptide biosurfactant was derived from the bacteria Kurthia gibsonii KH2. • The strain showed synergistic action in textile dyes removal efficiency. • Decolorisation process was the dominant removal mechanism.

The Characteristic of Gelatin Extracted from The Skin of Adult and Sub-Adult Striped Catfish (Pangasius hypophthalmus) Using Acid-Base Pretreatment: pH and FTIR

The gelatin is the biomaterial that produced by collagen denaturation. The age of animal was reported influences the collagen crosslinks which by that contribute to the gelatin quality. The aims of this study were to determine the characteristics of gelatin that extracted from the skin of adult and sub adult striped catfish (Pangasius hypophthalmus) using acid-base pre-treatment based on pH and FTIR (Fourier Transform Infrared) analysis. The gelatine were extracted from striped catfish skins of adult fish (total body length or TBL = 48.6 ± 1.9 cm) and sub-adult (TBL = 38.9 ± 0.5 cm) using acid-base pre-treatment (CH3COOH and NaOH solution). The pre-treated skin was washed with tap water until neutral. Finally, the swollen skin was mixed with sterile water at a ratio 1/10 (w/v) and extracted using water bath at 40-70 °C for 60 minutes. In general, the pre-treated acid-base of adult and sub adult fish skin resulted the neutral pH gelatin. The FTIR spectra analysis of gelatin from adult and sub adult striped catfish skin exhibited the similar of wavenumbers included amide A, B, I, II, and III region. In both striped catfish skin gelatin adult and sub-adult were shown the functional groups of O-H, C-H, N-H, C = O, C = N, C = C, and N = O.

Enhancing the flotation performance of ilmenite with the magnetic treatment of water

ABSTRACT The flotation water pre-treated in a strong magnetic field was studied to improve the floatability of the ilmenite. Zeta potential measurements, adsorption tests, atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy, and Raman spectroscopy were carried out to reveal the improvement mechanism. After magnetic treatment of the flotation water, the microflotation recovery of ilmenite increased by 5.4% from 74.9% to 80.3%. The adsorption amount of sodium oleate (NaOL) on the ilmenite surface increased and the zeta potential of ilmenite shifted negatively in the presence of NaOL. The analysis of AFM, FTIR, and Raman spectra indicated that magnetic treatment enhanced the hydrogen bonding in the structure of water molecules and a thinner and looser hydration film was formed on the surface of ilmenite, which was contributed to the improvement in the floatability of ilmenite.

Green Synthesis of Zirconia (ZrO2) Nanoparticles using Curcuma Longa Extract and Investigation of Compressive Strength of Epoxy resin (EP)/ZrO2 Nanocomposites

Zirconia (ZrO2) nanoparticles are polymorphic materials having a wide range of applications. It can be synthesized via green as well as chemical synthesis methods. In this work, ZrO2 nanoparticles were synthesized by the green method using Curcuma longa extract. Curcuma longa extract was prepared using the standard method. The synthesized ZrO2 was characterized by the X-ray diffraction (XRD) analysis and Fourier transform infra-red (FTIR) spectroscopy for their structural and size analysis. The analysis of the XRD pattern of ZrO2 showed the tetragonal phase structure and the size was calculated using the Debye Scherrer equation which was about 34.55 nm. The FTIR spectra analysis showed a broad absorption peak particularly at about 774 cm-1 and about 499 cm-1 correspondings to Zr-O2-Zr asymmetric and Zr-O stretching modes, respectively. The characterized ZrO2 nanoparticles were used for the preparation of epoxy resin/ZrO2 nanocomposites. The compressive strength of pure epoxy resin and epoxy resin/ZrO2 nanocomposites were measured by a compressive strength tester and the result indicates the high amount of zirconia was not suitable for the nanocomposites.

ADSORPTION PROPERTIES OF ORGANIC PHOSPHORUS IN WATER BY WATER TREATMENT RESIDUAL

The adsorption kinetics, thermodynamics experiments and adsorption isothermal models of adsorption of adenosine monophosphate(AMP) on aluminum-based water treatment residual (Al-WTR) were studied by batch test. The effects of initial phosphorus concentration, temperature and particle size on the adsorption of AMP were analyzed. The experimental results showed that AMP uptake was favored under neutral and acidic conditions. The smaller the particle size, the larger the adsorption amount. The Al-WTR with size of 1.0~2.0 mm was most suitable for practical application. The pseudo-second-order kinetic model well fitted the adsorption process of Al-AMP on WTR, which indicated that the adsorption was mainly based on chemical adsorption. The Langmuir isotherm model described the adsorption equilibrium well, and the thermodynamic analysis manifested that the adsorption process was a spontaneous, endothermic and entropy-increasing process. The analysis of Fourier-transform infrared spectrum analysis (FTIR) showed that there was a loss of hydroxy groups associated with Al and an increase in Al-O-P bonds during the adsorption of AMP on Al-WTR. This phenomenon was enhanced by the increase of initial phosphorus concentration, indicating that the adsorption of Al-WTR on AMP mainly fulfilled through ligand exchange.

Synthesis and the study of structural, thermal and optical properties of (100-x)Bi2O3-x(BaO-TiO2) glass system

Bismuthbarium titanate glasses have been synthesized by conventional melt quenching technique. X-Ray Diffractograms confirmed the glassy nature of the prepared samples. Various parameters such as density, molar volume, Oxygen packing density (OPD), molar refractivity, molar polarizability, metallization criterion, refractive index and reflection loss have been calculated for understanding the physical and optical characteristics of prepared glasses. A perusal of Differential thermal analysis (DTA) and oxygen packing density (OPD) values confirmed that there is an increase in glass transition temperature (Tg) with increasing BT (BaO-TiO2) content. The analysis of Fourier-transform Infra-Red (FTIR) and Raman spectra clearly confirmed that the glass network mainly comprises of [TiO4], [TiO5] and [TiO6] units which play a significant role as the network formation, network intermediate and network modifier respectively. A non-linear variation of optical band gap energy with the increase in BT (BaO-TiO2) content has been recorded and the same has also been confirmed from the Urbach energy calculations and metallization criterion of the prepared glass samples. The electronegativity and electronic polarizability values revealed the ionic character of the glass samples.

Electromagnetic field of extremely low frequency has an impact on selected chemical components of the honeybee.

The electromagnetic field (EMF) is an environmental factor affecting living organisms. The aim of this study was to demonstrate the effect of an extremely low frequency electromagnetic field (ELF-EMF) on selected chemical components of the honeybee (Apis mellifera L.) using Fourier Transform Infrared (FTIR) spectroscopy. The FTIR method provides information on the chemical structure of compounds through identification and analysis of functional groups. The honeybees were treated with EMF at a frequency of 50 Hz and magnetic induction of 1.6 mT for 2, 6, 12, 24 and 48 hours. Analysis of FTIR spectra showed that EMF exposure longer than 2 hours induced changes in the structure of chemical compounds, especially in the IR region corresponding to DNA, RNA, phospholipids and protein vibrations, compared to control samples (bees not EMF treated). The results confirm the effect of EMF on bees depending on the duration of exposure.

ONE-POT-TWO-STEP SYNTHESIS OF BIOLUBRICANT BASE STOCK FROM RUBBER SEED OIL

Biolubricant base stock was synthesized in this work from rubber seed oil in a one-pot-two-step process of epoxidation and hydroxylation. Rubber seed oil was extracted using a Soxhlet apparatus. The in situ epoxidation of the rubber seed oil with peracid (hydrogen peroxide and acetic acid) was analysed and optimized considering three process variables with their range of values given as temperature of 35-50 , time of 60-180 mins and mole ratio of hydrogen peroxide to acetic acid of 1:0.25-1:1 by applying the central composite design of response surface methodology. The ring opening reaction (hydroxylation) of the epoxide to polyhydroxylated oil (lubricant basestock) with ethanol was carried out using the optimum conditions obtained from the epoxidation process. The rubber seed oil, epoxide and lubricant basestock were characterized in terms of physico-chemical properties using standard methods and in terms of functional groups using Fourier Transform Infrared (FTIR) spectroscopy. Maximum epoxide content of 4.85% and maximum conversion of 71% of rubber seed oil to epoxide was achieved at a temperature of 50􀀀 , reaction time of 180 mins and 1: 0.39 mol/mol of hydrogen peroxide to acetic acid. The predicted values of the epoxidation process reasonably agreed with the experimental ones and model R-squared value of about 95% showed that response surface method can reasonably predict the epoxidation process using a quadratic polynomial model. There was 75% conversion of the epoxide to polyhydroxylated oil (biolubricant basestock), which represents a very high yield. The formation of epoxides and polyhydroxylated oil lead to modification (improvement) in the properties of rubber seed oil as confirmed by the physico-chemical properties and FTIR spectra analysis of the oil, epoxide and lubricant basestock. The study showed that chemical derivatives of rubber seed oils are an attractive, renewable, and ecofriendly alternative to mineral oils for lubricant formulations.

Green Synthesis and Characterization of Copper Nanoparticles and Their Effects on Liver Function and Hematological Parameters in Mice.

The present investigation is based on the green synthesis of copper nanoparticles (CuNPs) from aqueous extract of Capparis spinosa L. fruit. Their effects on liver function and hematological parameters in mice were evaluated. The green synthesis of CuNPs by means of C. spinosa extract was achieved. Ultraviolet-visible spectroscopy, fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy were used to identify the synthesized nanoparticles. BALB/c mice were orally administrated CuNPs at doses of 1000, 2000, and 5000 μg/kg for 2 weeks. Later, the effects of CuNPs on liver function in the treated mice were evaluated by measuring the serum levels of enzymes such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin as well as hematological parameters including hemoglobin, hematocrit, white blood cell, red blood cell, and platelet counts. A maximum peak at wavelength 414 nm confirmed the biosynthesis of CuNPs. FTIR spectrum analysis revealed that the factor groups shaped a coating extract on the surface of the nanoparticles. SEM images demonstrated a particle size between 17 and 41 nm. Although some liver enzymes and hematological parameters increased with increasing dose of extract, there was no significant difference (p>0.05) between oral administrations of CuNPs at doses of 1000, 2000, and 5000 μg/kg and the control group. The findings revealed that CuNPs biosynthesized from aqueous extract of C. spinosa fruit have no toxic effects on the liver functions and hematological parameters of mice. However, more studies are needed for evaluation of the hepatoprotective effects of CuNPs.

The correlation of physicochemical properties of edible vegetable oils by chemometric analysis of spectroscopic data.

This work aimed to investigate and compare the composition and the physicochemical properties of 18 different sources of edible vegetable oils. A systematic study on the correlation between composition and physical properties was performed using Fourier Transform Infrared (FTIR) Spectroscopy and fatty acid chromatographic analysis. Principal component analysis of FTIR spectra is performed to classify edible oils concerning their physical properties. The results demonstrate the potentiality of the method associated with multivariate statistics analysis as powerful, fast, and non-destructive tools for characterization and quality control of edible vegetable oils.

Glyphosate adsorption onto kaolinite and kaolinite-humic acid composites: Experimental and molecular dynamics studies

Glyphosate (PMG) has been the most widely used herbicide in the world, and its environmental mobility and fate are mainly controlled by interactions with mineral surfaces. In soil systems, kaolinite is typically associated with humic acids (HAs) in the form of mineral-HA complexes, and hence it is crucial to characterize the molecular-scale interactions that occur between PMG and kaolinite and kaolinite-HA complexes. Batch experiments, Fourier transform infrared spectrum (FTIR) and X-ray photoelectron spectroscopy (XPS), isothermal titration calorimetry (ITC), and molecular dynamics (MD) simulations were performed to decipher the molecular interactions between PMG and kaolinite and kaolinite-HA composites. Our results reveal that kaolinite-HA composites adsorb higher concentrations of PMG than does kaolinite alone, likely due to more adsorption sites existed on kaolinite-HA than on kaolinite. FTIR and XPS analysis reveal that the carboxyl, phosphonyl and amino groups of PMG interacted with kaolinite and kaolinite-humic acid via Hydrogen bonds. The ITC results and interaction energy calculations indicate that the adsorption of PMG onto the kaolinite-HA is more energetically favorable relative to that onto kaolinite. MD simulations suggest that the PMG molecule adsorbs parallel to the surface of kaolinite and the composites through hydrogen bonding. Humic acid increases the adsorption of PMG through the creation of H-bond networks between PMG, the kaolinite surface, and humic acid. The results from this study improve our molecular-level understanding of the interactions between PMG and two important components of soil systems, and hence yield valuable information for characterizing the fate and behavior of PMG in soil environments.

High-flux neutron irradiation of boron trioxide analyzed with Raman and FTIR spectroscopy

Boron oxide nanopowder is widely used in the production of special glasses and nuclear technology. This paper reports on the optical properties and investigates the formation of new bonds on the structure of nanoboron oxide. In this work, high purity boron oxide samples were irradiated using a energy upto 1 MeV fast neutrons with fluences in the range of [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text] and the neutron flux [Formula: see text] at the room-temperature. Optical properts were determined using Raman spectrum analysis and spectroscopy Fourier transform infrared (FTIR) spectroscopy. FTIR and Raman spectrum analysis were used to explain the chemical bonds and functional groups of nanoboron oxide samples before and after neutron irradiation. When the irradation fluence on [Formula: see text] was increased upto [Formula: see text] it resulted in the formation of a new functional groups [Formula: see text]. From the Raman analysis, the [Formula: see text] was observed to start amorphization after neutron irradiation.

Investigation of spectroscopic and proteomic alterations underlying prostate carcinogenesis

Prostate cancer (PCa) treatment remains challenging, especially in advanced stages, where the lack of sensitivity and specificity of available biomarkers makes it difficult to establish an accurate prognosis. Therefore, it is imperative to study PCa biology to identify key molecules that can improve PCa management. In this study, eight prostate tumor tissues and paired normal tissues were analyzed using two approaches—Fourier-transform infrared (FT-IR) spectroscopy for spectroscopic profiling of biomolecules and antibody microarray for signaling proteins—with the main goal of identifying metabolic and proteomic changes that enable the distinction between normal and tumor conditions. Principal component analysis of FT-IR spectra revealed different spectroscopic signals for each condition. The most relevant changes in prostate tumor tissues identified by FT-IR were dysregulation in lipid metabolism, lower polysaccharide and glycogen content, higher nucleic acid content, and increased protein phosphorylation. Using an antibody microarray, 42 proteins were identified as differentially regulated between the two conditions; 14 of those revealed changes in their phosphorylation status. These proteins include transcription factors and kinases and constitute a highly-interconnected interaction network. Altogether, our data reveal metabolic and proteomic alterations that may be of interest in future translational studies aimed at establishing PCa prognosis and treatment. SignificanceProstate tumor tissues and adjacent benign tissues were analyzed using two approaches—Fourier-transform infrared (FT-IR) spectroscopy for biomolecules and an antibody microarray for signaling proteins, which allowed to identify a panel of metabolic and proteomic alterations that may be of interest in future translational studies to enable the distinction between normal and tumor conditions.

Investigation of hindered phenol antioxidant effects on the aging performance of cross-linked LDPE in the presence of copper

In this study, LDPE materials with different kinds of antioxidants were prepared by melt-blending method. To reveal the aging mechanism and the anti-oxidation efficiency of LDPE in the presence of copper, series of characterizations including tensile testing, Fourier transform infrared spectra (FTIR), differential scanning calorimetry (DSC), scanning electronic microscopy (SEM) and computation simulation were performed. The experimental results indicated that the aging process significantly decreased the tensile strength and elongation of those aged samples except 1024, which retarded the aging degradation of LDPE at the same condition. These results were further confirmed by the FTIR analysis with the carbonyl index values. Additionally, the melting peaks of DSC plots became broader and shifted to the lower temperatures during the aging process for S-0, S-3114 and S-1010, whereas no obvious changes were observed for S-1024. Importantly, according to the results obtained from computation simulation, a strong metal-ligand interaction between hydrazide group and copper ions was formed to prevent the further oxidation, which accounted for the excellent anti-oxidation behavior of 1024 for LDPE in the presence of copper.

Synthesis of mycogenic zinc oxide nanoparticles and preliminary determination of its efficacy as a larvicide against white grubs (Holotrichia sp.)

In the present study, the larvicidal efficacy of mycogenic zinc oxide nanoparticles (ZONPs) were tested against white grubs, a potent pest of sugarcane in western Uttar Pradesh (India). The ZONPs were synthesized using Aspergillus niger biomass and characterized using UV–Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), dynamic light scattering (DLS) and Fourier transform infrared (FTIR). the parts per million (ppm) concentration of synthesized ZONPs was established by the inductively coupled plasma mass spectrometry (ICPMS) technique and several ppm dilutions were prepared to determine 50% lethal dose (LD50). The UV–Vis spectroscopy showed peaks at 240, 290, 340, and 380 nm, corresponding to ZONPs. The FESEM results also confirmed the synthesis of nano-sized particles. EDX analysis result showed the optical absorption peaks specific to ZONPs. The DLS result confirmed the synthesis of ZONPs with sizes ranging from 76.2 to 183.8 nm. The FTIR spectrum analysis confirmed the presence of various functional group interactions in the nanoparticle sample. The ZONPs were tested against the first instar larvae of white grubs. The LD50 was calculated to be 12.63 ppm which still needs to be validated for significance. In the near future, we are planning to establish the minimal lethal dosage of ZONPs to prepare effective larvicidal formulations against white grub infection with minimal toxicity to the environment.