Ten thousands of bone fragments were recovered from the Ma’anshan Paleolithic site, of which 4358 pieces can be identified to skeletal elements or species. In this research, the bone assemblages are quantified based on elements of MNI, MNE, and MAU. Then bone surface modifications and skeletal element profiles of the bone assemblages are studied to understand shlepping behaviors and reveal the technique of disarticulating and defleshing the animals’ bodies, etc. Based on the analyses of bone assemblages and the comparative study with the ethnoarchaeological as well as experimental data, it is assumed that later hominids at the site made fuller use of the game animals than the earlier hominids. Incorporating the lines of evidence from chronological dating, paleoenvironment, and polished bone tools between the two cultural layers, here we propose three hypotheses suggesting that the changes of environment, the migrations of the hominids from the North, or the demographic pulses may cause such a behavioral difference. However, it is yet to be determined which hypothesis can better explain the changes.