This study aimed to evaluate the combined treatment system of domestic sewage and leachate by serial stabilization ponds in a Wastewater Treatment Plant (WWTP). The WWTP had two parallel pond modules, one receiving (module II—pond M10) and the other not receiving leachate (module I—pond M5). Physical, chemical, and biological monitoring parameters collected from January 2017 to December 2021 were evaluated. High reducing efficiency for ammonium nitrogen was found, with an average of 95.7 ± 2.5%. BOD5 and COD can be considered statistically different for a 95% confidence level in the two pond modules. The phytoplankton community was composed of 46 taxa distributed in four taxonomic classes: 6 belonging to the Cyanobacteria group (13%), 22 to the Green Algae group (48%), 4 to the Diatoms group (9%), and 14 to the Phytoflagellate group (30%). No ecotoxicity was detected for the evaluated pond effluent samples. Thus, although the insertion of leachate impacted the quality of the final effluent of the ponds and the organic matter, it may not have affected the effluents’ ecotoxicity. This research helps to understand the impacts of long-term leachate insertion in a real treatment system using stabilization ponds for combined treatment. In addition to the usual efficiency evaluation parameters, ecotoxicological tests and phytoplankton are also evaluated.
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