BackgroundSuperficial wounds that require suturing are often the reason children visit the Paediatric Emergency Department. Suturing is usually accompanied by perilesional administration of lidocaine, a local anaesthetic drug that improves pain tolerance. In paediatric patients, this approach has a low compliance because lidocaine has to be injected, which in children generates fear and anxiety, a sterile anaesthetic gel could improve the child compliance. ObjectiveTo develop a sterile and stable sterile gel capable of remaining in place over time for topical anaesthesia. MethodDifferent formulations were analysed by HPLC, by UV and fluorimetric detection. Two different sterilisation methods were tested. Main outcomeTo maintain the original stability of the gel also after sterilisation process. ResultsFour different gels were prepared and analysed; the most stable gel lasts over 3 months with a degradation less than 10%. ConclusionThe use of Poloxamer 407 guarantees stability of the preparation, showing a reduction in oxidative reaction, and gives the gel the right texture for application to a bleeding wound.