Anaerobically Digested Biosolids Research Articles

Nitrogen release and leachable organic matter decomposition of anaerobically digested biosolids with variable pre-treatments

Thermal hydrolysis pretreatments (THP) coupled to anaerobic digestion (AD) are implemented to treat municipal solids, but limited study indicates whether THP-AD materials merit different land application rates than AD biosolids without THP. Three AD biosolids types with either no pretreatment, THP, or two-hour fermentation were evaluated for differences in leachable dissolved organic matter (DOM), DOM decomposition, and nitrogen (N) releases in biosolids incubated in sandy loam soil. DOM characterizations of size exclusion chromatography, FTIR, and fluorescence maxima>Ex: 400nm indicated similarities in among AD-DOM that contrasted waste activated sludge Milorganite and Suwannee River Organic Matter (SRNOM). Fluorescence peak picking was a more adaptable analysis for shifted leachate spectra than fluorescence regional integration (FRI). Peak ratio analysis is recommended over FRI for biosolids-DOM. The 3-month net inorganic N produced in biosolids-soil incubations was 155±12.1, 149±18.2, 140±17.4, and 354±15.1mgN/kg for AD biosolids with no pretreatment, fermentation-AD, THP-AD, and Milorganite respectively. Overall, there was limited evidence of differences in leachable organic matter quality or net mineral N release after 105days for AD solids with or without THP. Red-shifted fluorescence of leachates decayed by day 45 in aerobic biosolids-soil incubations, suggesting that larger, complex carbon sources liberated during solids stabilization may decompose readily in soils. Overall, other variables (source material, AD operation) may have a greater influence on final nutrient releases and organic matter quality than full-scale pretreatments to AD alone. Critical and targeted application of DOM spectroscopy specific to biosolid-leachates will improve use in advanced stabilization studies.

Biowastes promote essential oil production on degraded soils

Abstract Biowastes (wastes of biological origin) can improve soil fertility but may render the land unsuitable for food production because they introduce contaminants, including heavy metals, pathogens and xenobiotics. We investigated whether sewage waste (pond sludge from Kaikoura and anaerobically-digested biosolids from Christchurch) and Dairy Shed Effluent (DSE) could improve degraded soils for the production of essential oils (EOs). We grew lavender (Lavandula angustifolia Mill.), rosemary (Rosmarinus officinalis L.) and thyme (Thymus vulgaris L.) in two greenhouse experiments in Lismore stony silt loam soil (LSL) amended with pond sludge or biosolids (500–4500 kg N ha−1 equiv.) or DSE (200 kg N ha−1 equiv.). Pond sludge application (2800 kg N ha−1 equiv.) increased the biomass of L. angustifolia and T. vulgaris by 60 % and 62 %, respectively. Christchurch biosolids application up to 1500 kg N ha−1 equiv. to L. angustifolia and R. officinalis increased the biomass of both plant species by up to 86 % and 80 %, respectively. The effect of treatments on EO concentration was insignificant in most cases except for DSE (200 kg N ha−1 equiv.) and Christchurch biosolids at rates >1500 kg N ha−1 equiv., which decreased the EO concentrations in R. officinalis and L. angustifolia. This decrease in EO concentration offset some of the increase in EO production resulting from the increased biomass of the biowaste-amended plants. The ideal EO production increase occurred when Christchurch biosolids were applied at 1500 kg N ha−1 equiv. The benefits of biowaste additions to degraded soils are greater than would occur if they were added to high-fertility soils. Heavy metal concentrations in all treatments were below food safety standards. Biowastes could rebuild degraded soils and produce valuable EOs, thereby reducing the economic and environmental costs of biowaste disposal, while improving soil fertility and generating revenue from otherwise underproductive land.

Impact of anaerobically digested biosolids characteristics and handling conditions on dewatering performance at multiple facilities.

Anaerobically digested biosolids (ABD) characteristics that affect dewatering were assessed at three water resource recovery facilities (WRRF) with different handling practices. Dewatering performance at the three sites corresponded to different levels ofsoluble chemical oxygen demand (COD), ammonia (NH4 -N), and mono- and divalent cation concentrationsin ADB. Capillary suction time (CST) and a modified centrifugal technique were used to determine optimum polymer doses and to assess the impact of handling conditions on dewatering performance. Both techniques indicated that polymer dosing between 15 and 20kg/dry tonne was optimal for all facilities and that biosolids mixing and pumping did not significantly impact dewaterability. The CST values of anaerobically digested biosolids decreased as temperature increased, but no significant difference was found for either temperature or location of dewatering facilities. Sludge viscosity and rheological properties that vary with temperature appeared to have influenced CST values. Modified centrifugal technique results indicated cake solids were not affected by polymer make-up water or ADB temperature when emulsion polymer was used. This study shows the value of laboratory testing of biosolids under controlled conditions to identify and correct potential problems in full-scale operations. PRACTITIONER POINTS: Capillary suction time and a modified centrifugal technique were used to assess the impact of different process-related and environmental factors on dewatering. Higher concentrations of soluble COD (potentiallyextracellular polymeric substances - EPS) and low calcium (Ca) in anaerobically digested biosolids align with reduced dewaterability. Cell disruption and break down of floc structures due to storage/mixing and pumping of biosolids did not appear to negatively impact dewatering. Modified centrifugal test results did not provide conclusive evidence of whether dewatering of anaerobically digested biosolids could be significantly impacted by temperature over the range 15-30°C, especially when emulsion polymer is used. This study shows the value of laboratory testing of biosolids under controlled conditions to identify potential problems in the full-scale operations.

Dissipation and persistence of sulfonamides, quinolones and tetracyclines in anaerobically digested biosolids and compost during short-term storage under natural conditions

This study investigated the dissipation and persistence of three groups of residual antibiotics (sulfonamides, quinolones, and tetracyclines) in anaerobically digested (AD) biosolids and compost during 28 days of storage under environmental conditions. Results showed that the total dissipation of sulfonamides was above 70%, which was higher than that of quinolones and tetracyclines. Quinolones were more persistent in compost than in AD biosolids. Similar dissipation rates in AD biosolids and compost were observed for tetracyclines. Of the four commonly used models, the availability-adjusted first-order model (AAFO) was the optimal to fit the dissipation of antibiotics, which was mainly governed by their initial concentrations, matrix pH, and the presence of organic matter and microorganisms. The half-lives of sulfonamides, quinolones, and tetracyclines in AD biosolids were 6-51 days, 1-136 days, and 15-19 days; while those were 3-21 days, 3-74 days, and 7-27 days in compost, respectively. In particular, enrofloxacin and ofloxacin were the most persistent in AD biosolids and compost, respectively. Moreover, tetracyclines were more prone to cause pseudo-persistent pollution due to their much higher residuals in comparison to sulfonamides and quinolones. Thus, both AD biosolids and compost should be further treated before their farmland applications to control antibiotic introduction to the environment.

Impact of pre-application treatment on municipal sludge composition, soil dynamics of antibiotic resistance genes, and abundance of antibiotic-resistance genes on vegetables at harvest

In many jurisdictions sludge recovered from the sewage treatment process is a valued fertilizer for crop production. Pre-treatment of sewage sludge prior to land application offers the potential to abate enteric microorganisms that carry genes conferring resistance to antibiotics. Pre-treatment practices that accomplish this should have the desirable effect of reducing the risk of contamination of crops or adjacent water with antibiotic resistance genes carried in these materials. In the present study, we obtained municipal sludge that had been subjected to one of five treatments. There were, anaerobic-digestion or aerobic-digestion, in both instances with and without dewatering; and heat-treatment and pelletization. Each of the five types of biosolids was applied to an agricultural field at commercial rates, following which lettuce, carrots and radishes were planted. Based on qPCR, the estimated antibiotic gene loading rates were comparable with each of the five biosolids. However, the gene abundance in soil following application of the pelletized biosolids was anomalously lower than expected. Following application, the abundance of antibiotic resistance genes decreased in a generally coherent fashion, except sul1 which increased in abundance during the growing season in the soil fertilized with pelletized biosolids. Based on qPCR and high throughput sequencing evidence for transfer of antibiotic resistance genes from the biosolids to the vegetables at harvest was weak. Clostridia were more abundant in soils receiving any of the biosolids except the pelletized. Overall, the behavior of antibiotic resistance genes in soils receiving aerobically or anaerobically-digested biosolids was consistent and coherent with previous studies. However, dynamics of antibiotic resistance genes in soils receiving the heat treated pelletized biosolids were very different, and the underlying mechanisms merit investigation.

Increasing thermal drying temperature of biosolids reduced nitrogen mineralisation and soil N2O emissions.

Previous studies found that thermally dried biosolids contained more mineralisable organic nitrogen (N) than the raw or anaerobically digested (AD) biosolids they were derived from. However, the effect of thermal drying temperature on biosolid N availability is not well understood. This will be of importance for the value of the biosolids when used to fertilise crops. We sourced AD biosolids from a Danish waste water treatment plant (WWTP) and dried it in the laboratory at 70, 130, 190 or 250°C to >95% dry matter content. Also, we sourced biosolids from the WWTP dried using its in-house thermal drying process (input temperature 95°C, thermal fluid circuit temperature 200°C, 95% dry matter content). The drying process reduced the ammonium content of the biosolids and reduced it further at higher drying temperatures. These findings were attributed to ammonia volatilisation. The percentage of mineralisable organic N fraction (min-N) in the biosolids, and nitrous oxide (N2O) and carbon dioxide (CO2) production were analysed 120days after addition to soil. When incubated at soil field capacity (pF 2), none of the dried biosolids had a greater min-N than the AD biosolids (46.4%). Min-N was lowest in biosolids dried at higher temperatures (e.g. 19.3% at 250°C vs 35.4% at 70°C). Considering only the dried biosolids, min-N was greater in WWTP-dried biosolids (50.5%) than all of the laboratory-dried biosolids with the exception of the 70°C-dried biosolids. Biosolid carbon mineralisation (CO2 release) and N2O production was also the lowest in treatments of the highest drying temperature, suggesting that this material was more recalcitrant. Overall, thermal drying temperature had a significant influence on N availability from the AD biosolids, but drying did not improve the N availability of these biosolids in any case.

Metal concentrations in lime stabilised, thermally dried and anaerobically digested sewage sludges

Cognisant of the negative debate and public sentiment about the land application of treated sewage sludges ('biosolids'), it is important to characterise such wastes beyond current regulated parameters. Concerns may be warranted, as many priority metal pollutants may be present in biosolids. This study represents the first time that extensive use was made of a handheld X-ray fluorescence (XRF) analyser to characterise metals in sludges, having undergone treatment by thermal drying, lime stabilisation, or anaerobic digestion, in 16 wastewater treatment plants (WWTPs) in Ireland. The concentrations of metals, expressed as mgkg(-1) dry solids (DS), which are currently regulated in the European Union, ranged from 11 (cadmium, anaerobically digested (AD) biosolids) to 1273mgkg(-1) (zinc, AD biosolids), and with the exception of lead in one WWTP (which had a concentration of 3696mgkg(-1)), all metals were within EU regulatory limits. Two potentially hazardous metals, antimony (Sb) and tin (Sn), for which no legislation currently exists, were much higher than their baseline concentrations in soils (17-20mgSbkg(-1) and 23-55mgSnkg(-1)), meaning that potentially large amounts of these elements may be applied to the soil without regulation. This study recommends that the regulations governing the values for metal concentrations in sludges for reuse in agriculture are extended to include Sb and Sn.

Application of biosolids to soil affects Cu and Zn accumulation and antioxidant activity of lettuce (Lactuca sativa L.)

abstractThe application of biosolids to agricultural fields is becoming increasingly common. The effect of biosolids on the behavior of metals in different plants has been reported to be variable and ambiguous. A greenhouse experiment was conducted on a soil spiked with four rates of copper (Cu) (0, 50, 100, and 150 mg kg−1) and zinc (Zn) (0, 150, 300, and 450 mg kg−1) in lettuce culture. When Cu and Zn were spiked to anaerobically-digested biosolids, their availability in lettuce increased. Root and shoot fresh weight decreased due to a decrease in photosynthetic rate. superoxide dismutase (SOD) and peroxidases (POD) activity increased after the application of Cu and Zn. The bioavailability of Zn in lettuce was greater than that of Cu because of a higher transfer factor in plants and due to a higher endogenous Zn concentration.These results will allow for better fertilization management when biosolids are applied to tomato culture.

N Mineralisation from Bioresources Incubated at 12.5°C

Soils treated with lime-amended biosolids (LAB), poppy seed waste (PSW), anaerobically digested biosolids (ADB) and poppy mulch (PM) and incubated at 12.5°C for 56 days released 45%, 36%, 25%, and −8%, respectively, of total applied N as plant available nitrogen (PAN) by the end of the incubation. The mineralisation rates were contrary to expectations based on the C : N ratios of the four products: LAB (5 : 1), PSW (7 : 1), ADB (3 : 1), and PM (16 : 1). PM showed a significant negative priming effect over the incubation period. These results have implications for production agriculture in temperate regions where application and incorporation of bio-resources traditionally occurs in autumn and spring when soil and air temperatures are relatively low. Current application times may not be suitable for nitrogen release to satisfy crop demand.

Leaching potential and forms of phosphorus in deep row applied biosolids underlying hybrid poplar

Abstract Biosolids deep row incorporation (DRI) offers an alternative recycling approach for bioenergy crop production, but phosphorus additions in excess of the vegetation requirements can potentially impair water quality. The effects of DRI of anaerobically digested (AD) and lime-stabilized (LS) biosolids at four rates (213, 426 Mg ha −1 for AD and 328, 656 Mg ha −1 for LS) and a single rate of conventional P fertilizer on leaching of PO 4 –P, and TKP were investigated in a hybrid poplar plantation in a coastal plain heavy mineral mine reclamation site. The effects of AD and LS biosolids aging on the transformations and losses of P applied with the biosolids were also studied. Zero tension lysimeters were installed at the site to collect leachate. Samples were collected at least monthly for 30 months between July 2006 and December 2008. We also analyzed biosolids P (labile P, Al-, Fe-bound-P, Ca-bound-P, total P, Mehlich 1 P) fractions. The loss of P was determined and P binding constituents were described via regression analysis. Orthophosphate and TKP from the DRI biosolids leached in negligible amounts, and were similar to leaching from conventional P fertilization. Most of the P was Al- and Fe-bound in the AD biosolids and Ca-bound in the LS biosolids. Labile P decreased with decrease in organic C, which indicated that P leaching potential from the DRI biosolids decreased with aging. Our results indicated that P mobility from biosolids of the type used should not pose a water quality risk.

Transformations of Nitrogen and Carbon in Entrenched Biosolids at a Reclaimed Mineral Sands Mining Site

Biosolids deep-row incorporation (DRI) provides high levels of nutrients to the reclamation sites; however, additions of N in excess of the vegetation requirements can potentially impair water quality. The effects of anaerobically digested (AD) and lime stabilized (LS) DRI biosolids and inorganic N fertilizer were compared on C and N transformations and transport at a reclaimed mineral sands mining site. Biosolids were applied at 213 and 426 Mg AD biosolids ha(-1) and 328 and 656 Mg LS biosolids ha)(-1) (dry mass), and inorganic N fertilizer was applied at 0 (control) and 504 kg N ha-(-1) yr(-1). Zero tension lysimeters were installed to collect leachate for determination of vertical N transport, and the biosolids seams were analyzed for N and C transformations after 28 mo aging. The leachijng masses from the DRI biosolids treatments were 139 to 291 kg ha(-1) NO3-N, 61 to 243 kg ha(-1) NH4-N, and 61 to 269 kg ha(-1) organic N, while the fertilizer treatment did not differ from the control. Aged biosolids analysis showed that total N lost over the course of 2 yr was 15.2 Mg ha(-1) and 10.9 Mg ha(-1) for LS and AD biosolids, respectively, which was roughly 50% of the N applied. Organic C losses were 81 Mg ha(-1) and 33 Mg ha(-1) for LS and AD biosolids, respectively. Our results indicated that entrenchment of biosolids in coarse-textured media should not be used as a mined land reclamation technique because the anaerobic conditions required to limit mineralization and nitrification cannot be maintained in such permeable soils.

Plant growth and soil responses to soil applied organic materials in Tasmania, Australia

Biosolids, poppy mulch (PM), and poppy seed waste (PSW) were applied to soils in barley and wheat field trials in two areas of Tasmania to determine crop and soil responses and the potential for these materials to substitute for inorganic fertiliser. Lime-amended biosolids (LAB) was applied at one, two, and five times the nitrogen-limiting biosolids application rate (NLBAR). Anaerobically digested biosolids (ADB) was applied at the NLBAR, and PM and PSW were applied at industry-recommended rates. The results indicated that ADB, LAB, PM, and PSW can substitute for inorganic fertiliser in meeting plant nutrient requirements but may be releasing more plant-available N than guideline assumptions. However, organic amendments are less easily managed and applied than inorganic fertiliser, and release of plant-available N from organic amendments may be too rapid for plant uptake to prevent leaching losses. LAB can also leave elevated residual extractable phosphorus in soil after two cereal crops, and PM and LAB can increase soil pH. Microbial biomass showed an inconsistent, and in some cases unexpected, response to organic amendments after 1 year and no significant relationship to changes in soil carbon.

Effect of Long-Term Application of Biosolids on Molybdenum Content and Quality of Winter Wheat Forage

Forages that accumulate excessive molybdenum (Mo) from excessive land application of Mo can cause molybdenosis, a copper (Cu) induced deficiency in ruminants. Limited information is available on the effect of land-applied biosolids, Mo content, and quality of winter wheat forage (Triticum aestivum L.). The objectives of this study were (1) to determine the effect of biosolids application on tissue molybdenum and tissue copper (Cu):Mo of winter wheat forage and, (2) to measure the Mo uptake coefficient value (UC) for winter wheat under field conditions and compare it with the U.S. EPA Part 503 risk-based UC value of 0.42. Two nitrogen sources, anaerobically digested biosolids and ammonium nitrate, were applied annually from 1993–2001 to continuous winter wheat. The experimental design was a complete factorial arrangement of treatments composed of six nitrogen (N) rates (0, 45, 90, 180, 269, and 539 kg N ha−1 yr−1) and two N sources (anaerobically-digested biosolids and ammonium nitrate, 34-0-0). Application of biosolids did not significantly alter soil pH, which ranged from 6.6 to 7.2. Biosolids application increased soil Cu from 7.19 to 19.6 mg kg-1 and soil Mo from 0.44 to 1.02 mg/kg. Forage uptake of Mo and Cu showed temporal variation between years but increased with biosolids application rate. The Cu:Mo ratio of washed forage was > 2.9 and was inversely related to biosolids application rate. Forage Mo was < 2 mg kg-1. Unwashed forage had a larger Cu:Mo ratio than washed forage. Forage Mo increased with biosolids application for unwashed forage. However, the Cu:Mo ratio of unwashed forage decreased with increased biosolids application and the unwashed forage Cu:Mo ratio was > 10. Biosolids application had no effect on forage sulfur (S) content that was < 3000 mg kg-1. The calculated UC of Mo for winter wheat in Oklahoma of 0.24 (washed forage) and 0.36 (unwashed forage) is less than the UC of 0.42 used by U.S. EPA in its risk assessment governing land application of biosolids. These results suggest winter wheat forage produced using biosolids presents minimal risk of molybdenosis to livestock. Ingestion of soil on unwashed forage increases forage Cu:Mo and offers more protection from the risk of molybdenosis.