Anaerobic Treatment Research Articles

Impact of Anaerobic Soil Disinfestation Treatment on the Properties of Tomato Continuous Cropping Soil

Impact of Anaerobic Soil Disinfestation Treatment on the Properties of Tomato Continuous Cropping Soil

Performance evaluation of ICX reactor in treatment of paper mill wastewater: a case study in South Vietnam.

This study evaluates the performance of the Internal Circulation eXperience (ICX) reactor in treating high-strength paper mill wastewater in the south of Vietnam. The ICX reactor effectively managed organic concentrations (sCOD) of up to 11,800 mg/L. Results indicate a volumetric loading rate (VLR) of 26.8 kg/m3 × day, achieving processing efficiency exceeding 81% while consistently maintaining volatile fatty acids (VFA) below 300 mg/L. The study employed Monod and Stover-Kincannon kinetic modeling, revealing dynamic parameters including Ks = 56.81 kg/m3, Y = 0.121 kgVSS/kgsCOD, Kd = 0.0242 1/day, μmax = 0.372 1/day, Umax = 151 kg/m3 × day, and KB = 175.92 kg/m3 × day, underscoring the ICX reactor's superior efficiency compared to alternative technologies. Notably, the reactor's heightened sensitivity to VFA levels necessitates influent concentrations below 1,400 mg/L for effective sludge treatment. Furthermore, the influence of calcium on treatment efficiency requires post-treatment alkalinity maintenance below 19 meq/L to stabilize MLVSS/MLSS concentration. Biogas production ranged from 0.6 to 0.7 Nm3 biogas/kg sCOD; however, calcium impact diminished this ratio, reducing overall treatment efficiency and biogas production. The study contributes valuable insights into anaerobic treatment processes for complex industrial wastewaters, emphasizing the significance of controlling VFA, calcium, and alkalinity for optimal system performance.

Full-scale study on high-rate low-temperature anaerobic digestion of agro-food wastewater: process performances and microbial community.

The fast-growing global population has led to a substantial increase in food production, which generates large volumes of wastewater during the process. Despite most industrial wastewater being discharged at lower ambient temperatures (<20 °C), majority of the high-rate anaerobic reactors are operated at mesophilic temperatures (>30 °C). High-rate low-temperature anaerobic digestion (LtAD) has proven successful in treating industrial wastewater both at laboratory and pilot scales, boasting efficient organic removal and biogas production. In this study, we demonstrated the feasibility of two full-scale high-rate LtAD bioreactors treating meat processing and dairy wastewater, and the microbial communities in both reactors were examined. Both reactors exhibited rapid start-up, achieving considerable chemical oxygen demand (COD) removal efficiencies (total COD removal >80%) and generating high-quality biogas (CH4% in biogas >75%). Long-term operations (6-12 months) underscored the robustness of LtAD bioreactors even during winter periods (average temperature <12 °C), as evidenced by sustained high COD removal rates (total COD removal >80%). The stable performance was underpinned by a resilient microbial community comprising active acetoclastic methanogens, hydrolytic, and fermentative bacteria. These findings underscore the feasibility of high-rate low-temperature anaerobic wastewater treatment, offering promising solutions to the zero-emission wastewater treatment challenge.

Enhanced methane production from anaerobic wastewater treatment by carbon nanotubes: Surface defect mediated interfacial electron transfer

Interfacial electron transfer, a crucial process in direct interspecies electron transfer (DIET) between exo-electrogenic microorganisms and conductive materials, has played a vital role in enhancing methane production in anaerobic wastewater treatment. To improve interfacial electron transfer, the construction of surface defect has been identified as an effective approach. In this study, a range of metals and non-metals were used to dope carbon nanotubes (CNTs), which were served as the conductive materials and added into anaerobic wastewater treatment systems. The effect of surface defect of CNTs on methane production were investigated. The results demonstrated that all the doped CNTs can significantly increase methane production rate in anaerobic systems. Among them, boron and iron doped CNTs (B-CNTs and Fe-CNTs) exhibited the most promising performance as non-metal and metal doped CNTs. The addition of B-CNTs and Fe-CNTs led to impressive increase of 101.6% and 149.8% in the methane production rate of the anaerobic systems. Furthermore, this study elucidated the underlying mechanisms responsible for the enhanced methane production in the anaerobic wastewater treatment with doped CNTs. The introduction of doped CNTs enriched exo-electrogenic bacteria and archaea, thereby promoting the DIET process. Additionally, the CNTs also acted as the capacitor, storing and discharging electrons, which contributed to the maintenance of a stable methane production in anaerobic wastewater treatment.

Synergizing sugarcane waste valorization: Optimization of two-stage anaerobic co-digestion for enhanced methane recovery and organic matter removal

Multiple strategies have been used in anaerobic digestion of effluents aiming to enhance the efficiency of the methane (CH4) recovery process, where co-digestion and the two-stage process emerge as prominent methods. In this context, this study aimed at evaluating the co-digestion of sugarcane vinasse and molasse in a two-stage process using anaerobic fluidized bed reactors (AFBR), verifying the effect of increasing the organic loading rate (OLR) (5–22.5 kg COD.m−3.d−1) and temperature in the second stage methanogenic reactor. Regarding the two-stage process, sugarcane vinasse and molasses fermentation demonstrated the maximum energy potential (E.P) of 57.08 kJ.d−1 (7.5 g COD.L−1). Regarding the methanogenic sequential reactors, organic matter removal reached high values (up to 84.5 % in a thermophilic sequential reactor (TSR); up to 84.5 % in a mesophilic sequential reactor (MSR)). The highest CH4 yield (MY) of 306.77 ± 38.84 mL CH4.g−1 COD rem was observed in MSR (9 kg COD.m−3.d−1). Meanwhile, the maximum value of MPR (3.8 ± 0.5 L CH4.L−1.d−1) was observed in TSR (21 kg COD.m−3.d−1). Regarding the Archaea domain, a predominance of hydrogenotrophic microorganisms could be identified, in which the genera Methanobacterium and Methanothermobacter were the most abundant in MSR and TSR, respectively. This study demonstrates that anaerobic digestion of sugarcane vinasse and molasses enhances anaerobic treatment using two-stage AFBRs. It not only produces CH4 but also generates hydrogen and high-value products through an acidogenic reactor. This research marks a significant advancement in managing sugarcane processing by-products, offering promising approaches to maximize biogas production and improve pollutant removal efficiency.

Using the kriging method based on cross-validation stepwise variable selection to optimize anaerobic wastewater treatment process

In this study, process parameters for wastewater treatment are optimised in order to improve the efficiency of energy recovery from wastewater treatment. In order to identify process parameters that have a significant impact on carbon recovery, we propose a stepwise forward variable selection method based on K-fold cross kriging to validate the loss ER.The results show that for complex wastewater treatment systems, the cross-validated kriging-based stepwise variable selection method can predict carbon recovery quickly and accurately. Finally, this paper combines the kriging prediction model with the PSO optimisation strategy to determine the optimal set points of process parameters with the objective of maximising carbon recovery.

Investigation of organic content and microbial characteristics distribution at the pretreatment units for wastewater treatment plant sustainable upgrading

It is necessary and significant to acquire the influent characteristics in the whole pretreatment units of the activated sludge process in wastewater treatment plants (WWTPs) for the sustainable upgrading. In this work, we drawn the samples and continuously investigated all the structure units of the pretreatment section in a typical wastewater treatment plant including the anaerobic and aerobiotic treatment processes. We monitored the routine environmental parameters in the water, including dissolved oxygen (DO), pH, and suspended solids (SS). At the same time, we investigated the concentration distribution patterns of chemical oxygen demand (COD) and biochemical oxygen demand (BOD5) and found that the degradation of organic matter in the sewage continued in the pretreatment section. And the remarkable decreasing tendency of organic pollution concentration was confirmed along the flow direction in the pretreatment section, suggesting a modest depletion effect on influent organic matter of the pretreatment process. Moreover, to further validate the degradation process of organic compounds in the preprocessing procedure, analysis and detection were conducted on the types of organic compounds and the microbial community structures in the water samples collected at various sampling points through three-dimensional fluorescent spectroscopy and high-throughput sequencing, respectively. Conclusively, the detailed and accurate influent characteristics can support the scientific basis for the WWTP sustainable operation, management, and upgrading, especially for that with the low C/N ratio influent.

The important role of high sludge concentration in anaerobic biological systems with low COD/SO42- sulfate-containing wastewater predicted by machine learning: Insights from microbial community and metabolic pathways

In anaerobic biological systems, sulfate reduction efficiency is influenced by multiple factors synergistically, particularly under low chemical oxygen demand-to-sulfate ratio (COD/SO42-) conditions. Enhancing sulfate reduction efficiency under low COD/SO42- conditions is challenging due to the deficiency of electron donors. In this study, two anaerobic systems were operated with a gradual decrease in COD/SO42-. The primary objective was to investigate the correlations among various influential factors, the efficacy of sulfate reduction, and the progression of microbial communities. The results showed that even with a decrease in COD/SO42- to 0.1, the sulfate reduction efficiency could reach 70% under high sludge concentration. Machine learning predictions identified sludge concentration as the most influential factor under low COD/SO42- conditions. The functional gene analysis confirmed that the sulfate reduction under high sludge concentration was primarily governed by the dissimilatory pathway and exhibited minimal reliance on COD/SO42-. These findings provide theoretical support and a novel approach for the anaerobic biological treatment of sulfate-containing wastewater under low COD/SO42- conditions.

Anaerobic co-digestion of food waste and excess sludge by humus composites: Characteristics and microbial community structure

Volatile fatty acids (VFA) produced during the anaerobic co-digestion (Co-AD) an Manual removal of impurities such as bones, fish spines, napkins, etc., after collection at the food waste collection sited hydrolysis of food waste (FW) and excess sludge (ES) are important factors affecting the stability of the digestion and biogas production. This study aimed to use humus composites (HS) to mediate the Co-AD system of FW and ES, investigate their effects on VFA conversion, and analyze the microbial community structure using high-throughput sequencing technology to reveal the mechanism of action of HS in mediating the Co-AD system from a microbial perspective. The amount of HS was positively correlated with the accumulation of VFA produced by the hydrolysis of co-digested substrates, and in the Co-AD system with 5.00 g/L HS, the accumulation of VFA increased by approximately 1.1 times, whereas the pH and oxidation reduction potential of the system tended to be in the benign range, offering a favoring condition for enhancing the stability of the system. The biogas production increased by 46.3 % compared with that of the blank group. HS significantly increased the relative abundance values of Synergistota, a mutualistic symbiont with acetic acid-producing bacteria, and Proteobacteria, a mutualistic symbiont with methanogenic bacteria, thus promoting VFA conversion and strengthening the Co-AD efficacy of FW and ES. This study provides a simple and effective method to improve the Co-AD efficacy of FW and ES, as well as a new approach for the anaerobic resource treatment of other organic solid wastes.

Scale-up of plug-flow reactors in anaerobic treatment of agro-industrial wastes

This study is aiming to evaluate the optimization potential of anaerobic digestion systems for the treatment of complex agro-industrial wastes at an industrial scale. In previous work, the performance of a 20 L pilot-scale Plug Flow Reactor (PFR) that was able to operate at Organic Loading Rates (OLRs) of up to 25 kg Chemical Oxygen Demand (COD) m−3 d−1, was demonstrated. This concept was then successfully transferred in a semi-industrial scale PFR of a volume equal to 50 m3, and the optimal operational parameters were evaluated. The construction of a full industrial scale facility followed, utilizing initially a 380 m3 PFR. Since PFR systems in practice do not behave ideally, due to short Length to Diameter ratio (L/D) and/or higher axial dispersion, the ideal PFR behavior was compared with real data of the non-ideal industrial-scale system; a performance reduction of 25–30 % was detected. However, the disadvantage of the non-ideal behavior of PFRs can be overcome by the cascaded arrangement of two such reactors (2 × 380 m3 PFRs), leading to a total volume reduction of 35 %, as depicted by experimentation on the industrial scale cascaded PFRs. The optimal design parameters for the PFRs are provided.

Synergetic effect of biochar and intermittent electricity stimulation on mitigating the adverse effects of tannic acid on anaerobic granular sludge in wastewater

Tannic acid (TA), a common industrial contaminant in sectors such as papermaking and leather production, affects the anaerobic treatment of wastewater by forming complexes with proteins in sludge granules, thereby inhibiting microbial efficiency and promoting sludge deflocculation. Although techniques such as intermittent electricity can promote microbial growth, they do not effectively resolve sludge deflocculation. Additionally, although biochar (BC) enhances the abundance of microbes, it does not significantly improve microbial activity or treatment performance. This study aimed to alleviate the adverse effects of TA on anaerobic granular sludge (AnGS) by synergistically applying intermittent electrical stimulation and BC. Co-treatment with 1 h-on/off intermittent electricity and 3 g/L BC notably reduced granular sludge deflocculation. Consequently, the chemical oxygen demand (COD) removal efficiency in wastewater reached 91.73 %, with a maximum methane production per cycle of 230.59 mL/g COD, 16.95 % higher than that of the control reactor (197.17 mL/g COD). This improvement was attributed to the synergistic action of intermittent electricity and BC, which enriched the functional microorganisms and stimulated their activity, thereby facilitating direct interspecies electron transfer. Additionally, enhancing the polysaccharide and protein levels in extracellular polymeric substances improved the stability of granular sludge, ultimately mitigating the adverse effects of TA on AnGS.

Magnetite encapsulated in carbon shell particles (Fe3O4@C) to boost anaerobic methanogenesis of chloramphenicol wastewater

Magnetite (Fe3O4) is extensively applied to enhance efficacy of anaerobic biological treatment systems designed for refractory wastewater. However, the interaction between magnetite, organic pollutants and microorganisms in digestion solution is constrained by magnetic attraction. To overcome this limitation and prevent magnetite aggregation, the core-shell composite materials with carbon outer layer enveloping magnetite core particles (Fe3O4@C) were developed. The impact of Fe3O4@C with varying Fe3O4 mass ratios on the anaerobic methanogenesis capability in the treatment of chloramphenicol (CAP) wastewater was investigated. Experimental results demonstrated that Fe3O4@C not only enhanced chemical oxygen demand (COD) removal efficiency and biogas production by 2.42-13.18% and by 7.53%-23.25%, respectively, but also reduced the inhibition of microbial activity caused by toxic substances and the secretion of extracellular polymeric substances (EPS) by microorganisms responding to adverse environments. The reinforcing capability of Fe3O4@C increased with the rise in Fe3O4 content. Furthermore, High-throughput pyrosequencing illustrated that Fe3O4@C enhanced the relative abundance of Methanobacterium, a hydrogen-utilizing methanogen capable of participating in direct interspecies electron transfer (DIET), by 5%. Metagenomic analysis indicated that Fe3O4@C improved the decomposition of complex organics into simpler compounds by elevating functional genes encoding key enzymes associated with organic matter metabolism, acetogenesis, and hydrogenophilic methanogenesis pathways. These findings suggest that Fe3O4@C have the potential to strengthen both the hydrogenophilic methanogenesis and DIET processes. This insight offers a novel perspective on the anaerobic bioaugmentation of high-concentration refractory organic wastewater.

Effects of anaerobic treatment on the non-volatile components and angiotensin-converting enzyme (ACE) inhibitory activity of purple-colored leaf tea

This study investigated the effect of anaerobic treatment on the non-volatile components and angiotensin-converting enzyme (ACE) inhibitory activity in purple-colored leaf tea. Results showed that after 8 h of anaerobic treatment, the γ-aminobutyric acid (GABA) content significantly increased from 0.02 mg/g to 1.72 mg/g (p < 0.05), while lactic acid content gradually rose from non-detectable levels to 3.56 mg/g. Notably, certain flavonols like quercetin and myricetin exhibited significant increments, whereas the total anthocyanins (1.01 mg/g) and epigallocatechin-3-(3''-O-methyl) gallate (13.47 mg/g) contents remained almost unchanged. Furthermore, the ACE inhibition rate of purple-colored leaf tea increased significantly from 42.16% to 49.20% (p < 0.05) at a concentration of 2 mg/mL. Moreover, galloylated catechins showed stronger ACE inhibitory activity than non-galloylated catechins in both in vitro ACE inhibitory activity and molecular docking analysis. These findings might contribute to the development of special purple-colored leaf tea products with potential therapy for hypertension.

Adsorption enhanced biological treatment of hydrothermal liquefaction aqueous phase derived from municipal sludge

Hydrothermal liquefaction (HTL) is a promising method for municipal sludge valorization through waste minimization and biofuel production. The process wastewater, HTL aqueous, presents a significant challenge for scale-up due to recalcitrant compounds. In this study, granular activated carbon (GAC) was used to remove potential inhibitors from HTL aqueous through adsorption to enhance aerobic and anaerobic biological treatment. GAC removed up to 61 % chemical oxygen demand (COD), 50 % biochemical oxygen demand (BOD) and potential inhibitors, such as total phenolic compounds (87 %) and N-heterocycles (90 % of pyridines) at 100 g/L. Conversely, most volatile fatty acids remained in HTL aqueous. Subsequently, mesophilic and thermophilic specific methane potential increased by up to 97 % and 83 %, respectively. BOD increased by up to 50 %, which enhanced BOD/COD ratio from 81 % to 93 % before and after adsorption. This study established the groundwork for HTL aqueous adsorption, described mechanism for pollutant removal, and provided insights for biological treatment.

Ozonation process at neutral pH integrated with anaerobic treatment system to methylparaben removal

The incomplete removal of emerging pollutants, such as parabens, from wastewater effluents represents a major pathway for their release into the environment. The development of advanced technologies integrated into well-known conventional systems is a solution. Advanced oxidative processes (AOP), such as ozonation and its combinations, are technologies based on the generation of hydroxyl radicals (HO•) capable of removing these pollutants, representing an alternative solution to this problem. Therefore, the present study aimed to investigate the ozonation process combined with hydrogen peroxide (O3/H2O2) in the treatment of anaerobic effluent post-treated by a real-scale upflow anaerobic sludge blanket (UASB) reactor, enriched with methylparaben (MeP). The study was conducted under optimized conditions and at neutral pH of the effluent. To investigate the influence of variables, as ozone concentration, hydrogen peroxide concentration, pH and time, a 24 Factorial experimental design followed by a 22 Doehlert optimization design was implemented. The optimal degradation occurred with 25.0 mg L−1 of O3 and 30.0 mg L−1 of H2O2 at neutral effluent pH, achieving a removal efficiency of 71.6 % after 60 min and 85.5 % after 120 min. The optimized O3/H2O2 process after 60 min achieved a 95.0 % removal of organic matter by biochemical oxygen demand (BOD), reducing the initial concentration from 138 mg L−1 to 7 mg L−1. Acute toxicity test with Artemia salina and Lactuca sativa, indicated an absence of toxicity (TU < 0.4). The findings of this study indicate the positive efficiency of combining post-UASB effluent treatment with O3/H2O2 processes for the removal of methylparaben.

Anaerobic Treatment of N-(Phosphonometyl) Glycine Using Mixed Culture in Batch Reactor

Pesticides have been used in the fight against various pests to increase crop growth in our country and the world. In recent years, high concentrations of toxic and detection of carcinogenic pesticides indicates that these substances are transported to groundwater and pollute water resources. N- (phosphonomethyl) glycine are also used in large quantities. In this study, the optimization of anaerobic treatment of N- (phosphonomethyl) glycine was investigated by applying a statistical-based experimental design. Full factorial trials different initial pesticide concentrations and cosubstrate type were established, making up 9 different experimental setups. Trials were performed in 2 replicates. The experiments were carried out in Oxitop C bottles with stirring at a working volume of 200 mL. The pH was adjusted to 7 ± 0.2. Trials were performed at 35oC for 30 days. At the end of the process, the removal of the trials according to the input and output COD and pesticide values were examined. As a result, the most efficient COD removal was achieved 98,96 % when pesticide concentration of 5 mg L-1 and glucose as cosubstrate were used. The highest pesticide removal was found to be 74,83% when pesticide concentration of 25 mg L-1 and glucose was used as cosubstrate.

Performance of anaerobic dynamic membrane bioreactor treatment of wastewater from food waste pretreatment: Dissolved organic matter changes and overloading effects

Food waste requires pretreatment before composting due to its high moisture, oil, and salt content. Wastewater from pretreatment (FWPW) has high organic content (COD > 80 g/L) and requires further treatment. The performance and variations in dissolved organic matter (DOM) in an anaerobic dynamic membrane bioreactor (AnDMBR) treating FWPW were investigated along with the impact of the organic loading rate (OLR) of the system. The results showed that, at the stable stage, when the OLR reaches 6.07 kg COD m−3d−1, the average COD removal efficiency was 95.3 %, with a methane production of 0.21 L g−1COD removed. The effluent volatile fatty acid concentration was below 560 mg L−1 and primarily comprised from acetate and propionate. The proportion of DOM with a large (100–500 kDa) and medium (10–100 kDa) molecular weight (MW) in the mixed liquor decreased by 7.0 and 5.5 %, respectively, whereas the MW (> 500 kDa) in the effluent decreased by 14.8 %. The fluorescence spectra revealed only one humic-like peak in the samples, exhibiting a higher fluorescence intensity and humification index in the effluent and mixed liquor than in the influent. At the shock loads stage, the system could withstand a maximum OLR of 11.71 kg COD m−3d−1, and further increases in OLR caused reactor acidification and methane production failure. This study offers new strategies for optimal FWPW treatment and holds promise for guiding the industrial application of AnDMBRs.

Anaerobic treatment of fruit and vegetable wastewater using EGSB: From strategies for regulating over-acidification to microbial community

Fruit and vegetable waste (FVW) are characterized by high-water content. Solid-liquid separation of FVW by crushing-extrusion physical pre-treatment provides fruit and vegetable wastewater (FVWW), and then for anaerobic biological treatment to recover methane, which is considered a cost-effective approach. However, anaerobic treatment of FVWW faces difficulties with low methane productivity and over-accumulation of volatile fatty acids (VFAs). In this study, the expanded granular sludge bed (EGSB) reactor was used for the anaerobic treatment of FVWW and the regulatory strategy to alleviate over-acidification was proposed. When the influent chemical oxygen demand (COD) concentration was 10000 mg/L, the hydraulic retention time (HRT) was 2 days, the organic loading rate (OLR) reached 5 g COD/L/d, the maximum methane productivity reached 301.14 ± 2.32 mL/g COD with a COD removal rate of 96 % ± 2 %. Lower VFAs accumulation was observed when decreasing the influent COD concentration to the same OLR compared with extending HRT, resulting in 38.5 % higher methane productivity. Decreasing the influent COD concentration not only benefited the enrichment of acetogens and hydrogenotrophic methanogens but also specifically enriched syntrophic bacteria. The enhanced syntrophic acetogenesis and hydrogenotrophic methanogens maybe the main reasons for promoting the degradation of propionate and butyrate and improving the methane productivity.

Recent Advanced Biological Wastewater Treatment Technologies

Conventional wastewater treatment approaches such as activated sludge processes often rely on energy-intensive and resource-demanding processes. However, emerging technologies offer promising solutions to improve the sustainability of wastewater management. This review examines three innovative technologies, including anaerobic membrane bioreactor (AnMBR), partial nitritation/anammox (PN/A), and microalgae-based processes, and their potential advantages over traditional methods. AnMBR combine membrane filtration with anaerobic biological treatment, enabling high-quality effluent, reduced sludge production, and a compact footprint. The PN/A is a two-step biological process that can achieve efficient nitrogen removal with lower aeration requirements. Microalgae-based systems leverage photosynthetic organisms to remove nutrients and organic matter while generating biomass for potential resource recovery. Each alternative technology has strengths and limitations that must be carefully evaluated based on the site-specific factors, such as wastewater characteristics, treatment objectives, and local conditions. Continued research and technological development is necessary to address the remaining technical and economic barriers impeding their wider adoption. Integrating multiple alternative technologies in a treatment train can help optimize overall performance by leveraging the complementary advantages of different processes. This holistic approach can advance the sustainable wastewater management, promoting resource efficiency, energy savings, and environmental protection.

Quantification and spatial distribution of the bioenergetic potential from slaughterhouses effluents biogas generation across Brazil

This study describes the estimation of biogas energy use from the anaerobic treatment system of cattle, swine, and poultry slaughter units in Brazil. The simulation includes spatial distribution of the results and geographical comparison with other energy matrices and clean sources of electricity generation. The paper is divided into four sections: 1) the database formation, which included a survey and geocoding of cattle, swine and poultry slaughter units across the country; 2) a literature review covering the period from 2000 to 2020 to obtain the average Chemical Oxygen Demand (COD) for each of the effluents; 3) a simulation of electricity generation in the slaughter units, according to their classes and origins; and, 4) the preparation of national distribution maps of slaughter units, national bioenergy potential, by state and by animal origin, comparison maps of Electric Slaughter Units (ESU) using hydroelectric, photovoltaic and wind power plants, as well as the comparison map between the biomass energy use (biogas, sugarcane bagasse and black liquor). The slaughter units bioenergetic potential presented on the maps that the density in the distribution of these units adopts the effluent treatment proposal. Moreover, biogas is considered a decentralized energy matrix, but the density of slaughter units within the work proposal opposes this concept. The results indicated the formation of a corridor with a high potential for electricity generation, coinciding with regions that experience electrical stress in Brazil. In numbers, the potential would be enough to supply a population of up to 900,000 inhabitants, emphasizing ESU as a potential alternative for electricity using biogas. This is because the Brazilian meat market is somewhat consolidated, important worldwide and continuously generates effluents, which can add up to 28% to electricity from biomass. The greatest bioenergy potential was obtained in the Midwest region (1792 MW), followed by the South (1486 MW), Southeast (1278 MW), North (923 MW) and Northeast (236 MW) regions of the country. In terms of clean energy, when comparing the distribution of slaughter units with photovoltaic, wind and hydroelectric plants, a large territorial gain is observed (more uniform distribution in the country) and in relation to the electrical use of biomass, biogas use from ESU would promote it as the second largest matrix representing about 31% of national energy.