Anaerobic Digestion Of Sewage Sludge Research Articles

Soil application potential of post-sorbents produced by co-sorption of humic substances and nutrients from sludge anaerobic digestion reject water

This study introduces a novel soil conditioning approach using humic substances (HSs) and nutrients co-recovered from reject water from sewage sludge anaerobic digestion. For the first time, HSs and nutrients were simultaneously recovered through sorption on low-cost, environmentally inert materials: natural rock opoka (OP) and waste autoclaved aerated concrete (WAAC). This innovative application of OP and WAAC as carriers and delivery agents for soil-relevant substances offers potential for resource recovery and soil conditioning. Results indicate that the post-sorption opoka (PS-OP) and post-sorption waste autoclaved aerated concrete (PS-WAAC) effectively release retained HSs at 350–480 μg g⁻1 d⁻1, respectively. These materials also show potential as NPK fertilizers, releasing 280–430 μg g⁻1 d⁻1 N-NH₄⁺, 80–150 μg g⁻1 d⁻1 P-PO₄³⁻, and 270–350 μg g⁻1 d⁻1 K⁺. Additionally, PS-OP demonstrated promising fungicide properties, reducing P. diachenii growth by 31% at a concentration of 1 g L⁻1. A two-way ANOVA indicated that the effects of PS-OP and PS-WAAC on soil physicochemical and biological parameters varied with plant species. Both post-sorbents improved the quality of soil collected from sand mining area, increasing cation exchange capacity by 7%–85% and organic matter content by 10%–58%. They also enhanced the functional potential of soil microbial communities, increasing their metabolic activities by 23%–36% in soils sown with clover and by 33%–39% in soils sown with rapeseed. An opposite effect was observed in soils sown with sorghum, suggesting these amendments may not universally act as plant biostimulants. The effectiveness of these post-sorbents in enhancing plant growth varied depending on plant species and the mineral base of the post-sorbent. PS-OP increased the total length of clover and sorghum by 41% and 36%, and their fresh biomass by 82% and 80%, respectively. In turn, PS-WAAC increased the total length of clover and sorghum by 76% and 17%, and their fresh biomass by 29% and 15%, respectively. It was notably more effective than PS-OP for rapeseed. This study proposes a strategy to decrease reliance on non-renewable resources and costly sorbents while minimizing environmental impact. It shows that PS-OP and PS-WAAC can enhance soil quality, microbial activity, and plant growth. Given their origins, these amendments are recommended for soil remediation, particularly in degraded areas. Future research should focus on optimizing their application across various plant species to maximize effectiveness.

Enhanced performance and economic feasibility of sewage sludge digestion using a two-stage anaerobic digestion with a dynamic membrane and alkaline-thermal pretreatment

This study suggests a high-rate sewage sludge anaerobic digestion (AD) process. An alkaline-thermal pretreatment and a dynamic membrane (DM) were used to enhance AD efficiency and economic feasibility in a two-stage system. The effect of pretreatment on volatile fatty acid (VFA) production in the acidogenic phase was investigated at various hydraulic retention times (HRT). After optimizing the acidogenic phase condition (HRT of 3 days), single- and two-stage AD processes with DM modules were operated simultaneously to compare performance. The highest methane production rates of 0.69 L/L/d for single-stage AD and 1.10 L/L/d for two-stage AD were observed at a total HRT of 12 days. Phase separation enhanced the growth of acetoclastic methanogens. A techno-economic analysis showed that the two-stage AD system would achieve a positive net present value within 2 years. This study demonstrated the feasibility of high-rate AD systems for sewage sludge using DM, alkaline-thermal pretreatment, and phase separation.

Feasibility of biogas upgrading at a WWTP after pre-treatment application: Techno-economic assessment validation with pilot test data

Improving the efficiency of anaerobic digestion (AD) of sewage sludge (SS) is a critical step toward the achievement of energy neutrality in wastewater treatment plants (WWTPs), as required by the European Green Deal. This study used a comparative techno-economic assessment (TEA) to evaluate the feasibility of producing biomethane, at a WWTP, through upgrading biogas with a double-stage permeation membrane plant. The biogas was originally generated from the AD of a mixture of primary sludge (PS) and either raw or pre-treated waste activated sludge (WAS), where biological or thermo-alkali pre-treatments were applied to increase the WAS intrinsic low degradability.The TEA was supported by the results of pilot-scale tests, carried out on WAS, which mimicked (i) a traditional mesophilic AD process; (ii) a two-stage AD process, where a temperature-phased anaerobic digestion (TPAD, 3 days, 55 °C + 20 days, 38 °C) was performed to biologically pre-treat WAS; (iii) a traditional mesophilic AD process preceded by a thermo-alkali (4 g NaOH/100 g TS, 90 °C, 90 min) pre-treatment.The TEA was carried out in two phases. In the first, the minimum size of the WWTP capable of making the costs necessary for the implementation of the biogas upgrading plant equal to the revenues coming from selling biomethane (at 62 €/MWh) in 10 years was calculated in the absence of pre-treatments. It resulted of 500,000 equivalent inhabitants (e.i.). In the second phase, for the WWTP size found previously, the effect of either biological or thermo-alkali pre-treatments on the economic balance was evaluated, that is the gain (or the loss) associated to the selling of biomethane, compared to the reference price of 62 €/MWh. It was found that the TPAD increased the biogas productivity by only 23.6%, too little to compensate the amount of heat necessary for the pre-treatment and the purchase cost of the additional reactor. Conversely, the thermo-alkali pre-treatment, which enhanced the WAS biogas productivity by 110%, increased the biomethane revenues by approx. 10 €/MWh, compared to the scenario without pre-treatments. This study offers useful data to WWTP managers who want to introduce WAS pre-treatments, combined with interventions for biogas upgrading, in a new or existing sludge line of a WWTP.

Energy recovery from poultry manure in the process of semi-continuous anaerobic co-digestion with sewage sludge

In the face of significant legislative changes in renewable energy sources and conservation of natural resources, bio-waste must be managed efficiently. Developing wastewater treatment technologies generate a large amount of sewage sludge requiring appropriate use. A well-known and practiced process is the anaerobic digestion of sewage sludge, but due to its characteristics, the production of biogas is not sufficient enough to ensure the energy self-sufficiency of a wastewater treatment plant. Another waste whose management is a challenge is poultry manure, whose production in Poland is high due to intensive poultry farming. This study investigated the possibility of co-digestion of poultry manure and sewage sludge and its effect on increasing biogas production compared to processing sludge alone. The process was performed in two continuously stirred-tank glass reactors at mesophilic conditions, with 20 days of hydraulic retention time. In the first stage, the batch assay with sewage sludge was applied to promote the development of an anaerobic community. The second stage involved feeding the reactors on a semi-continuous regime with sewage sludge for the control sample and a mixture with the gradual addition of poultry manure from 2.5 % to 60 %. During the experiment, the most important parameters affecting the process and the quantity and quality of the obtained products in the form of biogas and digestate were monitored. The study’s results indicated an advantage of the co-digestion process of poultry manure and sewage sludge over the digestion of sludge alone in terms of process efficiency. The highest biogas production was obtained with a co-substrate ratio of 42 % manure to 58 % sewage sludge (ratio based on volatile solids). Co-digestion had no significant effect on gas quality; in both cases, the methane content was more than 60 %. Moreover, a digestate with fertilizer potential was obtained for each sample.

Low-intensity magnetization pretreatment to enhance biomethane generation and the abundance of key microorganisms for anaerobic digestion of sewage sludge

This work explores the impact of static magnetic field (SMF) intensity on biomethane production from anaerobic digestion (AD) of sewage sludge. Two different SMF intensities (20 mT and 1.5 T) were applied to magnetize the sludge destined to the AD process. The magnetic pretreatment at 20 mT was particularly effective, as it increased biomethane production by 12.7 % compared to the control test. On the contrary, exposing the sludge to 1.5 T adversely affected biomethane production, resulting in a 15.1 % decrease. The positive correlation observed between low-intensity SMF exposure and enhanced biomethane yield, in contrast to the inhibitory effect of high-intensity SMF, suggests the existence of an optimal intensity threshold within the lower range for maximizing methane production. The impact of magnetic pretreatment on the anaerobic microbial community was investigated through high-throughput sequencing analysis of magnetized sludge samples. This approach enabled the identification of specific shifts in microbial populations associated with SMF exposure, thereby elucidating the role of SMF in modulating key microbial communities for the AD process. The findings of this study provide insights into the potential mechanisms underlying these responses and underscore the potential of SMF application for improving the anaerobic valorization of sewage sludge.

Improving the anaerobic digestion of sewage sludge by adding cobalt nanoparticles

ABSTRACT This work evaluated the effects of cobalt nanoparticles (CoNPs) (0.025–7 mg/gVS) on the intensification of sewage sludge anaerobic digestion (AD) using biochemical methane potential (BMP) tests. This study was motivated by the need to improve the efficiency and stability of anaerobic digestion of sewage sludge, a critical process in waste management and renewable energy production. The effects at doses less than 2 mg/gVS were not substantial, but 3–7 mg/gVS improved the performance. The maximum biogas yield was 232 mL/gVS (at a dose of 7 mg/gVS), whereas it was 132 mL/gVS in the control (zero dose). Similarly, the reductions in the volatile solids and methane contents reached maxima of 16 and 74.3%, respectively. The analyses of volatile fatty acids, redox potential, and electron transfer system activity indicated that the addition of CoNPs stimulated the early stages of AD. Finally, acetate consumption and the increase in CH4 content suggested that CoNPs positively affected system stability and acetoclastic methanogenesis. That is, CoNPs effectively intensified the behaviour and stability of the anaerobic process. The novelty of this research lies in the comprehensive evaluation of the effects of CoNPs across a wide range of doses on sewage sludge AD, providing new insights into the optimisation of this process for increased biogas production and organic matter reduction.

Novel bioaugmentation process to enhance anaerobic digestion of municipal sewage sludge

The imperative shift to a circular economy, driven by population growth, urbanization, and economic development, highlights the importance of recovering resources from waste streams. Anaerobic digestion (AD) is promising for stabilizing organic wastes and producing renewable methane-rich biogas. However, AD faces challenges, including operational instability, fatty acids accumulation, ammonium inhibition, and low yields. This study investigates the applicability of a novel Ydro Process® bioaugmentation on municipal sewage sludge anaerobic digestion. The process led to pre-hydrolysis of sewage sludge, as evidenced by an increase in volatile fatty acids (VFA), from 134 mg HAc/L in raw TWAS to 997 mg HAc/L in augmented sludge and improved soluble chemical oxygen demand (SCOD) yields. Additionally, the viscosity decreased by 41 % in the Ydro Process® bioaugmented sludge. Biogas production significantly increased from 732 mL/day to 1338 mL/day during semi-continuous digestion, with methane yield rising from 116 mL/g TCODadded to 218 mL/g TCODadded. Microbial analysis revealed an enhanced population of hydrogenotrophic methanogens.

Prioritizing industrial wastes and technologies for bioenergy production: Case study

Energy supply stability in the industrial sector is crucial to maintain operational efficiency and avoiding costly disruptions. In the face of pressing environmental challenges, transitioning to sustainable, efficient, and eco-friendly energy sources is imperative. This study aims to assess the potential of industrial waste for bioenergy production in Khorasan Province, Iran, addressing the research gap of developing a comprehensive framework of criteria that was lacking in previous studies. Employing a combined technology and material assessment methodology, the research began with the collection and analysis of questionnaires to identify and weight critical criteria using Shannon Entropy and expert insights. The Additive Ratio Assessment method was then used to rank various types of industrial waste and technologies according to established value criteria. The Findings reveal that anaerobic digestion of organic waste emerges as the most viable bioenergy solution with an 85 % desirability score, followed by anaerobic digestion of sewage sludge and gasification of plastic waste, scoring 77.31 % and 69.41 %, respectively. The innovative aspect of this study is the development and implementation of five novel evaluation criteria, including process temperature, technology lifetime, production cost, waste collection cost, and waste separation cost, which have not been previously applied to the assessment of industrial waste for renewable energy production, especially in developing countries.

Succession from acetoclastic to hydrogenotrophic microbial community during sewage sludge anaerobic digestion for bioenergy production.

To assess microbial dynamics during anaerobic digestion (AD) of sewage sludge (SWS) from a municipal Wastewater Treatment Plant (WWTP), a Biochemical Methane Potential (BMP) assay at 37°C under mono-digestion conditions was conducted. Utilizing the Illumina MiSeq platform, 16S ribosomal RNA (rRNA) gene sequencing unveiled a core bacterial community in the solid material, showcasing notable variations in profiles. The research investigates changes in microbial communities and metabolic pathways to understand their impact on the efficiency of the digestion process. Prior to AD, the relative abundance in SWS was as follows: Proteobacteria > Bacteroidota > Actinobacteriota. Post-AD, the relative abundance shifted to Firmicutes > Synergistota > Proteobacteria, with Sporanaerobacter and Clostridium emerging as dominant genera. Notably, the methanogenic community underwent a metabolic pathway shift from acetoclastic to hydrogenotrophic in the lab-scale reactors. At the genus level, Methanosaeta, Methanolinea, and Methanofastidiosum predominated initially, while post-AD, Methanobacterium, Methanosaeta, and Methanospirillum took precedence. This metabolic transition may be linked to the increased abundance of Firmicutes, particularly Clostridia, which harbor acetate-oxidizing bacteria facilitating the conversion of acetate to hydrogen.

Life cycle assessment of power-to-methane and renewable methane production technologies

The role of renewable energy, such as biogas or renewable methane is important for decarbonisation of the energy sector. This study analyses environmental impacts of renewable methane production from seven different technologies including wind- and solar-powered electrolysis and methanation with direct air-captured CO2, anaerobic digestion of sewage sludge, pig and dairy manure, and food waste, as well as landfill gas upgrade. Life cycle impact assessment using ReCiPe 2016 method found that sewage sludge system exhibited the highest global warming impact due to electricity consumption during digestate dewatering by centrifuge, whereas anaerobic digestion of pig manure in a covered lagoon with digestate dewatering in Geotube® demonstrated the lowest impact. When centrifuge and Geotube® were compared for the same production system, Geotube® had 78 % lower global warming impact than dewatering with centrifuge. Overall, less energy and resource requirements, and environmental credits assigned to avoided products, such as mineral fertiliser and air-captured CO2 led to lower impacts, where seven out of 11 production systems presented lower global warming impacts than natural gas, indicating potential reduction of emissions by substituting natural gas with renewable methane. Increasing efficiency of wind and solar electricity appeared to be more sensitive than increasing efficiency of electrolyser, while transport distance of the landfill gas had significant effect on the global warming impacts. Production of renewable methane from waste can be promoted by various supports, such as carbon credits, amendment of national gas standard, and waste management policies for energy recovery.

Statistical modeling and optimization of volatile fatty acids production by anaerobic digestion of municipal wastewater sludge.

Obtaining value-added products from renewable resources is limited by the lack of specific operating conditions optimized for the physico-chemical characteristics of the biomass and the desired end product. A mathematical model and statistical optimization were developed for the production of volatile fatty acids (VFAs) by anaerobic digestion of municipal sewage sludge. The experimental tests were carried out in triplicate and investigated a wide range of conditions: pH 9.5, 10.5, and 11.5; temperatures 25°C, 35°C, 45°C, and 55°C; primary sludge with organic loading (OL) of 10 and 14g VS (volatile solids); and digested sludge with 4 and 6g VS. Subsequently, a statistical search was performed to obtain optimal production conditions, then a statistical model of VFA production was developed and the optimal conditions were validated at pilot plant scale. The maximum VFA concentration predicted was 6975mg COD (chemical oxygen demand)/L using primary sludge at 25°C, initial OL of 14g VS, and pH 10.5. The obtained third-degree model (r2 = 0.83) is a powerful tool for bioprocess scale-up, offering a promising avenue for sustainable waste management and biorefinery development.

Comprehensive insights into the impact of magnetic biochar on protein hydrolysis in sludge anaerobic digestion: Protein structures, microbial activities and syntrophic metabolisms

The addition of composite conductive materials is being increasingly recognized as a promising strategy to enhance anaerobic digestion (AD) performance. However, the influence of these materials on protein hydrolysis has been poorly documented. Here, a novel magnetic biochar derived from oil sludge and straw was synthesized using different iron sources and successfully applied in sludge AD. Experimental results revealed that magnetic biochar modified by Fe2+ exhibited excellent electron transfer capacity, moderate magnetization, diverse functional groups (e.g. C=O, C-O=O-), and abundant iron distribution. These characteristics significantly enhanced the hydrolysis of tryptophan-like components, leading to increased methane production (144.44 mL gVS−1vs 79.72 mL gVS−1 in the control test). Molecular docking analysis revealed that the binding of magnetic biochar related Fe2+ and Fe3+, onto sludge proteins via hydrogen bond played a key role in promoting subsequent protein hydrolysis. Additionally, the noteworthy conservation of protein structures from α-helix and β-sheet to random coil, along with the breakdown of the amide I-associated C=O group and amide III-related CN and NH bonds following the addition of magnetic biochar, accelerated the degradation of sludge protein. Observation of variations in protease activity, coenzyme F420, electron transfer system (ETS), and conductivity within the AD systems, particularly the enrichment of Methanospirillum and Methanosaeta archaea, as well as the Petrimonas, Comamonas, and Syntrophomonas bacteria, suggested that magnetic biochar facilitated a conducive environment by improving hydrolysis-acidification and the direct interspecies electron transfer (DIET) process for acetoclastic methanogens. Moreover, metabolic pathways further proved that tryptophan metobalism and acetoclastic methanogenesis were both facilitated by magnetic biochar. This study provides an in-depth understanding of the impact of magnetic biochar on protein hydrolysis in sewage sludge AD.

Study on the fate of per- and polyfluoroalkyl substances during thermophilic anaerobic digestion of sewage sludge and the role of granular activated carbon addition

Limited information is available on the removal of per- and polyfluoroalkyl substances (PFAS) in anaerobic digestion (AD). Τhe fate of six PFAS was studied in thermophilic bioreactors in the presence of granular activated carbon (GAC) and voltage application. Reactors with GAC exhibited lower concentrations of volatile fatty acids and higher methane production compared to those with and without the application of voltage. Analysis of PFAS in dissolved and solid phase showed that their distribution was dependent on perfluorocarbon chain length and functional group. Mass balances showed that PFAS were not removed during conventional AD or after applying voltage; however, significant removal (up to 61 ± 8 %) was observed in bioreactors with GAC for perfluoroheptanoic acid (PFHpA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), and perfluorooctane sulfonate (PFOS). Biomass characterization showed that in these bioreactors, the relative abundance of Acinetobacter and Pseudomonas was higher, indicating their potential role in PFAS biotransformation.

Carbon fixation via volatile fatty acids recovery from sewage sludge through electrochemical–pretreatment–based anaerobic digestion

Capturing the carbon in volatile fatty acids (VFA) produced from the anaerobic digestion (AD) of sewage sludge has the potential to not only provide economic benefits but also reduce greenhouse gas production. This study demonstrates a chemical–free method to collect VFA from an AD instead of methane that involves electrochemical pretreatment (EPT) of sludge. Experimental results show that applying 15 V EPT for 45 min enhances acidogenesis and selectively inhibits methanogenesis, leading to a substantial VFA accumulation (2563.1 ± 307.9 mg COD/L) and achieving 2.5 times more carbon fixation than via methane production. Interfacial thermodynamic analysis shows that EPT induces a decrease in both the repulsive electrostatic energy (from 152.9 kT to 12.2 kT) and the energy barrier (from 57.0 kT to 2.6 kT) in the sludge, leading to increased sludge aggregation and entrapment of microorganisms. Molecular docking sheds lights on how the methanogens interacts with the organic matter released from EPT (e.g., alanine–tRNA ligase), showing that these interactions potentially interfere with the proteins that are associated with the activities of the methanogens and the electron transfer pathways, thereby impeding methanogenesis. Integrating EPT into AD therefore facilitates the recovery of valuable VFA and the capture of carbon from freshwater sludge, providing notable economic and environmental benefits in sewage sludge treatment.

Ammonia stripping by in situ biogas self-circulation to upgrade continuous thermophilic and mesophilic digestion of hydrothermal high-solid sludge

High-solid anaerobic digestion of hydrothermal sewage sludge has been developed. In order to upgrade the process by focusing on ammonia inhibition, a simply-equipped stripping system without additional alkali or heat supply was introduced by in situ biogas self-circulation. As the determined limit of total ammonia nitrogen at 1500 mg/L and 1000 mg/L for the mesophilic (MAD) and thermophilic anaerobic digestion (TAD) respectively and stripping rate at 5 L/min, continuous MAD and TAD was conducted in parallel. The stripping system successfully polished up the ammonia inhibition, and methanogenic capability of the TAD was promoted to approximately 90.0 % of the potential. Intermittent stripping mode proved usable. More frequent stripping was inevitable for the TAD as compared to the MAD. Hydraulic retention time below 20 d resulted in failure of the stripping mode due to rapid ammonia generation. Overall, this technology was practical in upgrading high-solid sludge digestion by effective ammonia control.

Continuous co-digestion of sewage sludge and highly concentrated waste bioplastic hydrolyzate without shortening hydraulic retention time

Bioplastics have garnered substantial interest as alternatives to conventional petroleum-based plastics. However, their management and conversion to biogas continues to be significantly challenging. In this study, we evaluated the suitability of various plastics for hydrolysis at 160 °C for 12 h using different solvents. The biogas yield (BGY) of the monomers constituting these plastics and the obtained plastic hydrolyzates was comprehensively evaluated. When water was used as a solvent, 100 % hydrolysis of polylactic acid (PLA) and polybutylene succinate (PBS) was observed. When polybutylene adipate co-terephthalate (PBAT) and polyhydroxybutyrate (PHB) were hydrolyzed with water, the degradation ratio was approximately 30 %; however, using an aqueous lactic acid solution as a solvent improved the degradation ratio to 78 % and 100 %, respectively. In the BGY test of the plastic hydrolyzates, the biogas volumes derived from the hydrolyzates were 563, 461, 337, and 573 mL/g COD-added for PLA, PBS, PBAT, and PHB, respectively. With 1,160 gCOD/L waste PLA hydrolyzate, continuous co-digestion of sewage sludge and the hydrolyzate was conducted. Organic loading rates of sewage sludge and the hydrolyzate were 2.3 and 2.4 gCOD/L/d, respectively. The operation was stable and the methane production volume from the PLA hydrolyzate was 414 L/kgCOD-added. Using highly concentrated PLA hydrolyzate, the hydraulic retention time was 19.3 days, which was only 0.7 days shorter than that of anaerobic digestion of sewage sludge only (20 days). Therefore, highly concentrated PLA hydrolyzate maintains the retention time of normal sewage sludge digestion. Conclusively, the present study has crucial practical implications for plastic waste management.

Auto-tuning data-driven model for biogas yield prediction from anaerobic digestion of sewage sludge at the south-tehran wastewater treatment plant: Feature selection and hyperparameter population-based optimization

In this study, two data-driven models, artificial neural networks and support vector regression (SVR), have been trained and optimized to predict the biogas yield from anaerobic digesters at the South-Tehran municipal wastewater treatment plant. The auto-tuning approach, including feature selection and hyperparameter population-based optimization, was applied through the genetic algorithm (GA) and particle swarm optimization to improve the training models' performance and help them obtain the best input parameters. The Shapley Additive Explanations (SHAP) analysis was also done to interpret models effectively and assign credit for a model's prediction to each feature. The findings demonstrated that biogas prediction using SVR-GA achieved the highest accuracy, with R2 values of 0.725 and 0.773, and RMSE values (regarding normalized datasets) of 0.477 and 0.492 for the train and test, respectively, while requiring the least computational time compared to other models. The auto-tuning technique, by removing the less important inputs, was able to show that temperature, pH, effluent and influent dry solids, effluent volatile solids (VS), and influent VS of waste sludge were the best input parameters for optimal biogas production modeling. The SHAP analysis revealed that VSeff and temperature were two of the most important features affecting biogas production, exhibiting an inverse impact.

Impact Evaluation of Wastewater Treatment Based on the Anaerobic Digestion of Sewage Sludge Using the Life Cycle Assessment Method

All the inputs and outputs of a technical system can be interpreted from an environmental point of view. Using the life cycle assessment (LCA) approach, some changes that are less harmful to the environment can be included in the system. This research aims to evaluate the environmental effects of the wastewater treatment plant (WWTP) in South Tehran, and the LCA method was used in this study. Based on the data of qualitative parameters obtained from the measurement of Tehran province’s water and sewage company, the environmental emissions were calculated and analyzed using SimaPro software (9.0.0) and the standards defined under the ReCiPe 2016-midpoint method. In the ReCiPe 2016 method, the results were expressed in two intermediate levels (including three classes of influence) and final (including 18). The results showed that the treated wastewater and chlorine factors had the most adverse environmental effects. Among the 18 effect classes, the treated wastewater in the class of marine environmental toxicity with the amount of 101.1531 kg 1,4-DCB had the most environmental impacts among other classes. The power consumed by the biogas-burning combined heat and power (CHP) unit in the wastewater treatment (WWT) process reduced the environmental effects in most impact classes. The most adverse environmental effects of the WWT process are related to damage to human health and the ecosystem. According to the findings, the use of CHP systems is suggested for energy saving and also for reducing harmful effects on the environment.

Calibration of a sewage sludge anaerobic digestion model with multiple mineral precipitation for two case studies

Including multiple mineral precipitation in anaerobic digestion models is important to accurately assess the quantity of phosphates (PO4-P) in the liquid phase of digested sludge that can be recovered as a fertilizer in struvite precipitation reactors or by emerging processes such as ion exchange membranes. However, the performance of such processes is highly impacted by the presence of potassium (K+) or calcium (Ca2+) ions, which are most often neglected when validating precipitation models. The novelty of this work was to provide a calibration procedure of an anaerobic digestion model with multiple mineral precipitation that can be used for different case studies and do not focus only on PO4-P but also on K+, Ca2+ Mg2+. To draw this calibration procedure, data from two anaerobic digesters located near Lyon (France) and in the Navarra region (Spain), treating sludge with different biodegradability and concentration ranges of phosphorus (P), calcium (Ca), magnesium (Mg), potassium (K) and soluble inorganic carbon (SIC), have been used. This procedure includes the calibration of the inert COD fraction (fXI,COD) and kinetic precipitation constants (Kr) using Bayesian Monte Carlo techniques. However, a unique parameter set for both anaerobic digesters cannot be identified, leading to site-specific parameters for fXI,COD (0.30 and 0.43), Kr,MgCO3 (274 and 0.4), Kr,CaCO3 (21 and 0.12) and Kr,ACP (270 and 58). The calibrated model was able to estimate PO4-P, Ca2+, Mg2+ and K+ concentration in digested sludge with a relative error below 10 %.

Magnetic porous microspheres enhancing the anaerobic digestion of sewage sludge: Synergistic free and attached methanogenic consortia

The addition of exogenous materials is a commonly reported method for promoting the anaerobic digestion (AD) of sludge. However, most exogenous materials are nano-sized and their use encounters problems relating to a need for continuous replenishment, uncontrollability and non-recyclability. Here, magnetic porous microspheres (MPMs), which can be controlled by magnetic forces, were prepared and used to enhance the methanogenesis of sludge. It was observed that the MPMs were spherical particles with diameters of approximately 100 µm and had a stable macroporous hybrid structure of magnetic cores and polymeric shells. Furthermore, the MPMs had good magnetic properties and a strong solid–liquid interfacial electron transfer ability, suggesting that MPMs are excellent carriers for methanogenic consortia. Experimental results showed that the addition of MPMs increased methane production and the proportion of methane in biogas from AD by 100.0 % and 21.2 %, respectively, indicating the MPMs notably enhanced the methanogenesis of sludge. Analyses of variations in key enzyme activities and electron transfer in sludge samples with and without MPMs in AD revealed that the MPMs significantly enhanced the activities of key enzymes involved in hydrolysis, acidification and methanation. This was achieved mainly by enhancing the extracellular electron transfer to strengthen the proton motive force on the cell membrane, which provides more energy generation for methanogenic metabolism. A careful examination of the variations in the morphology, pore structure and magnetism of the MPMs before and after AD revealed that the MPMs increased the prevalence of many highly active anaerobes, and that this did not weaken the magnetic performance. The microbial community structure and metatranscriptomic analysis further indicated that the acetotrophic methanogens (i.e., Methanosaeta) were mainly in a free state and that CO2-reducing methanogens (i.e., Methanolinea and Methanobacterium) mainly adhered to the MPMs. The above synergistic metabolism led to efficient methanogenesis, which indicates that the MPMs optimised the spatial ecological niche of methanogenic consortia. These findings provide an important reference for the development of magnetic porous materials promoting AD.