In the present study is phytochemicals, trace metals and antimicrobial activity of ethanolic extract of Anacyclus pyrethrum. The phytochemical screening of the crude ethanolic root, stem and leaf extracts showed the positive results of steroids, triterpenes, reducing sugar, sugar, alkaloids, flavonoids, saponin, tannins, anthraquinones and amino acids. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn in plant sample were BDL, 0.03, 0.42, 0.79, BDL, BDL and 0.58 mg kg-1, respectively. The descending order of the metal content in the plant sample were: Zn > Fe > Cu > Cd > Cr = Ni = Pb. Among various part of plant extracts studied for antimicrobial activity, root ethanolic extract showed highest of inhibition than leaves and stem ethanolic extracts.