Anabolic Steroids In Urine Research Articles

Short-column gas chromatography/tandem mass spectrometry for the detection of underivatized anabolic steroids in urine

Short-column (3.5 m) gas chromatography (GC)/tandem mass spectrometry (MS/MS) has been investigated for the detection of structurally related, underivatized anabolic steroids in urine. The approach described here demonstrates the ability to rapidly and qualitatively detect underivatized anabolic steroids in spiked urine matrices. In this approach, underivatized steroids are determined using a short-column GC separation, ionized by positive ion chemical ionization, and detected by selected reaction monitoring MS/MS. This approach permits positive identification of underivatized anabolic steroids based on retention time and the production of characteristic product ions. Preliminary detection limits studies in spiked urine samples showed quantitative results between 2 and 40 ng steroid per milliliter of uterine. The potential advantages of this approach compared to present screening methods based on conventional (30 m) GC/MS are its rapidity and selectivity. Reliable qualitative identification can be performed with a short-column GC/MS/MS analysis of less than 6 min with a reduction in sample preparation time due to the elimination of the derivatization step.

Detection of Androgenic Anabolic Steroids in Urine

Virtually all professional and amateur sporting organizations forbid the use of androgenic anabolic steroids (AAS). The International Olympic Committee, United States Olympic Committee, and more recently the National Collegiate Athletic Association enforce the regulations by conducting urine testing. Over the past 3 years we have conducted about 8000 tests for AAS in urine and discovered several hundred positive cases. AAS when present in urine can be detected by screening and confirmatory tests using gas chromatography-mass spectrometry.

Capillary gas chromatography and mass spectrometry detection of anabolic steroids II

AbstractHigh resolution gas chromatography‐mass spectrometry has been used to detect anabolic steroids in urine. With fused silica capillary columns connected directly to mass spectrometer, the anabolic steroids could be detected after extraction, hydrolysis, and derivatization with a sensitivity better then 1 ppb. In vivo excretion and metabolism has been investigated for androstanolone, methandriol, oxymesterone, quindenione, and boldenone.

Capillary gas chromatographic—mass spectrometric detection of anabolic steroids

A procedure is described for the detection of anabolic steroids in urine by using capillary gas chromatography—mass spectrometry. The method is suitable for doping control and has a sensitivity as low as 1 ppb. The excretion mode and the metabolism of ten commercial anabolic steroids is reported.