Amylase Excretion Research Articles

The twenty-four hour excretion of amylase and lipase in the urine: correlation with the functional state and operative injury of the pancreas.

Ambromovage, Anne M. M.S.; Howard, John M. M.D.; Pairent, Frederick W. Ph.D. Author Information

Amylase and lipase values in normal subjects

In 146 hospitalized individuals (71 men and 75 women), the simultaneous fasting serum amylase and lipase concentrations and the urinary amylase excretion per 24 h were determined. Patients with pancreatic abnormalities or other diseases which might produce abnormal enzyme values were ruled out from this study. Age and sex were found not to influence the parameters under investigation. The various parameters failed to show a distinct correlation. The renal amylase clearance was calculated for the individuals, and the frequency distribution is presented. In 10 normal subjects, the postprandial 3-h urinary amylase excretion was found not to differ from the preprandial value. In 10 normal subjects, the course of the 24-h urinary amylase excretion showed no distinct rhythm. The urinary amylase excretion per 24 h probably constitutes a more reliable parameter than the excretion during a brief period of collection or the fasting amylase concentration.

Clinical significance of urinary amylase determinations in diagnosis of pancreatic diseases

1) For evaluating pancreatic diseases with Wohlgemuth’s method of amylase determination, the significant values lie only above 64 unit, and in the low units (2–8) there is no significance suggesting pancreatic abnormalities. 2) Serial determination on urinary amylase excretion is recommended for diagnosing of chronic pancreatic disorders. 3) It is suggested that urinary amylase is more closely related to pancreatic states than serum amylase from the observations of amylase isozyme pattern.

Comparison of Urinary and Serum Amylase Values Following Pancreatic Stimulation in Patients with and without Pancreatic Disease

Summary Serum amylase concentrations and urinary amylase excretion rates were studied before and after pancreatic stimulation in 23 controls and 31 patients with pancreatic disease. The urinary amylase excretion rate increased substantially after stimulation in 20 of 31 patients with pancreatic disease, or 65%, while serum amylase increases were found in only 6 of the 31, or 19%.

URINARY AMYLASE VS SERUM AMYLASE IN PATIENTS WITH PANCREATIC CARCINOMA.

IN A RECENT publication we presented data indicating that in the presence of pancreatitis the one-hour value for urinary amylase exceeded normal more often, in a larger number of patients, and frequently for a longer period of time, than did the values for serum amylase and serum lipase.¹The encouraging results of those observations and of those made previously by Saxon and co-workers²led us to study the relative abnormality of the results obtained with these tests for these enzymes in pancreatic carcinoma. <h3>Material and Method</h3> The data on tests for serum amylase and lipase and urinary amylase were obtained from the records of 192 patients with pancreatic carcinoma proved by surgical exploration. Review of the case records included a search for evidence of concomitant or ancient pancreatitis as well as pancreatic carcinoma. The urinary excretion of amylase was measured by a modification of the method of Huggins

ONE-HOUR VALUE FOR URINARY AMYLASE IN 96 PATIENTS WITH PANCREATITIS.

In 96 patients with unquestionable pancreatitis the hourly excretion of amylase in the urine was determined on the basis of a 2-hour collection of urine. The values obtained were compared with the values for serum amylase and, in some instances, for serum lipase. The value for serum amylase exceeded the upper limit of normal in only 36% of tests, while the value for urinary amylase exceeded this limit in 74% when both were determined on the same day. When these two tests and the serum-lipase test (all three performed on the same day) gave increased values, the mean values exceeded the upper limit of normal 80% for serum lipase, 120% for serum amylase, and 710% for urinary amylase. Early, when the disease process clinically seemed more acute, the values for serum and urinary amylase were higher than normal in 53% and 78% of tests respectively, as compared with 15% and 54% later, when the acute process had largely subsided. Our observations, therefore, confirm those of earlier investigators that the urinary-amylase test is a much more sensitive reflector of the presence of pancreatitis and of its clinical course than is the serum-amylase or serum-lipase test.

Amylase clearance and excretion during water diuresis.

Summary1. Amylase clearance increases only slightly during water diuresis. 2. Amylase excretion remains constant during water diuresis.

Comparative Value of Serum and Urinary Amylase in The Diagnosis of Acute Pancreatitis

The very great importance of a rise in serum amylase concentration for verifying a suspicion of pancreatitis is generally recognized, as is the difficulty that would be encountered in diagnosis without some such measure. Not so widely appreciated is the fact that the serum amylase often fails us, particularly if the episode is mild or the patient is not seen within the first few days. Clearly, any measure of the outpouring of amylase from the pancreas that improves upon the serum concentration should have great practical value. Though much has been written about urinary amylase, the data and conclusions are conflicting, and in consequence urinary amylase excretion is infrequently used in practice. Since amylase is freely excreted by the kidney it seemed reasonable, on a little reflection, to expect the urinary excretion to give information when the serum concentration could not. This is seen from a consideration of the following

STUDIES ON BLOOD AND URINARY AMYLASE&lt;subtitle&gt;I. A new estimation method of urinary amylase, and amylase excretion from normal human kidney&lt;/subtitle&gt;

STUDIES ON BLOOD AND URINARY AMYLASE: I. A new estimation method of urinary amylase, and amylase excretion from normal human kidney Get access MASAMITU TAMAI MASAMITU TAMAI Fourth Department of Internal Medicine of Tokyo Imperial University Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 31, Issue 3, May 1940, Pages 471–479, https://doi.org/10.1093/oxfordjournals.jbchem.a125973 Published: 01 May 1940 Article history Received: 25 April 1940 Published: 01 May 1940