Many loads on engineering structures had obvious randomness, such as earthquakes, wind, sea waves, uneven pavement, etc. The calculation and analysis of random vibration were complicated and inefficient, which has perplexed the development of engineering technology for a long time. To solve the above problems, this paper proposed a harmonic equivalent excitation method for structural random vibration analysis. The excitation force of multi-frequency action in continuous time interval was converted into harmonic excitation force with the same effect as superposition excitation in each discrete time interval by a harmonic equivalent method. The frequency and amplitude of harmonic excitation force in discrete-time intervals were calculated, which were then taken as the deterministic load input of the structure. The response of the structure was calculated by the conventional structural dynamic analysis method. Finally, the harmonic equivalent excitation method was used to analyze random pavement excitation and its application in the control of time-delay vibration reduction. The results show that the harmonic equivalent excitation method simplifies the calculation form of random vibration analysis without reducing the accuracy, and provides an effective method for analyzing random vibration in the engineering field.