Abstract This study focuses on modulation of lightning and convective vertical structure in the southern Amazon as a function of the South American monsoon V index (VI). Four wet seasons (December–March 1998–2001) of Tropical Rainfall Measuring Mission (TRMM) Lightning Imaging Sensor (LIS) and Precipitation Radar (PR) data are examined together with two wet seasons (2000–01) of ground-based Brazilian Lightning Detection Network (BLDN) data. These observations are composited by VI phase (northerly or southerly) for a region of the southern Amazon and discussed relative to VI-regime environmental characteristics such as thermodynamic instability and wind shear. Relative comparisons of VI-regime convective properties reveal 1) slightly larger (20%–25%) PR pixel-mean rainfall during periods of northerly VI due to increased stratiform precipitation, 2) a factor of 2 or more increase in lightning flash density and the lightning diurnal cycle amplitude during periods of southerly VI, 3) a factor of 1.5–2 increase in the conditional probability of any PR radar reflectivity pixel exceeding 30 dBZ above the −10°C level during periods of southerly VI, and 4) an associated factor of 2 or more increase in southerly VI pixel-mean ice water path, with the ice water path being highly correlated to trends in lightning activity. During periods of southerly VI, convection occurs in an environment of increased thermodynamic instability, weak southeasterly low-level, and deep upper-tropospheric easterly wind shear. During periods of northerly VI, low-level westerly shear opposes stronger deep tropospheric easterly shear in a relatively moist environment of weaker thermodynamic instability, consistent with the occurrence of more widespread stratiform precipitation. The composite results of this study point to 1) regime differences in convective forcing that alter the prevalence of ice processes and, by inference, the vertical profile of latent heating and 2) the utility of lightning observations in delineating convective regime changes.