Metaphors play a crucial role in language, thinking, and communication. Emoji, as modern metaphors, carry rich meanings and significantly impact emotional expression. While spatial metaphors in emotional lexicons are confirmed, empirical evidence for spatial metaphors in emoji is scarce, and their neural mechanisms are largely unexplored. This study used a spatial Stroop task to investigate emoji spatial metaphors and their effects on emotional perception and neural mechanisms. The results showed that when the positive emoji were in the upper visual field, compared with the middle and lower visual field, the ability to perceive positive emotions was significantly enhanced; Conversely, when the negative emotion emoji was in the lower visual field, the perception of negative emotion was significantly enhanced compared to the middle visual field. EEG data indicate that inconsistencies between Emoji spatial positions and emotional valence lead to increased amplitudes of the Late Positive Component (LPC), revealing heightened neural activity.