Amplifier Module Research Articles

A Review of Ku-Band GaN HEMT Power Amplifiers Development.

This review article investigates the current status and advances in Ku-band gallium nitride (GaN) high-electron mobility transistor (HEMT) high-power amplifiers (HPAs), which are critical for satellite communications, unmanned aerial vehicle (UAV) systems, and military radar applications. The demand for high-frequency, high-power amplifiers is growing, driven by the global expansion of high-speed data communication and enhanced national security requirements. First, we compare the main GaN HEMT process technologies employed in Ku-band HPA development, categorizing the HPAs into monolithic microwave integrated circuits (MMICs) and internally matched power amplifier modules (IM-PAMs) and examining their respective characteristics. Then, by reviewing the literature, we explore design topologies, major issues like oscillation prevention and bias circuits, and heat sink technologies for thermal management. Our findings indicate that silicon carbide (SiC) substrates with gate lengths of 0.25 μm and 0.15 μm are predominantly used, with ongoing developments enabling MMICs and IM-PAMs to achieve up to 100 W output power and 30% power-added efficiency. Notably, the performance of MMIC power amplifiers is advancing more rapidly than that of IM-PAMs, highlighting MMICs as a promising direction for achieving higher efficiency and integration in future Ku-band applications. This paper can provide insights into the overall key technologies for Ku-band GaN HPA design and future development directions.

Locking-Fluorescence Signals Regulated CRISPR/Cas12a Biosensor Based on Metal-Organic Framework for Sensitive Detection of Salmonella typhimurium.

The efficient, sensitive, and rapid detection of Salmonella typhimurium (S. typhimurium) in food and food products is important to ensure food safety and health. This study developed a fluorescence biosensing assay that integrated recombinase-aided amplification (RAA) and CRISPR/Cas12a with a zeolitic imidazolate framework-8@fluorescein sodium (ZIF-8@FLS) nanocomposite for the sensitive detection of S. typhimurium. In this approach, using RAA as a preamplification module, CRISPR/Cas12a-AChE as a target recognition and dual-enzyme cascade amplification module, and the prepared ZIF-8@FLS with high porosity and rapid pH responsiveness as a fluorescence signal explosive amplification module, the RAA-CRISPR/Cas12a-ZIF-8@FLS biosensor was constructed. Under optimal conditions, it exhibited an excellent linear relationship for S. Typhimurium, with a sensitive detection limit as low as 1.3 × 102 CFU/mL and could complete sample detection within 2 h relying on the RAA and ZIF-8@FLS explosive fluorescence rapid response, demonstrating its significant advantages in specificity, sensitivity, and reliability in food-borne pathogens detection.

Gysel combiners with high-selectivity filtering characteristics: analysis and design

Abstract This paper provides the details of a novel systematic design methodology for two-way in-phase filtering Gysel splitter/combiner networks with high selectivity, which finds application in high power amplifier modules. It simultaneously realizes a filtering function and a two-way splitter/combiner function. The proposed five-port filtering device, based on the Gysel topology, is transformed into a ring of coupled resonators. A rigorous coupling matrix describing the network is used to synthesize the integrated filtering and combining functions. This general network can be implemented in any of the available filter technologies. In this paper, a few design examples are provided, and a six-pole prototype utilizing compact combline coaxial resonators is demonstrated. The proposed design provides an integrated dual function module, reducing component counts and system complexity. A design was fabricated and tested demonstrating good experimental results.

An inverted tetrahedron-mediated DNA walker for sulfadimethoxine detection.

Aninverted DNA tetrahedron-mediated modular DNA walker was developed for the determination of sulfadimethoxine. The inverted DNA tetrahedron scaffold raises several advantages of recognition module including appropriate lateral space, multiple recognition domains, and cost-effectiveness. The proposed inverted DNA tetrahedron-based recognition module exhibited better binding affinity and kinetics toward target antibiotic than that of other DNA tetrahedron counterparts. Upon specific binding with target, the released bipedal DNA walking strand hops to the signal amplification module and moves stochastically with assistant of nicking enzyme. By coupling these two modules, a good linear relationship between the fluorescence intensity of supernatant and the concentration of sulfadimethoxine was achieved in the range 0.1-100nM, and the limit of detection was 64.7pM. Furthermore, this modular DNA walker had also successfully applied tospiked honey and milk samples with satisfactory recoveries from 91.5 to 108.8%, demonstrating its practical sensing capability.

Research on underwater acoustic detection technology based on optical waveguide resonator cavity

PurposeIn acoustic detection technology, optical microcavities offer higher detection bandwidth and sensitivity than traditional acoustic sensors. However, research on acoustic detection technologies involving optical microcavities has not yet been reported. Therefore, this paper aims to design and construct an underwater acoustic detection system based on optical microcavities and study its acoustic detection technology to improve its performance.Design/methodology/approachBased on the principles of optical microcavity acoustic sensors, a signal-detection circuit was designed to form a detection system in conjunction with a laser, an optical waveguide resonator and an oscilloscope. This circuit consists of two modules: a photodetection module and a filter amplification module.FindingsThe photodetection module features a baseline noise of −106.499 dBm and can detect device spectral line depths of up to 2410 mV. The gain stability of the filter amplification module was 58 dB ± 1 dB with a noise gain of −107.626 dBm. This design allows the acoustic detection system to detect signals with high sensitivity within the 10 Hz−1.2 MHz frequency band, achieving a maximum sensitivity of −126 dB re 1 V/µPa at 800 Hz and a minimum detectable pressure (MDP) of 0.37 mPa/Hz1/2, corresponding to a noise equivalent pressure (NEP) of 51.36 dB re 1 V/µPa.Originality/valueThis study designs and constructs a broadband underwater acoustic detection system specifically for optical waveguide resonators based on the sensing principles of silicon dioxide optical waveguide resonators. Experiments demonstrated that the signal detection module improves the sensitivity of underwater acoustic detection based on optical waveguides.

Engineering of a Multi-Modular DNA Nanodevice for Spatioselective Imaging and Evaluation of NK Cell-Mediated Cancer Immunotherapy.

Granzyme A (GzmA) secreted by natural killer (NK) cells has garnered considerable interest as a biomarker to evaluate the efficacy of cancer immunotherapy. However, current methodologies to selectively monitor the spatial distribution of GzmA in cancer cells during NK cell-targeted therapy are extremely challenging, primarily due to the existence of diverse cell populations, the low levels of GzmA expression, and the limited availability of GzmA probes. Herein we develop a multi-modular, structurally-ordered DNA nanodevice for evaluating NK cell-mediated cancer immunotherapy (MODERN), that permits spatioselective imaging of GzmA in cancer cells through GzmA-induced apurinic/apyrimidinic endonuclease 1 (APE1) inactivation. The MODERN incorporates multiple functional modules, including an APE1-gated recognition module, a photo-activated amplification module, an aptamer-mediated tumor-target module, and a polycatenane DNA module, enabling improved sensitivity and specificity towards intracellular GzmA. The MODERN was activated (on) in cancer cells due to the overexpression of APE1, whereas it remained silent (off) in the NK-treated cancer cells owing to the GzmA-induced APE1 inactivation. Furthermore, we demonstrated that GzmA-induced APE1 inactivation blocks the cellular repair of target cells, resulting in efficient cell death. This MODERN that relies on the specific inactivation of APE1 by GzmA should be beneficial for evaluating the efficacy of cancer immunotherapy.

Mechanical Robustness Analysis of Semiconductors with a Single Needle Probe Card Using Acoustic Emissions

The combination of indentation testing and acoustic emission (AE) is widely used to analyze the fracture toughness of test substrates. In the manufacturing of semiconductor devices this material parameter also plays an important role. During the so-called wafer testing inside a wafer prober small probe tips are pressed onto the chip surface to check its performance. To prevent damaging the chip by that, the fracture toughness and load limit of it has to be defined in a prequalification step. This paper presents a test system that imitates the wafer testing process as close as possible and uses acoustic emission to detect appearing cracks and thereby also the load limit. The frontend of the test setup consists of a modular single needle probe card which can be placed in a wafer prober. Also, the indenter properties (tip diameter, stiffness, etc.) can be adjusted to the probe used later on during productive wafer testing to ensure realistic probing conditions. A piezoelectric sensor and a full strain gauge Wheatstone bridge are implemented close to the single indenter to measure the AEs and the applied contact force respectively. The signal-to-noise-ratios (SNRs) of both sensors are improved with an own analog amplifier module before recording them with an USB oscilloscope. A developed measurement software (LabVIEW) is used to adjust measurement parameters (trigger limit, record length, conversion factors, etc.) and automatically store and separate measurement data from different acoustic events and imprints. To evaluate the result files a second software tool (MATLAB) reads the files out and clusters the recorded events to separate crack signals from electrical and mechanical disturbances. With a prototype first test results are generated and the functionality and accuracy of the measurement concept is proven.

On-Site Multiply Stimulated Self-Confinement of an Integrated DNA Cascade Circuit for Highly Reliable Intracellular Imaging of miRNA and In Situ Interrogation of the Relevant Regulatory Pathway.

Artificial DNA circuits represent a versatile yet promising toolbox for in situ monitoring and concomitant regulation of diverse biological events within live cells. Nonetheless, their performance is significantly impeded by the diffusion-dominated slow reaction kinetics and the uncontrollable off-target activation. Herein, a self-localized cascade (SLC) circuit is reported for the robust and efficient microRNA (miRNA) analysis in living cells. The SLC circuit consists of the cell-specific localization module and the analyte-specific signal amplification module. By integrating the reaction probes of these two modules, the complexity of the system is reduced to realize the responsive co-localization of circuitry probes and the simultaneous cascade signal amplification. Taking advantage of the specifically activated, self-localized, and cascade design, the SLC circuit successfully achieves the robust miRNA-21 (miR-21) imaging and the accurate cells differentiation. Moreover, the reverse regulation mechanism is successfully explored between messenger RNA (mRNA) and miRNA through the engineered SLC circuit and further elucidates the underlying signaling pathways between them. Therefore, the SLC circuit provides a powerful tool for the sensitive detection of intracellular biomolecules and the study of the corresponding cell regulatory mechanisms.

MBHFuse: A multi- branch heterogeneous global and local infrared and visible image fusion with differential convolutional amplification features

Fusing infrared and visible imagery aims to harness their complementary spectral data, enhancing output image quality, sharpness, and content. However, convolutional neural networks (CNNs) are not capable of capturing long-range dependencies, while the Transformer architecture faces the challenge of consuming huge computational resources. To address these critical challenges, this paper proposes a novel framework named MBHFuse, which employs a multi-branch heterogeneous global and local image fusion approach. To effectively extract global and local features, we design a multi-branch heterogeneous encoder module and introduce a differential convolution amplification module (DCAM) to further extract complementary information. Additionally, we devise a new loss function, incorporating multi-branch feature decomposition loss, intensity loss, gradient loss, mean squared error loss, and structural similarity loss, for training the proposed MBHFuse model. Through extensive experiments on public datasets, we demonstrate that the proposed framework outperforms other state-of-the-art (SOTA) methods in both qualitative and quantitative evaluations. Our code will be available at https://github.com/sunyichen1994/MBHFuse.

Ligation initiated self-priming isothermal polymerization enabled nano-signal amplification for APE1 sensing and logical unlocking

In this work, we developed a label-free and one-tube method for apurinic/apyrimidinic endonuclease 1 (APE1) sensing based on ligation initiated self-priming isothermal polymerization (SPIP) and signal amplification of fluorescent copper nanoparticles (CuNPs). This method was rationally proposed with modular design. In target recognition module, APE1 could specifically cleave substrate to release a linker DNA that served the ligation of an adenine/thymine (AT)-rich hairpin and a phosphorothioate-modified hairpin with the assistance of ligase. Then in SPIP amplification and signal transduction modules, once driven by polymerase, enzymatic extension and self-folding alternately occurred on the primary DNA originated from the ligation reaction, which resulted in accumulation of numerous AT-rich templates repeatedly included in the elongated dsDNA products, and ultimately enabled rapid formation of fluorescent CuNPs to output amplified signal. The accurate target identification and efficient signal amplification facilitated the highly selective and sensitive detection of APE1 with limit of detection of 8.6 × 10−4 U/mL. Benefiting from the robustness of this method, the normal cells and tumor cells could be distinguished unambiguously with this method according to the test results of cellular APE1. Moreover, the sensing strategy was employed in the concatenated AND logical operation and molecular keypad unlocking, which further displayed its extensibility and versatility.

Development of a novel PCB electric field sensor with high-resolution

Abstract Electric field sensors with high resolution and high sensitivity have an important engineering application. Developing a compact, cost-effective Electric Field Sensor (EFS) for aerospace applications, this study introduces a novel use of PCB parallel board probes, enhancing affordability and simplicity. A uniquely designed three-stage adaptive signal amplification module quickly adjusts amplification factors, ensuring high sensitivity and resolution even in complex electric field environments. Due to the non-linear response characteristics of the PCB probes in complex electric field environments, the GTEM cells and the high voltage parallel plate method are used to simulate the complex electric field scenarios. In the weak electric field experiment, the proposed EFS achieved a high resolution of 10 mV/m. In the strong electric field experiment, the linearity of fitting between the proposed EFS and commercial NBM-550EFS reached 0.9999. Finally, based on RBFNN, the overall measurement model of EFS achieves the dynamic measurement range of 0-1 kV/m.

A novel LDO circuit with high-speed current detection

Abstract This paper proposes a Low Dropout Regulator (LDO) circuit with high-speed current detection. The current detection circuit utilizes a latch-based structure to expedite comparisons and perform detection. Its primary objective is overcurrent protection in certain practical applications, achieving current limit functionality. The LDO circuit structure can stably provide a 5 V output voltage under a 9 V input voltage, with a maximum output current limit of 70 mA. In practical applications, the circuit can function with a load current of 40 mA, resulting in an output voltage (Vout) of 4.96 V. In the design of the LDO circuit, the Error Amplifier (EA) module departs from conventional internal structures, employing a dual-potential folded cascode common-gate common-source amplifier. This choice enhances parameters such as EA gain and Power Supply Rejection Ratio (PSRR). The gain achieves 98 dB, and the low-frequency PSR reaches 92 dB. Simultaneously, the dual potential configuration significantly economizes the layout area required in practical implementations. The LDO is designed using a 0.18 μm standard CMOS process.

Switching-mode audio amplifier based on a ΔΣ A/D converter

The article describes the design and implementation of a modular hardware platform intended for testing and measuring various configurations of switching-mode audio amplifiers based on sigma-delta modulation. Two single-channel power amplifier modules were designed and manufactured, along with a stereophonic module serving as the basic source of modulated signals. Additionally, measurements were conducted on the fundamental parameters of the completed amplifier, such as harmonic distortion level, dynamic range, and output power. The developed platform serves as a foundation for modifications and further advancement in the technology of building switching-mode audio amplifiers.

Photonic crystal-enhanced fluorescence biosensor with logic gate operation based on one-pot cascade amplification DNA circuit for enzyme-free and ultrasensitive analysis of two microRNAs

The development of sensitive and efficient analytical methods for multiple biomarkers is crucial for cancer screening at early stage. MicroRNAs (miRNAs) are a kind of biomarkers with diagnostic potential for cancer. However, the ultrasensitive and logical analysis of multiple miRNAs with simple operation still faces some challenges. Herein, a photonic crystal (PC)-enhanced fluorescence biosensor with logic gate operation based on one-pot cascade amplification DNA circuit was developed for enzyme-free and ultrasensitive analysis of two cancer-related miRNAs. The fluorescence biosensor was performed by biochemical recognition amplification module (BCRAM) and physical enhancement module (PEM) to achieve logical and sensitive detection. In the BCRAM, one-pot cascade amplification circuit consisted of the upstream parallel entropy-driven circuit (EDC) and the downstream shared catalytic hairpin assembly (CHA). The input of target miRNA would trigger each corresponding EDC, and the parallel EDCs released the same R strand for triggering subsequent CHA; thus, the OR logic gate was obtained with minimization of design and operation. In the PEM, photonic crystal (PC) array was prepared easily for specifically enhancing the fluorescence output from BCRAM by the optical modulation capabilities; meanwhile, the high-throughput signal readout was achieved by microplate analyzer. Benefiting from the integrated advantages of two modules, the proposed biosensor achieved ultrasensitive detection of two miRNAs with easy logic gate operation, obtaining the LODs of 8.6 fM and 6.7 fM under isothermal and enzyme-free conditions. Hence, the biosensor has the advantages of high sensitivity, easy operation, multiplex and high-throughput analysis, showing great potential for cancer screening at early stage.

A Methylation-Gated DNAzyme Circuit for Spatially Controlled Imaging of MicroRNA in Cells and Animals.

Epigenetic modification plays an indispensable role in regulating routine molecular signaling pathways, yet it is rarely used to modulate molecular self-assembly networks. Herein, we constructed a bioorthogonal demethylase-stimulated DNA circuitry (DSC) system for high-fidelity imaging of microRNA (miRNA) in live cells and mice by eliminating undesired off-site signal leakage. The simple and robust DSC system is composed of a primary cell-specific circuitry regulation (CR) module and an ultimate signal-transducing amplifier (SA) module. After the modularly designed DSC system was delivered into target live cells, the DNAzyme of the CR module was site-specifically activated by endogenous demethylase to produce fuel strands for the subsequent miRNA-targeting SA module. Through the on-site and multiply guaranteed molecular recognitions, the lucid yet efficient DSC system realized the reliably amplified in vivo miRNA sensing and enabled the in-depth exploration of the demethylase-involved signal pathway with miRNA in live cells. Our bioorthogonally on-site-activated DSC system represents a universal and versatile biomolecular sensing platform via various demethylase regulations and shows more prospects for more different personalized theragnostics.

EAFNet: Extraction-amplification-fusion network for tiny cracks detection

Tiny cracks are often overlooked in the inspection process, causing huge economic losses and dangerous accidents. Therefore, tiny cracks should be detected in a timely and accurate manner to eliminate the disease at the initial stage. Inspired by the fact that humans are more likely to capture conspicuous information when observing objects, we propose a novel three-stage Extraction-Amplification-Fusion network (EAFNet). Specifically, in the extraction stage, we utilize an effective backbone network for feature extraction of tiny cracks. In the amplification stage, we design a Tiny Feature Amplification (TFA) module to amplify the extracted features. In the fusion stage, we propose a Two-Branch Fusion (TBF) module to fully fuse the feature maps at different resolutions. To make tiny crack information more ‘conspicuous’, we propose an activation function TinyReLU to enhance the contrast of the tiny cracks with the background. In addition, we construct a Tiny Crack (T-CRACK) dataset with six different backgrounds and a Cross-scale Crack (C-CRACK) dataset. On both datasets, EAFNet achieves an advantage over the existing 8 advanced networks. The two datasets are available at: https://github.com/EAFNet/EAFNet.

Compact hundred-watt level picosecond laser system based on tapered double cladding fiber-single crystal fiber hybrid amplifier

This paper introduces a compact picosecond laser system using tapered double cladding fiber-single crystal fiber hybrid amplification technology for the first time. By modifying the Ginzburg-Landau equation, we established a simulation model and designed a passive mode-locked picosecond oscillator based on a semiconductor saturable absorber mirror. The oscillator outputs laser with a pulse width of 7.75 ps, a center wavelength of 1030.55 nm, and a spectrum width of 0.58 nm. The seed laser is stretched and amplified by the all-fiber front stage, and then injected into the tapered double cladding fiber-single crystal fiber hybrid amplifier. The laser power is amplified from 820 mw to 103.1 W, obtaining a gain of 21 dB. We used zero-phonon line pumping technology in single crystal fiber amplifier to reduce the thermal effects caused by quantum loss, thereby increasing the length of the crystal and increasing the power loading capacity. We designed a single crystal fiber amplification module using Yb:YAG with 60 mm long, 1 mm diameter and a 1 at.% doping rate for the first time. Finally, a hundred-watt level picosecond laser output with a repetition frequency of 26.33 MHz, an average power of 103.1 W, a pulse width of 244.72 ps, a spectrum width of 1.16 nm, and a beam quality of M2 x = 1.368 and M2 y = 1.511 was obtained. To the best of our knowledge, this is the highest picosecond laser output power for an all-fiber front-stage combined with a single-stage single crystal fiber amplifier.

Fluorophore multimerization on a PEG backbone as a concept for signal amplification and lifetime modulation

Fluorescent labels have strongly contributed to many advancements in bioanalysis, molecular biology, molecular imaging, and medical diagnostics. Despite a large toolbox of molecular and nanoscale fluorophores to choose from, there is still a need for brighter labels, e.g., for flow cytometry and fluorescence microscopy, that are preferably of molecular nature. This requires versatile concepts for fluorophore multimerization, which involves the shielding of dyes from other chromophores and possible quenchers in their neighborhood. In addition, to increase the number of readout parameters for fluorescence microscopy and eventually also flow cytometry, control and tuning of the labels’ fluorescence lifetimes is desired. Searching for bright multi-chromophoric or multimeric labels, we developed PEGylated dyes bearing functional groups for their bioconjugation and explored their spectroscopic properties and photostability in comparison to those of the respective monomeric dyes for two exemplarily chosen fluorophores excitable at 488 nm. Subsequently, these dyes were conjugated with anti-CD4 and anti-CD8 immunoglobulins to obtain fluorescent conjugates suitable for the labeling of cells and beads. Finally, the suitability of these novel labels for fluorescence lifetime imaging and target discrimination based upon lifetime measurements was assessed. Based upon the results of our spectroscopic studies including measurements of fluorescence quantum yields (QY) and fluorescence decay kinetics we could demonstrate the absence of significant dye-dye interactions and self-quenching in these multimeric labels. Moreover, in a first fluorescence lifetime imaging (FLIM) study, we could show the future potential of this multimerization concept for lifetime discrimination and multiplexing.

Water-cooling-based and low-cost qPCR device for rapid nucleic acid analysis

Real-time quantitative PCR (qPCR) is a sought-after method for quantifying nucleic acids. A series of commercial instruments based on qPCR has been developed and applied to detect several diseases. However, commercial instruments have some limitations in terms of cost, bulk, and efficiency. Herein, we proposed a novel qPCR device using water-cooling technology to shorten the period of the PCR reaction. The device was made of an amplification module with a novelty water-cooling-based PCR chip, a custom-made fluorescence detection module, and a control module based on economical off-the-shelf electronics. All these modules were assembled in a 3D-printed frame with a size of 11 cm × 9.7 cm × 8.9 cm and a weight of 0.5 kg. With the help of water-cooling technology, the total time required to complete a PCR reaction was shortened, with a maximum cooling rate of 12 ℃/s, which is much faster than the rate of conventional cooling methods. Compared with commercial qPCR instruments, our device's cost, efficiency, and portability have been significantly optimized. To test the proposed device, a qPCR experiment was successfully performed with cDNA of Vesicular stomatitis virus at concentrations ranging from 0.005 μg/mL to 50 μg/mL.

A Compact GaN Power Amplifier Module for New Generation Cellular Basestations

This paper presents a compact class-AB Power Amplifier Module (PAM) designed for new generation massive Multiple Input Multiple Output (MiMo) cellular base stations. The module is designed at the center frequency of 3.5GHz targeting Long Term Evolution (LTE) and 5G New Radio (NR) bands. The module is a hybrid design that incorporates a Gallium Nitride (GaN) High Mobility-Electron Transistor (HEMT) die, discrete components-based input, and output matching networks. The entire design is realized on an 8.5 x 5.2 mm, 2-layer Rogers4003C substrate. The module is assembled on a PCB as an open-top for post-characterization tuning. The small signal and large signal measurements are in quite good agreement. The measurement results show that the amplifier is unconditionally stable, the input return loss is 12.2 dB, the output return loss is 7.7 dB, and the small signal gain is 13.4 dB. The saturated output power is 33.3 dBm with a Power Added Efficiency of 20.1%. The small signal gain drops to 11.2 dB at around 22 dBm of input power due to GaN technology’s intrinsic soft compression characteristics.