Amounts Of Reagents Research Articles

A Novel Pot-Economy Approach to the Synthesis of Triantennary GalNAc-Oligonucleotide

N-Acetylgalactosamine (GalNAc) is an efficient and multifunctional delivery tool in the development and synthesis of chemically modified oligonucleotide therapeutics (conjugates). Such therapeutics demonstrate improved potency in vivo due to the selective and efficient delivery to hepatocytes in the liver via receptor-mediated endocytosis, which is what drives the high interest in this molecule. The ways to synthesize such structures are relatively new and have not been optimized in terms of the yields and stages both in lab and large-scale synthesis. Another significant criterion, especially in large-scale synthesis, is to match ecological norms and perform the synthesis in accordance with the Green Chemistry approach, i.e., to control and minimize the amounts of reagents and resources consumed and the waste generated. Here, we provide a robust and resource effective pot-economy method for the synthesis of triantennary GalNAc and GalNAc phosphoramidite/CPG optimized for laboratory scales.

In Situ Synthesis of Iron-Based Porphyrin Metal-Organic Frameworks for the Baeyer-Villiger Oxidation Using Air as an Oxidant.

Iron-based porphyrin metal-organic frameworks (PMOFs) are a new type of material with good stability, catalytic activities, and reusability, which have broad application prospects. However, the synthesis of iron-based PMOFs with different metalloporphyrin units requires a considerable amount of reagents and time. Herein, an in situ synthesis method was developed to simplify the synthetic process of iron-based PMOFs. A series of highly stable iron-based PMOFs (Msitu-PMOF-3(Fe) (M = Fe, Co, Ni, Cu, Pd)) were synthesized in one pot without the preparation of metalloporphyrin ligands in advance. This synthesis method is simpler and more efficient, saving considerable time and material costs. The resultant Msitu-PMOF-3(Fe) exhibits a high tolerance to aqueous solutions with pH ranging from 0 to 11, as well as various organic solvents. The catalytic experiments show that both Fesitu-PMOF-3(Fe) and Cositu-PMOF-3(Fe) can catalyze the Baeyer-Villiger oxidation of cyclohexanone using air as an oxidant at 35 °C. Moreover, when the mixtures of Fesitu-PMOF-3(Fe) and Cositu-PMOF-3(Fe) were employed as catalyst, they can exert a cooperative catalysis effect, resulting in a significantly enhanced catalytic activity compared to a single component. The mild reaction condition, high catalytic efficiency, and excellent selectivity endow this catalytic system with a good application prospect. This work not only provides a new approach for the synthesis of iron-based PMOFs but also offers guidance for the Baeyer-Villiger oxidation reactions using air under mild conditions.

Effect of N,N′-Methylenebisacrylamide on properties of porous semi-IPN hydrogels from Acrylamide, Maleic Acid, and its use for Urea absorption

Hydrogel is a three-dimensional polymer that can absorb large amounts of reagents while maintaining structural integrity. This material has been applied in many fields especially in smart agriculture. To improve the economic viability, the reusability of hydrogels in agricultural engineering over multiple cycles of adsorption and desorption is an urgent requirement. This can be solved if the crosslinker is used properly. Therefore, in this work, a series of porous semi-IPN hydrogels based on linear polyacrylamide, acrylamide, maleic acid, and N,N′-methylenebisacrylamide (MBA) were synthesized. The hydrogels were evaluated for the impact of MBA content on the characteristics and applicability as a urea fertilizer carrier. The chemical composition, morphology, mechanical, and rheological properties, swelling behavior, urea absorption and desorption of hydrogels with crosslinker content in the range of 0.5-2.0 % were investigated. The porous structure was confirmed by scanning electron microscopy images. Changing the MBA content significantly affected all characteristics of the hydrogels. In particular, increasing the MBA content decreased the equilibrium swelling ratios in all investigated environments. The maximum amount of urea loaded into the hydrogel was also reduced from 435.88 to 188.50 mg/g. This increase also changed the swelling mechanism from non-Fickian to Fickian, whereas the urea release mechanism changed from Fickian to non-Fickian. Finally, the hydrogels demonstrated stability in soil over multiple cycles of water absorption and release. This study provides valuable insights into designing a semi-IPN hydrogel with desired properties that meet the application requirements of modern farming techniques.

One-step simultaneous and efficient extraction of multiple active compounds from Lycium barbarum by aqueous three-phase system: Focusing on polysaccharides

In the past few years, the aqueous three-phase system (A3PS) has attracted a lot of attention due to its effectiveness in the efficient separation. Polysaccharides, carotenoids and polyphenols were separated simultaneously from Lycium barbarum by an aqueous three-phase system (A3PS) formed by acetonitrile/betaine/K2HPO4 as a novel extraction technique. The system is characterized by small amount of machine reagents, few consumables and simple extraction steps. The effects of acetonitrile, betaine and K2HPO4 concentrations on the extraction efficiency were investigated by Response Surface Methodology. The optimum composition of A3PS were 26.2 % (w/w) acetonitrile, 10.2 % (w/w) betaine and 27.6 % (w/w) K2HPO4. Under this condition, the extraction efficiencies of carotenoids, polyphenols, and Lycium barbarum polysaccharides (LBP) were 42.12±0.10 %, 88.61±0.95 % and 84.63±0.46 %, respectively. The obtained LBP was characterized by several technologies. The LBP was proved to consist of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose in a proportion of 4.14:0.80:4.00:4.43:22.20:39.9:12.44:3.22:8.46:0.41. The molecular weight of LBP was 12554 Da. In addition, LBP exhibited excellent antioxidant activity. In general, as a rapid developing method of extraction and separation, A3PS had potential application in the simultaneous separation of multiple substances.

A novel micromixer based on coastal fractal for manufacturing controllable size liposome

The traditional lipid preparation methods are complex, time-consuming, and consume a large amount of reagents, increasing costs and difficulties. Although microfluidic technology is considered a promising solution, achieving controllable liposome production with a simple and inexpensive microfluidic mixing device remains an important problem. This paper presents a wall-type micro-mixer based on coastal zone fractals. Four parameters related to the geometric shape of the coastline fractal in the microchannel are used as design variables, and the mixing index is the objective function. Single-objective optimization numerical analysis of the primary wall-type fractal baffle micromixer under four Reynolds numbers conditions yields the optimal structural configuration. Visualization experiments verify the correctness and accuracy of the numerical simulation, and the optimized mixer is used to produce liposomes. The results show that the micro-mixer with the optimal double-sidewall cross arrangement enhances chaotic convection and improves mixing efficiency. At Re = 0.1 and Re = 100, the mixing efficiency reaches 99%, 50.44% higher than the reference design. By changing the relative flow rates of lipid and aqueous solutions, microfluidic blank liposomes with a particle size of 165.12 ± 11.6 nm and a polydispersity index of 0.35± are obtained. This wall-type fractal micro-mixer has broad application prospects due to its high mixing efficiency.

Removal of contaminants in paper dyeing wastewater by Fenton and photo-Fenton processes with biological treatment

Due to the production of huge quantity of potentially toxic and recalcitrant pollutants, which cannot be completely decomposed with conventional biological wastewater treatment processes, in the paper industry, reliable treatment processes are highly required. This study conducted effective experiments of different series of processes to decompose persistent contaminants in the paper wastewater under controlled conditions of the used processes to meet the effluent quality standards of Korea Ministry of Environment in terms of CODMn (< 130 mg/L). For Biological treatment, Fenton, and photo-Fenton processes, various operational parameters were adjusted to determine the optimal conditions for CODMn value. Due to large quantity of refractory contaminants in the wastewater, single treatment process was not enough to meet the requirement of CODMn. Herein, we achieved the goal with two approaches: (1) secondary Fenton oxidation after biological and primary Fenton treatments, and (2) photo-Fenton treatment after biological treatment alone. The secondary Fenton oxidation process is an additional process operated under the same condition of the primary Fenton treatment to treat the effluent from the primary Fenton treatment. Ultimately, the residual CODMn concentrations of the secondary Fenton and single photo-Fenton treatment were 62.7 and 85.3 mg/L, respectively. However, the secondary Fenton required larger amounts of reagents and produced significant amounts of sludge, which require post-treatment, compared to single photo-Fenton treatment. Therefore, it suggests that the application of photo-Fenton was more efficient than the secondary Fenton in terms of economic feasibility and post-treatment requirement.

Cobalt-Catalyzed Regio-, Diastereo- and Enantioselective Reductive Coupling of 1,3-Dienes and Aldehydes.

Catalytic regio-, diastereo- and enantioselective reductive coupling of 1,3-dienes and aldehydes through regio- and enantioselective oxidative cyclization followed by regio- and diastereoselective protonation promoted by a chiral phosphine-cobalt complex is presented. Such processes represent an unprecedented reaction pathway for cobalt catalysis that enable selective transformation of the more substituted alkene in 1,3-dienes, affording a broad scope of bishomoallylic alcohols without the need of pre-formation of stoichiometric amounts of sensitive organometallic reagents in up to 98 % yield, >98 : 2 regioselectivity, >98 : 2 dr and 98 : 2 er. Application of this method to construction of axial stereogenicity and deuterated stereogenic center provided a wide range of multifunctional chiral building blocks that are otherwise difficult to access. DFT calculations revealed the origin of regio- and stereoselectivity as well as a unique oxidative cyclization mechanism for cobalt catalysis.

A novel colored chitosan-modified polystyrene composite dye nanospheres with enhanced coloring properties for binder-free printing

The limited utilization rate of dyes for coloring coupled with the consumption of large quantities of chemical reagents, can result in acute environmental pollution and waste of resources. Herein, in accordance with the electrostatic attraction between electropositivity of chitosan and anionic dyes, a novel type of chitosan-modified cationic polystyrene nanospheres (PSCS) was creatively synthesized, which can be innovatively employed as a special carrier with an adsorption capacity of up to 197.4mg/g for acid dyes. Consequently, a exclusive pigment identified as colored chitosan-modified polystyrene composite dye nanospheres (PSCS(AR9)) was procured. Simultaneously, the binder-free printing was accomplished by leveraging the intrinsic self-curing property of PSCS(AR9) for screen printing of textiles. Additionally, printed fabrics with PSCS(AR9) exhibited superb darkening effect (the K/S value can be increased by 2 times.) in conjunction with significantly improved hand feel in contrast with the conventional printed fabrics. The last but not least, the rubbing fastness of the printed fabrics using PSCS(AR9) can meet demand of the practical application. Therefore, this work may provide an innovative insight for constructing a new type of environmentally friendly and depth-enhanced printed textile.

Towards high throughput analysis using 96-well plate solid phase extraction to determine sedatives and β-blocker residues in food control monitoring

BackgroundVeterinary drugs are widely used in animal production to prevent infections and treat diseases but, this may cause a risk to consumers. Due to the high number of food samples required to monitor yearly, simple, fast, sensitive and selective analytical methods are needed in control laboratories to ensure consumers safety. Nevertheless, many analytical methodologies available in these laboratories include multiple steps and therefore are time-consuming and hinder the analysis throughput requiring significant amounts of solvents and reagents. ResultsThis work developed a 96-well plate solid phase extraction (SPE) method for the extraction of seven sedatives and a β-blocker in animal kidney by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC−MS/MS). The developed method was validated based on Implementing Regulation (EU) 2021/808 in kidney, meat and fish. The performance characteristics of the validation (1–5 μg kg−1) showed good linearity (R2 > 0.998) while decision limits (CCα) were between 1 and 1.2 μg kg−1. Trueness and precision were determined at three levels (n = 7) and the results showed values ranging from 85 to 103 % and from 1 to 9 %, respectively. The feasibility of the method was demonstrated for the residue control requirement established by EU. Method was applied to 201 samples of kidney, meat and fish. SignificanceThis study is the first to present an optimized and validated holistic method for sedatives and β-blocker using 96-well plate SPE and UHPLC−MS/MS. The method showed good performance in kidney, meat and fish samples being an universal method for any species along the same type of sample. The fast, easy, efficient, reliable and universal method showed high throughput and so reduced the analysis time by nine-fold and the required solvent amount by four times fulfilling the green chemistry principals.

Regarding Bioanalysis Lasting a Few Minutes: Automated Cooling-SPME and Fast-GC for Urinary 2-Phenyl-2-Propanol Monitoring.

An innovative SPME head space GC-MS method, in cooling mode, using a fully automated routine, was developed to detect 2-phenyl-2-propanol, a representative urinary metabolite of cumene. Following an acid hydrolysis and derivatization step with lowered quantities of reagents, acetic anhydride and pyridine, a 30 μm polydimethylsiloxane SPME fiber was used to sample derivatized 2-phenyl-2-propanol, such as benzenemethanol,α,α-dimethyl-acetate, from the headspace. Performances of the method, optimized through experimental design, provide an LOD of 0.034 mg/L and an LOQ 0.10 mg/L, with a short sampling time necessary per sample. The method, developed on standard solutions, will be applied to both occupationally exposed and non-exposed populations.

Plasma-mediated mercury vapor generation after microwave-induced combustion of fish tissue with detection by atomic absorption spectrometry

Mercury determination in fish tissues by high-resolution continuum source atomic absorption spectrometry (HR-CS AAS) was performed using microwave-induced combustion (MIC) for sample preparation, and plasma-mediated vapor generation (PMVG) in a tubular dielectric barrier discharge (DBD) as a sample introduction system. The main goal was to develop a method for Hg determination using very low amount of reagents being at the same time virtually free of interferences. The operational parameters of the PMVG step in the DBD reactor were optimized as follows: 70 mL min−1 Ar as the discharge gas, 20 mm electrode distance, primary voltage at 60 V and 50 μL as digested sample volume (in 0.1 mol L−1 HNO3 matrix). The effect of HNO3 concentration on the PMVG efficiency was studied. No differences in signal intensity were observed for HNO3 concentration up to 0.1 mol L−1, while a signal decrease by 25 % and 40 % was observed when the acid concentration increased to 1 and 5 mol L−1, respectively. Accuracy of the proposed method was evaluated using certified reference material (CRM) of dogfish liver (DOLT-4) and by Hg spikes on the sample prior to MIC. By using 500 mg of sample (or 250 mg of CRM) and 6 mL of 0.5 mol L−1 HNO3 as absorbing solution in MIC, recoveries of 104 % and 97 % were observed for spikes and CRM values, respectively. A limit of detection of 0.16 μg g−1 Hg (160 pg Hg absolute) was obtained. A comparison of the results for Hg determination in the CRM by PMVG-AAS and chemical cold vapor generation inductively coupled plasma mass spectrometry (CVG-ICP-MS) was performed with no significant differences in the results. Finally, an efficient, low-cost and low-volume reagent consumption sample introduction system for Hg determination by AAS was successfully combined with the advantages of MIC and subsequent PMVG by DBD.

High-turbulence fine particle flotation cell optimization and verification

Microfine mineral particles have a small size, light weight, and low inertia, making it difficult for them to deviate from streamlines and collide with bubbles. Conventional flotation operations consume a large amount of reagents and exhibit poor flotation indicators. This study employs computational fluid dynamics (CFD) simulation and hydrodynamic testing to investigate the flow field within a high-turbulence microfine particle flotation machine equipped with a multilayer impeller–stator configuration, and validates the practical application performance of the microfine particle flotation machine through single-batch flotation experiments. Result shows that the impeller region of the traditional mechanical stirring flotation machine has a turbulent energy dissipation rate of 20 m²/s³, whereas that for the microfine particle flotation machine averages over 120 m²/s³. In the flotation verification, the cumulative recovery rate of the fine particle flotation machine is increased by 28% compared with that of the traditional KYF flotation machine. The flotation rate is also 1.3 times that of the KYF, demonstrating stronger selectivity for fine particle concentrates. It has certain guiding significance for the resource utilization of fine particle minerals.

Preparation, characteristics and antioxidant activity of mung bean peel polysaccharides

The mung bean peel polysaccharide (MBP) extracted by hot water was chemically modified. By changing the dosage of phosphorylation reagent and acetylation reagent, three kinds of phosphorylated MBP ( P-MBP-1, P-MBP-2, P-MBP-3 ) and acetylated MBP ( AC 0.6-MBP, AC 1-MBP, AC 1.4-MBP ) with different degrees of substitution were prepared. By measuring the sugar content and substitution degree of the modified products, it was found that the amount of reagent had a certain effect on both of them. The modified products were determined by infrared spectrum and nuclear magnetic resonance. The results showed that the chemical modification was successful. The in vitro antioxidant capacity (·OH scavenging ability, O2−·clearing ability, reducing capacity, resistance to lipid peroxidation) of seven polysaccharide were measured, which manifested that chemical modification could enhance the antioxidant ability of MBP to varying degrees, and the DS also had a certain impact on their antioxidant activity. This promoted the development of mung bean peel polysaccharide functional products and the utilization of mung bean peel resources.

A new approach for extracellular RNA recovery from Rhodovulum sulfidophilum

The development of RNA-based drugs is highly pursued due to the possibility of creating viable and effective therapies. However, their translation to clinical practice strongly depends on efficient technologies to produce substantial levels of these biomolecules, with high purity and high quality. RNAs are commonly produced by chemical or enzymatic methods, displaying these limitations. In this sense, recombinant production arises as a promising, cost-effective method, allowing large-scale production of RNA. Rhodovulum sulfidophilum (R. sulfidophilum), a marine purple bacterium, offers the advantage of RNA secretion into the extracellular medium, which contains low levels of RNases and other impurities. Therefore, RNA recovery can be simplified compared to standard extraction protocols involving cell lysis, resulting in a more clarified sample and an improved downstream process. In this work, R. sulfidophilum was transformed with a plasmid DNA encoding pre-miR-29b-1, which is known to be involved in the Alzheimer's disease pathway. After production, the pre-miR-29b-1 was recovered through different extraction methods to verify the most advantageous one. A protocol for extracellular RNA recovery was then established, revealing to be a simpler and less time-consuming method, reducing around 16 h in execution time and the quantity of reagents needed when compared to other established methods. The new strategy brings the additional advantage of eliminating the toxic organic compounds routinely used in intracellular RNA extractions. Overall, the optimized process described here, using isopropanol as the precipitation agent, offers a greener, safer, and faster alternative for recombinant RNA recovery and concentration, with an extracellular RNA recovery of 7 μg/mL and target pre-miRNA-29b-1 recovery of 0.7 μg/L of medium.

Synthesis and optimization of long-chain fatty acids via the oxidation of long-chain fatty alcohols

Abstract The oxidation of long-chain fatty alcohols with Jones reagent to prepare long-chain fatty acids is an efficient and economical method. The by-product of chromium sulfate is electrolyzed and oxidized to hexavalent chromium, which is recycled to avoid the emission of toxic by-products. In this article, the process conditions for the preparation of carboxylic acid by chromium reagent oxidizing alcohol are optimized, and the effects of various reaction conditions on the experiment are explored. Innovatively, the reaction was optimized by reverse addition. The two synthesis methods are compared by orthogonal experiments, and the optimal reaction conditions for oxidation are determined: the organic solvent of alcohol is added to the acidified Jones reagent by reverse addition; the amount of chromium reagent is 1.5 times the required stoichiometry; the chromium concentration in chromium reagent is 2.5 mol·L−1; the reaction temperature was lower than 30°C and the reaction time was 4 h. Under the optimized reaction conditions, the formation of by-products was effectively inhibited, and the conversion rate of alcohol oxidation to carboxylic acid was increased from 80% to more than 90%.

Synthesis of α-Aminonitriles via Ammonium-Catalyzed Reactions of Aminoacetonitrile.

α-Aminonitriles are not only broadly useful building blocks but also structural motifs in bioactive molecules. The Strecker reaction is one of the most widely used methods for α-aminonitrile synthesis. However, a severe drawback in Strecker reactions is the required use of a stoichiometric amount of toxic cyanation reagents. Thus, the development of a greener and widely applicable method for the synthesis of aminonitriles from readily available starting materials presents an important yet unmet challenge. We developed a general and new method for the synthesis of aminonitriles from readily available aminoacetonitrile. This method utilized off-the-shelf ammonium salts as catalysts, tolerated air and moisture, and avoided the use of cyanation reagents, which rendered it a greener alternative to the widely practiced Strecker reaction approach. We further illustrated that chiral ammonium-catalyzed asymmetric reactions of N-arylidene aminoacetonitriles could provide chiral α-tertiary and α-quaternary aminonitriles and α-aminonitriles bearing two continuous stereocenters.

Method for determining reagent optimum volume for acid treatments of carbonate reservoirs based on a pressure build-up curve method

Relevance. The need to increase the efficiency of acid treatments of carbonate reservoirs. Often, the design of treatments does not take into account the actual operating conditions of production wells, which leads to an overestimation or underestimation of the required volume of the reagent. Aim. To propose a method for determining the optimal amount of reagent for carrying out acid treatments of carbonate reservoirs in order to restore the permeability of the rock in the near-well zone, taking into account the actual operating conditions of the well, which allows rationalizing the use of subsoil user resources. Objects. Producing oil wells exploiting oil deposits in carbonate deposits of the Perm region, as well as the results of hydrodynamic studies carried out on these wells. Methods. Theoretical substantiation of the possibility of determining the zone of deteriorated permeability around the well using the pressure recovery curve graph, graphical processing of diagnostic graphs of the pressure recovery curve, mathematical calculation of technological indicators based on hydrodynamic testing data of wells, integration of inflow volumes, analysis of technological data and conditions of applicability of the methodology. Results. The authors have developed the methodology for determining the optimal volume of reagent for acid treatments of carbonate reservoirs based on the pressure recovery curve method. The optimal volume of reagent for 21 production wells was calculated. Using the example of 13 wells, the efficiency of the injected volume of reagent was determined and an overestimated volume of reagent injected into the formation was demonstrated. The authors assessed the subsoil user's costs taking into account the excess consumption of the reagent. The factors influencing the success of the method application depending on the operating conditions of the wells were identified. Criteria inappropriateness of acid treatment were identified.

Microvolumetric determination of thrombomodulin based on competitive immunoreaction using a portable glucometer.

A new method of reducing the amount of reagent and sample for determination of thrombomodulin (TM) was developed based on competitive immunoreaction using a portable glucometer (PGM). Two types of nanocomposites, TM protein-modified magnetic nanoparticles (MNPs-TM) and TM antibody-/glucose oxidase-modified gold nanoparticles (Ab-GNPs-GOx), were prepared. Their binding product, MNPs-TM-Ab-GNPs-GOx, in the microvolumetric solution was used to catalyze the oxidation of glucose, leading toa decline of the glucose content. The TM-involved competitive immunoreaction had a negative effect on the generation of MNPs-/GNPs-based nanocomposites and inhibited the catalytic oxidation of glucose. The glucose content difference in the microvolumetric solution, which was revealed by a PGM, was in proportion to the logarithm of the TM concentration from 25ngmL-1 to 2.5μgmL-1. The limit of detection was 5.7ngmL-1. Microvolumetric solution and a PGM were used in the measurement, which overcame some deficienciesof classical methods in chemo/biosensing, for example, special instrument, complicated measurement procedure, and high cost.

Evaluation of the Stability and Antibacterial Activity of Burow's and Neo-Burow's Solutions was Prepared Using Different Methods

Burow's solution is a 13% aluminum acetate solution used for treating chronic suppurative otitis media. However, multiple formulations for Burow's and neo-Burow's solutions are used as in-hospital preparations. Each formulation uses different types and amounts of reagents, and takes a different time to prepare. Thus, the ions, including aluminum ion (Al3+), and other molecules in the prepared Burow's and neo-Burow's solutions are not identical, and the pH also differs. Furthermore, details about the antibacterial activity of these preparations are unknown. This study evaluated the stability and antibacterial activity of four Burow's and two neo-Burow's solutions prepared using different methods. Preparation times ranged from 20 min to 3 d, and the pH ranged from 2.2 to 4, meaning some solutions were more acidic or more basic than the pH 3 devised by Burow. In addition, the Al3+ concentrations ranged from 0.05 to 1.51 mol/L, meaning some solutions were more concentrated or diluted than 13% aluminum acetate (0.64 mol/L). One of the Burow's solutions we prepared produced a white residue after 14 d, making it difficult to ensure stability. In addition, confirming the antibacterial activity of another Burow's solution against the test bacteria was problematic. Despite the differences in pH and Al3+ concentrations between the various Burow's and neo-Burow's solutions, the antibacterial activity was equivalent. It was considered necessary to use the basic data obtained in this study to select a formulation for each hospital. Evaluation of the antibacterial activity of each formulation in clinical settings will be a subject for future study.

Activation mechanism of CoFe2O4 nanocatalysts on different oxidants and degradation efficiency of butyl xanthate

Owing to the technological limitations associated with beneficiation technology, large amounts of flotation reagents remain in mineral processing wastewater, which is extremely detrimental to humans and the environment. In addition, direct reuse of mineral processing wastewater will adversely affect the entire mine water system. Efficient treatment of residual butyl xanthate from tailing wastewater has recently attracted increasing interest. In this study, we compared the degradation efficiencies of cobalt iron oxide (CoFe2O4) nanoparticles assisted by sulfite-activated advanced processes and persulfate (PDS) and peroxymonosulfate (PMS) advanced oxidation processes on butyl xanthate (BX) for the first time and explored possible reaction mechanisms in various systems. We conducted comprehensive experiments on the acid/alkali washed catalysts using oxidizing agents, obtaining catalysts best adapted to three degradation systems. Results of batch experiments indicated that the BX degradation rate for all the three systems exceeded 95 % within 60 min under their respective optimal conditions and that the best total organic carbon content (TOC) removal rate for these systems was 85.79 %. The effects of key factors, such as catalyst dosage, oxidant dosage, solution pH, and coexisting ions, on BX degradation were investigated. Furthermore, the active free radicals that played a major role in each system were detected via electron paramagnetic resonance (EPR). EPR results indicated that SO3·− and ·O2− were the main active species of the CoFe2O4/sodium sulfite system, ·OH and ·O2− participated in the degradation of BX in the CoFe2O4/PDS system, and ·O2− was present in the CoFe2O4/PMS system. Thus, this study promotes the application of CoFe2O4 with magnetism as a catalyst in wastewater treatment and provides new insights into the effective treatment of organic pollutants in tailing wastewater.