Amount Of Phosphorus Research Articles

Integrating best management practices with dynamic water environmental capacity for optimal watershed management

Definite setting of water quality target is a challenge when considering non-point source (NPS) pollution which demands reasonable allocation of reduction requirements. However, research on the dynamic water environmental capacity (WEC) allocation aimed at reflecting varying rainfall conditions has rarely been performed. In this study, the relationship between rainfall and WEC was explored by a stochastic algorithm, while then reduction target was allocated in a small catchment, the Three Gorges Reservoir Region (TGRR), China. Combined with the distributed model, three commonly adopted best management practices (BMPs) were located precisely. The results indicate that a specific rainfall threshold exists for water quality (84 mm/month for the study area) which reveals the comprehensive effect of rainfall flushing and dilution effect. At 90% confidence, the total phosphorus (TP) load was 1518 kg/yr, and the ideal WEC was 1194 kg/yr, with excess of 324 kg/yr. In addition, the highest amount of inflow phosphorus can be reduced by fertilizer reduction (FR) with 303.8 kg/yr. Besides, slope-to-terrace (ST) measures should be implemented mainly in the upper reaches, while FR should be preferentially considered in the lower reaches. For the small watershed dominated by agricultural NPS, this study can provide accurate water quality target settled by WEC calculation impacted by uneven rainfall and a feasible watershed management.

The Potential of Micro-Dictum Preparation in Surface Water Reclamation Subject to Strong Anthropogenic Pressure

The aim of this research was to analyze the potential of e micro-dictum preparation containing compositions of beneficial microorganisms using this product in surface water reclamation. The experiments were carried out in 2016. The scope of this research included the analysis of the physical and chemical properties of a solid preparation; tests of the microbiological parameters of micro-dictum; an analysis of the spread of microorganisms in the aquatic environment; a study of water quality with the solid preparation; and tests of the formulation in real conditions and its potential in the reclamation of surface waters. Tests on the produced formulation were carried out in the laboratory in containers and under real conditions. Laboratory tests have shown that the analyzed preparation may introduce certain amounts of nitrogen and phosphorus into the water. However, they are not important in the case of water reclamation. Analyses of the micro-dictum preparation showed that the content of lactic acid bacteria in the center of the ball is lower compared to the outer layers. The results describing an increase in the number of lactic acid bacteria correlate with a decrease in pH and oxygen dissolved in the water with the preparation. The tests showed no negative impact on changes in the physical and chemical properties of water at the site of application. Changes in physical parameters were recorded, in particular dissolved oxygen and pH at the bottom, where the greatest microbiological activity occurred.

Variations in the level of available phosphorus with changes in the status of water-soluble organic matter derived from different organic materials in soil

The combined application of organic material and phosphorus fertilizer is an effective method to enhance phosphorus use efficiency for plant growth. This is partly because the presence of water-soluble organic matter (WSOM) derived from different organic materials can enhance the level of available phosphorus in the soil; however, it is poorly understood how this level varies with changes in the WSOM status (i.e., decomposed, dissolved, and retained) in the soil depending on WSOM types. This study aimed to (i) understand how changes in the WSOM status enhances the available phosphorus level in the soil, and (ii) determine the WSOM type that contributes to such enhancement. The incubation test showed that fractions of 73%–92% and 8%–27% of WSOM-derived organic carbon were retained and dissolved, respectively, at the beginning of incubation, while 31%–45% was decomposed during the incubation period. The WSOM derived from cattle manure compost (CM) and sewage sludge compost (SSC) that was initially retained was maintained until the late stage of the incubation test, whereas that derived from hydrothermal decomposed liquid fertilizer (HDLF) was rapidly desorbed during the first 14 days of the incubation period. The available phosphorus level was higher under the combined application of CM- and SSC-derived WSOM than under the single phosphorus application throughout the incubation period, while it was high only during the first 3 days of incubation under the application of HDLF-derived WSOM. The amounts of retained organic carbon at each sampling point during the incubation period compared to those at the beginning were positively and linearly correlated to the available phosphorus levels that were enhanced by the WSOM present in the soil. This study for the first time provides quantitative experimental evidence that 1) the longer the WSOM continues to be retained, the higher the amount of available phosphorus remaining in the soil, and 2) the available phosphorus level decreases with WSOM sorption or decomposition. Furthermore, it was shown that highly humified WSOM has a great potential for the maintenance of higher available phosphorus levels. This study provides the insight that a combined application of highly humified organic materials with a chemical fertilizer is necessary for not only cost effective but also sustainable fertilization design.

Effect of Different Levels of Nitrogen, Phosphorus and Potassium Fertilizers on Yield of Cucumber (Cucumis sativus L.) Under The Climatic Conditions of Takhar Province

The experiment was carried out in the research farm of Agriculture Faculty, Takhar University under the title of the effect of nitrogen fertilizer with different amounts of phosphorus and potassium fertilizers on cucumber yield under the climatic conditions of Takhar Province, Afghanistan. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications and four treatments. Treatments T1 without of any fertilizer, T2 nitrogen 80 kg ha-1, phosphorus fertilizer 100 kg ha-1, and potassium fertilizer 85 kg ha-1. T3 nitrogen fertilizer 80 kg ha-1, phosphorus fertilizer 120 kg ha-1 and potassium fertilizer 95 kg ha-1. T4 nitrogen fertilizer 80 kg ha-1, phosphorus fertilizer 140 kg ha-1 and potassium fertilizer 105 kg ha-1. In the order of information related to fruit length, fruit diameter, weight per fruit, weight per plant, fruit yield per hectare in tons was registered. The maximum fruit length (19.39 cm), fruit diameter (4.80 cm), weight per fruit (184.87 gr), fruit weight per plant (2.86 kg) and performance per hectare (61.37 tons) in T4 with nitrogen fertilizer 80 kg ha-1, phosphorus fertilizer 140 kg ha-1 and potassium fertilizer 105 kg ha-1 were found, while the lowest recorded data was from T1 i.e. control. From this experiment, it can be said that the use of NPK fertilizer with the use of 80 kg of nitrogen fertilizer ha-1, 140 kg of phosphorus fertilizer ha-1 and 105 kg of potassium fertilizer ha-1 for cucumber cultivation caused better growth and performance in the field of Takhar University.

Evaluation of body calcium and phosphorus composition of the Cobb 700 genotype during growth

ABSTRACT 1. The amount of calcium and phosphorus in the empty, feather-free bodies of Cobb 700 breed males and females was measured from hatch to 15 weeks of age. 2. A four-phase ad libitum feeding programme was used to feed 400 chicks of each sex. Feeds contained commercial levels of calcium and phosphorus which met or exceeded the requirements for energy, protein and amino acids. All birds were weighed weekly. Ten birds per sex were sampled at 0, 7, 14, 28, 42, 56, 70, 84 and 105 d of age. Defeathered birds were minced, freeze-dried and then analysed for protein, ash, calcium and phosphorus content. 3. Amounts of Ca in males and females at 105 d averaged 54 and 37 g, respectively, and 105 d P content in males and females averaged 35 and 27 g, respectively. The allometry of ash, Ca and P with empty, feather-free body protein was assessed. Ash was isometric with body protein, while an allometric exponent greater than 1 was established for Ca and P with body protein. 4. The allometric models and means for Ca and P relative to body protein were compared with models and means found in the literature. Comparison of the Ca/protein ratios with previous data suggested that modern broilers may not reach their potential bone mineral deposition with current dietary guidelines, even when growth rates were maximised. 5. Theoretical allometric relationships between Ca and P in bone and bone-free body and body protein have now been proposed. The allometric relationship between Ca and P and empty, feather-free body protein offers a suitable way to model the growth of these minerals in the broiler body.

Phosphorus Restriction Prevents Rapamycin-Induced Kidney Damage in Rats

Introduction: There are conflicting reports about the effect or rapamycin on the kidneys. Rapamycin is known to promote phosphaturia that may be associated to renal injury. Methods: Detailed histopathological studies were performed on the kidneys of rats with normal (control) and reduced (Nx) renal mass that were treated with rapamycin (1.3 mg/kg for 22 days) or placebo. The effect of rapamycin was also evaluated in control and Nx rats fed different amounts of phosphorus: 0.6% P (NP), 1.2% P (HP), and 0.2% P (LP). Quantitative scores of kidney lesions were obtained for interstitial nephritis (IN), tubular damage (TD), and nephrocalcinosis (NC). Results: When compared with placebo, rapamycin administration to Nx rats resulted in significant increases in IN (4.17 ± 0.74 vs. 1.51 ± 0.53%) and TD (14.45 ± 1.51 vs. 8.61 ± 1.83%). Rapamycin also increased NC both in control (0.86 ± 0.23 vs. 0.14 ± 0.06%) and Nx (0.86 ± 0.32 vs. 0.15 ± 0.14%) rats. In control rats receiving rapamycin, feeding HP aggravated IN (3.25 ± 0.48%), TD (22.47 ± 4.56%), and NC (3.66 ± 0.75%), while feeding LP prevented development of any renal lesions. In Nx rats treated with rapamycin, HP intake also increased IN (8.95 ± 1.94%), TD (26.86 ± 3.95%), and NC (2.77 ± 0.60%), whereas feeding LP reduced all lesions to lower levels than in rats fed NP. Rapamycin treatment increased fractional excretion of P (FEP), and an excellent correlation between scores for renal lesions and FEP was found. Conclusion: Rapamycin has deleterious effects on kidney pathology causing lesions that are located mainly at tubular and tubulointerstitial level. Rapamycin-induced kidney damage is more evident in rats that already have decreased renal function and seems to be related to the phosphaturic effect of the drug. Dietary P restriction prevents kidney damage in rats treated with rapamycin.

Acoustical characterization and modelling of the effects of eco-friendly flame retardant treatments on hemp fiber stacks

Vegetal wools are a bio-based insulator which building applications are limited due to their flammability. To overcome this problem while respecting their bio-based nature, it seems relevant to identify and apply an environmentally friendly fireproof treatment. However, the impact of this treatment on the acoustic performance of the vegetal wools is not examined in the literature. The objective of this work is to evaluate the efficacy of two phosphorus-based fireproof treatment methods i.e., impregnation and pulverization, and their impact on the acoustic properties of hemp fiber stacks. To assess the efficacy of the fireproof treatment, the amount of phosphorus grafted and the thermal degradation of the hemp fibers were evaluated through inductive coupled plasma (ICP) and thermogravimetric analysis (TGA), respectively. On the acoustic side, the impedance tube test results showed that the treatment can negatively impact the acoustic performance of hemp fiber samples, by the reduction of the sound absorption coefficient. To better understand the effects of treatments on the porous structure characteristics of the samples, parameters such as porosity, resistivity, tortuosity, etc. are determined and the acoustic absorption of the stacks is simulated using the JCAL modelling method.

Impact of Calcium and Phosphorus Levels on Optical Deterioration in Primary and Secondary Intraocular Lens Calcification.

The purpose of this study was to investigate the impact of calcium and phosphorus levels on optical deterioration in primary and secondary intraocular lens (IOL) calcification. A total of 18 explanted IOLs, 10 with primary, and 8 with secondary calcification, were examined. Straylight and light loss were evaluated as predictors of optical impairment. The individual amount of calcium and phosphorus was determined using thermogravimetry followed by emission spectroscopy (ICP-OES). The relationship between calcification and optical impairment was investigated. Primary calcified IOLs contained significantly higher amounts of calcium and phosphorus compared to secondary calcified IOLs (calcium P < 0.02 and phosphorus P < 0.01), translating to greater light loss and significantly higher straylight mean values. In secondary calcification, light loss and straylight were highly dependent on calcium (r² = 0.90, P < 0.001 and r² = 0.70, P < 0.01) and phosphorus (r² = 0.66 and r² = 0.65, both P < 0.02), whereas these correlations were much lower in primary calcification (all r = 0.25, P > 0.05). ICP-OES is the first methodology to precisely assess the calcium and phosphorus content in IOL calcification thus based on mass ratios allowing improved molecular characterization. Primary calcification showed higher amounts of calcium and phosphorus, translating to higher straylight and light loss and thus a higher risk for impairment of visual quality than secondary calcification. This study is the first to quantify calcification and demonstrate the relationship to optical deterioration in IOLs, substantially contributing to understand how visual impairment arises in patients with calcified IOLs.

Improvement and quality evaluation of gluten-free cake supplemented with sweet potato flour and carrot powder

Celiac disease and wheat allergy are immune-responsive small bowel disorders that respond solely to the ingestion of gluten-fortified wheat or wheat flour. As a result, for those who are allergic to wheat flour or gluten, the primary treatment is a gluten-free or wheat-free diet. The goal of this research is to develop a gluten-free cake with sweet potato and carrot powder, as well as to examine the Proximate analysis, mineral content, physical properties, and microbiological qualities determined using standard analytical procedures. To make cakes, instead of wheat flour, a combined formulation of 80 % sweet potato flour and 20 % carrot powder, 60 % sweet potato flour and 40 % carrot powder was incorporated into the cake ingredients. Quality evaluation of sample cakes was performed by physicochemical and storage properties. The proximate analysis of the data revealed that the cakes that were created contained adequate levels of protein, fiber, and carbohydrates with percentages ranging from 8.12 to 8.25 %, 3.19–4.19 %, and 36.03 to 38.25 %, respectively. Additionally, an appreciable amount of potassium, calcium, magnesium, phosphorus, and iron have been found in gluten-free cakes. There was color to the gluten-free cakes; L*, a*, and b* ranged from 42.66 to 43.60, 10.03 to 11.21, and 21.81 to 23.28, respectively. There is an assurance of a good color property because the hue and chroma values varied from 11.74 to 25.83 and from 41.02 to 78.03, respectively. The water activity (aw) value ranged from 0.854 to 0.870, which increased from 0.879 to 0.901 during storage. Furthermore, the microbial analysis revealed that the total viable count of all samples remained within the acceptable range after 8 days of storage. The findings of this study show that carrot powder and sweet potato flour can be used to overcome the limitations of gluten-free cakes and improve their nutritional quality.

Dependence of the hindgut disappearance of phosphorus in pigs on the quantity of phosphorus entering the hindgut based on a Meta-analysis

The objectives of the current study were to compare the difference between standardized ileal digestibility (SID) and standardized total tract digestibility (STTD) of phosphorus (P) in pigs using published data and investigate the factors that affect the hindgut disappearance of P in pigs. A total of 156 observations from 32 experiments that determined the apparent ileal digestibility and total tract digestibility of P in pigs were collected. SID and STTD of P were calculated by accounting for basal endogenous losses of P. Standardized hindgut disappearance (SHD) of P was determined by subtracting the STTD of P from the SID of P. The chi-square test was performed to investigate the association between SHD of P and categorical variables, including the use of phytase, the use of inorganic P sources, the use of corn-soybean meal-based diets, and body weight (BW) of pigs. To determine the effects of the SID of P on the SHD of P, a linear equation for the SHD of P was developed using the SID of P as an independent variable. The BW of pigs ranged from 10.0 to 104.8 kg and the SHD of P ranged from –22.8% to 39.8%. The STTD of P was greater than the SID of P (47.1% vs. 49.7%; P = 0.019). Based on the chi-square analysis, the supplementation of inorganic P sources tended to result in a higher occurrence of a positive value for the SHD of P (P = 0.079). In addition, the occurrence of a positive value in the SHD of P was lower when the BW of pigs was below 30 kg. However, as the BW of pigs increased, the occurrence of a positive value in the SHD of P increased (P = 0.061). A regression analysis of the SHD of P against the SID of P in pigs indicated that the SHD of P decreased as the SID of P increased in pigs (r2 = 0.17; P < 0.001). In conclusion, the STTD of P is greater than the SID of P in pigs, and the SHD of P depends on the diet composition, the amount of P entering the large intestine, and the BW of the pigs.

Features of hear microelement composition in children with type 1 diabetes mellitus

Objective: Assessment of the characteristics of the microelement composition of the hair of children with type 1 diabetes mellitus (T1DM) to develop recommendations for diet correction. Material and methods. The study involved children with T1 DM (n=28) and practically healthy children from the comparison group (n=56) aged 3-5 years living in the Omsk region. The elemental composition of hair was analyzed by atomic emission and mass spectrometry with inductively coupled argon plasma. The research materials were subjected to statistical processing using nonpara-metric criteria. Results. The elemental profile of hair of children with T1 DM has been determined. Significant differences were found in the amounts of microelements in hair: lower values of beryllium (p=0.0001), chromium (p=0.003), lithium (p=0.005), vanadium (p=0.005), selenium (p=0.043), and large amounts of cuprum (p=0.025), phosphorus (p=0.001) in the hair of children with diabetes in comparison with the group of practically healthy ones. In the group of children with T1DM, there was a smaller proportion of people with insufficient magnesium (p=0.003), phosphorus (p=0.001), and cuprum (p=0.001). The severity of mineral metabolism disorders (MMD) was less in the group of children with T1DM: 53.6% of children were classified as having no MMD or having minor MMD. Conclusions. The elemental composition of the hair of children with T1DM was characterized by higher amounts of phosphorus and cuprum, lower content of chromium, selenium, beryllium, lithium and vanadium. The proportion of children with insufficient supply of magnesium, phosphorus, cuprum was higher in the comparison group. The degree of MMD in the group of children with T1 DM was generally less.

Фосфатмобилизующие микроорганизмы сельскохозяйственных растений аридных экосистем Астраханской области

Crop productivity and yield are negatively affected by the deficiency of phosphorus in agricultural soil. Phosphate fertilizers are widely used to improve crop yields globally. Therefore, there is an urgent need to increase plant-available phosphorus in soil using feasible environmentally friendly technologies. Isolation and screening of strains with increased phosphate-solubilzing activity are necessary stages of research to intensify the phosphorus nutrition of plants by presowing inoculation of seeds with effective microorganisms. 50 isolates of microorganisms (49 bacterial and 1 yeast) were isolated from the rhizosphere and rhizoplane of agricultural plants in arid ecosystems of the Astrakhan region. Bacterial isolates are morphologically represented by Gram-positive spore-forming (3 pcs.) and non-spore-forming (43 pcs.) rod-like bacteria and cocci (3 pcs.). Preliminary screening of phosphate solubilization by isolates on solid nutrient media revealed a group of 6 isolates with maximum specific phosphate mobilization (the specific phosphate mobilization coefficient – Kr –value was 2.0–6.9 units) and the ability to reduce the pH of the medium. Determination of the dynamics of mobile phosphate content allowed us to identify an isolate capable of maintaining the mobile phosphorus amount at up to 6 μmol/ml and lowering in pH up to 4.73 in the medium. The studied isolate should be recommended as the basis of a biological preparation for optimizing the mineral nutrition of plants.

Enhanced phosphate removal from aqueous environments using three-dimensional La-doped carboxylic carbon nanotubes/alginate: Performance and mechanisms

The excessive amounts of phosphorus (P) discharged and usage have caused eutrophication and algal blooms, which seriously jeopardize the environment even the human health. In this study, carbon nanotubes (CNTs) served as carriers to develop a lanthanum-based sodium alginate hydrogel (La-CNT-COOH/SA) aimed at efficiently removing phosphate from wastewater. Characterization results confirmed successful deposition of La(OH)3 nanoparticles onto CNT-COOH. The optimal adsorption efficiency of La-CNT-COOH/SA hydrogels occurred at pH 4, with a maximum adsorption capacity of 54.4 mg/g under an initial phosphate concentration of 60 mg/L. Batch experiments demonstrated that La-CNT-COOH/SA performed well across a favorable pH range and exhibited high tolerance to common coexisting ions during phosphate adsorption. Adsorption isotherms indicated a dominance of both physical and chemical mechanisms in phosphate removal by La-CNT-COOH/SA. At elevated phosphate concentrations, the adsorption process followed quasi-second-order kinetics, primarily driven by chemical adsorption. Multi-instrument characterization emphasized that the substantial loading of La(OH)3 on CNT-COOH significantly contributed to adsorption, alongside crosslinked lanthanum ions on sodium alginate and abundant hydroxyl groups. Mechanisms of adsorption by La-CNT-COOH/SA encompassed electrostatic interactions, surface precipitation, and in-sphere complexation (La-O-P). These findings on fabrication, properties, and adsorption mechanisms of the phosphate-removal hydrogel lay a theoretical foundation for applying biomass-based materials in large-scale remediation practices.

Selective Epitaxy of Tensile, Highly Doped SiP for Planar NMOS FD-SOI Devices

The epitaxy of sources and drains is a critical enabler for enhanced transistor performances. In N-type MOS devices, selective SiP epitaxy can simultaneously yield (i) very high levels of electrically active dopants which is crucial for contact resistance minimization and (ii), tensile strain in Raised Sources and Drains (RSD) on each side of Si FD-SOI channels. This study present process solutions in order to benefit from large amounts of phosphorus in a silicon lattice and have thereby tensile strain, while maintaining excellent crystal quality, good surface morphology and a low electrical resistivity. We use here industrial precursors widely used in manufacturing, e.g. a [SiH2Cl2 + PH3 + HCl + H2] chemistry, for the selective deposition, in the 600°C-700°C range, of SiP in conventional epitaxy reactors. We achieved phosphorous concentrations as high as 4.6×10²¹ cm-3 (9.3%) in blanket SiP layers while preserving good crystal quality. In FD-SOI devices, we succeeded in having a selective process yielding rather smooth and free of defects Si:P films with 1.6×10²¹ cm-3(3.2%) of P.

Assessment of Physico-Chemical Properties of Biogas Slurry as an Organic Fertilizer for Sustainable Agriculture

Chemical fertilizers have been extensively used for growing crops and controlling plant diseases, but they pose potential hazards to the environment, soil health, plants, and people. The current world situation highlights the need to implement eco-friendly agricultural practices for sustainable crop production. Using environmentally friendly manure, such as biogas slurry, can help reduce the negative effects of chemical fertilizers. Biogas slurry is an efficient waste material and organic fertilizer, making it an ideal supplement for sustainable crop production and waste management. An experiment was conducted at IARI, New Delhi, to explore the nutrient potential of biogas slurry. The main objective of this study was to assess biogas slurry's physico-chemical characteristics and nutrient contents. Samples of biogas slurry were collected in three replications and analyzed using standard methods for macro and micronutrients. The data revealed that biogas slurry has a pH of 7.2-8.5, EC of 1.06 to 1.12 dS/m, and organic carbon content of 41.7 to 45.8%. In terms of fertility, it contains significant amounts of nitrogen (1.98-2.17%), phosphorus (0.97 to 1.15%), and potassium (1.98 to 2.17%). Additionally, biogas slurry contains micronutrients such as Zn (0.023-0.027 ppm), Cu (0.005-0.009 ppm), Fe (0.32-0.38 ppm), and Mn (0.089-0.094 ppm). Statistical analysis using ANOVA and Post Hoc tests indicated that the mean data values among all three replications do not differ significantly. Therefore, it can be concluded that the nutritive value of biogas slurry is sufficient to reduce the reliance on chemical fertilizers in agriculture. It represents an optimal long-term organic remedy for developing fertile soil, ensuring enduring agricultural productivity, and mitigating the negative environmental impacts associated with waste management.

Nitrogen reducing mechanism by microporous aeration based on microbial population characteristics: water temperature factor

ABSTRACT The formation of black odour water is primarily attributed to the elevated concentration of organic pollutants, along with an excessive amount of nitrogen and phosphorus, ultimately leading to an anoxic aquatic environment. The water temperature influence mechanism on black-odorous water restoration by microporous aeration is still lacking depth study. This paper selected (15–18) ℃ (spring and autumn), (22–25) ℃ (summer), (8–11) ℃ (winter) as temperature conditions, and investigated temperature influence on nitrogen reduction. Researches showed that: (1) The removal rates of COD, NH4 +-N and TN were significantly positively correlated with temperature (r = 0.99, 0.96, 0.97), the lowest removal rates were 83.16%, 95.68%, 58.7% ((8–11) ℃), the highest values were 92.67%, 98.27%, 70.96% ((22–25) ℃), respectively. (2) At a temperature range of 22–25°C, the microbial community exhibited the highest levels of abundance, diversity, and uniformity. Notably, Proteobacteria dominated this temperature range with a relative abundance of 79.72%. Furthermore, temperature positively correlated with the majority of dominant bacterial species, suggesting that conditions at 22–25°C are highly conducive to the growth of most bacterial communities. Among these, Limnohabitans, Alsobacter, and Candidatus_Aquirestis, which possess key functions in denitrification and nitrogen removal, displayed significantly higher abundances. It explains the positive correlation between temperature and removal rates of COD, TN and NH4 +-N from microbial population’s perspective. Thus, the best temperature for repairing black-smelly water is (22–25) ℃. This study provides technical reference for mechanism research and practical application of microporous aeration.

Mechanism of microbial action of the inoculated nitrogen-fixing bacterium for growth promotion and yield enhancement in rice (Oryza sativa L.)

The use of nitrogen-fixing bacteria in agriculture is increasingly recognized as a sustainable method to boost crop yields, reduce chemical fertilizer use, and improve soil health. However, the microbial mechanisms by which inoculation with nitrogen-fixing bacteria enhance rice production remain unclear. In this study, rice seedlings were inoculated with the nitrogen-fixing bacterium R3 (Herbaspirillum) at the rhizosphere during the seedling stage in a pot experiment using paddy soil. We investigated the effects of such inoculation on nutrient content in the rhizosphere soil, plant growth, and the nitrogen-fixing microbial communities within the rhizosphere and endorhizosphere. The findings showed that inoculation with the R3 strain considerably increased the amounts of nitrate nitrogen, ammonium nitrogen, and available phosphorus in the rhizosphere by 14.77%, 27.83%, and 22.67%, respectively, in comparison to the control (CK). Additionally, the theoretical yield of rice was enhanced by 8.81% due to this inoculation, primarily through a 10.24% increase in the effective number of rice panicles and a 4.14% increase in the seed setting rate. Further analysis revealed that the structure of the native nitrogen-fixing microbial communities within the rhizosphere and endorhizosphere were altered by inoculation with the R3 strain, significantly increasing the α-diversity of the communities. The relative abundance of key nitrogen-fixing genera such as Ralstonia, Azotobacter, Geobacter, Streptomyces, and Pseudomonas were increased, enhancing the quantity and community stability of the nitrogen-fixing community. Consequently, the nitrogen-fixing capacity and sustained activity of the microbial community in the rhizosphere soil were strengthened. Additionally, the expression levels of the nitrogen absorption and transport-related genes OsNRT1 and OsPTR9 in rice roots were upregulated by inoculation with the R3 strain, potentially contributing to the increased rice yield. Our study has revealed the potential microbial mechanisms through which inoculation with nitrogen-fixing bacteria enhances rice yield. This finding provides a scientific basis for subsequent agricultural practices and is of critical importance for increasing rice production and enhancing the ecosystem services of rice fields.

EXAMINATION OF THE COMPOSITION OF WHEY FROM MIXED CHEESE

In recent years, the ingredients of milk represent a functional food, because their use has a great effect on health. Whey is a byproduct of cheese production, which was previously considered waste. Whey proteins strengthen the immune system by helping the body produce the antioxidant glutathione, which protects against free radicals, pollution, toxins and infections. In this research we examine the composition of whey (minerals: Ca, K, Fe and P, total proteins and albumins) from mixed cheese from the "Ideal Shipka" dairy factory - Bitola. All parameters were determined spectrophotometrically, spectrophotometric, photometric - colorimetric and nephelometric methods were applied to prove the amount of certain components present in the whey composition. From the obtained results we can see that whey has the highest amount of total proteins, it contains 17.6 g/L, from which amount of albumen is 1,99 g/L. Of the minerals, the highest value was obtained for potassium 26,6 mmol/l, followed by calcium 9,47 mmol/l, amount of phosphorus was 9,39 mmol/l, while iron is found in a very small percentage 11,74 µmol/l. From the research we can conclude that whey contains a large percentage of total proteins, and of the minerals potassium. Thus, whey can be used as a dietary supplement for protein and potassium and it is attractive product for both the food and pharmaceutical industries and consumers.

Superlubricity of multilayer titanium doped DLC coatings by using low SAPS organic additives in base oil

This study systematically investigates the tribological performance of titanium doped diamond-like carbon monolayer and multilayer coatings with superior mechanical properties. The coatings were lubricated in poly alpha olefin grade 4 (PAO4) base oil, containing three concentrations of two organic, ashless, and sulfur free (low SAPS) antiwear, and extreme pressure additives with different amounts of phosphorus and nitrogen (Duraphos® 178 and Duraphos® OAP). The multilayer Ti-doped DLC coatings exhibited superior tribological performance compared to uncoated steel, undoped DLC, and monolayer Ti-DLC coatings. They exhibited coefficient of friction values which were in the range of 0.013 and 0.006 (superlubricity) for formulations with Duraphos® OAP. Duraphos® 178 significantly lowered the specific wear rates of both the monolayer and multilayer coatings.

Removal mechanism of phosphorus in water by calcium hydroxide modified copper tailings

With the development of industry and agriculture, eutrophication caused by increasing amounts of phosphorus in the environment has attracted people's attention. On the other hand, copper tailings (CT) is a kind of solid waste with large quantity, large area, and easy to cause groundwater and soil pollution. CT is also a potential resource because of its large specific surface area. CT is intended to be used as an adsorbent for removal phosphate in water, but trace heavy metals and a small amount of phosphate in CT may bring negative effects. Calcium hydroxide (Ca(OH)2) was used to modify CT (CCT), hoping to fix the heavy metals and phosphate in CT at the same time. It was found that the removal capacity of CCT was significantly higher than that of CT. The process of phosphate removal by CCT involves electrostatic sorption and surface precipitation, and there is a synergistic effect between CT and Ca(OH)2. The phosphate removal rate of CCT-0.4 increased with the increase of pH value under alkaline conditions. The XRD patterns of phosphate sorption by CCT mean that Ca3(PO4)2, Ca5(PO4)3(OH) and AlPO4 exist in CCT after phosphate removal, indicating that surface precipitation occurs during the removal process. In summary, the removal mechanism of phosphate by CCT is mainly electrostatic attraction and surface precipitation.