Amount Of Human Serum Albumin Research Articles

Effect of hyperdynamic therapy on cerebral ischaemia caused by vasospasm associated with subarachnoid haemorrhage.

Ten patients who developed neurological deficits associated with angiographically proven cerebral vasospasm caused by ruptured aneurysm were treated with hyperdynamic therapy induced by administration of a large amount of human serum albumin. No vaso-active drugs were administered. Cardiopulmonary function and intracranial pressure were monitored during the treatment. Marked improvement of neurological function was observed in all cases. Nine patients recovered completely without any neurological residual following treatment. The degree of the improvement observed during treatment closely correlated with the decrease in total peripheral resistance. Infusion of albumin did not cause elevation of intracranial pressure. It was concluded that the hyperdynamic therapy induced by administration of albumin has a dramatic effect on the ischaemic cerebral insult caused by vasospasm. It is postulated that the main effect of this treatment is produced by cerebrovascular dilatation.

The detection of antigens in immune complexes.

A procedure is described for the purification of soluble immune complexes (IC) from biologica fluids and for the detection of antibody-bound antigen. A model IC, prepared with various amounts of human serum albumin (HSA) and constant amounts of anti-HSA, gave a 44% recovery after gel filtration on Sephacryl S-300, affinity chromatography on protein A--Sepharose, and concentration of eluates there from before analysis by sodium dodecyl sulphate--polyacrylamide gel electrophoresis. It was calculated that 20 ng of antibody-bound HSA should be detectable when polyacrylamide gels are subjected to a direct radioimmunoassay involving the use of rabbit anti-HSA, 125I-protein A, and autoradiography, thus defining the sensitivity of the procedure for detecting IC-bound antigen in a model system. The procedure has direct relevance to the examination of IC of unknown composition present in sera of individuals with various diseases.

The electrophoretic desorption of human serum albumin from an affinity chromatography matrix using a preparative desorption cell

Preparative amounts of human serum albumin (10 mg) have been electrophoretically desorbed from Cibacron Blue F3G-A-Sepharose 4B in 85% yield. For this purpose an electrophoretic desorption cell was constructed that was reusable and capable of containing larger quantities of affinity matrix.

Applicability of Coomassie brilliant blue G-250 as the staining dye for paper and cellulose acetete electrophoretic analysis of serum proteins

The staining ability of 5 dyes was compared by staining a known amount of human serum albumin spotted on a sheet of Separax, eluting the protein-bound dye, and measuring absorbancy at respective peak wave lengths. The relative color intensity of G-250 was much higher than that of R-250, Ponceau 3R, Amidoblack 10B or Procion blue. The colorimetric value of G-250 binding to serum protein fractions considerably fluctuated because of an unavoidable inaccuracy of spotting of the protein solution onto Separax. However, the relative proportion of protein fractions (expressed in percentage) showed a reasonable variation, especially when serum was diluted 5-fold with saline. The similar tendency was obtained when the stained protein fractions were measured using densitometer. A few examples of the G-250 staining method applied to paper electrophoresis of cerebrospinal fluid and urinary proteins were presented.

Cellular mechanisms of protein metabolism in the nephron. VII. The characteristics and significance of the protein absorption droplets (hyaline droplets) in epidemic hemorrhagic fever and other renal diseases.

1. The exaggeration of "hyaline droplet" formation observed in the renal lesion of epidemic hemorrhagic fever when treated with the infusion of large amounts of human serum albumin and the histochemical characteristics of the droplets so formed afford evidence towards their identification with the protein absorption droplets of experimental procedures and with those that occur in other renal diseases. 2. Protein absorption droplets (hyaline droplets) are the visible aspect of pathological modifications of a physiological process; i.e., the continuing reabsorption of plasma proteins by the proximal convolutions. 3. The mitochondria of the renal cells are directly involved in both the physiological and the abnormal reabsorption and disposal of the plasma proteins; the absorption droplets are a complex of reabsorbed proteins and mitochondrial substances and enzymes; they result whenever disposal is at a rate insufficient to prevent accumulation. 4. Failure of intracellular disposal of reabsorbed protein is determined by (a) the quantitative and qualitative characteristics of the protein and (b) by the functional state of renal cell. 5. Various factors in renal disease that result in disturbances of reabsorption and of intracellular disposal, both with and without droplet formation, are described.